Objective To investigate the expression of forkhead box C2 (FOXC2),Vimentin and E-cadherin protein in colorectal adenocarcinoma and the relationship of FOXC2,Vimentin and E-cadherin with the clinicopathological parameters,and the role of epithelial-mesenchymal transition (EMT) in colorec tal adenocarcinoma and the correlation between FOXC2 and EMT.Methods The expressions of FOXC2,Vimentin and E-cadherin in 102 cases of colorectal adenocarcinoma tissues and 30 cases of adjacent noncancerous tissues were detected by immunohistochemical method,and the correlation between FOXC2 and EMT and the clinicopathological parameters of colorectal adenocarcinoma were analyzed.Results The positive rates of FOXC2,Vimentin and E-cadherin in colorectal adenocarcinoma tissues were 53.9％,40.2％ and 26.5％,respectively.The positive rates of FOXC2,Vimentin and E-cadherin in adjacent noncancerous tissues were 6.7％,0 and 63.3％.Compared to paracancerous tissue,the expression of FOXC2 and Vimentin in colorectal adenocarcinoma was significantly increased,while the expression of E-cadherin was significantly decreased.The positive rates of FOXC2 and Vimentin in lymph node metastasis group were 79.1％ and 67.4％,respectively,which were significantly higher than those without lymph node metastasis (35.6％,P ＜0.01;20.3％,P ＜0.01).The positive rate of E-cadherin in lymph node metastasis group was 16.3％,which was significantly lower than that without lymph node metastasis (33.9％,P ＜ 0.01).The positive rates of FOXC2 and Vimentin in T1 +T2 phase were 41.7％ and 25.0％,respectively,which were significantly lower than those in T3 + T4 stage (83.3％,P ＜0.01;76.7％,P ＜0.01).The positive rate of Ecadherin in T1 + T2 stage group was 33.3％,which was significantly higher than that in T3 + T4 stage (10.0％,P ＜ 0.01).There was a positive correlation between FOXC2 and Vimentin in colorectal adenocarcinoma (P ＜0.01),and negatively correlated with E-cadherin expression (P ＜ 0.01).Conclusions EMT may promote the development and metastasis of colorectal adenocarcinoma,FOXC2 may be involved in colorectal cancer EMT and through the EMT to promote the malignant process of colorectal adenocarcinoma.

0 05). For the average blood loss at 2 hour postpartum, it was 129 7 ml, 133 9 ml, 168 5 ml and 178 2 ml for group Ⅰ, Ⅱ, Ⅲ and Ⅳ respectively, while the total blood loss for the 4 groups was 243 3 ml, 242 9 ml, 308 1 ml, and 314 8 ml respectively. The average blood loss of group Ⅰ and Ⅱ was significantly less than group Ⅲ and Ⅳ ( P 0 05). The occurrences of postpartum hemorrhage (blood lose ≥400 ml) were 6 4%, 13 3%, 20 7% and 25 3% for group Ⅰ, Ⅱ, Ⅲ and Ⅳ respectively. There was no major adverse effects appeared. Conclusions Transamin is efficient and safe in reducing the postpartum blood loss. 1 0 g of Transamin has the best efficacy, and 0.5g of Transamin followed.

Objective To investigate the gene polymorphism of inhibitory killer cell immunoglobulin- like receptor (iKIR) in patients with inflammatory bowel disease (IBD) and whether the iKIR gene polymorphisms were associated with IBD.Methods Peripheral blood DNA samples were isolated from 100 patients with ulcerative colitis (UC),52 patients with Crohn's disease (CD) and 106 randomly ethnically matched healthy controls.The iKIR gene polymorphisms were analyzed by sequence specific primer polymerase chain reproduction (PCR-SSP).Phenotypic frequency and gene frequency of iKIR gene were calculated,and differences were compared between IBD patients and healthy controls.Results iKIR genes (including KIR2DL1,KIR2DL2,KIR2DL3,KIR2DL4,KIR2DL5,KIR3DL1,KIR3DL2, KIR3DL3) were found to be present in all subjects at different levels.Interestingly,phenotypic frequencies of KIR2DL1 and KIR2DL3 were significantly lower in UC patients than those in healthy controls (P = 0.001),while phenotypic frequencies of KIR2DL2,KIR2DL4,KIR2DL5,KIR3DL1, KIR3DL2 and KIR3DL3 were no difference between UC patients and healthy controls (P>0.05).The phenotype frequency of KIR2DL1 was significantly decreased in CD patients compared with healthy controls (P = 0.007),while phenotypic frequencies of other iKIR were observed to be no significant change between CD patients and healthy controls (P>0.05 ).Conclusions The KIR2DL1 and KIR2DL3 gene phenotype frequencies are decreased in UC patients,which suggests that these gene polymorphisms are associated with the susceptibility of UC,and the polymorphism of KIR2DL1 gene is involved in the susceptibility of CD.

