Objective To explore the factors affecting results of alanine aminotransferase(ALT)preliminary screening by using dry chemical method in blood donors,so as to establish standards of ALT preliminary screening and reduce waste of blood. Methods A total of 21 065 blood specimen of blood donors in Blood Center of Maoming City were collected from January to June 2013.The influences of specimen hemolysis,lipidemia,different sample amount and waiting time after adding sample on the detec-tion results of ALT by using dry chemical method were comparatively analysed,and standardized procedures and established appro-priate limits of ALT preliminary screening according to the results.Results The specimen hemolysis affected test results of ALT, which was not obvious when the hemolysis rate was less than or equal to 0.5%.There was no obvious interference of lipidemia on ALT test.When the sample amount was (32±3)μL or the waiting time after adding sample was 10 to 40 seconds,the detection re-sults of ALT were acceptable.The standard limit of ALT was less than or equal to 40 U/L of female and less than or equal to 45 U/L of male.Conclusion Hemolysis,sample amount and waiting time after adding sample can influence the results of ALT detec-ted by using dry chemical method,while lipidemia had no interference on ALT test.Standardizing operation and reasonably setting normal limits standard may ensure the accuracy and stability of determination,save blood resources,further ensure blood safety, which should be worthy of wide application.

Objective To know the situation of the hepatitis C virus(HCV)infection in blood donors of Maoming City,and pro-vide the basic data for recruiting volunteer blood donors and preventing of HCV infection.Methods Blood samples were collected in Maoming blood center,and were detected by using HCV antibodies of different manufacturers and operated by different people.Du-plicate test was used to reexamine the specimens which were reactive in the initial test.Statistical analysis was performed.Results HCV prevalence of blood donors from Maoming was 0.74%.The prevalence of female was slightly higher than the male,but the difference was not statistically significant(P >0.05).The HCV prevalence of civil servant and medical personnel was relatively low-er.HCV prevalence of repeat blood donors was obviously lower than blood donors for the first time(P <0.05).Conclusion HCV prevalence of blood donors in Maoming City was in a relative low level.The risk of HCV infection in people who is younger than 35 years old and at the same time qualified to donate blood is quite low.Establish a stable volunteer blood donor group is beneficial to blood safety.It's necessary to strength the propaganda and prevention of HCV knowledge to population besides civil servant and medical personnel,which would be helpful for the control of HCV infection.