Objective To investigate the expression of protease activated receptors(PARs)in mast cells.Methods Reverse transcription polymerase chain reaction(RT-PCR),flowcytometry and immunofluorescent cell staining were used to detect the expression of PARs in mast cell lines P815 and MC/9 at the levels of protein and mRNA.Results Both the P815 and MC/9 of mast cell lines expressed PAR-1,PAR-2,PAR-3 and PAR-4 at either protein or mRNA level.Conclusion The expression of all the four PARs in mast cells were detectable,which may be of significance for the further study on the function of PARs in mast cells.

[Objective] Establish The Measure Table of Emotion Evaluation of Female Experiment Macaque, evaluate types of emotions of the female experiment macaque, provide the criterion of quantization evaluation for the emotion disease.[Methods] To collect and analyse the expression and action of macaque by observation, to establish The Screening Table of expression, action and emotion for the female experiment macaque; to set up the Mathematics Model by none-condition logistic regression, to provide explanation for establishing the Measure Table of Emotion Evaluation of Female Experiment Macaque; to set up The Measure Table of Emotion Evaluation of Female Experiment Macaque; to check up the stability and consistence of the measure table by test-retest and Split-Half means; to check up the stability of the criterion of validity by equivalence means; to evaluate the validity by criterion-related means.[Results] The Test-Retest Reliability of the indignation, depression, and enjoyment emotion is higher than the other, the Split-half Reliability of the indignation and depression emotion is higher than the others; the stability of the validity Criterion of all emotion is higher except for the anxiety emotion. The emotion integral can reflect all the emotion change; The Criterion-related Validity of the indignation, depression, friendliness and sex emotion is higher than the others. [Conclusion] Although the validity of the Measure Table is lower in anxiety emotion measuring, the interior consistence is not very good in friendliness and enjoyment emotions; the validity and stability about the other primary emotions reach the criterion of the Measure Table.

To explore the central mechanisms of anger emotion and the effects of Chinese herbal medicines for regulating liver qi on the anger emotion and the expression level of 5-hydroxytryptamine 3B receptor (5-HT3BR) in rat hypothalamus.

Objective To study the differential expression of hypothalamus proteinum in PMS Liver-qi invasion mouse,in order to find the evidence on pathogenesis in protein level of PMS Liver-qi invasion.Methods The spectrum of a graph on hypothalamus protein of PMS liver-qi invasion symptom was analysed by two-dimensional gel electrophoresis,protein was identified by matrix-assisted laser desorption ionization time of flight mass spectrometry(MALDI-TOF-MS).Results Comparing model group with normal control group in hippocampus were as follows: Ubiquitin carboxy-terminal hydrolase L1,Albumin 1,Aldolase 3 were down-regulated;however M2 pyruvate kinase,ubiquinol-cytochrome creductase core protein I,Calbindin 2 were up-regulated,These protein were related to the function of ubiquitin iter、intermediary metabolism enzyme、compensation.Conclusion PMS Liver-qi invasion was related to the proteinum function of ubiquitin iter、intermediary metabolism enzyme、compensation,this results hint that this six protein were tight related with central pathogenesis of PMS liver-qi reversed symptom.

Objective To investigate the clinical features of primary angiitis of the central nervous system (PACNS). Methods The clinical data of 27 patients with PACNS were analyzed. Results PACNS commonly occured in young and middli-aged adult and with acute or subacute onset. The clinical manifestations were mainly including headache,decrudescence of cortical functions,focal neurological deficits and seizures. The hypointensity signals on T1WI and T2WI were showed and could be enhanced at lesions by skull MRI. The pia vessels inflammatory lesions were revealed in brain by pathological examination. The treated with glucocorticoid and combined therapy such as anti platelet aggregation,scavenging free radicals,reducing intracranial pressure,improving microcirculation and promoting neuronic metabolism,the condition get better.Conclusions The clinical manifestation of PACNS is various. The diagnosis can be make by combining the data of clinical features,imaging and brain biopsy.The treatment with glucocorticoid can obtain better efficacy.

