Female ; Humans ; Male ; Reconstructive Surgical Procedures ; Thoracic Wall ; surgery

Objective: The effect of Wuwei Xiaodu Drink (WXD) on the metabolism of bacteria and the dose-effect relationship were investigated by MSPQC. Methods: The frequency shift-time curves of the growth and metabolism of Escherichia coli, Staphylococcus aureus, and Pseudomonas aeruginosa affected by WXD were obtained by using MSPQC. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were gained according to FDT and the frequency shift. The dose-effect relationship was analyzed according to the curve which represented the relationship between the concentration of WXD and FDT. The parallel test was carried out with the traditional tube dilution method. Results: The results indicated that WXD inhibited the growth of E. coli, S. aureus, and P. aeruginosa, which was dose-dependent. The higher the concentration was, the stronger the antibacterial effect became. The MBC of WXD for three kinds of pathogenic bacteria was 0.9 g/mL. The MIC of WXD for S. aureus and P. aeruginosa was 0.8 g/mL, however for E. coli was 0.7 g/mL within 24 h. Conclusion: MSPQC is a sensitive, quantitative, quick method, which could provide the process information in real time for determining the dose-effect relationship of antimicrobial Chinese materia medica.

Objective: To investigate the prophylactic and therapeutic effects of allocryptopine on experimental hepatic fibrosis. Methods: Experimental hepatic fibrosis models were induced by injection of tetrachloride in combination with the drinking of 5% alcohol to rats and Schistosoma japomicum infection to mice. The effects of allocryptopine anti-hepatic fibrosis were evaluated by comparing the liver and spleen indexes, tissue biochemical indices (ALT, AST), lipid peroxidation indices (GSH-Px, MDA, SOD), serum fibrosis indices (HA, PCIII LN), the expression level of Hyp and collagen type I, III (CoI, CoIII), and the liver pathology before and after allocryptopine intervention. Results: In CCl 4-induced liver fibrosis model rats, compared with model group, the liver index and the expression level of CoI were obviously decreased in allocryptopine prophylactic groups (P<0.05, 0.01), high-dose prophylactic allocryptopine could significantly reduce the expression level of CoIII (P<0.01), middle-dose prophylactic allocryptopine could obviously reduce the spleen index, the content of AST (P<0.05); in allocryptopine therapeutic group, the liver index, the content of ALT, and the expression level of CoIII were significantly decreased (P<0.05, 0.01). In S. japomicum-induced liver fibrosis model mice, prophylactic allocryptopine could reduce the content of ALT and the expression level of Hyp (P<0.05); the liver index, the contents of PCIII, HA, and ALT were significantly decreased in high-dose allocryptopine therapeutic group (P<0.05, 0.01), the liver index, the contents of PCIII, and HA were obviously decreased in middle-dose allocryptopine therapeutic group (P<0.05); In addition, the hepatic histopathology was also improved in varying degrees after allocryptopine intervention. Conclusion: Allocryptopine has certain effects on anti-injury for hepatocyte, ameliorating liver function, and prohibiting hepatic fibrosis.

More and more disputes have happened to confront continuously since the separation of Lonicera Japonicae Flos and Lonicerae Flos in the 2005 edition of Chinese Pharmacopoeia, however, there are no differences in the properties, flavors, and channel tropism, functions of curing, usage and dosage in the 2015 edition of Chinese Pharmacopoeia. By consulting literatures on the medical history, identification, chemical composition, pharmacological action, quality control, and other aspects of Lonicera Japonicae Flos and Lonicerae Flos at home and abroad in recent years, we explore the similarities and differences between Lonicera Japonicae Flos and Lonicerae Flos, and the key issues of quality control in order to solve the problem of the combination and separation between them, which can lay a foundation for promoting the development of traditional Chinese medicinal materials industry.

AIM: To explore the correlation between plasma insulin level and refractive development.

METHODS: Collected 293 adolescents aged 11-12 who were examined in our hospital from January to June 2019.According to the refractive power, they were divided into emmetropia group(76 cases), low and moderate myopia group(144 cases), high myopia group(35 cases), and hyperopia group(38 cases). Measure and compare the plasma insulin and related indexes(blood sugar, glycosylated hemoglobin, C-peptide levels)of the four groups of subjects on an empty stomach and 2h after a meal. Person correlation was used to analyze the correlation between plasma insulin and its related indexes and diopter. The receiver operating characteristic(ROC)curve was used to explore the diagnostic value of plasma insulin levels for refractive dysplasia.

RESULTS: The average levels of insulin, blood glucose, glycosylated hemoglobin, and C-peptide in the low and moderate myopia group and the high myopia group on an empty stomach or 2h after a meal were higher than those in the emmetropia group and the hyperopia group. The levels of insulin, blood sugar and glycosylated hemoglobin in the high myopia group were higher than those in the low and moderate myopia group(all P<0.01). Plasma insulin, blood glucose, glycosylated hemoglobin, and C peptide levels were negatively correlated with diopter 2h after a meal(r=-0.691, -0.756, -0.546, -0.311, all P<0.05). The ROC curve showed that the cut-off value of plasma insulin level 2h after meal for identifying refractive errors was 0.367, the maximum area under the curve was 0.708(P<0.001, 95% CI: 0.576-0.840), the sensitivity was 63%, and the specificity was 73.7%.

CONCLUSION: Elevated plasma insulin level may affect refractive development, the higher the level, the higher the degree of myopia. Therefore, regular high-glycemic carbohydrate diets during the growth period may lead to the development of refractive errors and permanent visual impairment.

