Objective The various influencing factors in the process of nano-silver preparation were studied in order to prepare nano-silver that has uniformity particle size distribution and good crystallinity. Methods Through the method of liquid reduction at room temperature,the nano-silver powders were prepared directly using silver nitrate as raw material,water as the reactive medium,polyvinylpyrrolidone(PVP) as protecting agent and ammonium formate as reducing agent. By means of EDS and TEM(transmission electron microscopy),the prepared silver particles were characterized. The various influencing factors in the process of preparation were also discussed. Results With the increasing concentration of AgNO3,the particle diameter of nano-silver changed obviously. When the concentration of silver nitrate was 0.25-0.30 mol/L,the size of product is the smallest. With the increasing concentration of reductant,the diameter of nano-silver decreased gradually. While at the concentration of 0.70 mol/L HCOOH,the average diameter of nano-silver is not more than 10 nm. Smaller and more well-distributed silver powder could be prepared at ratio of concentration of PVP to AgNO3 with the value of 1.5-1.0,at which PVP presented effective protection. Conclusions This method can prepare particle of nano-silver which revealed well dispersed and uniform size. The process is stable and reliable,easy to operate,high yields and suitable for industrial production.

BACKGROUND:Articular cartilage has limited ability to repair itself, and the traditional means are difficult to repair articular cartilage defects, but articular cartilage tissue engineering provides new methods and approaches for large-area articular cartilage defects. OBJECTIVE:To review the current status, problems and prospects of tissue engineering technology in articular cartilage repair. METHODS:The retrieval of PubMed database was performed for articles published from 1982 to 2015, with the keywords of “articular cartilage, repair, tissue engineering” in English. Literatures related to tissue engineering repair of articular cartilage were included, but repetitive studies were excluded. Finaly 39 articles were reserved in result analysis. RESULTS AND CONCLUSION:Excelent seed cels have chondrogenic differentiation potential, and currently, the main seed cels for articular cartilage repair include mesenchymal stem cels, embryonic stem cels, adipose-derived stem cels and precartilaginous stem cels. Different growth factors, which can induce the in vivo growth of host parenchymal cels, improve seeded cel stability, and accelerate tissue regeneration, tend to be combined in clinical application. Composite scaffolds are also one of hot researches that can promote cell inoculation and spatial distribution as well as accelerate cell proliferation. To obtain the best effectiveness of articular cartilage repair, how to optimize seed cells, select and match scaffold materials to construct new types of composite patterns is an important direction in the future.

Objective To investigate the effect of sodium demethylcantharidate injection on adherence,invasion and metastasis and to investigate the related mechanism in human hepatocarcinoma cell line HepG2.Methods Adherence ability,migration and invasion of HepG2 cells inhibited by sodium demethylcantharidate injection were assessed by MTT and Transwell techniques.Expression levels of MMP-9 protein in HepG2 cells were determined by immunohistochemistry.Results The number of adhesion,migration and invasion of HepG2 cells were significantly lower in sodium demethylcantharidate injection group than those in the control group (P ＜ 0.05).HepG2 cells co-incubated with sodium demethylcantharidate injection in the concentration of 0.25 μg/ml for 30,60,90 and 120 min showed higher cell adhesion than the control group.The adhesion inhibition ratios were 48.11％,33.81％,28.97 ％ and 16.83 ％,respectively.The migration and invasion inhibition rates were 64.19 ％ and 58.19 ％.With concentration of sodium demethylcantharidate injection to increasing,expression levels of MMP-9 protein in HepG2 cells more and more lower than control group.Conclusion The adherence,migration and invasion abilities of HepG2 cells are markedly inhibited by sodium demethylcantharidate injection,the mechanisms is possible related to the expression levels of MMP-9 protein.

Clinical internship is a key step for training qualified clinician. This article narrates the measures that the hospital have taken to improve administration of clinical internship and teaching quality on the aspect of clinical intern training, examination and quality control.

