Objective To increase the recognition of the clinicians and laboratorians to hereditary spherocytosis for reducing the misdiagnosis and missed diagnosis ,and improving the diagnostic level .Methods The data in 4 cases of definitely diagnosed heredi‐tary spherocytosis in our hospital were retrospectively analyzed ,and the misdiagnosis reasons were analyzed and summarized .Results In 4 cases ,2 cases were misdiagnosed as Mediterranean anemia ,1 case was misdiagnosed as hemolytic anemia and 1 case as hemo‐globin disease .Conclusion Correctly mastering the key points of the diagnosis ,strengthening the communication between the labo‐ratorians and clinicians ,paying attention to the red blood cells morphologic examination under microscope and high level diagnosis a‐bility of blood cell morphology are the important means to reduce the misdiagnosis and missed diagnosis and improve the diagnostic accuracy rate .

Objective To investigate the change of peripheral blood lymphocyte immunophenotype in the patients with malignant carcinoma and its clinical significance .Methods The flow cytometry was adopted to detect the expression levels of peripheral blood T lymphocyte subpopulations ,CD19+ cells ,CD3-CD56+ cells ,CD3+CD56+ cells in 167 patients with malignant carcinoma (inclu‐ding respiratory tract cancers ,gastrointestinal cancers and reproductive system cancer) and 170 normal controls as the healthy con‐trol group .The contrastive analysis was performed .Furthermore the analysis was conducted according to whether having tumor me‐tastasis and chemotherapeutic cycles .Results The total absolute value of the lymphocytes and the proportion of CD3+CD4+ cells in the patients with malignant carcinoma were significantly lower than those in the healthy control group (P<0 .05) ,but the propor‐tions of CD3+CD8+ ,CD3+CD56+ and CD3-CD56+ cells were significantly higher than those in the healthy control group (P<0 .05) .The proportion of CD3+CD4+ cells in the patients with tumor metastasis were significantly lower than those in the ones without tumor metastasis ;but the proportions of CD3+ CD8+ ,CD3-CD56+ and CD3+ CD56+ cells were significantly higher than those in the ones without tumor metastasis ,the differences were statistically significant(P<0 .05) .In the chemotherapy treatment , the proportions of CD3+CD4+ ,CD3+CD56+ and CD3-CD56+ cells were gradually increased with the increase of chemotherapeutic cycles ,but the proportion of CD3+CD8+ cells was gradually reduced .Conclusion In the process of development ,progression and treatment of tumor ,the cellular immunity function plays a dominant role ,which could be used as one of indexes for clinically moni‐toring the cellular immune function in the patients with tumor .