Objective To study the mechanism of liver-qi stagnation syndrome of rat models. Methods The binding method was used to make rat models of liver-qi stagnation syndrome and the quantitative changes of hypothalamus norepinephrine (NE), epinephrine (E), dopamine (DA), and 5-hydroxytryptamine (5-HT) before and after modeling were determined with reversed phase ion pair (RP-IP)-HPLC. Results The level of E, DA and 5-HT was remarkably increased (P

Objective To explore the test-retest reliability and correlations of the light-dark box (LDB).MethodsThe behavior of mice in LDB was recorded twice, for five minutes,with a one-week inter-trial interval.Following parameters were evaluated:latency time for the first entry into the dark area (Latence); percentage of time exploring in the light area ( Ltime％ ); percentage of squares crossing in the light area ( Lcross％ ); total number of squares crossing ( Cross ); percentage of rears in the light area ( Lrear％ ); total number of rears (Rear); total score (Total); transitions between two areas (Transition); and number of fecal boli (Fbi). Subsequently,the intraclass correlation coefficient (ICC), the Kappa agreement coefficient (Kappa) and the Pearson correlation coefficient (Pearson) were calculated for these parameters. Results ①ICC for Lrear％ ( ICC =0.5207, P＜0.01 ) showed a fair reliability. ②Kappa for all parameters indicated a poor reliability. ③Pearson displayed weak negative correlations between Transition and Ltime％ ( Pearson = - 0.4030, P ＜ 0.05; Pearson =- 0. 1490, P＞ 0. 05 ), Lcross％ ( Pearson = - 0. 4331, P ＞ 0. 05; Pearson = - 0.1893, P ＞ 0.05 ), Lrear％ ( Pearson = - 0. 4485, P ＜ 0. 05; Pearson = 0. 02845, P ＞ 0. 05 ), however, fair positive correlations between Transition and Cross (Pearson =0. 8603, P＜0.01; Pearson =0.6341, P＜0. 01 ) ,Rear(Pearson =0.4271, P＞0. 05;Pearson = 0. 7245, P ＜ 0. 001 ). Conclusion Rear is found to be stable over time, and indicated a fair reliability.There is a weak negative correlation between Transition and Ltime％, Lcross％, Lrear％, but fair positive correlations between Transition and Cross, Rear.

Objective:To probe into indexes of evaluating rhesus monkey model of stagnation of liver-Qi of prementrual syndrome(PMS). Methods:Rhesus monkey model of stagnation of liver Qi of premenstrual syndrome was developed and qualitative analysis of expression and behaviors,and serum monoaminergic neurotransmitters examined with capillary electrophoresis were used as indexes of evaluating the model.Results:The model had stability and reiteration.Rhesus monkey in premenstrual phase showed similar clinical manifestions of this disease,with serum level of monoaminergic neurotransmitters changed.Conclusion: Macroscopic and microscopic indexes for evaluating rhesus monkey model of stagnation of liver-Qi of premenstrual syndrome can be established by means of Emotion Scale of Female Experimental Rhesus Monkey.

Objective To study the general rule in the power spectrum percentage of balance when normal people standing and the differences of the power spectrum percentage between normal people and the stroke patients. Methods 30 normal people and 30 stroke patients was tested with Active Balancer EAB-100 (Sakai Iryo) open- and close-eye. The whole path length, circumference area and the percentages of power spectrum on the X, Y and Z axes were recorded. The correlation and the differences were analyzed. Results The percentages of power spectrum were not statistically correlated (P>0.05) with age, height, weight or gender. There was significant difference between two groups in the percentage of power spectrum (P<0.05). On the frequency spectrum, the ratio rised on the low and middle frequency part, especially within 0.2~2 Hz, but the ratio decreased on the high frequency part (2~10 Hz) (P<0.05). Conclusion The percentage of power spectrum can be an important reference in assessing the stroke patients' balancing functions.

Objective To establish the reference values of static balance function with Active Balancer EAB-100 in healthy Chinese adults, and to screen the valuable indices for clinical work. Methods There were 227 healthy adults measured with Active Balancer EAB-100 when standing still with eyes opened and closed. All the data recorded were analyzed with SPSS 19.0, and the weight of every parameter was obtained with principal component analysis. Results The reference values of parameters for static balance function were established in healthy Chinese adults. The loadings of whole path length, rectangle area, circumference area, effective value area, deflection center displacement X, deflection center displacement Y, rectangle area of Romberg rate and circumference area of Romberg rate were more than average. Conclusion It is need to focus on whole path length, circumference area, deflection center displacement X, deflection center displacement Y, and effective value area and circumference area of Romberg rate much more in clinical practice for the static balance assessment.