Objective To observe the anesthetic effect onset of primacaine and lidocaine in the treatment of pregnant women with acute pericoronitis of wisdom tooth.Methods.Two hundreds pregnant patients with acute pericoronitis of wisdom tooth were randomly divided into the study group and control group,100 cases in each group.The study group adopted primacaine before treatment,while the control group adopted lidocaine.The anesthetic effects were compared between the two groups.Resuits The anesthetic effect onset in the study group was faster than that in the control group,the difference was statistically significant (P＜0.01).The two groups all reached a better analgesic effect,moreover the pain degree and fear degree had no statistical difference between the two groups (P＞0.05).Conclusion The anesthetic effect of primacaine and lidocaine in the treatment of pregnant women with acute pericoronitis of wisdom tooth is favorable with high patient's satisfaction.

We have developed an antibody-captured sandwich-type enzyme linked immunosorbant assay(ELISA) with monoclonal anti-IgA/a (human) and monoclonal anti-HBc, and determined immunogloblin A against hepatitis B core antigendgA anti-HBc) in sera of 179 cases of various types of HBV infection. IgAanti-HBc in the patients with acute hepatitis B (14 cases) and severe hepatitis (7 cases) was all positive, while in the patients with chronic active hepatitis, cirrhosis, chronic persistent hepatitis, and symptomatic carriers the positive rates were 94% (47/50 cases), 83.3% (10/12 cases), 56.5%(26/46 cases) and 4%(2/50 cases), respectively. Our observations suggested that IgA anti-HBc may reflect hepatic damage in the patients with HBV infection. Its detection may be valuable in the diagnosis and prognosis HBV infection. In addition, the activity of IgA anti-HBc in chronic hepatitis B is related to HBeAg.

AIM: To investigate 86 patients (age range, 12 to 80 years; mean?SD age 48.63?18.11) who had status epilepticus. Among them 60 patients had generalized tonic-clonic status epilepticus, 21 myoclonic clo-nictonic clonic status epilepticus 3 generatized tonic status epilepti-cus and 2 patients complex partial status epilepti-cus-psychomotor status epilepticus. METHODS: We conducted two centers, of 45 patients were given Sod. Phenobarbital im group and of 41 patients were given Diazepam Ver iv group. RESULTS: Significant difference in control with status epilepticus, (28.53?10.95) min and (33.85?11.53) min, P

Objective To produce a new type of medical bed sheet suitable for hemodialysis .Methods Application effect of traditional methods compared hemostatic clamp fixed cycle trail tube with the use of dialysis sheets fixed cycle trail tube method in hemodialysis .Results Dialysis sheets of reasonable structure setting has the advantages of convenience and practicality than using sterilized forceps traditional fixed mode .The adaptability is strong, and preparation cost is low .The fixing device and the sheets are fixed as a whole , without also prepare disin-fection clamp fixed cycle trail tube , not circulating blood road pipe clamp bad , and put an end to the sheets caused by repeated clamping forceps to damage , and can be used as a piece of goods one -time cleaning and disinfection .Con-clusion The standard of energy saving and consumption reduction is achieved by dialysis bed sheets , which can a-chieve the goal of controlling and improving the quality of medical care .

Objective:To investigate the prehension status of outpatients for oral health care knowledge.Methods:Refering to scheme of oral health propaganda in West-Pacific area instituted by World Health Organization,self-designed questionnaire was used and 478 outpatients with diferent education levels were interviewed about the prehension for oral health care knowledge. The results were analyzed.Result:There was no difference among patients with different sexes in basic oral health care knowl- edge,but with regard to the question that there is association between cigarette and periodontal diseases,the prehension status of female patients was better than that of the male patients.Meanwhile,The prehension status of the patients with university e- ducation was better than that of the patients without university education,but generally,the correct rate was not high.Conclu- sion:The education level will affect the prehension status.The prehension status of outpatients for oral health care knowledge is not ideal.We must emphasize the importance of the oral health care and the oral health care knowledge education.

Objective To investigate the effect of molecular chaperone on the prokaryotic expression level and activity of tetanus toxin fragment C(TTC) in order to provide new ideas for the development of new tetanus vaccines.Methods The gene sequence of TTC was optimized for E.coli adaptive codons,and the recombinant plasmid pET30a-HC was constructed,which was identified by double enzyme digestion.The recombinant plasmid pET30a-HC and molecular chaperone pGro7were co-transformed into competent E.coli BL21(DE3) and induced by IPTG to express TTC.The obtained TTC was identified by 12% SDS-PAGE and Western blot,and purified by Ni FF metal chelate affinity chromatography column.Female Kunming mice were immunized with TTC,TTC+Al,TTC+CpG,PBS,and tetanus toxoid(TT) by subcutaneous injection,10 mice in each group.After immunization,blood samples were collected by tail cutting or eyeball picking,and the serum was separated.The antibody titer,antibody typing,IL-4 and IFNγ levels were detected by ELISA.The lung and liver of mice were harvested aseptically,and the safety of TTC was evaluated by histopathological method.Results The recombinant plasmid pET30a-HC was constructed correctly as identified by double enzyme digestion.After adding molecular chaperone pGro7,the soluble expression of the target protein accounted for 40% of the total supernatant expression.After 42 days of the initial immunization,the specific IgG antibody titer in serum of TTC+CpG group was significantly higher than that of PBS,TTC,and TT groups(t=4.753-8.624,each P <0.01),which was also higher than that of TTC+Al group,but there was no significant difference(t=0.966,P> 0.05).There was no significant difference in serum IgG1,IgG2a,IgG2b,IgG3,and IgGM antibody levels among the groups(t=0.163-0.203,P> 0.05),IgG1/IgG2a> 1,indicating that the immune pathway was biased towards Th2 type.Compared with TTC group,the serum IL-4 and IFNγ levels in TTC+Al group had no significant difference(t=2.666 and 2.470,respectively,each P> 0.05),while significantly increased in TTC+CpG group(t=18.080 and8.440,P <0.001 and <0.05,respectively).Compared with TT group,the serum IL-4 level in TTC+CpG group increased significantly(t=8.561,P <0.01),but there was no significant difference in IFNγ level(t=0.472,P> 0.05).No obvious pathological changes were found in lung and liver of mice in each group.Conclusion Molecular chaperone can improve the soluble expression level of TTC with good safety,and combined with CpG adjuvant,it can induce strong immune response.

