Necrobiosis lipoidica diabeticorum is a rare disease and possibly confused with another conditions such as sarcoid disease, rheumatic pimple … In its histopathological study, it must consider the histochemical methods in detecting the damage of connective fiber tissues as well as clinical signs of the disease. The gender ratio is 1/3 (male/female), the mean age of onset is 30 years old. The necrotic lipoid tissue can occur in various areas – thigh, leg, foot, hand and head skin, most commonly in the right face of the leg
diagnosis ; Necrobiosis Lipoidica ; Therapeutics

A study on the prostatic samples from 2 groups of patients with prostatic intra epithelia neoplasia, group I- patients in the post-telecommunication hospital and group II- patients in the Saint Vincent. The researcher found that there was no significant difference of ages between 2 groups, the prostatic hyperplasia frequently occurred in persons with ages of 60-65, while prostatic epitheliama frequently occurred in patients with ages of 80-85. The high grade lesion of prostatic intra epithelial neoplasia in the group I was less than this in the group II. The prostatic intra epithelia neoplasia combining with prostatic epithelioma in the group I was lower than this in group II.
Prostatic Hyperplasia ; Carcinoma

Background: CARD4/NOD1 is a member of the Caterpiller (CLR) gene family and is involved in the recognition of entero-invasive bacteria, such as Helicobacter pylori and ensures immunity responses. As a consequence, gene variability can influence the gastric inflammatory response to Helicobacter pylori. Objectives: The study was conducted to investigate the frequency of the E266K CARD4/NOD1 gene polymorphism and its relationship with Helicobacter pylori infection as well as the outcome on upper gastrointestinal endoscopy. Methods: Consecutive patients who underwent gastrointestinal endoscopy were eligible for the study. DNA was isolated from whole blood and polymerase chain reaction followed by restriction fragment length polymorphism procedure was used for genotyping. The link between endoscopic diagnosis, Helicobacter pylori infection status and genotype data was analyzed using Pearson\u2019s chi-square test and regression analysis. Results: A total of 186 patients were studied (mean age of 43 years with SD = 12.6, male was 37%). Helicobacter pylori infection rate is indifferent between patients with different variants of E266K (wildtype (n=80) 54%, heterozygotes (n=71) 65% and homozygotes (n=35) 43%; p=0.09). In patients infected with Helicobacter pylori, the prevalence of peptic ulcer identified by endoscopy was 19%, 17% and 47% for the E266K wildtype, heterozygote and homozygote variants, respectively (p=0.049). Conclusion: The E266K CARD4/NOD1 gene polymorphism carriers are at increased risk of peptic ulcer in the presence of Helicobacter pylori.
Peptic ulcer ; gene ; Helicobacter pylori

Background: Some researchers indicated that investigating MUC5AC is necessary for H.pylori infection because H.pylori is easy to locate in MUC5AC. However, there is no publication about the effect of variable number tandem repeat polymorphism of MUC5AC on H.pylori infection. Objective: To investigate the relationship between mucin 5AC variable number tandem repeated polymorphism and H.pylori infection. Subject and Method: 170 patients were studied, mean age: 43, male: 35%. Blood samples were collected from patients, who visited Can Tho General Hospital for upper gastrointestinal endoscopy. DNA was isolated from whole blood; the repeated section of the MUC5AC alleles was not cut out with a restriction enzyme (SacI). H.pylori infection was diagnosed with 14 C urea breath test. Distributions of allele fragment length were compared to H.pylori positive and negative patients. Results: 52% of patients were H.pylori infected. MUC5AC SacI-restricted allele fragment lengths ranged from 6.2 to 11.2 kbp (mean 7.9), the calculated number of repeated ranged from 170 to 380 (mean 243). Mean allele length was identical for H.pylori positive and negative patients were 7.9 kbp, and the distribution of allele fragment lengths was also similar for both groups (p=0.7). Conclusion: The number of repeats in the MUC5AC gen was highly polymorphic, but the variation in allele length was limited. The variable number tandem repeat polymorphism of MUC5AC was not associated with H.pylori infection.
H.pylori infection ; MUCSAC

