Objective To observe the anesthetic effect onset of primacaine and lidocaine in the treatment of pregnant women with acute pericoronitis of wisdom tooth.Methods.Two hundreds pregnant patients with acute pericoronitis of wisdom tooth were randomly divided into the study group and control group,100 cases in each group.The study group adopted primacaine before treatment,while the control group adopted lidocaine.The anesthetic effects were compared between the two groups.Resuits The anesthetic effect onset in the study group was faster than that in the control group,the difference was statistically significant (P＜0.01).The two groups all reached a better analgesic effect,moreover the pain degree and fear degree had no statistical difference between the two groups (P＞0.05).Conclusion The anesthetic effect of primacaine and lidocaine in the treatment of pregnant women with acute pericoronitis of wisdom tooth is favorable with high patient's satisfaction.

Objective To evaluate the efficacy of midazolam combined with remifentanil for lower eyelid blephamoplasty.Methods In a double-blind study,200 patients undergoing lower eyelid blephamoplasty were randomly and equally divided into 2 groups (100 cases each):test group (midazolam 0.05 mg/kg and remifentanil 0.5 μg/kg followed by remifentanil 0.05 μg/(kg·min) and control group (midazolam 0.05 mg/kg).Heart rate (HR),mean arterial pressure (MAP),and pulse oxygen saturation (SpO2),respiratory rate (RR),SpO2 change,operation time,recovery time,the incidence of adverse reactions and the satisfactory rate of both patients and surgeons were monitored and recorded before the medication,3 min after the medication,at beginning of the surgery,during and immediately after the surgery,and 30 min after surgery.Results Two groups of patients had no obvious difference in intraoperative MAP,HR,RR,SpO2 and postoperative recovery time (P＞0.05);the incidence of postoperative nausea and vomiting in the test group was significantly higher than that in control group (P＜0.05); Patients and surgeons satisfaction in the test group was higher than that in control group (91.3±11.6)vs (52.7±10.4) (P＜0.05).Conclusions Midazolam combined with remifentanil for lower eyelid blephamoplasty has less inhibitory effect on circulatory and respiratory functions and the patients come round fast after surgery.But there are risks of respiratory inhibition in the induce phase and nausea and vomiting in the recovery stage.

OBJECTIVETo explore the feasibility and safety of dexmedetomidine combined with remifentanil for lower eyelid blepharoplasty.

METHODS50 patients undergoing lower eyelid blepharoplasty ASA I - II were randomly divided into 2 groups (25 in each group) as observation group and control group. Dexmedetomidine (0.3 microg x kg(-1) x h(-1)) and remifentanil (0.05 microg x kg(-1) x min(-1)) were used in observation group. Local anesthesia by lidocaine (1%) combined with midazolam (0.04 mg/kg) intravenous sedation were used in control group. HR, mean arterial pressure (MAP), heart rate (HR), breathing rate (RR), pulse oxygen saturation (SpO2) change were monitored and recorded before and after the administration of the drug, as well as at the beginning of operation, during the operation, at the end of the operation, and 5 minutes after the operation. Patients sedation degree was evaluated by Ramsay. Operation time, recovery time, the incidence of adverse reactions, the satisfaction of patients and doctors were also recorded.

RESULTSPatients had no obvious difference of intraoperative MAP, RR, SpO2, operation time and postoperative recovery time between the two groups (P > 0.05). Compared with control group, HR during the operation were decreased significantly [(64.2 +/- 8.2) bpm, P < 0.05] in observation group. In control group, there were 18 cases of patients with agitation, but they endured the operation anyway. In observation group, 24 cases of patients underwent operation without agitation. The satisfaction of patients and the operation doctors (96%) in observation group were markedly higher than that in control group (P < 0.05).

CONCLUSIONDexmedetomidine combined with remifentanil is practical and safe. The effect of reducing HR should be noticed.

Adult ; Blepharoplasty ; Dexmedetomidine ; therapeutic use ; Double-Blind Method ; Female ; Humans ; Hypnotics and Sedatives ; therapeutic use ; Male ; Middle Aged ; Piperidines ; therapeutic use

Transforming growth factor-β1(TGF-β1)has become an important biological marker and therapeutic target of clinical progression of chronic kidney diseases. Sma- and Mad-related protein (Smad)is a downstream signal transduction protein of the TGF-β family. TGF-β1 activates Smad2 and Smad3 before increasing the transcription of connective tissue growth factors in the nucleus. Smad3 promotes mesangial cell proliferation,extracellular matrix accumulation,epithelial-mesenchymal transition, leading to renal fibrosis. However,Smad2 and Smad7 play a negative regulatory role by inhibiting renal fibrosis. TGF-β1 specific inhibitor (SB431542,etc.) has antifibrosis effect,most of which is in the preclinical stage. The drugs on the market that are effective for DN,such as benzodiazepines,atorvastatin, losartan,and pirfenidone,can inhibit the expression of TGF-β1,while tripterygium wilfordii,cordyceps sinensis,and berberine can delay the process of diabetic nephropathy by reducing TGF-β1 levels.

