Objective To assess the effect of ambient air SO2 on mortality of respiratory diseases. Methods Based on the ecological theory, after the adjustment of seasons, temperature, humidity and air pressure, the observed individuals were divided into eight groups. The association between SO2 exposure and mortality rate of respiratory diseases was analyzed using Poisson regression and local auto-regression method. Results SO2 concentration increased by 0.05 mg/m3, the mortality of respiratory diseases increased by 5.90% (95%CI: 2.26%-9.68%) in all groups, in children by 10.23% (95%CI: 2.07%-19.04%), in young-middle-male group by 1.10% (95%CI: -9.51%-12.96%), in young female group by 9.58% (95%CI: -2.57%-23.23%), in young group by 10.23% (95%CI: 2.07%-19.04%), in aged male group by 5.26%(95%CI: 0.01%-10.78%), in aged female group by 8.07% (95%CI: 3.45%-12.89%) and in aged group by 4.66% (95%CI: 1.44%-7.99%) respectively. Conclusion SO2 exposure is found to be associated with the increase of mortality of respiratory diseases, especially in children, aged people and elderly females.

Objective To investigate the nursing intervention and efficacy of docetaxel combined with pirarubicin in the treatment of advanced breast cancer. Methods In our hospital from January 2014 to February 2015 were selected from the patients with advanced breast cancer in 94 cases as the research object, the single and double number (hospital admission order) of the selected patients with advanced breast cancer were randomly divided into study group and control group, 41 cases in each group. 2 groups of patients with advanced breast cancer were treated with docetaxel combined with pirarubicin, the control group was given routine nursing services, and the study group was given comprehensive nursing intervention measures on the basis of routine care. Results Of the 2 groups of patients with advanced breast cancer nursing after different nursing measures the same drug plan, after the study group, the total efficiency of treatment in the method [please explain what is near total efficiency] (82.98%) was significantly better than the control group (61.70%), the difference was statistically significant (P<0.05); the study group the nursing service satisfaction was significantly better than the control group (P<0.05). Conclusion With the combination of docetaxel and pirarubicin in the treatment of advanced breast cancer patients, comprehensive nursing measures can achieve better clinical efficacy and benefit the maintenance of good relationship between nurses and patients.

Objective To estimate the adverse effects of total suspended particles exposure on cardiovascular disease mortality.Methods Cardiovascular disease mortality and TSP monitoring data from1996to2000in Shenyang were collected.The association between TSP concentration and the mortality of cardiovascular diseases was analyzed using Poisson regression model adjusted for season,temperature and humidity variation using a parametric method.Results It was found that each50?g /m 3 increment of TSP was associated with an OR of1.0122(95%CI =1.0036-1.0209)of cardiovascular disease mortality in the general population,an OR of1.0278(95%CI =1.0160-1.0398)in the male group,1.0406(95%CI =1.0294-1.0519)in the female group,1.0430(95%CI =1.0343-1.0519)in the general aged group,1.0577(95%CI =1.0452-1.0703)in the aged male group and1.0427(95%CI =1.0284-1.0572)in the aged female group.Conclusion The results showed that there was an association between TSP concentration and the cardiovascular disease mortality in Shenyang.It was considered that TSP might be one of the risk factors related to the increase of cardiovascular disease mortality.

Objective To assess the advese effects of SO2 on mortality of cardiovascular disease quantitatively. MeThod Based on bionomical theory,after the adjustment of time distribution,temperature,humidity and air pressure,the observed individuals were grouped into total population group,female group,male group,aged group,aged female group and aged male group.The association between SO2 and mortality rate of cardiovascular diseases was analyzed by Poisson regression and local auto-regression method.Ressults A 50?g/m3 increase of SO2 concentration caused 5.33% (95%CI=4.17%～6.49%)increase of mortality rate of cardiovascular disease in total people group,6.96% (95%CI=5.34%～8.60%)in female group,6.43%(95%CI=4.86%～8.03%)in male group,8 14%(95%CI=6 86%～9 46%)in aged group,6 40%(95CI=4 49%～8 34%)in aged female group,9 55%(95%CI=7 74%～11 39%)in aged male gruop.COnclusion SO2 could cause the increase of mortality rate of cardiovascular disease especially ofr aged people with cardiovascular disease.