Objective To observe the effect of octreotide acetate on plasma ETX and serum inflammatory cytokine of the rabbit with hepatic ischcmia reperfusion injury. Methods Pringle's maneuver rabbit hepatic ischemia-repeffusion models were established. 24 adult New Zealand rabbits were random divided into equal 3 groups: sham operative group(group A), iacbemia reperfusion group(group B)and octreotide acetate preconditioning group(group C). Endotoxin (ETX) in the plasma, tumor necrosis factor-alpha (TNF-α) and intcdeukin-1beta (IL-1β) were detected in every rabbit at the time before iachemia (T1), 30min after ischemia (T2), 30min (T3) and 120min (T4) after reperfusion. Results From T2 to T4, the ETX in group B and C were higher than that in group A (P < 0.05), the ETX of group C were lower than that in group B (P<0.05). From T2 to T4, the TNF-α of group B and C were higher than that of group A(P<0.05). From T3 to T4 the TNF-α of group C were higher than that of group A(P<0.05). From T2 to T4,the IL-1β of group B and group C were higher than that of group A(P<0.05), and the IL-1β of group C were lower than that of group B (P<0.05). Conclusion Octreotide acetate can decrease plasma ETX and down-regulate inflammation factors, such as TNFαand IL-1β, in serum of the rabbit with hepatic iacbe-mia-reperfusion injury, which may be the protective mechanism of oetreotide acetate on rabbit hepatic isehemia-reperfusiun injury.

Objective Discusstheinfluence of early diagnosis of myocardial damage and heart-type fatty acid binding protein(h-FABP)on patients with severe head injury after rehabilitation.Methods The sorum h-FABP was detected using enzyme-linked immunosorbent one-step quantitative detection,cTnI using solid-phase enzyme-linked immunosorbent assay(ELISA),CK-MB using the method of immunosuppressive.Results The sorum levels of h-FABP,cTnl and CK-MB in severe head injury patients levels were significantly higher than that of healthy control group(P<0.01),the positive serum h-FABP in severe head injury patients Was significantly higher than cTnI,CK-MB or ECG(P<0.01);8 cases died in47 cases of severe head injury patients,the mortalisty rate was 17.02%(8/47),the mortality rate of abnormal sentrum h-FABP,cTnI and CK-MB group was significantly higher than the normal group(P<0. 01);The serum h-FABP in predicting the death of severe head injury patients was high in sensitivity and negative predictive value,but lower in specificity and accuracy.Condusiom The serum h-FABP quantitative assay as a high-sensitive indicators to minor myocardial dsmage can be used an objective indicators forjudging severity ofacute severe head injury,evaluating incidence of accidents.

Objective To evaluate the reliability and validity of the short form of the Chinese Childbirth Self-Efficacy Inventory (CBSEI-C32) in Haikou,analyze the influencing factors of pregnant women's self-efficacy.Methods The CBSEI-C32 was used to investigate the self-efficacy of the 138 pregnant women in Haikou.Cronbach's α coefficients and confirmatory factor analysis were used to evaluate the reliability and validity of the questionnaire,the influencing factors were analyzed by the univariate analysis and multiple regression analysis.Results The Cronbach's α coefficients for the scale and subscales were 0.973 and between 0.971 and 0.976 respectively;and the cumulative variance was 82.60％,which indicated the scale's reliability and validity was good.The pregnant women's self-efficacy score was (189.64±36.54) points,The times of childbirth,family function and the main coping methods were the influence factor.Conclusions CBSEI-C32 was suit to measure the confidence of natural childbirth in Haikou pregnant women,and their self-efficacy level was general,diversity nursing intervention had to apply for improve pregnant women's self-efficacy.

Objective To study the relationship between the activity of interleukin-4 and interferon-? with steroid effect in children with primary nephrotic syndrome before initiation of steroid therapy. Methods The activity of interleukin-4 and interferon-? in the supernatants of monocytes cell cultures activated with phytohemagglutinin was assessed with enzyme-linked immunosorbentassay in 31 children with primary nephrotic syndrome before iniatiation of steroid therapy. After steroid treatment for 8 weeks, data of steroid sensitivity and resistance were analyzed. Results Before initiation of steroid treament, in steroid sensitivity children with primary nephrotic syndrome an increased proliferative response of interleukin-4 was found (P0.05). Conclusion The activity of interleukin-4 is a useful tool for evaluating the likelihood of steroid sensitivity in children with primary nephrotic syndrome.