Objective To compare the effectiveness of the three operation mode in treatment of ovarian cyst. Methods According to the digital table,138 patients with ovarian cyst were divided into three groups,the group A (n=46 cases)were operated through open operation,group B (n =46 cases)were operated through transvaginal operation,group C(n=46 cases)were operated through laparoscopic operation.The three groups were observed the situation of operation (operation time,bleeding volume,the first exhaust time,hospitalizationtime ),complications (infection,hemorrhage,perimenopausal changes ),postoperative follow -up for 1 years.The recurrence rate was observed.Results Group A of 46 patients were successfully completed surgery,B,C groups of 1 patients was con-versed to laparotomy due to severe adhesion.B,C groups in the operation time,amount of bleeding during the opera-tion,the time of hospitalization were significantly less than that of group A (t=4.306,5.172,3.012,3.926,3.776, 2.168,P<0.05,P<0.01).B group was significantly less than those of A,C group in the first exhaust time (t=3.014,2.446,all P<0.05).In the operation time of group B was obviously less than that of group C (t=2.748,P<0.05).A,B,C three groups of postoperative complication rates were 39.1%,11.1%,15.6% respectively;the incidence of complications of B,C groups was lower than that in A group (χ2 =9.82,8.64,all P<0.01 ).After 1years follow-up,the recurrence rate in group B was significantly higher than that of A and C groups (χ2 =6.72, 6.72,all P<0.01).Conclusion Treatment of ovarian cyst vaginal surgery and laparoscopic surgery wound is small, but the person that weigh should be performed open adhesion treatment,individualized treatment.

Objective To investigate the effect of acupuncture at points Taiyang and Fengchi on the expression of heat shock protein 70 (HSP70) and neural cell apoptosis during local cerebral ischemia and reperfusion in rabbits and explore its protective action on nerve cells and the mechanism.Methods Rabbits were randomized into sham operation, model and acupuncture groups. Each group was randomly allocated to three time points: one, three and seven days. A rabbit model of local cerebral ischemia and reperfusion was made by middle cerebral artery occlusion (MCAO)using an intraluminal thread. Reperfusion was performed at two hours after cerebral ischemia. In the acupuncture groups, points Taiyang and Fengchi were electro-acupunctured at six hours after cerebral ischemia. Treatment was given once daily. The rabbits were sacrificed to take materials at one, three and seven days after reperfusion. Pathological changes in brain tissue were observed by HE staining. Cerebral HSP70 expression was determined by immunohistochemical method. Neural cell apoptosis was examined by TdT-mediated dUTP nick end labeling (TUNEL).Results Pathological staining showed that cerebral infarction area decreased significantly, the number of nerve cells increased and inflammatory infiltration reduced at every time point in the acupuncture group of rabbits compared with the model group. The results of histochemical examination showed that HSP70 expression decreased in the sham operation group and increased in the model group. It increased significantly at every corresponding time point in the acupuncture group compared with the model group (P<0.01). The number of apoptotic cells increased gradually with reperfusion prolongation and reached the peak at three hours in the model group. The number of apoptotic cells decreased significantly at every time point in the acupuncture group and there was a statistically significant difference by comparison with the model group (P<0.01).Conclusions Electroacupuncture at points Taiyang and Fengchi can increase cerebral HSP70 expression and decrease neural cell apoptosis to produce a nerve-protecting effect in rabbits with cerebral ischemia-reperfusion injury.

Objective:To design real-time transmission system for pre-hospital emergency electrocardiogram(ECG) so as to provide specialty and accurate diagnostic analysis about ECG before patient achieved at hospital and to shorten the waiting time for diagnosis and treatment after they achieved at hospital.Methods: 4G network and virtual private dial-up network(VPDN) were used to real-time transmit ECG data of patient from ambulance to hospital so as to doctors of ECG department and emergency room can analyze the detecting reporter, choose plan of emergency guidance and prepare operation and treatment for patientsaccording to the received ECG data in time.Results: The situations of emergency patients have been grasped and been primarily confirmed before they achieved hospital, therefore, the doctors could made relative preparation for fast and specially treating patients after they once achieved hospital.Conclusion: The real-time transmission system of pre-hospital emergency ECG can remedy the shortage of technical strength of pre-hospital emergency team, and shorten the treating time of emergency patients, and increase the success rate of rescue.

Fu biao who got an advanced liver cancer had undergone liver-transplantation twice and died at last,which raises great ethical concern.With respect to distribution,there are four substantial distributive principles,including the principle of equality,the principle of need,the principle of contribution,the principle of effort.When allocating scarce health-care resouses,medical criteirion should be considered firstly the equality of everyone's life.Furthermore,the principle of utility and equality should be embodied for ensuring distributive justice.