Objective To analyze antibacterial resistance and distribution of Acinetobacter baumanni in clinical departments,in order to provide references for hospital infection control.Methods Clinical isolates of Acinetobacter baumanni from January 2012 to July 2014 were collected.Bacterial identification and antibacterial susceptibility tests were carried out by using the VITEK 2 Compact automatic bacterial identification system,and results of resistance of Acinetobacter baumanni were analysed by using the WHONET5.0 software.Results A total of 285 strains of Acinetobacter baumanni were isolated and mainly were isolated from the intensive care unit(ICU)(accounted for 47.0%),most of the infected patients were male,and patients aged 45 years and over ac-counted for 70.5%.The resistance rate of aztreonam against Acinetobacter baumanni(71.9%)was the highest and the lowest was levofloxacin(25.7%).The resistance rates of most of antibacterials tested in this study were approximately 50%,and resistance rates of piperacillin-tazobactam and imipenem was approaching 40%.Conclusion Strains of Acinetobacter baumannii are mainly i-solated from ICU and antibacterial resistance of isolates is serious.Hospitals should strengthen infection control and promote ra-tional use of antibacterials according to results of antibacterial-susceptibility test,so as to reduce antibacterial resistance.