Objective:To establish a detection method for residual toluene in diclofenac sodium raw material by GC. Methods:An HP-5 column (0. 53 mm × 30 m, 2. 65 μm) with nitrogen as the carrier gas was employed for the separation. The temperature of the column, injection port and FID detector was set at 50℃, 200℃ and 250℃, respectively. An internal standard method with isooctane as the internal standard was used to assay the residual amount after the sample was dissolved in DMSO. Results:The linear calibration curve was obtained over the range of 9. 27-111. 19μg·ml-1(r=0. 999 4). The mean recovery was 97. 27%(RSD=2. 80%,n=9). The LOD and LOQ was 0. 59μg·ml-1 and 1. 46μg·ml-1 , respectively. The residual contents of toluene in 3 batches of the products all met the relevant regulations. Conclusion:The established method is reliable and accurate, and can be used in the quality control of diclofenac sodium.

AIM: A methond was established for the determination of emodin and chrysophanol in Zhenhuang Powder (Margarita, Radix et Rhizoma Rhei, artificial Calculus Bovis, etc.). METHODS: RP HPLC was used for samples separated on a shim pack VP ODS column with the mobile phase of methanol-0.1% H 3PO 4(85∶15) and the detection wavelength was set at 430nm. RESULTS: The calibration curves were linear in the range of 0.01181～4.724?g( r =0.9994) for emodin and in the range of 0.01300～5.200?g( r =0.9999) for chrysophanol, respectively. The average recoveries were 99.0%( RSD =1.23%, n =5) and 100.5% ( RSD =1.06%, n =5), respectively. CONCLUSION: The method is simple, realizable and reproducible. It can be used effectively for the quality control of Zhenhuang Powder.

Objective:To observe the effects of Chinese herb baicalin on proliferation and cell cycle of periodontal ligament cells(PDLC) by stimulation of lipopolysaccharide(LPS) in vitro.Methods:Human PDLC was cultured in vitro.MTT colormetic method was used to evaluate the effects of Chinese herb baicalin on proliferation of PDLC by stimulation of LPS.Flow cytometry was used to analyze the possible DNA index and cellular cycle changes of PDLC.Results:PDLC showed a significant growth inhibition after being treaded with 100 ?g/ml LPS 3 h by MTT methods,and proliferation ability of PDLC improved greatly after adding baicalin.FCM results showed that the cell number increased in G1 phase,while the cell number decreased in S phase at 12 h after treated with 100 ?g/ml LPS,but the cell number decreased in G1 phase,and increased in S phase after treated with Chinese baicalin.Conclusion:Chinese herb baicalin can significantly promote the proliferation of PDLC.It can make some static cell in G1 phase enter S phrase and improve the growth of human PDLC.

Objective To describe uncertainty in illness of patients with hepatocellular carcinoma intending to undergo transcatheter artery chemoembolization (TACE) and to identify the related factors. Methods A descriptive and correlative design was used. By convenience sampling, 95 patients with hepatocellular carcinoma were recruited from Sun Yat-Sen University Cancer Center in this study. Mishel Uncertainty in Illness Scale was used to assess uncertainty in illness and information needs of patients with hepatocellular carcinoma intending to undergo TACE and the demographic questionnaire and Information Needs Assessment Scale were used to measure demographic data, disease and treatment characteristics and information needs of patients. Results The level of uncertainty in illness in most patients with hepatocellular carcinoma intending to undergo TACE was middle (77.61±9.15) points. The mean score of indeterminacy subscale was (50.16 ± 6.16) points. Uncertainty in illness of patients with hepatocellular carcinoma intending to undergo TACE was affected by the degree of education background, domicile, family economic status, way to pay for the medical expenses, course of diseases, how many times he (she) used to undergo assisted treatments and the level of information needs. Conclusions The level of uncertainty in illness in most patients with hepatocellular carcinoma intending to undergo TACE was middle and it is important to assess patients′uncertainty in illness at clinical work and to take effective interventions to content patients′information needs to decrease the level of uncertainty.

