AIM: A methond was established for the determination of emodin and chrysophanol in Zhenhuang Powder (Margarita, Radix et Rhizoma Rhei, artificial Calculus Bovis, etc.). METHODS: RP HPLC was used for samples separated on a shim pack VP ODS column with the mobile phase of methanol-0.1% H 3PO 4(85∶15) and the detection wavelength was set at 430nm. RESULTS: The calibration curves were linear in the range of 0.01181～4.724?g( r =0.9994) for emodin and in the range of 0.01300～5.200?g( r =0.9999) for chrysophanol, respectively. The average recoveries were 99.0%( RSD =1.23%, n =5) and 100.5% ( RSD =1.06%, n =5), respectively. CONCLUSION: The method is simple, realizable and reproducible. It can be used effectively for the quality control of Zhenhuang Powder.

Objective:To observe the effects of Chinese herb baicalin on proliferation and cell cycle of periodontal ligament cells(PDLC) by stimulation of lipopolysaccharide(LPS) in vitro.Methods:Human PDLC was cultured in vitro.MTT colormetic method was used to evaluate the effects of Chinese herb baicalin on proliferation of PDLC by stimulation of LPS.Flow cytometry was used to analyze the possible DNA index and cellular cycle changes of PDLC.Results:PDLC showed a significant growth inhibition after being treaded with 100 ?g/ml LPS 3 h by MTT methods,and proliferation ability of PDLC improved greatly after adding baicalin.FCM results showed that the cell number increased in G1 phase,while the cell number decreased in S phase at 12 h after treated with 100 ?g/ml LPS,but the cell number decreased in G1 phase,and increased in S phase after treated with Chinese baicalin.Conclusion:Chinese herb baicalin can significantly promote the proliferation of PDLC.It can make some static cell in G1 phase enter S phrase and improve the growth of human PDLC.

Objective:To establish a detection method for residual toluene in diclofenac sodium raw material by GC. Methods:An HP-5 column (0. 53 mm × 30 m, 2. 65 μm) with nitrogen as the carrier gas was employed for the separation. The temperature of the column, injection port and FID detector was set at 50℃, 200℃ and 250℃, respectively. An internal standard method with isooctane as the internal standard was used to assay the residual amount after the sample was dissolved in DMSO. Results:The linear calibration curve was obtained over the range of 9. 27-111. 19μg·ml-1(r=0. 999 4). The mean recovery was 97. 27%(RSD=2. 80%,n=9). The LOD and LOQ was 0. 59μg·ml-1 and 1. 46μg·ml-1 , respectively. The residual contents of toluene in 3 batches of the products all met the relevant regulations. Conclusion:The established method is reliable and accurate, and can be used in the quality control of diclofenac sodium.

Combined with the partial least square regression, the oc tane number (RON), density, temperature evaporate (50%,90%), aromatics and olefi n concentration of gasoline were determined by the Fourier transform infrared spe ctroscopy at the wavenumber region of 1550～660cm－1. The experimenta l results showed that the actual values measured by the standard method were agre e with the prediction values by the proposed method.

Objective To describe uncertainty in illness of patients with hepatocellular carcinoma intending to undergo transcatheter artery chemoembolization (TACE) and to identify the related factors. Methods A descriptive and correlative design was used. By convenience sampling, 95 patients with hepatocellular carcinoma were recruited from Sun Yat-Sen University Cancer Center in this study. Mishel Uncertainty in Illness Scale was used to assess uncertainty in illness and information needs of patients with hepatocellular carcinoma intending to undergo TACE and the demographic questionnaire and Information Needs Assessment Scale were used to measure demographic data, disease and treatment characteristics and information needs of patients. Results The level of uncertainty in illness in most patients with hepatocellular carcinoma intending to undergo TACE was middle (77.61±9.15) points. The mean score of indeterminacy subscale was (50.16 ± 6.16) points. Uncertainty in illness of patients with hepatocellular carcinoma intending to undergo TACE was affected by the degree of education background, domicile, family economic status, way to pay for the medical expenses, course of diseases, how many times he (she) used to undergo assisted treatments and the level of information needs. Conclusions The level of uncertainty in illness in most patients with hepatocellular carcinoma intending to undergo TACE was middle and it is important to assess patients′uncertainty in illness at clinical work and to take effective interventions to content patients′information needs to decrease the level of uncertainty.

Objective To study the antitumor activities and immunity regulation of Hedyotis diffusa Willd injection. Methods Inhibitory effects of Hedyotis diffusa Willd injection on cell proliferation was detected with MTT method. Then the inhibitive rate was calculated. The immunomdulatory effects of Hedyotis diffusa Willd injection were investigated. Results Hedyotis diffusa Willd injection inhibited the proliferation of A549, SGC-7901, HEP-G2, Helacells in the dose of 100～20 ?g/mL, and enhance the immunity regulation on S180 tumor transplanted in mice. Conclusion Hedyotis diffusa Willd injection has the antitumor activities and can enhance the immunity.

