Some measures have been taken such as adjusting validated experiment,establishing and launching an integrated designing experiment for biopolymers,circumfusing experimental correlational content,discussing the problem-based learning method,assorting home-made multimedia teaching material and combining proper motion designing biochemical experiment in a lot of biochemical experimental teaching to train the basic skills of the high-level talents in medicine.

Objective To assess the effect of the intensive insulin therapy for preventing the intensive care unit acquired weakness systematically ,in order to provide reference for making the intervention strategy .Methods We searched the database CBM ,CNKI ,WanFang Data ,VIP ,PubMed ,Cochrane Library ,EMBASE and ISI from the day of establishment to Dec 2014 to find the relevant studies with computer and by hand .Two researchers selected the studies ,evaluated the quality of the articles and ex‐tracted data according to the rigorous criterion ,then we performed the Meta‐analysis on the data with the software RevMan5 .3 .Re‐sults Three articles were enrolled ,2 788 patients in the intensive care unit were recruited ,including 1 380 patients were from the experimental group and 1 408 patients came from the controlled group .The result of the Meta‐analysis showed that compared to the conventional insulin therapy ,intensive insulin therapy reduced the incidence of intensive care unit weakness(RR= 0 .61 ,95% CI:0 .42-0 .89 ,P=0 .01) ,but there was no statistical significance of it on the the intensive care unit mortality(RR=0 .74 ,95% CI:0 .47-1 .17 ,P=0 .20) ,the hospital mortality(RR=0 .81 ,95% CI:0 .58 -1 .13 ,P= 0 .22) ,the duration of intensive care unit stay (MD=0 ,95% CI:-0 .37-0 .37 ,P=1 .00) and the duration of mechanical ventilation(MD= -1 .48 ,95% CI:-3 .43-0 .47 ,P=0 .14) . Conclusion Intensive insulin therapy can reduce the incidence of intensive care unit acquired weakness ,but the effect of it on the intensive care unit mortality ,hospital mortality ,duration of intensive stay and duration of mechanical ventilation needs further research to verify .

ObjectiveTo study the effects of painful inter-stimulus interval on event-related potentials in rabbits for best inter-stimulus interval.MethodsElectrode was attached to F4 site on rabbits' skull. An Oddball paradigm stimulus were adopted. The waveforms recorded under different inter-stimulus interval were analysed.ResultsThe latency of pain evoked potentials P2,N2,P3a under different inter-stimulus interval showed no significant difference.The amplitude was highest when the interval was 8s.ConclusionsThere is a best inter-stimulus interval for pain evoked potential.

0.05), but it increased writhing times of mice (P0.05), but HPPT was decreased(P

Objective To explore the effect of labels management on high-risk drugs. Methods Labels management group was established, to be responsible for use and storage of high-risk drugs. The management methods included taking analysis on adverse nursing events, designing and making labels in drug storage and use, and making a field survey on adverse nursing events before and after the management. Result After manipulation of the labels management, the number of adverse nursing events of drug use fell from 35 to 0. Conclusion Drug storage and use with special, standard labels is effective in preventing nursing adverse events in high risk drugs.

Objective To evaluate the value of hyperbaric oxygen treatment on patients with traumatic brain injury and to explore the possibility of C-reactive protein(CRP) as a index for estimating the effect of hyperbaric oxygen treatment on patients with traumatic brain injury.Methods 70 patients with traumatic brain injury were divided into two groups by randomization:hyperbaric oxygen treatment group(group A) and control group(group B).The recovery of patients was evaluated with Glasgow Coma Scale(GCS),while the content of serum CRP in 70 patients was measured before and after a course of treatment.Results ⑴After a course of treatment,GCS of group A was (11 9?1 7) scores,while GCS of group B was(10 7?2 0) scores,there was significantly difference between two groups(P

Objective Discusstheinfluence of early diagnosis of myocardial damage and heart-type fatty acid binding protein(h-FABP)on patients with severe head injury after rehabilitation.Methods The sorum h-FABP was detected using enzyme-linked immunosorbent one-step quantitative detection,cTnI using solid-phase enzyme-linked immunosorbent assay(ELISA),CK-MB using the method of immunosuppressive.Results The sorum levels of h-FABP,cTnl and CK-MB in severe head injury patients levels were significantly higher than that of healthy control group(P<0.01),the positive serum h-FABP in severe head injury patients Was significantly higher than cTnI,CK-MB or ECG(P<0.01);8 cases died in47 cases of severe head injury patients,the mortalisty rate was 17.02%(8/47),the mortality rate of abnormal sentrum h-FABP,cTnI and CK-MB group was significantly higher than the normal group(P<0. 01);The serum h-FABP in predicting the death of severe head injury patients was high in sensitivity and negative predictive value,but lower in specificity and accuracy.Condusiom The serum h-FABP quantitative assay as a high-sensitive indicators to minor myocardial dsmage can be used an objective indicators forjudging severity ofacute severe head injury,evaluating incidence of accidents.

