Objective To study the efficiency of endoscope-guided Savary bougie dilatation ( ESBD ) for strictured duodenal ulcer. Methods Forty-five patients with strictured duodenal ulcer were treated with endoscope-guided Savary bougie dilatation. The items of investigation were; easiness of passing the endo-scope through the strictured portion of duodenum, resolving of their symptoms and the complications happened after dilatation. Results The symptoms remitted and endoscope was advanced to descending portion of the duodenum through the stricture in all of 45 patients. The follow up period ranged from 6 months to 36 months. The rates of symptomatic remission were 97. 8% (44/45 ) , 97. 8% (44/45 ) , 94. 4% (34/36) , 93.7%(30/32) , 91.7% (22/24) and 87.5% (14/16) , at 6,12,18,24,30 and 36 months respectively. Recurrence of symptoms in patients without therapy was significantly higher than those with therapy. Conclusion ESBD is a safe and efficient procedure in treating the strictured duodenal ulcer.

Objective To investigate endoscopic clipping in treating acute gastroduodenal perforation. Methods Endoscopic clipping was carried out in 22 patients with acute gastroduodenal perforation as the complication of peptic ulcer or endoscopy. Results The clipping was successful in 19(86. 4% ) of 22 patients with acute gastro duodenal perforation, and surgical therapy was performed in 3(13. 6% ) of 22 cases. A single clipping was performed in 14(73. 7% ) of 19 patients resulted in satisfactory closure of perforation. An additional clipping was needed in 5(26. 3% ) patients 24 h after the first endoscopes procedure. The effectiveness of endoscopic clipping was related to location, cause and size of the perforation. Conclusion Endoscopic clipping is an effective method for acute gastroduodenal perforation as the complication of peptic ulcer or endoscopy.

Objective To investigate the expression and characteristics distribution of ciliary neurotrophic factor (CNTF) and its receptor during the development of retina of healthy Sprague-Dawley(SD)and Royal College of Surgeons (RCS) rats with hereditary retinal degeneration. Methods The expression and distribution of ciliary neurotrophic factor and its receptor were detected by immunohistochemical staining in the retinal paraffin sections of SD and RCS rats from newborn to 12 moths old. Results In the normal retina of SD rats 0-7 days after birth, positive CNTF staining was found in all of the retinal layers and the staining of ganglion cells strengthened and other cells weakened as the age of rats increased; the staining of ganglion cells reached the peak at the 4th week and lasted till the agedness. The same results of the CNTF staining were also found in RCS rats retina. Weak positive staining of CNTFR in all of the retinal layers was seen in the 0-3-day-old SD rats; the ganglion cells were darkly stained and incontinuous positive staining at the site which would develop to be the external segment was found; as the age increased, the positive staining of external segment of photoreceptor enhanced and reached the peak at the 14-28th day after birth. At the 56th day, the staining of ganglion cells in retina of SD rats was strengthened while the staining of external segment weakened till the agedness. The expression of CNTFR in retina of 3-14-day-old RCS rats was the same as which of normal SD rats basically, but the staining of external segment weakened obviously from the 21st day on, and negative staining of external and positive ganglion cells were detected at the 28th day till the agedness. Conclusions Expression of CNTF in normal SD rats and RCS rats with hereditary retinal degeneration is almost the same. The presence of significant difference of expression of CNTFR between normal SD rats retina and RCS rats retina may provide the experimental gist for the CNTF treatment to retinal degeneration.

0.05). Significant difference existed between the patients and endoscopy-negative (pathological reflux) and endoscopy-negative (physiological reflux) (P

Objective:To study the effects of hyperthermia on apoptosis in human colonic cacinoma cell line Lovo. Methods:Lovo cells were exposed to hyperthemia and apoptosis was measured with fluorescent Hocehst 33258 staining, electropherogram gel, flow cytometry (FCM). Results:Significant changes in cell morphology , apoptosis peak apeared before the G1 phase and DNA ladder was observed in various group cells which were exposed to different temperature and in different time. Most significent changes were observed in cells exposed to 43℃ for 3,4 h and 44℃ for 1,2 h. Also cell cycle was found changed. Conclusion:Hyperthermia can cause apoptosis in human colonic cacinoma cell line Lovo, and has phase-specificity.