OBJECTIVE:To establish a method for preparation and quality control of compound dexamethasone liniment.METHODS:The compound dexamethasone liniment were prepared with dexamethasone acetate and vitamin E,the contents were determined by HPLC.RESULTS:The calibration curves of dexamethasone acetate and vitamin E were linear in the range of2～40?g/ml and80～800?g/ml respectively,and their average recoveries were100.5%(RSD=0.82%)and98.6%(RSD=0.59%)respectively.CONCLUSION:The preparation is reasonable in formula,simple and accurate in quality control.

Objective To assess the relationship between the HLA gene sharing of couples and gestational diabetes mellitus (GDM). Methods Thirty GDM women and their husbands served as study group, 40 normal pregnant women and their husbands were selected as control group. All pregnant women were primipara with single fetus. 5 ml cubital venous blood were taken. Genomic DNA was extracted using phenol chloroform method. HLA-DRB alleles type were determined with PCR-SSP. Results The sharing of HLA-DRB1 between couples in GDM group were 33.33%, significantly higher than that of normal pregnancy(12.50%)(P0.05). Conclusions The sharing of HLA-DRB1 between couples may inerease the risk of GDM, It suggested that placenta- and-fetus unit may play roles in GDM.

Objective To measure the erythrocyte dielectric response variation of patients with KaschinBeck disease,to explore the pathogenesis of Kaschin-Beck disease and seek more effective means for evaluating the effectiveness of early diagnosis and control measures against Kaschin-Beck disease.Methods According to the principle of dielectrophoresis and cell dielectric response phenomena,dielectrophoretic pool was manufactured and dielectrophoretic detection and separation system was established.Patients with Kaschin-Beck disease (438) were selected in Kaschin-Beck disease areas of Aba County,Sichuan.At the same time,healthy persons (480) in KaschinBeck disease areas and non-Kaschin-Beck disease areas in Aba County were selected.Anticoagulant blood samples from patients with Kaschin-Beck disease and healthy persons were collected,erythrocytes were separated,cell suspension (10s cells/ml) was prepared and 200 μl cell suspension was added to the dielectrophoretic pool to measure erythrocyte dielectric response rate.Results Erythrocyte dielectric response rates were significantly different between patients living in Kaschin-Beck disease areas with Kaschin-Beck disease [(75.87 ± 5.89)％] and healthy persons living in Kaschin-Beck disease areas and non-Kaschin-Beck disease areas [(92.43 ± 4.45)％,(92.81 ±5.01)％,F =1.843,P ＜ 0.01).Erythrocyte dielectric response rate was significantly reduced in patients with Kaschin-Beck disease compared with that of healthy persons (all P ＜ 0.01);erythrocyte dielectric response rate of adult patients with Kaschin-Beck disease was lower [(69.57 ± 6.87)％] than that of pediatric patients with Kaschin-Beck disease [(82.17 ± 4.91)％,P ＜ 0.01].Erythrocyte dielectric response rates were significantly different in patients with different degrees of Kaschin-Beck disease (F =1.647,P ＜ 0.01).Erythrocyte dielectric response rate of patients with Kaschin-Beck disease was negatively correlated with prevalence of the disease (r =-0.87,P ＜ 0.01).Conclusions Erythrocytes of patients with Kaschin-Beck disease have some degree of pathological damage.The more severe the disease,the more serious the damage of red blood cell.The change of erythrocyte dielectric response properties may be used as an index to judge the prevalence of Kaschin-Beck disease and for early diagnosis.

Objective To investigate the influence of health care workers’(HCWs)hand hygiene intervention in healthcare-associated infection(HAI)in an intensive care unit(ICU).Methods Intervention measures of hand hygiene were adopted,effective supervision and management systems were established,hand hygiene compliance and HAI rate be-fore intervention(January-December 2012)and after intervention (the first stage:January-June 2013;the second stage:Ju-ly-December 2013)were compared.Results A total of 4 066 patients were surveyed,HCWs’hand hygiene compliance rate before intervention was 50.03%,the first and second stage after intervention was 61.80% and 64.57% respectively,there was increasing trend (rs=1.00,P<0.001).HAI before intervention was 5.48%,the first and second stage after inter-vention was 3.86% and 3.30% respectively,there was decreasing trend (rs=-1.00,P<0.001).Significant decreasing trends were found in rates of catheter-related bloodstream infection(CRBSI),catheter-associated urinary tract infection (CAUTI)and ventilator-associated pneumonia(VAP)(rs=-1.00,P<0.001).There were negative correlations between hand hygiene compliance rate and the infection rate of ICU,infection rate of CRBSI,CAUTI and VAP (P<0.05 ). Conclusion The improvement of hand hygiene compliance can effectively reduce the incidence of HAI in ICU ward.