1.Clinical analysis of 34 cases of candidemia
Xudong XIONG ; Fang XIE ; Huiping YAN
Chinese Journal of Practical Internal Medicine 2006;0(19):-
Objective To search for clinical appearance and fungal resistance of candidemia in ICU.Methods Thirty-four cases of candidemia in ICU during 2003and 2006 were analyzed retrospectively.Results Twelve of 34 cases of candidemia were C.albicans,and 22 of 34 cases were non-C.albicans,which were 64.71% in candida monilia.Fever and chill of candidemia were 100% and 70.59% differently.Conclusion If there is high risk factor of fungous infection in clinic,at the same time fever and chill appear,and antibiotics treat is inefficacy not effective,it is possible to consider candidemia.
2.Analysis of sputum bacterial culture in pneumoconiosis patients
Yanying CHEN ; Huiping XIE ; Shuying XIE ; Shiyong WEN
International Journal of Laboratory Medicine 2015;(11):1494-1495,1497
Objective To analyze sputum culture results in pneumoconiosis patients ,to provide reference for the prevention , treatment and rational drug use of pneumoconiosis .Methods A total of 122 sputum samples from pneumoconiosis patients were collected to do sputum bacterial culture and drug sensitive test .Results The infection rates of Ⅱ ,Ⅲ pneumoconiosis patients were significant higher than that of Ⅰ pneumoconiosis patients (P< 0 .05) .The infection rates of pneumoconiosis associated with tuber‐culosis was significant higher than that of patients only with pneumoconiosis (P< 0 .05) .Conclusion It is necessary to use drug ra‐tionally to eliminate or avoid the abuse of antibiotics ,in order to improve the treatment effect of pneumoconiosis .
3.Quantitative Determination of Levofloxacin by PVC Membrane Ion-Selective Electrode
Huiping XI ; Deguang ZHANG ; Yanzhao LIU ; Hongtao XIE
China Pharmacy 2007;0(34):-
OBJECTIVE:To determine the content of levofloxacin by PVC membrane selective electrode. METHODS: A glass electrode coated with levofloxacin PVC membrane was prepared for the first time with the molecular complex of levofloxacin iodide and bismuth iodide as electric active material, and a series study was performed on its responsibility. RESULTS: The electrode gave Nernst response to levofloxacin over the concentration range of 5.0?10-3~1.0?10-5 moL?L-1 with a slope rate of 56.5mV?pC-1, a suitable pH of 2.5~4.0, and an average recovery rate of 98.31%~101.6%(2.80%~4.90%). CONCLUSION: The electrode was simple in operation, and it has a rapid response and good reproducibility, and it is applicable for the quantitative determination of levofloxacin.
4.Status of nursing professional environments and countermeasures
Jingyu XIE ; Yumei LIANG ; Huiping LIANG ; Xiaorong HUANG
Modern Clinical Nursing 2016;15(2):42-45
Objective To investigate the present situation of nursing professional environments in our hospital and provide theoretical basis for mapping out relevant countermeasures. Method A questionnaire survey was conducted among 338 nurses in our hospital by using the general information questionnaire and practice environment scale (PES). Result The dimensions of PES by their average scores in descending sequence included leadership and ability of nurse administers, doctor-nurse cooperation, foundation for high quality care nurses′participation in hospital affairs, and sufficient manpower and resources. Conclusion In order to provide good and healthy work environment for nurses, nursing administrators should provide enough nursing manpower resoures and apply excitation mechanism in their hospital.
5.Analysis on the effect of metoclopramide combined with psychological intervention on nausea and vomiting after laparoscopic cholecystectomy
Minping SHEN ; Xinfang XIE ; Huiping YU ; Liufen SHEN
Chinese Journal of Biochemical Pharmaceutics 2017;37(8):374-375,377
Objective To investigate the effect of metoclopramide combined with psychological intervention on nausea and vomiting after laparoscopic cholecystectomy (LC). Methods From January 2015 to January 2017, 90 patients with nausea and vomiting after laparoscopic cholecystectomy according to the different treatment and nursing intervention mode were divided into the control group and the observation group, 45cases in each group. The control group were given granisetron and routine nursing, the observation group were received metoclopramide and psychological intervention. The experimental data was recorded and compared, the improvement of nausea and vomiting were observed. Results The clinical effect in the observation group is better than that in the control group. The improvement of nausea and vomiting, the nursing satisfaction in the observation group were better than those in the control group. The differences were statistically significant (P<0.05). Conclusion Metoclopramide combined with psychological intervention on the treatment of the patients with nausea and vomiting after laparoscopic cholecystectomy, curative effect is obvious, which can effectively improve the nausea and vomiting, nursing satisfaction is high, which is worthy of clinical application.
6.Teaching experience of molecular biology experiment for the international students
Baohong LI ; Xu HE ; Yanzhi HOU ; Huiping MA ; Lin SUN ; Qianchi XIE ; Xi CHEN
Basic & Clinical Medicine 2009;29(11):1230-1232
Since initiation of training on laboratory of molecular biology in my school, we accumulated experience in teaching process especially among international students and application of pedagogy as well as technology. Strengths, weakness and opportunities are analyzed to improve teaching quality.