Objective To explore the relationship between maternal pre-pregnancy body mass index (BMI) and neonatal birth weight in women with gestational diabetes mellitus (GDM). Methods From the pregnant women who received prenatal care and delivered at the Department of Obstetrics and Gynecology of Peking University First Hospital between May 1, 2012 and November 1, 2013, 550 GDM women aged 20-49 years and with single gestation were enrolled in this study. According to the pre-pregnancy BMI, the GDM women were divided into overweight group (BMI ≥ 24.0, n=145) and non-overweight group (BMI < 24.0, n=405). Gestational weight gain, glucose level of 75 g oral glucose tolerance test, glucose control, delivery mode and neonatal birth weight were compared between the two groups. The influencing factors for macrosomia and the relationship between maternal pre-pregnancy BMI and neonatal birth weight were analyzed. Independent sample t-test, Chi-square test, multivariate Logistic regression and Pearson correlation analysis were used for statistical analysis. Receiver operating characteristic (ROC) curve was used to determine the optimal threshold of pre-pregnancy BMI to predict macrosomia. Results Compared with the data of non-overweight group, fasting glucose level [(5.1±0.5) vs (5.3±0.5) mmol/L, t=-4.599], 1 h glucose level [(9.4±1.7) vs (9.8±1.6) mmol/L, t= - 2.742], proportion of poor glucose control [20.5% (83/405) vs 33.1% (48/145), χ2=8.281], proportion of cesarean delivery [37.8% (153/405) vs 55.2% (80/145), χ2=13.160], neonatal birth weight [(3 306±424) vs (3 476±545) g, t=-3.374], and ratio of macrosomia [5.4% (22/405) vs 16.6% (24/145), χ2=16.291] were all higher in overweight group (all P < 0.01). The mean gestational weight gain per week in overweight group was significantly lower than in non-overweight group [(336±123) vs (402±131) g, t=5.136, P < 0.01]. Pre-pregnancy overweight (OR=4.009, 95%CI:2.039-7.881), gestational weight gain per week (OR=1.003, 95%CI:1.001-1.005) and fasting glucose level (OR=2.285, 95%CI: 1.326-3.938) were the influencing factors for macrosomia (all P < 0.01). Pre-pregnancy BMI of GDM women was positively related with neonatal birth weight (r=0.179, P < 0.01). Pre-pregnancy BMI ≥ 22.8 was defined as the optimal threshold to predict macrosomia (ROC area under curve=0.691). Conclusions Maternal pre-pregnancy overweight is a significant risk factor for macrosomia in women with GDM. GDM women with pre-pregnancy overweight should control glucose level and weight gain during pregnancy in order to reduce the risk of macrosomia.

Objective To investigate the expression and diagnostic significance of CK19,CD56 and p53 protein in papillary thyroid microcarcinoma and thyroid papillary hyperplasia.Methods The expressions of CK19、CD56 and p53 protein were detected in 52 cases of papillary thyroid microcarcinoma and 31 of thyroid papillary hyperplasia by iImmunohistochemical methods.Results The positive rate of CK19 expression was 100 ％ (52/52) of papillary thyroid microcarcinoma and 29.0 ％ (9/31) in 31 of thyroid papillary hyperplasia.There was significant difference between two groups (P ＜ 0.001).CK56 in 2 cases (3.8 ％) of papillary thyroid microcarcinoma appeared mild positive expression,and in 20 cases (66.7 ％) of thyroid papillary hyperplasia positive expression (P ＜ 0.001).The positive expression rates of p53 were 69.2 ％(36/52) in papillary thyroid microcarcinoma and 6.5 ％ (2/31) in thyroid papillary hyperplasia (P ＜ 0.001).Conclusion CK19,CD56 and p53 may be important value on differential diagnosis of papillary thyroid microcarcinoma from thyroid papillary hyperplasia,and they are the indispensable markers of differential diagnosis.