Background: Several studies reported a high prevalence of vitamin B12 deficiency in some Asian countries and in Asian immigrants living in Western countries. However, there is no data regarding the prevalence of vitamin B12 deficiency in Viet Nam.\r\n', u'Objectives: The aim of this study was to determine the prevalence of vitamin B12 deficiency in patients with upper gastrointestinal symptoms in the Mekong Delta and to investigate the risk factors for vitamin B12 deficiency. \r\n', u'Methods: Blood samples were collected from patients visiting Can Tho General Hospital for upper gastrointestinal endoscopy. Serum vitamin B12 concentration was measured at the University Medical center St Radboud Nijmegen, Netherlands. \r\n', u'Results: Between September and December 2003, 216 patients (80 male, 136 female, mean age 43.2 years) were studied. Ten patients had gastric cancer, 28 peptic ulcer disease/ and 178 gastritis only or no visible pathology. Only 2 patients (0.9%) had clinical vitamin B12 deficiency (<160 pmol/l, whereas 15 patients (7.0%) had sub-clinical vitamin 812 deficiency (160-250 pmol/l). H. pylori positive patients (n=111) were more likely to have para-clinical vitamin B12 deficiency than H. pylori negative patients (odds ratio 3.7~ 95%CI 1.10-12.76). \r\n', u'Conclusions: Vitamin B12 deficiency is uncommon in the Mekong Delta. Helicobacter pylori infection is a risk factor for vitamin B12 deficiency. \r\n', u'
Vitamin B12 deficiency ; gastroenterologic symptoms

Background: Dementia is a common pathological condition that affects older people. Most causes of dementia are Alzheimer\u2019s disease and vascular dementia. Diagnosing these conditions mostly relied on clinical patterns, but some biomarkers have been mentioned as the indicators of this condition. Objectives: 1) To evaluate the alteration of some biomarkers in cerebrospinal fluid (CSF) samples from Alzheimer\u2019s patients. 2) To compare the concentration of biomarkers in CSF samples from patients with vascular dementia and Alzheimer\u2019s disease. Subjects and method: Case group involved 41 patients who were diagnosed as AD and vascular dementia based on DSM-IV criteria. 31 matched healthy people were included in control group. All subjects were given neuro-psychological tests and thorough clinical examination. Brain CT scan and MRI were done for both groups. CSF samples were taken from patients in the study group to measure levels of some biomarkers. Results. The levels of total taurine (T-tau) and phosphorylated taurine (P-tau) 181 proteins are higher in the dementia group. The concentration of Abeta-42 is significantly different between case and control groups, but similar between vascular dementia and Alzheimer\u2019s disease patients. Conclusion: Changes in biomarkers are valuable in different diagnosis of Alzheimer\u2019s disease and other types of dementia. However, findings of CSF studies have to be considered with findings from imaging studies and clinical examination.
Biomarker ; Dementia ; Alzheimer\u2019s disease

Background: Laparoscopic cholecystectomy is the first laparoscopic surgery that was performed at Cho Ray hospital in September 1992. In 2005, Quang Tri general hospital has performed successfully the first case of laparoscopic appendectomy. Objectives: To access the preliminary results of application on endoscopic surgery in Quang Tri general hospital. Subjects and method: A prospective study was conducted on 140 patients with laparoscopic surgery or retroperitoneal surgery, was performed in Quang Tri general hospital from September, 2005 to April, 2006. Results:Among 140 patients was operated, there was only one case of postoperative intestinal obstructive complication (accounted for 0.7%), no case of death. Operative aged was between 11 and 70 years old. The average surgical time was 65 minutes for cholecystectomy, 35 minutes for appendectomy, 85 minutes for ureterolithotomy, 80 minutes for gynecological diseases. 2 cases of cholecystectomy and appendectomy changed open surgery. Conclusion: Laparoscopic surgery is a safe and effective method. It can be developed in provincial hospitals. Quang Tri general hospital has successful preliminary developed the new technique.
Laparoscopy ;

