Chemotherapy-induced amenorrhea(CIA) is a objective process,the reason is clear basically,for patients with hormonal receptor positive will improve disease free survival and overall survival when develop CIA,so we can prevent ovarian function for patients have lower risk and intend to keep bearing ability;and further treatment inhibit ovarian function for improving outcome in patients with high risk and poor prognosis without CIA developed;CIA is an objective and simply observed factor.

Objective To discuss the efficacy of ambroxol hydrochloride inhalation in treatment of infantile pneumonia.Methods Totally 80 pneumonia infants were selected and randomly divided into two groups.The control group (39 cases) were given anti-infection,cough-relieved,and other treatment.The observation group (41 cases) were given ambroxol hydrochloride inhalation on the basis of control group treatment.The efficacy of ambroxol hydrochloride inhalation in treatment of infantile pneumonia was evaluated by efficacy,clinical symptoms disappearing time,red blood cell hematocrit,blood viscosity,and adverse reaction during treatment.Results After treatment,the effective rate of observation group was higher than that of control group (P ＜ 0.05).There were 38 infants in observation group and 28 infants in control group with cough and dyspnea alleviated and phlegm reduced.After atomizing inhalation of ambroxol hydrochloride,the cough,pulmonary rales disappearing time and hospitalization time were significantly shortened (P ＜ 0.05).After treatment,the red blood cell hematocrit and blood viscosity of observation group was lower than that of control group (P ＜ 0.05).During treatment,there was no statistical significance on adverse reaction between two groups.Conclusion Ambroxol hydrochloride inhalation has a good curative effect on infantile pneumonia.It could improve the symptoms of pneumonia and reduce blood viscosity with good safety,which is worthy of clinical use.

Objective To assess the effect of the intensive insulin therapy for preventing the intensive care unit acquired weakness systematically ,in order to provide reference for making the intervention strategy .Methods We searched the database CBM ,CNKI ,WanFang Data ,VIP ,PubMed ,Cochrane Library ,EMBASE and ISI from the day of establishment to Dec 2014 to find the relevant studies with computer and by hand .Two researchers selected the studies ,evaluated the quality of the articles and ex‐tracted data according to the rigorous criterion ,then we performed the Meta‐analysis on the data with the software RevMan5 .3 .Re‐sults Three articles were enrolled ,2 788 patients in the intensive care unit were recruited ,including 1 380 patients were from the experimental group and 1 408 patients came from the controlled group .The result of the Meta‐analysis showed that compared to the conventional insulin therapy ,intensive insulin therapy reduced the incidence of intensive care unit weakness(RR= 0 .61 ,95% CI:0 .42-0 .89 ,P=0 .01) ,but there was no statistical significance of it on the the intensive care unit mortality(RR=0 .74 ,95% CI:0 .47-1 .17 ,P=0 .20) ,the hospital mortality(RR=0 .81 ,95% CI:0 .58 -1 .13 ,P= 0 .22) ,the duration of intensive care unit stay (MD=0 ,95% CI:-0 .37-0 .37 ,P=1 .00) and the duration of mechanical ventilation(MD= -1 .48 ,95% CI:-3 .43-0 .47 ,P=0 .14) . Conclusion Intensive insulin therapy can reduce the incidence of intensive care unit acquired weakness ,but the effect of it on the intensive care unit mortality ,hospital mortality ,duration of intensive stay and duration of mechanical ventilation needs further research to verify .

Objective To derive hematopoietic stem cells with functional properties of hematopoietic reconstitution from murine embryonic stem (ES) cells. Methods ES-D3 cells by formation of the day-4 embryoid bodies (4dEBs) were induced into hematopoietic stem cells by co-culture with murine bone marrow endothelial cell-conditional medium (mBMEC-CM) and the fetal liver stromal cell-conditional medium (FLSC-CM). This experiment was designed to 4 groups (mBMEC-CM + FLSC-CM group, mBMEC-CM group, FLSC-CM group, and the control group). Results The total cell numbers, CD34 + cell numbers, and colony numbers formed in the mBMEC-CM + FLSC-CM group were the highest among the 4 groups. The cells in the mBMEC-CM + FLSC-CM group resumed the hematopoietic system of the mice after being transplanted with the inducing cells. Conclusion The culture condition combing mBMEC-CM with FLSC-CM can promote murine ES cells differentiating into hematopoietic stem cells with functional properties of hematopoietic reconstitution.

Objective To study the relationship between the activity of interleukin-4 and interferon-? with steroid effect in children with primary nephrotic syndrome before initiation of steroid therapy. Methods The activity of interleukin-4 and interferon-? in the supernatants of monocytes cell cultures activated with phytohemagglutinin was assessed with enzyme-linked immunosorbentassay in 31 children with primary nephrotic syndrome before iniatiation of steroid therapy. After steroid treatment for 8 weeks, data of steroid sensitivity and resistance were analyzed. Results Before initiation of steroid treament, in steroid sensitivity children with primary nephrotic syndrome an increased proliferative response of interleukin-4 was found (P0.05). Conclusion The activity of interleukin-4 is a useful tool for evaluating the likelihood of steroid sensitivity in children with primary nephrotic syndrome.

