1000 ml per day had to be cured by operation.

OBJECTIVE: To study the effect of oxymatrine on pathological change in brain tissue of newborn mice infected by cytomegalovirus (CMV). METHODS: CMV of TCID50 was inoculated into the brain of the newborn mice, and the morphological change in the brain tissue infected by CMV was observed with light microscope and transmission electron microscope. RESULTS: In the model control group, the results showed that there were some inflammatory cellular infiltration and focal necrosis in the brain tissue of newborn mice infected by CMV. The ultrastructure change in the brain tissue showed that the nuclear membrane of cerebral neurons sunk, the chromatin deformed and fused into masses, the cytoplasm vacuolated, the endoplasmic reticulum disarranged and the Nissl's body was blurred or disappeared. After being treated with oxymatrine (50 mg/kg, ip) for 15 days, those pathological changes of the brain tissue in the newborn mice could be significantly improved. CONCLUSION: Oxymatrine has an obvious inhibition on CMV in vivo.

Burkitt lymphoma (BL) is an aggressive B-cell non-Hodgkin lymphoma which often occurs in children. The cure rate of BL is significantly increased with the wide application of short-duration and high-intensity immunochemotherapy, however, chemotherapy-related adverse reactions are still a big problem in adult patients and human immunodeficiency virus (HIV) positive patients. Meanwhile, the absence of effective immune targeting new drugs in patients with relapsed and refractory BL needs to be solved clinically. How to optimize the therapeutic regimen to reduce the chemotherapy-related adverse reactions and develop effective immune targeting new drugs is the hot spot in current research. This paper reviews the treatment progress of BL according to the 59th American Society of Hematology (ASH) Annual Meeting.

Objective To explore the presence of myeloid-derived suppressor cells (MDSC) in patients with non-Hodgkin lymphoma (NHL) and its clinical value. Methods Peripheral blood samples were collected from 69 NHL patients and 21 healthy controls admitted in Jilin Cancer Hospital from January 2014 to February 2015. Flow cytometry was conducted to identify unique cell surface markers of MDSC using antibodies against CD11b, CD33, CD14 or HLA-DR. MDSC were enriched by immunomagnetic beads, then arginase 1 (Arg-1), inducible nitric oxide synthase (iNOS) and cyclooxygenase 2 (COX-2) in which were detected by real time-PCR. In vitro, cell proliferation assay was used to test T cell function. Statistical analysis was used to explore the correlation between MDSC and clinical features. Results There were a high level of CD11b+CD14+CD33+cells in peripheral blood of NHL patients. The morphology of the cells belonged to mononuclear cells. The ratio of monocytic CD11b+CD14+CD33+cells in NHL patients was higher than those in healthy controls [(42±10) ％ vs. (34±11) ％, t= 0.300, P= 0.005]. The expressions of Arg-1, COX-2 and iNOS in CD11b+CD14+CD33+and CD11b+CD14+CD33-cells were 0.12±0.04 vs. 1.00±0.25 (t= 6.095, P=0.024), 3.03±0.45 vs. 1.00±0.78 (t= 7.766, P= 0.016) and 0.29±0.11 vs. 1.00±0.04 (t= 1.987, P= 0.209), respectively. In addition, the CD11b+CD14+CD33+cells inhibited T cell proliferation. The levels of MDSC in patients with different international prognostic index (IPI) score were significantly different (F= 2.536, P=0.049), but the levels of MDSC in patients with different sex, age, pathological type, stage, serum lactate dehydrogenase, physical status staging criteria and β2-microglobulin had no differences (all P < 0.05). Conclusions CD11b+ CD14+ CD33+ cells are characterized as MDSC in terms of higher level in NHL patients, expressing myeloid-specific proteins, and inhibiting T cell proliferation. The expression of MDSC is associated with IPI score, implying it might be a novel biomarker in clinical practice for NHL patients.

Objective To investigate the clinical features of pulmonary lymphoma and to get a better understanding of this disease. Methods Clinical data of 253 lymphoma patients in the Department of Lymphoma and Hematology in Jilin Cancer Hospital from October 2014 to March 2017 were retrospectively analyzed. The patients were divided into 30 cases of pulmonary lymphoma (lung lymphoma group) and 223 cases of non-pulmonary lymphoma (the control group). Rate assay and latex turbidimetry was used to detect lactic dehydrogenase (LDH) and β2macroglobulin (β2-MG) respectively. The expressions of programmed death 1 (PD-1), programmed death ligand 1 (PD-L1) and cytotoxic T lymphocyte-associated antigen 4 (CTLA-4) in peripheral blood CD4 +CD8 +T lymphocytes were detected by using flow cytometry. The count and measurement data of both groups were compared by using χ 2test and t test respectively. Results The patients in pulmonary lymphoma group showed secondary lesions. The proportion of smoking people in pulmonary lymphoma group was higher than that in the control group [43.3 % (13/30) vs. 24.2 % (54/223), χ 2＝ 4.964, P＝ 0.026]. The proportion of the patients in Ⅲ-Ⅳ stage in pulmonary lymphoma group was higher than that in the control group [93.3 % (28/30) vs. 57.0 % (127/223), χ2＝ 14.750, P < 0.001]. The proportion of the patients with higher international prognostic index (IPI) score in pulmonary lymphoma group was higher than that in the control group (χ2＝ 21.888, P < 0.001). The proportion of the patients with increased expression of β2-MG in pulmonary lymphoma group was higher compared with the control group [66.7 % (20/30) vs. 50.2 % (112/223), χ2＝6.682, P ＝0.091]. The proportion of the patients with the increased LDH was higher compared with the control group [63.3 % (19/30) vs. 41.5 % (86/223)], and the difference was statistically significant (χ2＝ 6.682, P ＝ 0.010). Diffuse large B-cell lymphoma (DLBCL) was the common pathological type in pulmonary lymphoma group (15 cases), followed by Hodgkin lymphoma (7 cases); imaging showed single mass or nodular type, multiple masses or nodular type, bilateral pulmonary infiltration, pleural effusion were 36.7 % (11/30), 30.0 % (9/30), 63.3 % (19/30) and 36.7 % (11/30), respectively. There were no statistical differences in the protein expression of immune check points such as PD-1, PD-L1 and CTLA-4 in both groups (all P ＞ 0.05). Conclusions Pulmonary DLBCL should be considered a secondary disease, but not a primary lesion. Smoking history is a risk factor for lymphoma patients with pulmonary involvement. Pulmonary lymphoma is similar to other extra-nodal lymphoma with high IPI scores, advanced stage and elevated LDH.