To know the infection status of helminths in primary schoolchildren of southern parts of Vietnam, we performed an epidemiological study in Krong Pac district, Dak Lak Province, Vietnam. A total of 1,206 stool specimens were collected from ethnic Ede schoolchildren in 4 primary schools in 2015 and examined by the Kato-Katz technique. In addition, stool cultures were done by the Harada-Mori method to obtain hookworm larvae and then to clarify the species of hookworms infected. The results showed that the helminth infection rate was 25.0%, including 2.0% Ascaris lumbricoides, 0.33% Trichuris trichiura, and 22.8% hookworm infections. The average intensity of infection was 102.0 eggs per gram of feces (EPG) for Ascaris, 36.0 EPG for Trichuris, and 218.0 EPG for hookworms. ITS1 gene sequences of the hookworm larvae were identical with those of Necator americanus (100% homology) reported in GenBank. It has been confirmed in this study that the hookworm, N. americanus, is a dominant helminth species infected in primary schoolchildren of a southern part of Vietnam. Public health attention is needed for control of hookworm infections among schoolchildren in surveyed areas of Vietnam.
Ancylostomatoidea* ; Ascaris ; Ascaris lumbricoides ; Databases, Nucleic Acid ; Eggs ; Epidemiologic Studies ; Feces ; Helminths* ; Hookworm Infections ; Humans ; Larva ; Methods ; Necator americanus ; Ovum ; Prevalence* ; Public Health ; Trichuris ; Vietnam*

Background: There are many methods used in epidemiological studies of tuberculosis (TB) bacteria but Spoligotyping method is widely used with high accuracy, simple procedure, and carried out on strains containing a little of IS6110 segment \r\n', u'Objectives: To improve Spoligotyping technique and apply this technique to classify Mycobacterium tuberculosis\r\n', u'Subjects and method: Subjects and methods: The study included 12 medical waste samples collected at Thai Binh Hospital of Tuberculosis and 19 samples obtained from the The Hanoi Institute for Tuberculosis and Lung Diseases. Spoligo model of 31 samples were analyzed based on Spotclust and SpoIDB4 database and divided up into family and subfamily.\r\n', u'Results: Spoligotyping technique has good results with the PCR product amplified 40 cycles and presented the film in 18 hours. Obtained results in 31 medical waste samples belong to 4 families: Beijing, EAI, T1 and H3-LAM9. EAI and Beijing are dominant families with 45.16% and 38.7%), respectively. T1 and H3-LAM9 are 12.9% and 3.22%, respectively. The number of samples in the study is little but the obtained rate of different spoligo models of strains are quite diversified (41.9%)\r\n', u'Conclusion: This result is relatively appropriate with previous studies on the distribution of EAI and Beijing families in Vietnam and the world. Spligotyping technique distinguished samples belonging to Beijing or non-Beijing families, which support for clinical treatment and development of new vaccines.\r\n', u'\r\n', u'\r\n', u'
Mycobacterium tuberculosis ; Tuberculosis

Background: Recently, microplate almar blue assay has been used commonly in detecting gen related to tuberculosis drug resistance, which provides results after 5-7 days with lower cost compared to traditional methods. Objective: To evaluate the application of microplate almar blue assay for rapid detection of drug resistance of M. tuberculosis strains. Subject and Method: A microplate-based assay which uses Alamar blue reagent - an oxidation reduction dye (MABA), was used for the determination of the anti-tuberculosis drug (isoniazid-INH, rifampicine-RIF, streptomycine-STR and ethabuton=EMB) resistance of 96 M. tuberculosis strains isolated from Vietnamese patients in comparison to those obtained by conversional method. Result: MABA showed to have high sensitivity and specificity in testing the sensitivity to individual anti-tuberculosis drugs (from 82.4% for STR to 93.3% for - INH and from 82.5% for EMB to 98.4% for STR; respectively), as well as for the multi-drug resistant M.tuberculosis (86.4% of sensitivity), highly correlated with the result determined by proportion method. Conclusion: MABA reveals the advantage in shortening test time, in simple performance and lower cost compared with the conversional culture based methods.
Microplate almar blue assay ; M. tuberculosis ; drug resistance