OBJECTIVETo investigate the clinical outcomes of limited open reduction and percutaneous K-wire internal fixation for the treatment of irreducible Gartland type III humerus supracondylar fracture in children.

METHODSFrom May 2006 to October 2014, 132 patients with irreducible Gartland type III humerus supracondylar fracture were treated with reduction and percutaneous K-wire internal fixation. The reduction was performed with the guiding of surgeon's finger, and the lateral approach with periosteum torn was chosen according to the shift direction of the distal fractures. Among them, there were 82 males and 50 females with an average age of 5.8 years old(ranged from 2 to 14 years old).

RESULTSAll the patients were followed up, the duration ranged from 6 to 36 months, with an average of 13.7 months. Ninety-five patients got an excellent result, 27 good, 8 fair, and 2 poor.

CONCLUSIONSLimited open reduction and percutaneous K-wire internal fixation for the treatment of irreducible Gartland type III humerus supracondylar fracture in children has many advantages: simple manipulate, not affected by the elbow swelling, and satisfactory curative effect. It is worth popularizing in clinic.

OBJECTIVE: To evaluate clinical effects of Double-pulley dual row technique with shoulder arthroscopy in treating scapular glenoid fracture(Ideberg typeⅠ). METHODS: From July 2017 to March 2019, 8 patiens with scapular glenoid fracture (Ideberg typeⅠ) were treated with Double-pulley dual-row technique with shoulder arthroscopy, including 7 males and 1 female;5 cased of injuries in the left shoulder, 3 cased of injuries in the right shoulder;ranging in age from 22 to 56 years old; and the time from injury to operation ranged from 3 to 10 days. X-ray and CT of shoulder joint were taken before and after operation to evaluate the fracture severity and fracture healing. American Shoulder and Elbow Surgeous (ASES) and Constant- Murley scores were used to evaluate shoulder joint function. RESULTS: All patients were followed up, and the duration ranged from 12 to 24 months, and the fracture healing time ranged from 3 to 5 months. No operative site infection was found in all patients. CT scan of shoulder joint showed satisfactory reduction and no displacement. The shoulder joint function recovered well. ASES score at the latest follow up after operation ranged from 85 to 97 points, which were higher than those before operation; Constant-Murley score ranged from 83 to 96 points, which were higher than those before operation. CONCLUSION Double-pulley dual-row technique with shoulder arthroscopy is effective to fix scapular glenoid fracture of Ideberg typeⅠwith minimal tissue trauma and significant improvement of shoulder joint function.
Adult ; Arthroscopy ; Female ; Fracture Fixation, Internal ; Humans ; Male ; Middle Aged ; Scapula/surgery* ; Shoulder ; Shoulder Joint/surgery* ; Treatment Outcome ; Young Adult

OBJECTIVETo investigate the flexibility and mobility of the Bacillus thuringiensis toxin Cry1Aa.

METHODSThe graph theory-based program Constraint Network Analysis and normal mode-based program NMsim were used to analyze the global and local flexibility indices as well as the fluctuation of individual residues in detail.

RESULTSThe decrease in Cry1Aa network rigidity with the increase of temperature was evident. Two phase transition points in which the Cry1Aa structure lost rigidity during the thermal simulation were identified. Two rigid clusters were found in domains I and II. Weak spots were found in C-terminal domain III. Several flexible regions were found in all three domains; the largest residue fluctuation was present in the apical loop2 of domain II.

CONCLUSIONAlthough several flexible regions could be found in all the three domains, the most flexible regions were in the apical loops of domain II.

Bacillus thuringiensis ; Bacterial Proteins ; chemistry ; genetics ; metabolism ; Cluster Analysis ; Computer Simulation ; Endotoxins ; chemistry ; genetics ; metabolism ; Entropy ; Hemolysin Proteins ; chemistry ; genetics ; metabolism ; Models, Structural ; Mutation ; Protein Conformation ; Protein Unfolding ; Software ; Temperature

K-complex (KC) is a major component of electroencephalography (EEG) in human brain during non-rapid eye movement sleep, and therehas long been a debate on its function. Some researchers consider that KC represents a sign of arousal response of brain to internal or external stimuli, and others suggest that it is a protective response to sustain sleep. In this article we put forward a functional model of KC based on the above two views and related literatures. According to our model, KC arises from three kinds of brain rhythms (sleep cyclic, central oscillation, and cortical oscillation). Based on the results of stimulus significance assessed by sleep-information assessment center, the generation of KC is related to three different responses-total arousal, sign of being awakened, and no sign of being awakened. In conclusion, KC is a reflection of cerebral state responsive to stimuli.

Buyang Huanwu Decoction (BHD) is a famous prescription for treating cerebral ischemia stroke with qi deficiency and blood stasis syndrome. More than 500 pieces of papers were reported about this prescription each year, including the medicinal resources, material bases, pharmaceutical, pharmacological effects, clinical application, preparation reform and so on, which showed the prescription research was broad and profound. Due to the complex constituents of this prescription, currently the network pharmacologic topologic method is difficult to illuminate the material basic and pharmacological mechanism of BHD. This paper has systematically sorted and analyzed BHD related research work for nearly ten years, mainly summarized pharmacy, pharmacology, and toxicology, thereby to put forward the present key problems to be solved immediately for creating a constituent Chinese medicine or natural compound medicine. Meanwhile, combined with the previous researches of quantitative network chromatopharmology, compared with the present medicine-marketed molecular structure main in treatment of ischemic stroke, intends to discuses critical technical problems of the material bases, the connection affirmation of effective ingredients and targets etc, in order to find out solution strategy and to lay a foundation for further development of a new constituent Chinese medicine or natural compound medicine.