0.05), while strychnine and naloxone obviously lengthened the sleeping time of the mice injected with thiopental sodium (P

Objective To investigate effects of intrathecal injection of sufentanil combined with bupivacaine on postoperative analgesia and immunity function in elderly patients under lower extremity orthopaedic surgery.Methods Totally 60 elderly patients with ASA Ⅰ or Ⅲ under lower extremity orthopaedic surgery were randomly divided into two groups:bupivacaine and sufentanil group (each n =30).3 ml bupivacaine (2 ml 0.75％ bupivacaine plus 1 ml 0.9％ normal saline) were given in bupivacaine after successful spinal anesthesia and 3 ml sufentanil (1 ml 0.75 ％ bupivacaine plus 1 ml 0.9％ normal saline and 1 ml with 5 μg sufentanil) were used in sufentanil group respectively.The anesthetic feeling and block level before operation recovery time were recorded postoperation.Visual analogue scale (VAS) and adverse reaction were recorded at 1,6,12,24 and 48 h after surgery.Cellular and humoral immunity function were recorded at the following time points:T0,the day before surgery; T1,the day after surgery; T2,the first 5 postoperative days; T3,the first 10 postoperative days; T4,the first 15 postoperative days.Results The sensory and motor block levels in sufentanil group were T9～～T10 and T11～T12 respectively,which were higher than in bupivacaine (T7～T8 and T8～T10).The recovery time in sufentanil group was shorter than in the bupivacaine (P＜0.05).There were no significant differences in VAS scale between the two groups at any postoperative time points (P＜0.05).The incidence of skin pruritus was higher in sufentanil group (6 cases) than in bupivacaine (P＜0.05).There were significant differences in the indexes for the immune condition including CD3+,CD4+/CD3+,CD4/HLADR,CD8/HLADR,B-cell,NK-cell between time points of T2[(48.7±2.3)％,(33.4±1.8)％,(10.2±1.8)％,(0.75±0.07)％,(1.02±0.16)％,(3.12 ±0.19) ％,(2.53±0.14)％,respectively] and T0 in bupivacaine (P＜0.05).There were significant differences in the indexes for the immune condition including CD3+,CD4+/CD3+,CD4/HLADR,CD8/HLADR,B-cell,NK-cell at T2 between sufentanil group [(54.5 ± 2.4) ％,(36.3 ± 1.7) ％,(15.1±1.6)％,(1.32±0.11)％,(1.25±0.14)％,(4.96±0.25)％,(4.17±0.10)％,respectively] and bupivacaine (P＜0.05).Conclusions Intrathecal injection of 5 μg sufentanil combined with low concentration bupivacaine can be safely and effectively applied in elderly patients under lower extremity orthopaedic surgery,and can reduce local anesthetic concentration,shorten recovery time of anesthesia and accelerate the recovery of postoperative immunity function.

Objective:To determine the effect of IL-12 on the cellular immune response of PBMC from patients with chronic hepatitis B virus infection, and provide basic scientific information for clinic therapy of this disease.Methods:PBMCS were prepared from peripheral blood of individuals with chronic HBV infection and cultured in the presence or absence of HBsAg and HBcAg with or without IL-12.The level of IFN-?in culture supernatants, the frequency of IFN-?-producing cells, and the subpopulation of IFN-?-producing cells were detected by either ELISA,ELISPOT or FACS.Results:Less than 30% patients and very low level of IFN-? were observed when PBMCs were stimulated with HBsAg or HBcAg alone. Addition of IL-12 to the cultures resulted in significant increase in IFN-?production and IFN-?-producing cells. In addition, IL-12 induced expression of IFN-? not only by CD8~+T cells, but also by non-T cell populations.Conclusion:IL-12 can promote the cellular immune response to the chronic hepatitis B virus by the enhancement of IFN-?production.