7.Effect of pelvic muscle exercise on quality of sexual life after panhysterectomy surgery
Juan XIE ; Huiping FAN ; Jingjing CHEN ; Meiyun SHEN ; Hongying FENG ; Yan SONG
Chinese Journal of Practical Nursing 2014;30(26):60-63
Objective To evaluate the effect of pelvic muscle exercise on quality of sexual life after panhysterectomy surgery.Methods A retrospective review was made on 166 patients with integrated follow-up documents,after their panhysterectomy surgery caused by benign diseases.A normal nursing care was provided to the control group (n=81); while an additional pelvic muscle exercise was performed on the normal care basis,to those in the experimental group (n=85).Finally,a questionnaire on sexual life quality was made at the sixth month,ninth month,and twelfth month respectively,using a repeated measure ANOVA to analyze the data of each dimension and the whole score.Results Significant differences revealed both on the whole score and dimensions of the sexual satisfaction,sexual communication,sexual anxiety and sexual reaction,in different time sites.However,no difference was found in sexual attitude and body image.Besides,a cross interaction was found between time and intervention,where intervention presented different effects.Conclusions Pelvic muscle exercise is recommended in gynecological continuing nursing care to facilitate the recovery of sexual life after panhysterectomy surgery.
8.Radial scanning endoscopic ultrasonography for preoperative grading of esophageal cancer
Kaifeng HU ; Qiao MEI ; Jianming XU ; Huiping CHAI ; Huijun XIE ; Naizhong HU ; Guifang YANG
Chinese Journal of Digestive Endoscopy 2010;27(12):629-631
Objective To evaluate mechanical radial scanning endoscopic ultrasonography (EUS) for preoperative tumor and lymph node ( TN ) staging of esophageal cancer. Methods From January 2010 to June 2010, a total of 60 patients with esophageal cancer underwent preoperative staging with mechanical radial scanning EUS. The findings of EUS were compared with postoperative pathological outcomes. Results EUS accurately predicted T stage in 80 % of cases and N stage in 71% cases. Sensitivities to T1 , T2 , T3 and T4 were 75% , 100% , 96% and 50% , respectively, and those to N0 and N1 were 55% and 100% , respectively. With exclusion of 11 patients with un-passable lesions, the accurate rate of EUS in T staging of focal and advanced cancers was 90% ( 44/49 ). Conclusion Mechanical radial scanning EUS can accurately predict T and N stages in preoperative patients with esophageal cancer, which also exhibits high differential accuracy in focal and advanced esophageal cancer.
9.Influence of ovariectomy and estrogen replacement on gene expression profile of myocardium in rats
Meilian LIU ; Xia XU ; Ping XIE ; Jin LU ; Shuhua CHEN ; Weimin ZENG ; Huiping SONG
Chinese Journal of Pathophysiology 1999;0(09):-
AIM:To investigate the influence of ovariectomy and estrogen replacemeot treatment on profile of gene expression in myocardium by cDNA microarray,and to characterize the targeting genes of estrogen.METHODS:cDNA microarray containing 1 400 rat cDNAs was used to study the genes differentially expressed in myocardium between sham(Ⅰ),ovariectomy(Ⅱ,OVX)and estrogen replacement treatment(Ⅲ,OVX+E2)group.Then down-regulated genes in myocardium of OVX rats were further confirmed by RT-PCR.RESULTS:177 genes were differentially expressed in myocardium between sham and OVX rats,with 91 genes up-regulated and 86 genes down-regulated in OVX rats.164 genes were differentially expressed in myocardium between OVX and OVX+E2 rats,with 113 genes up-regulated and 54 genes down-regulated in OVX rats.There were 54 genes differentially expressed in OVX compared to sham and OVX+E2.They are involved in membrane channels and transporters(18),cell receptors(9),intracellular transducers/effectors/modulator(7)and metabolism(6).Most of the genes(45)were down-regulated in OVX rats and up-regulated in OVX+E2 rats.RT-PCR test confirmed the results of cDNA microarray.CONCLUSIONS:Long-term estrogen replacement may influence the expression of genes involved in membrane channels and transporters,cell receptors,intracellular transducers/effectors/modulator and metabolism.Long-term estrogen replacement has some beneficial effects on ionic concentration and cardiac function which partially comes from the results of influence of expression on Na+,K+-ATPase and Na+/H+ exchanger.Estrogen has an inhibitory effect on the expression of dopamine receptor,which partially clarify the myocardial protection of estrogen.
10.Regulatory mechanism in expression of IRS-1 and 2 by estrogen and high concentration of insulin
Ping XIE ; Meilian LIU ; Weimin ZENG ; Jianjun HUANG ; Shuhua CHEN ; Jin LU ; Xia XU ; Huiping SONG
Chinese Journal of Pathophysiology 1986;0(01):-
AIM: To study the molecular mechanism in modulation of expression of insulin receptor substrate-1 and-2 (IRS-1,-2) by estrogen and high concentration of insulin. METHODS: The 5′-regulatory regions of IRS-1 and IRS-2 gene were cloned into the pGL3 plasmid with luciferase reporter, and the clones were transfected into HeLa cells. The cells were incubated with estradiol (1 nmol/L) and high concentration of insulin (100 nmol/L). The relatively transcriptional activity of the 5′-regulatory regions of IRS-1 and IRS-2 gene was detected. RESULTS: It was found that the relatively transcriptional activity of the 5′-modulatory regions of IRS-2 reduced markedly after cells were incubated with 100 nmol/L insulin (P