Objective To investigate the effect of therapeutic dual-plasmid HBV DNA vaccine on specific humoral and cellular immunity in cancrivorous monkey (Macaca fascicularis). Methods The eukaryotic expression plasmids encoding human IL-2 and IFN-? fusion protein (pFP) were constructed to enhance the cellular immunity of therapeutic HBV DNA vaccine (pS2.S) encoding HBV envelope middle protein in the form of dual-plasmid (pS2.S+pFP). Thirty cancrivorous monkeys were randomly divided into 5 groups (6 each) with sex rotio of 1∶1, namely the dual-plasmid DNA vaccine group in high dosage (2000?g/kg), medium dosage (660?g/kg), low dosage (200?g/kg), and EP-mediated pFP (330?g/kg) or PBS administration groups as the controls. The monkeys were vaccinated repeatedly with the dual-plasmid HBV DNA vaccine, and then the immune responses including level of serum anti-HBs and number of IFN-? secreting T-cells induced by HBsAg were examined by means of enzyme-linked immunosobent assay (ELISA) and enzyme-linked immunosobent spot assay (ELISPOT) respectively. Results Ten weeks after immunization of HBV DNA vaccine, various levels of serum anti-HBs was detected in all the monkeys of three different dose groups. Sixteen weeks after administration of EP-mediated HBV DNA vaccine, the positive HBsAg-specific INF-? T cell responses was found in 3, 2 and 3 out of 3 monkeys, respectively in the high, medium and low losage groups, and HBsAg-specific INF-? T cell responses were positive in all the animals with the respective cell count of 30.0?13.5 SFCs/3?105 PBMCs, 30.7?26.3 SFCs/3?105 PBMCs and 17.7?6.4 SFCs/3?105 PBMCs in each corresponding group at the 29th week. However, HBsAg-specific INF-? T cell responses were negative in the pFP and PBS group at the same time. Conclusion Electroporation-mediated vaccination of the HBV DNA vaccine can effectively induce both humoral and cellular HBsAg-specific immune responses in cancrivorous monkeys.

AIM:The aim of this study was to observe and compare the therapy effect of different kinds of embryonic stem cells(ESCs) on pulmonary injury of mice exposed to bleomycin.These embryonic stem cells were C57BL/6J-ESC,S8-ESC and human-ESC.METHODS:(1) Fifty C57BL/6J female mice were divided randomly into five groups,which were blank control group,bleomycin model group,bleomycin model injected with C57BL/6J-ESC group,bleomycin model with S8-ESC group and bleomycin model with human ESC group.Every group has ten mice.(2) The mice of control group were administrated 0.9% sodium chloride solution and the mice in other four groups were administrated bleomycin intratracheally.Three different kinds of ESCs were administrated to the mice in three different ESCs-treated groups respectively one hour after bleomycin exposure.The life-span and hydrocyproline concentration were examined.The pathologic changes of the lung and the engraftment of the ESCs in the injured lung were observed.RESULTS:(1) The death rate in three different ESCs-treated groups declined much more obviously than that in the control group on 8 days after bleomycin exposure(bleomycin model group 50%,C57BL/6J-ESC group 37.5%,S8-ESC group 20%,human-ESC group 20%).(2) The extent of pathologic changes of the lung in S8-ESC group was lighter significantly than that in the bleomycin model group,but in C57BL/6J-ESC group and human-ESC group,their pathologic changes were similar to that in bleomycin model group.(3) The hydrocyproline concentration in S8-ESC group was lower distinctly than that in bleomycin model group(P0.05).(4) The positive signals of three kinds of ESCs could be found in the lung at 1,3,12 and 24 hours after the stem cells were administrated,but signals were the strongest 3 hours after stem cells were given.All of the signals disappeared three days later.CONCLUSION:S8-ESCs transplantation can improve the tolerence of mice to bleomycin and ameliorate acute lung injury.