Objective To investigate the expression and significance of cellular inhibitor of protein phosphatase 2A (CIP2A), bcl-2 and p63 in papillary thyroid cancer (PTC). Methods Using immunohistochemistry to detect the expression of CIP2A, bcl-2 and p63 in 30 cases of nodular goiter (NG), 30 cases of thyroid adenoma (TA) and 57 cases of PTC [including classical PTC (cPTC) 20 cases, papillary microcarcinoma (PMC) 20 cases, follicular thyroid papillary carcinoma (FPTC) 7 cases]. Results In NG group, TA group and PTC group, positive rates of CIP2A were 0, 0 and 94.74 % (54/57), respectively. The differences were statistically significant. In NG group, TA group and PTC group, positive rates of bcl-2 were 16.67 % (5/30), 13.33 % (4/30) and 85.96 % (49/57), respectively. The differences were statistically significant. In each group, positive rates of p63 were 6.67% (2/30), 3.33% (1/30) and 5.26% (3/57), respectively, no significant difference among them. In PTC, expression of CIP2A and bcl-2 were significantly higher than in NG and TA (χ2 = 105.56, P= 0.00; χ2 = 58.95, P= 0.00). Furthermore, the expression of CIP2A and bcl-2 had correlation in PTC (r=0.94, P=0.00). The expression of CIP2A, bcl-2 and p63 had no significantly difference among all the PTC subtype (χ2 values were 2.02, 2.64, 1.85; all P> 0.05). The expression of CIP2A, bcl-2 and p63 was not associated with patients'age, sex, site, lymph node metastasis (all P>0.05). Conclusions High expression of CIP2A and bcl-2 is associated with PTC, and the expression of CIP2A and bcl-2 has correlation in PTC. The expression of p63 has no correlation with PTC.

Objective To investigate the effect of flavopiridol on the proliferation,invasiveness and apoptosis of human prostate cancer cell line LNCaP,and to explore the possibility of its application in clinical treatment.Methods MTT assay was used to detect cell proliferation,cell invasion in vitro was detected by Transwell assay,and flow cytometer was used to observe apoptosis.Results Flavopiridol inhibited the growth of LNCaP cells in a concentration-dependent and time-dependent way (P ＜ 0.05),and reduced the ability of invasion capacity.After treated by 10 nmol/L flavopiridol for 24 h,the apoptosis rate was increased significantly to (7.5±0.9) ％ compared with the control group [(5.3±0.5) ％] (P ＜ 0.05).Conclusion Flavopiridol can inhibit proliferation of LNCaP cells and induce apoptosis,which may be applicable for the treatment of prostate cancer.

Objective To probe into the content of DNA Topo Ⅱ in osteosarcoma after chemotherapy.Methods 30 patients with osteosarcoma received two courses of chemotherapy treatment before the surgical resection of the tumor tissue.Then immunohistochemistry was used to detect the content of Topo Ⅱ in tissues and detected its relationship in pathology.Results There were 8 out of 30 cases in which Topo Ⅱ was presented positive in osteosarcoma (26.7 ％).The protein content of Topo Ⅱ was unrelated to the patient' s age,gender,degree of tumor malignancy,tumor location and translocation or Enneking staging (P ＞ 0.05),but related to patients survival rate (P ＜ 0.05).Conclusion Patients with lower expression of Topo Ⅱ are more likely to have poor prognosis.

Objective To detect the expression of hypoxia-inducible factor 1α (HIF-1α) and bcl-2 ovarian serous carcinoma and their clinical significances. Methods Paraffin specimens including 61 cases of ovarian serous carcinoma and 50 normal ovarian tissues were selected. The expressions of HIF-1α and bcl-2 proteins were detected by immunohistochemical EnVision method and their relationship between them was analyzed. Results The positive rate of HIF-1α and bcl-2 proteins expression in 61 ovarian serous carcinoma was 68.9%and 54.1%, respectively. There was a significant difference between the two groups (χ2=55.381, P< 0.05; χ2= 38.493, P< 0.05). The clinical pathological parameters showed that the positive expression of HIF-1αand bcl-2 proteins were not related with the age (P>0.05). HIF-1αpositive expression was correlated with tumor grades, the state of lymph node metastasis and FIGO stages (χ2=4.931, 25.008, 5.610, P<0.05). Bcl-2 was significantly associated with tumor grades and lymph node metastasis (χ2= 6.956, 33.869, P<0.05), but not with FIGO stages (χ2=3.391, P>0.05). The expression of bcl-2 was positively correlated with HIF-1α in ovarian serous carcinoma (r= 0.304, P= 0.017). Conclusions The expressions of HIF-1α and bcl-2 play a synergic role in the progression of ovarian serous carcinoma. The combined detection of HIF-1αand bcl-2 is effective for patients'prognosis judgment.

Objective To explore the impact of different specific body positions on blood pressure and heart rate.Methods The blood pressure and heart rate of 178 healthy teachers and students was tested with BIOLIGHT M69 in different body positions.Results ①Blood pressure and heart rate were significantly higher in squatting position than in other three specific positions(sitting position, supine position, and standing position) (P<0.01). ②The blood pressure and heart rate were going up from standing position, and standing position with right arm lifting, to standing position with arm weight lifting positions (P<0.01). Conclusion The change of special body positions resulted in change of blood pressure and heart rate.