OBJECTIVE:To study anti-inflammatory,analgesic and anti-fatigue effects of polysaccharides from Acanthopanan trifoliatus (ATMP) in rats and mice. METHODS:In hot plate experiment,150 mice were randomly divided into normal control group (constant volume of normal saline),aspirin group [200 mg/(kg·d)],and ATMP high-dose,medium-dose and low-dose groups [400,200,100 mg(gross polysaccharide)/(kg·d)];the threshold of pain was determined,and analgesic effect of ATMP was investigated. 150 mice were included in exhaustive swimming test and then randomly divided into normal control group(con-stant volume of normal saline),Chongcao yangshen jijing group [400 mg/(kg·d)by total saponins],and ATMP high-dose,medi-um-dose and low-dose groups [200,100,50 mg(gross polysaccharide)/(kg·d)];the body weight and exhaustive swimming time of mice were determined,and biochemical process was used to determine the contents of hepatic glycogen and muscle glycogen, and serum levels of BUN,LDH and CK in mice. The anti-fatigue effect of ATMP was investigated. In carrageenan-induced paw swelling experiment,40 rats were divided into normal control group (constant volume of normal saline),dexamethasone acetate group [5 mg/(kg·d)],ATMP high-dose,medium-dose and low-dose groups [100,50,25 mg(gross polysaccharide)/(kg·d)];the degree of paw swelling was recorded,and anti-inflammatory of ATMP was investigated. RESULTS:Compared with normal control group,the threshold of pain in mice were increased in ATMP 400,200,100 mg(gross polysaccharide)/(kg·d)groups;the exhaus-tive swimming time of mice were prolonged significantly,and the contents of hepatic glycogen in mice were increased significant-ly,while serum contents of CK decreased in ATMP 200,100 mg(gross polysaccharide)/(kg·d)groups;the content of muscle gly-cogen in mice was increased significantly in ATMP 200 mg (gross polysaccharide)/(kg·d) group,while serum contents of BUN and LDH were decreased;the degree of paw swelling in rats was decreased in ATMP 100,50,25 mg(gross polysaccharide)/(kg·d) groups,with statistical significance (P<0.01 or P<0.05). There was no significant difference in body weight of mice before and after medication. CONCLUSIONS:ATMP has significant analgesic and anti-fatigue effects on mice and anti-inflammatory effect on rats.

Objective:To explore the relationship of NSCL/P with MTHFR gene polymorphism in Xinjiang Uyghur and Han popula-tion and the ethnic difference.Methods:rs1801131 and rs1801133 polymorphism was detected by SNaPshot genotype method in 170 children with NSCL/P and 100 healthy controls of Uyghur and Han population.Results:Rs1801133 TT and T allele was statistically difference between 2 nationalities(P <0.05),Rs1801133 CT and CT +TT genotypes in total case group and control group were sta-tistically different(P <0.05);Rs1801131 and rs1801133 conjoint analysis showed that between the 2 nationalities and between case and control groups of total population were statistically different(P <0.05);rs1801131 genotype between 2 nationalities or total cases and controls were not statistically different(P >0.05).Conclusion:Rs1801133 TT and T allele in Han nationality are more likely to suffer from NSCL/P than in Uyghur,rs1801133 CT and CT +TT genotypes are protective factors.Rs1801131AC and rs1801133CC conjoint is relevant to NSCL/P,and the risk in Uyghur is higher than in Han.MTHFR rs1801131 gene polymorphism may not be relat-ed with NSCL/P in Uyghur or Han.

Objective To observe the availability and safety of ventricular septal defect (VSD) occluder in infants and young children with large patent ductus arteriosus (PDA) associated with severe pulmonary hypertension.Methods Five patients (1 male and 4 fomale) of large PDA aged 5 months to 3 years,weighted from 5.1 to 15 kg,body surface area (BSA) 0.37-0.58 m2 underwent transcathter intervention with concentric VSD occluders from June 2008 to May 2009.Arterial ducta were tube-like and their diameters were 5.7 to 8.5 mm,with ulmonary vascular resistance from 4.8 to 5.7 Wood Unit,Qp/Qs 3.4-4.6.Three patients were given Bosentan after intervention.Results The large PDAs were successfully closed with VSD occluders,including 1 concentric perimembranous VSD occluder and 4 muscular VSD occluders.They all discharged 4 to 5 days with hidrosis and weight improved.Echocardiogram indicated VSD occluder was stable,no residue shunt and no stricture of left pulmonary artery and descending aorta were found.According to tricuspid and pulmonary regurgitation,pulmonary arterial pressure decreased differently and returned to normal after 6 months follow-up.Conclusion VSD occluder is available and effective to close large PDA associated with severe pulmonary hypertension in inrants and young children,but more cases and long-term follow-up are necessary.