Objective:To study on the effects of micro-plasma radio frequency technology on guinea pig skin pigmentation animal model induced by narrow band ultraviolet B (NB-UVB).Methods:We selected ten healthy guinea pigs (common grade), removed the brown hair of the guinea pig and established UVB to induce the skin pigmentation model of guinea pig. The pigmentation zone was randomly divided into four groups: Group A was used as blank control with no treatment and Groups B , C and D were treated with micro-plasma radio frequency technology using different energy parameters. Group B treatment parameters were as follows: output power was 10 watts, and treatment energy was 0.06J. Group C treatment parameters were as follows: output power was 20 watts, and treatment energy was 0.12J. Group D treatment parameters were as follows: output power was 30 watts, and treatment energy was 0.18J. They were compared before treatment and at 2, 4, and 6 weeks after treatment, based on melanin cell staining (epidermis separation-dopa staining) and melanin granules dyeing method (Masson-Fontana). Immunohistochemical staining (c-kit protein) was used to observe the tyrosinase activity in the melanocytes, melanin granule percentage area of the skin area, and the change in melanin cell activity. SPSS13.0 statistical software was used for statistical analysis of the experimental data. The significance of the difference ( P< 0.05) was measured. Results:The results showed that micro-plasma radio frequency (RF) technology treatment could reduce the tyrosinase activity in the melanocytes, 2, 4 and 6 weeks after the end of treatment: Groups B, C and D were lower than group A, and the difference was statistically significant ( P<0.05), and as the treatment energy increased, the speed of reduction was increased. Micro-plasma radio frequency technology could reduce skin pigmentation and decrease the amount of epidermal melanin granules, 2 and 4 weeks after the end of treatment: Groups B, C and D were lower than Group A; 6 weeks after the end of treatment, only Group D was lower than Group A, with statistically significant differences ( P< 0.05). An increase in the energy of the micro-plasma radio frequency sped up the decline in the amount of epidermal melanin granules.The number of c-kit positive cells was not increased in this experiment. Two weeks after the end of treatment, the number of c-kit positive cells was higher than those of Groups B, C and D. There were no statistically significant differences in Groups B, C and D ( P<0.05). No c-kit positive cells were found in Groups A, B, C and D at 4 and 6 weeks after the irradiation. Conclusions:Micro-plasma radio frequency technology could accelerate the fading of guinea pig skin pigmentation. It is effective to improve pigmentation of guinea pig skin after NB-UVB irradiation.

Objective:To study on the effects of micro-plasma radio frequency technology on guinea pig skin pigmentation animal model induced by narrow band ultraviolet B (NB-UVB).Methods:We selected ten healthy guinea pigs (common grade), removed the brown hair of the guinea pig and established UVB to induce the skin pigmentation model of guinea pig. The pigmentation zone was randomly divided into four groups: Group A was used as blank control with no treatment and Groups B , C and D were treated with micro-plasma radio frequency technology using different energy parameters. Group B treatment parameters were as follows: output power was 10 watts, and treatment energy was 0.06J. Group C treatment parameters were as follows: output power was 20 watts, and treatment energy was 0.12J. Group D treatment parameters were as follows: output power was 30 watts, and treatment energy was 0.18J. They were compared before treatment and at 2, 4, and 6 weeks after treatment, based on melanin cell staining (epidermis separation-dopa staining) and melanin granules dyeing method (Masson-Fontana). Immunohistochemical staining (c-kit protein) was used to observe the tyrosinase activity in the melanocytes, melanin granule percentage area of the skin area, and the change in melanin cell activity. SPSS13.0 statistical software was used for statistical analysis of the experimental data. The significance of the difference ( P< 0.05) was measured. Results:The results showed that micro-plasma radio frequency (RF) technology treatment could reduce the tyrosinase activity in the melanocytes, 2, 4 and 6 weeks after the end of treatment: Groups B, C and D were lower than group A, and the difference was statistically significant ( P<0.05), and as the treatment energy increased, the speed of reduction was increased. Micro-plasma radio frequency technology could reduce skin pigmentation and decrease the amount of epidermal melanin granules, 2 and 4 weeks after the end of treatment: Groups B, C and D were lower than Group A; 6 weeks after the end of treatment, only Group D was lower than Group A, with statistically significant differences ( P< 0.05). An increase in the energy of the micro-plasma radio frequency sped up the decline in the amount of epidermal melanin granules.The number of c-kit positive cells was not increased in this experiment. Two weeks after the end of treatment, the number of c-kit positive cells was higher than those of Groups B, C and D. There were no statistically significant differences in Groups B, C and D ( P<0.05). No c-kit positive cells were found in Groups A, B, C and D at 4 and 6 weeks after the irradiation. Conclusions:Micro-plasma radio frequency technology could accelerate the fading of guinea pig skin pigmentation. It is effective to improve pigmentation of guinea pig skin after NB-UVB irradiation.