OBJECTIVE:To investigate the effects of Bupleuri plus Saliva miltiorrhiza on preventing the formation of hepatic fibrosis in rats METHODS:To establish an animal model of hepatic fibrosis by feeding high fat diet and 10% alcohol and subcutaneously injecting CCl4 and to detect the zymogram and biochemical parameters and observe the pathological changes of liver tissue RESULTS:Bupleuri and Saliva miltiorrhiza could significantly depress the activities of ALT,AST,MAO,ALP and had no influence on the ?-GT in serum,the serum TG was decreased and TG in tissue was obviously raised and serum GSH was elevated in hepatic fibrosis rat The extract could significantly decreased the levels of PC Ⅰ and PC Ⅲ in hepatic fibrosis rat The histopathological examination showed that the extract could diminish liver degeneration and necrosis,and block the formation of fiber diazoma,the degree of collagen proliferation was significantly lower than that of the control group CONCLUSION:Bupleuri and Saliva miltiorrhea extract could effectively protect the liver cell,and could retard the formation of hepatic fibrosis as well

We investigated the clinical and photographic characteristics of uremic lung and review the associated literature,so as to improve the diagnostic and therapeutic abilities of uremic lung.The clinical symptoms and signs together with the photographic characteristics of the patient who was diagnosed as uremic lung complicated with pulmonary infection and congestive heart failure in our division were analysed and the associated literature was reviewed.The patient was admitted for the complaint of cough,expectoration and dyspnea.He was diagnosed as chronic renal failure with pulmonary infection and congestive heart failure.The symptoms alleviated after adequate hemodialysis and antibiotic therapy.However,mild dyspnea remained with photographic examination still showing consolidation in the lungs.The diagnosis of uremic lung was established after the exclusion of pulmonary infections of other pathogens and tumor.After 8-month maintenance hemodialysis,the pulmonary lesions were thoroughly absorbed.Uremic lung is a common complication of end-stage renal failure.The diagnosis is established after the exclusion of cardiac pulmonary edema,pulmonary infections and tumors.Sufficient hemodialysis is the most adequate treatment for uremic lung.Abstract:SUMM ARY W e investigated the clinical and photographic characteristics of urem ic lung and review the associated literature,so as to improve the d iagnostic and therapeutic abilities of urem ic lung.The clinical symptoms and signs togetherwith the photographic characteristics of the patientwho was d iagnosed as ure-m ic lung complicated with pulmonary infection and congestive heart failure in our d ivision were analysed and the associated literature was reviewed.The patientwas adm itted for the complaint of cough,expecto-ration and dyspnea.He was d iagnosed as chronic renal failure with pulmonary infection and congestive heart failure.The symptoms alleviated after adequate hemod ialysis and antibiotic therapy.However,m ild dyspnea remained with photographic exam ination still showing consolidation in the lungs.The d iagnosis of urem ic lung was established after the exclusion of pulmonary infections of other pathogens and tumor.Af-ter 8-month maintenance hemod ialysis,the pulmonary lesionswere thoroughly absorbed.Urem ic lung is a common complication of end-stage renal failure.The d iagnosis is established after the exclusion of card iac pulmonary edema,pulmonary infections and tumors.Sufficient hemod ialysis is the most adequate treat-ment for urem ic lung.

Objective: To investigate the action and mechanism of Bupleurum chinense SD and Schisandra chinensis Turcz extract on liver fibrosis in rat.Methods: The model was prepared by injection of 40% CCl 4 plant oil solution, then the enzyme active AST、AST、MAO、ALP、r-GT、TG in serum and MAO、ALP、GSH、TG in hemogeneous were measured by enzyme method, the level of Pc-Ⅰand PcⅢ were measured by radio-immunity. Results: Bupleurum chinense and Schisandia chinensis extract can reduce significantly enzyme active of ALT、AST、TG、MAO in serum of fibrosis rat, the GSH was elevated and the r-GT was not effect in serum, but it could reduce the level of MAO、ALP、Pc-Ⅰ、Pc-Ⅲ in the homogeneous, sometime the TG was increased and the GSH was not effect in homogeneous. The histopathology showed that Bupleurum chinense and Schisandia chineusis extract could get damaged liver cell back and reinforce the regeneration of hepatic cell. Conclusion:Bupleurum chinense and Schisandid chinensis extract the action of protective and restoration on injured liver cell, and its could eliminate the bile accumulation, to retard the fatty degeneration in fibrotie rat.

Objective To construct a subtractive cDNA library of genes transactivated by XTP3 protein with suppression subtractive hybridization technique in order to clone genes associated with transactivation. Methods mRNA was isolated from HepG2 cells transfected by pcDNA3.1(-)-XTP3 and pcDNA3.1(-) empty vector respectively, and then cDNA was synthesized. After restriction enzyme Rsa Ⅰ digestion, small sizes cDNAs were obtained. Then tester cDNA was divided into two groups and ligated to the specific adaptor 1 and adaptor 2 respectively. After being hybridized with driver cDNA twice and underwent two times of nested PCR, tester cDNA was subcloned into T/A plasmid vectors to set up the subtractive library. Amplification of the library was carried out with E.Coli strain JM109. The cDNA was sequenced and analyzed in GenBank with Blast search after PCR. Results The amplified library contained 30 positive clones. Colony PCR showed that 23 clones contained 200-1 000bp inserts. Sequence analysis was performed also. It was found that 20 kinds of known and 2 kinds of novel cDNA sequences may be target genes transactivated by XTP3 protein. Conclusion The subtractive library of genes transactivated by XTP3 protein was constructed successfully. It provides a foundation for the further research on pathogenesis of the viral proteins.