Objective To develop the clinical practice guidelines for the management of medication adherence to highly active antiretroviral therapy (HAART) in China. Methods The development methods included qualitative interview of 31 stakeholders, questionnaire survey of 423 PLHIV, adaptation of 30 clinical practice guidelines related to AIDS care, and overviews of reviews of 44 systematic reviews/Meta-analysis. Results 10 clinical practice guidelines and 10 systematic reviews/Meta-analysis were included. The clinical practice guidelines for the management of HAART were formed. Conclusions The formed clinical practice guidelines showed better applicability and higher general quality. It is recommended to use the guidelines in AIDS care.

Background and Objectives: Chronic obstructive pulmonary disease (COPD) is a common, frequently-occurring disease and poses a major health concern. Unfortunately, there is current no effective treatment for COPD, particularly emphysema. Recently, experimental treatment of COPD using mesenchymal stem cells (MSCs) mainly focused on bone marrow-derived MSCs (BM-MSCs). Human umbilical cord-derived MSCs (hUC-MSCs) have more advantages compared to BM-MSCs. However, studies on the role of hUC-MSCs in management of COPD are limited. This study sought to explore the role of hUC-MSCs and its action mechanisms in a rat model of VEGF receptor blocker SU5416-injured emphysema. Methods: and Results: hUC-MSCs were characterized by immunophenotype and differentiation analysis. Rats were div-ided into four groups: Control, Control＋MSC, SU5416 and SU5416＋MSC. Rats in model group were administered with SU5416 for three weeks. At the end of the second week after SU5416 administration, model group were infused with 3×106 hUC-MSCs through tail vein. After 14 days from hUC-MSCs transplantation, rats were euthanized and data were analyzed. HE staining and mean linear intercepts showed that SU5416-treated rats exhibited typical emphysema while emphysematous changes in model rats after hUC-MSCs transplantation disappeared completely and were restored to normal phenotype. Furthermore, hUC-MSCs inhibited apoptosis as shown by TUNEL and Western blotting.ELISA and Western blotting showed hUC-MSCs rescued VEGF-VEGFR2-AKT pathway in emphysematous lungs. Conclusions The findings show that hUC-MSCs effectively repair the emphysema injury. This study provides the first evidence that hUC-MSCs inhibit apoptosis via rescuing VEGF- VEGFR2-AKT pathway in a rat model of emphysema.

Objective: To investigate the effect of intrauterine intervention on severe primary fetal hydrothorax. Methods: Twelve cases with severe fetal primary hydrothorax who underwent prenatal intervention from January 2014 to December 2017 in Guangdong Women and Children Hospital were retrospectively reviewed. Results: The median gestational age of prenatal diagnosis was 30.8 weeks (24.0-33.0 weeks) . All cases were excluded congenital chromosomal abnormalities by prenatal diagnosis, and had no complications of pregnancy during prenatal diagnosis and had hydrothorax. Three cases (3/12) were right hydrothorax, the other 9 cases (9/12) were bilateral. Thoracoamniotic shunting was performed in 7 cases (7/12) . Thoracentesis was performed in 5 cases (5/12) , and the hydrothorax reappeared soon after operation in 4 cases, shunt placement was performed again. The hydrothorax was dissolved in 2 cases, released in 6 cases.Tube falling off occurred in 1 case,treatment was abandoned in 1 case and intrauterine fetal death happened in 1 case, and 1 case wasn′t rechecked by ultrasonic due to premature birth following thoracentesis. In 10 cases who had deliveries, 5 newborns (5/10) were premature, 6 newborns (6/10) underwent assisted mechanical ventilation, 8 newborns (8/10) underwent thoracic close drainage, all of them were discharged when hydrothorax resolved. Conclusions Antenatal intervention may improve the chance of survival in severe primary fetal hydrothorax. Thoracoamniotic shunting is the first-choice for the primary severe fetal hydrothorax.