BACKGROUND:Longitudinal study of changes in obesity is an important method to explore the etiology, which can provide scientific basis for preventing and control ing obesity.
OBJECTIVE:To investigate the effect of age, observation period and birth cohort on the obesity prevalence of adults in Jiangsu province through the age-period-cohort analysis.
METHODS:20-69-year-old adults in Jiangsu province were col ected as the research objects. The stratified cluster sampling method was used to col ect the obese data in 2000-2010, and analyzed with SAS software. RESULTS AND CONCLUSION:Obesity prevalence of 1946-1950 birth cohort to 1976-1980 birth cohort was gradual y increased (P<0.05) from 2000 to 2010. Obesity prevalence from 1931-1935 birth cohort to 1941-1945 birth cohort was not significantly increased (P>0.05). With the increasing age in each age group of over 25 years old, the risk of obesity was increased gradual y. There were significant differences in the odds ratios between the baseline groups of 20-25 years old and the age groups of over 25 years (P<0.05). Compared with the 1995-1999 birth cohort, the other periods had a slight influence on the obesity prevalence (P>0.05). Compared with 1976-1980 birth cohort, there were no significant differences in the risks of obesity of the birth cohorts after 1951-1955 (P>0.05). But the risk of obesity from 1946-1950 birth cohort to 1931-1935 birth cohort was gradual y significant since the 2000;obesity risks of those born in the different times were different;rural area wil be the key area than 45 years old.

Objective To discuss nursing care of patients underwent corneal Descemet stripping endothelial keratoplasty to treat bullous keratopathy. Methods 22 patients who underwent corneal Descemet stripping endothelial keratoplasty to treat bullous keratopathy accepted strengthened psychological care, perfect preoperatively preparation, to sleep in face-up supine position, meanwhile complications were observed and treated carefully. Results 22 patients could persist in sleeping in face-up position for 4days postoperatively. Graft shift occurred in two cases. And after gas reinjection the grafts adhered very well. Intraocular pressure rose in 7 cases .After timely treated these 7 patients were cured and discharged. Conclusions Careful preoperative evaluation and preparation could reduce postoperative complication. Strict measures to keep patients sleeping in face-up supine position, timely observation and treatment of complications should be important to ensure the success of the operation.

Objective To study wether dezocine (DZ) can attenuate neuropathic pain in rats and to investigate its mechanism.Methods Neuropathic pain was induced by tight ligation of the left L5 spinal nerve of the male SD rats. 24 Spinal nerve ligation (SNL) model rats were randomly divided into three groups:the first group (saline group, n=8), rats were given intraperitoneal injection of 1ml of saline 14 days after SNL surgery; the second group (dezocine group, n=8), rats were given intraperitoneal injection of 5mg/kg of dezocine in 1ml saline 14 days after SNL surgery;the third group (morphine group,n=8),rats were given intraperitoneal injection of 5 mg/kg of morphine 14 days after SNL surgery. Results Intraperitoneal injection dezocine and morphine had significant analgesic effect in neuropathic pain rats. This analgesic effect lasted for 4-6 hours.Continuous injection of morphine for 3 days produced significant tolerance, while continuous injection of dezocine for 7 days still had significant analgesic effect on neuropathic pain. Conclusion Intraperitoneal injection of dezocine and morphine can attenuate SNL neuropathic pain, continuous injection of morphine for 3 days can produce significant tolerance, while continuous injection of dezocine for 7 days still has significant analgesic effect on neuropathic pain.

Objective To evaluate the efficacy of transanal local resection for early low rectal cancer. Methods The clinical data of 72 patients with early low rectal cancer who had been admitted to Union Hospital of Fujian Medical University from January 1997 to April 2009 were retrospectively analyzed. Patients were divided into 2 groups, and they received transanal local resection (group A, n = 31) and radical resection (group B, n =41), respectively. Postoperative local recurrence and distal metastasis were analyzed by Fisher exact test, the survival was analyzed by life table method. The difference in survival between the 2 groups was analyzed by Wilcoxon test. Results Three patients were found to have surgical complications in group B, while no complication was detected in group A. The local recurrence was 6% (2/31) in group A and 0 (0/41) in group B (P < 0.05); the distal metastasis was 6% (2/31) in group A and 2% (1/41) in group B, with no significance difference between the 2 groups (χ~2 =0. 062, P >0.05). The 3- and 5-year survival rates were 93%, 87% in group A, and 95%, 91% in group B, with no significant difference between the 2 groups (χ~2 =0.099, 0.144, P >0.05). Conclusions The prognosis of patients with early low rectal cancer who received transanal local resection is similar to those who received radical resection. Transanal local resection is safe and feasible when the indications are strictly followed.

Objective To summarize the nursing points of the patients with refractory glaucoma un-dergone ahmed valve insertion with donor human sclera patch.Methods The nursing points of 102 con-secutive refractory glaucoma cases who underwent ahmed glaucoma valve implant insertion with donor hu-man sclera patch placed over the tube were studied retrospectively.Results 100 (98.04%) glaucoma-tous valves kept on being unobstructed postoperatively.Complications occurred in some patients including hyphema(5 cases), tube exposure (1 cases), shallow anterior chamber(16 cases), hypertension accom-panied with eye pain, headache, and corneal edema early after surgery (14 cases received paracentisis within 3 days), tubes obstructed by the vitrous needed vitrectomy (3 cases), obstructed by silicon oi1(2 cases).Conclusions Paying attention to psychological nursing and health instruction preoperatively, close observation after surgery, and correct instructions for the out- patients, are the key points in the reha-bilitation process after ahmed valve insertion with donor human sclera patch.