Objective To.investigate the accuracy and reliability of monocyte count of three methods.Methods The specimens classified by blood cell analyzer WBC monocytes ＜ 15％,15％-20％,＞ 20％ were taken randomly,draw 20 μ l after mixing,put in monocyte direct count dilution and count,and underwent routine smear,stained by Swiss-Gibbs and manual classified by microscope,and then compared the results of the three methods.Collected one copy each of the high,medium and low proportion of monocytes specimens,and counted 30 times by the three counting method.Results Blood cell analyzer in WBC monocytes 15％-20％,＞ 20％ had poor correlation with the results of the other two methods.Repeatability comparison of three methods,medium and low sample were better with method 1 and 2,high value sample was good with method 3.Conclusion The specimens of monocytes ≥ 15％,especially scatter plot is abnormal,must be confirmed by microscopic examination.

Objective To investigate the association betweent clinical markers of N‐terminal probrain natriuretie peptide (NT‐proBNP) ,cardiac troponin I (cTnI) and C‐reaction protein (CRP) in the patients with acute respiratory distress syndrome (ARDS) caused by severe pneumonia and to reveal the pathophysiologic process of myocardial injury after ARDS .Methods Totally 33 patients diagnosed as ARDS caused by severe pneumonia were divided into 3 groups:mild group ,moderate group and severe group according to the New Berlin definition of ARDS .Arterial blood gas analysis ,NT‐proBNP ,cTnI and CRP were observed be‐fore the treatment .Statistics were used for the analyses .Results The NT‐proBNP level in the severe group was significantly higher than that in the mild and moderate groups (P<0 .01);the NT‐proBNP level in the moderate group was also higher than that in the mild group (P< 0 .05) .Besides ,the cTnI level in the severe group was significantly higher than that in the mild and moderate groups (P<0 .01);however ,the cTnI level had no statistical difference between mild and moderate groups (P> 0 .05) .The CRP level in the severe group was significantly higher than that in the mild (P<0 .05) .Moreover ,the NT‐proBNP level of 33 patients was positively associated with the both CRP and cTnI level (P<0 .05) .Conclusion The seriously inflammatory response mediates myocardial injury probably because of ARDS .Combined NT‐proBNP and cTnI testing is important for evaluating the condition of ARDS at the early stage .

Objective:To investigate Bcl-2 and Bax gene expression in acute myelogenous Leukemia and the relationship between Bcl-2/Bax ratio and drug resistance.Method:Expression of Bcl-2、Bax was detected by immunohistochemical method while drug resistance of AML was detected by cell culture and MTT assay.Results:A high expression of Bcl-2 and a low expression of Bax were detected in AML,respectively,and the ratio between Bcl-2 and Bax was significantly higher than that in normal control (P＜0.01). The ratio in those who are resistant to chemotherapy was significantly higher than that in those sensitive to chemotherapy (P＜0.05).The responsiveness to chemotherapy of those with a high ratio was poorer than thos with a low ratio (P＜0.05).Conclusion:The alteration of Bcl-2 and Bax played certain role in the development of drug resistance in AML,the test of Bcl-2/Bax ratio may have great significance in choice of chemotherapeutic agents and also provide important information for outcome prediction.

Objective:Compare the characteristics of male and female suicide attempters with the goal of explaining the significantly higher rates of suicide attempts in women.Method:Investigators who were rigorously trained in the research schedule assessed 326 serious suicide attempters (248 women and 78 men) treated in the emergency rooms of general hospitals and conducted an examination to determine the psychiatric diagnosis (based on DSM-IV criteria).Results:Most characteristics were similar in male and female attempters: age, family financial status and characteristics, number of prior suicide attempts, method used in the attempt, suicidal intent, number and psychological effect of negative life events, and rate of mental illness. The three most frequent negative life events were the same for men and women: marital conflict, major changes in daily routines, and financial difficulties. But compared to male attempters, female attempters had lower educational achievement, were more likely to be currently married, were more likely to have been working at the time of the attempt, had younger co-resident family members, had less contact with adult family members in the month before the attempt, and were less likely to have alcohol dependence (p's all

Objective To explore the effect of glycerol enemas used in different ways on the postoperative recovery of intestinal function.Methods Seventy-two patients after abdominal surgery were randomly divided into the control group and the experiment group:in the former group glycerol enema was injected into the anus 3~4 cm and in the latter group the enema was injected into anus 15 cm using a syringe connected to suction tube.The two groups were compared in terms of first time for anal exsufflation,time for defecation and abdominal distension.Results The first time for anal exsufflation and the time for defecation were both significantly shorter than the control group and the rate of abdominal distension was lower than those in the control group(P<0.05).Conclusion Injection of enema by connecting the syringe to a suction tube may be effective in promoting the recovery of intestinal function and lower the rate of abdominal distension.

Objective: To get the information about the civil will of donating cadaver in Guangzhou. Method: Make stochastic visit to dwellers in Guangzhou.Result: Traditional concept,legal system and lack of the knowledge of donating cadaver affect the civil will most. Conclusion: More effects are needed to update the social viewpoints and strengthen the legal system.