Objective To study the antitumor activities and immunity regulation of Hedyotis diffusa Willd injection. Methods Inhibitory effects of Hedyotis diffusa Willd injection on cell proliferation was detected with MTT method. Then the inhibitive rate was calculated. The immunomdulatory effects of Hedyotis diffusa Willd injection were investigated. Results Hedyotis diffusa Willd injection inhibited the proliferation of A549, SGC-7901, HEP-G2, Helacells in the dose of 100～20 ?g/mL, and enhance the immunity regulation on S180 tumor transplanted in mice. Conclusion Hedyotis diffusa Willd injection has the antitumor activities and can enhance the immunity.

OBJECTIVE:To establish a method for preparation and quality control of compound dexamethasone liniment.METHODS:The compound dexamethasone liniment were prepared with dexamethasone acetate and vitamin E,the contents were determined by HPLC.RESULTS:The calibration curves of dexamethasone acetate and vitamin E were linear in the range of2～40?g/ml and80～800?g/ml respectively,and their average recoveries were100.5%(RSD=0.82%)and98.6%(RSD=0.59%)respectively.CONCLUSION:The preparation is reasonable in formula,simple and accurate in quality control.

Combined with the partial least square regression, the oc tane number (RON), density, temperature evaporate (50%,90%), aromatics and olefi n concentration of gasoline were determined by the Fourier transform infrared spe ctroscopy at the wavenumber region of 1550～660cm－1. The experimenta l results showed that the actual values measured by the standard method were agre e with the prediction values by the proposed method.

Objective: Adopting quantified index to observe and analyze the effect of acute and chronic pain through double-wavelength diode laser. Methods: Using diode laser with the wavelength of 650nm / 810nm to irradiate the special tissues or aching points of patient, selecting different frequency, intensity and time to make treatment. Results: VAS scoring: P<0.05, the total efficient rate is 96%. Conclusions: Treatment with double-wavelength diode laser to various acute and chronic pain is just a definitely good curative effect, and it can release the effects of patient sleeping, daily life, communication with people and life interests caused by pain, and finally enhancing the quality of life.

Objective:To efficiently amplify NK cells and determine their cytotoxic activity against a variety of tumor cell lines in vitro,thereby providing evidence for potential clinical application.Methods: PBMCs were isolated from adult peripheral blood and co-cultured with K562 cells that were genetically modified to express 4-1BBL,IL-15 and IL-21 on the surface for 15 days to effectively amplify NK cells.The total cell number and Purity of CD3-CD56+ cells were measured.Granzyme B and perforin expression of the amplified NK cells were detected by flow cytometry and real-time PCR.The anti-tumor effect on different cancer cells was evaluated.Results: This method obtained a more than 1.1×1010 CD3-CD56+ NK cells with 95% purity over a 15 day amplification procedure.The expanded NK cells could efficiently release granzyme B and Perforin.The cytotoxicity against different tumor cells was followed the order from strong to weak:gastric,pancreatic,cervical,ovarian and renal cancer cells,with the highest activity against gastric cancer cell line A549 (90% at E∶T=10∶1) (P<0.05).A time-dependent killing effect of activated NK cells on cervical,liver and pancreatic cancer cells was observed.Conclusion: This amplification procedure can consistently generate large amounts of pure NK cells with effective cytotoxic function against a variety of tumor cells.

Objective To study the risk factors for catheter-related bloodstream infection (CRBSI)in an intensive care unit (ICU),and provide scientific evidence for CRBSI prevention and control.Methods 1 677 ICU patients with central venous catheterization (CVC)for>48 hours between January 2008 and December 2012 were divided in-to CRBSI group and non-CRBSI group,risk factors for CRBSI were analyzed.Results The utilization rate of CVC was 92.88% (21 041 d);86 (5.13%)patients developed CRBSI,the incidence of CRBSI per 1 000 catheterization-day was 4.02,the mortality of CRBSI group was significantly higher than non-CRBSI group (58.14% [50/86]vs 36.83%[586/1 591])(χ2 =15.74,P <0.01 ).Multivariate logistic regression analysis showed that the risk factors for CRBSI in-cluded length of stay in ICU>5 days,CVC>5 days,the episode of CVC>1 (P <0.01).Conclusion Realizing the occur-rence status and risk factors of CRBSI in ICU patients can provide reference for further targeted monitor and implementation of zero tolerance goal of the CRBSI.