Objective To assess the violence risk levels of the community patients with severe mental illness and Analyze the associated factors.Methods A working group composed of psychiatrists,community police,FIMITIC staff,community physicians,neighborhood or village committee members was established.501 community patients with severe mental illness were evaluated with Violence Risk Assessment Scale by working group.While the data of the general demograpbic,medical history and violence history was investigated.Logistic regression analysis was used to explore the factors associated with violence.Results In 501 patients,risk levels of grade 0:124cases (24.8％),grade 1:165 cases (32.9％),grade 2:118 cases (23.5％),grade 3:59 cases (11.8％),grade 4:28 cases (5.6％) and grade 5:7 cases (1.4％).Logistic regression analysis showed four significant risk factors,which were schizophrenia (OR 4.139),no taking medicine (OR 6.883),unstable condition (OR 234.534) the number of previous violence(OR 3.457) (P ＜ 0.05).Logistic regression equation:Logit (severe violence) =-8.511 + 1.420 (schizophrenia) + 1.929 (no taking medicine) + 5.458 (unstable condition) +1.241 (the number of previous violence).Conclusion The results of violence risk assessment and risk factors have directive significance for management of community patients with severe mental illness.

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0.05).At the end of the experiment the doses of mixed insulin group were higher than that of glargine group significantly(P

Objective:To evaluate the influence of delayed application of mixed self-etching bonding agent on the tensile bond strength.Methods:Two "two-bottle,one-step" self-etching adhesives,Adper Prompt and Xeno III,were evaluated.The occlusal enamel of human third molar was cut out and the exposed dentin surface was divided in two halves by a 1 mm deep groove in the labial-lingual orientation to allow for evaluation of immediate application and delayed application(0.5,1,2,3,4,5 and 6 h) of mixed self-etching bonding agent in the same specimen,followed by composite resin built-up on the adhesive.The bonded specimens were sectioned into beams of approximately 0.9 mm2 and subsequently subjected to micro-tensile bond strength testing at a crosshead speed of 1.0 mm/min.Results:The specimens bonded with the Adper Prompt within 5 h after mixing showed no different and that with the Xeno III within 3 h after mixing showed no different.Conclusion:The effective period for "two-bottle,one-step" self-etching adhesives after mixing is limited.Different bonding agents have different effective periods.

Objective To study the chemical constituents from the wing twigs of Euonymus alatus. Methods Compounds were isolated and purified repeatedly on silica gel, Sephadex LH-20, open RP-C18 column chromatographies and PTLC, and their chemical structures were elucidated by their physicochemical properties and spectral data, such as 1H-NMR, 13C-NMR, and EI-MS. Results Nine compounds were obtained and identified as: epifriedelinol (Ⅰ), stigmast-4-en-3-one (Ⅱ), 6?-hydroxy-stigmast-4-en-3-one (Ⅲ), ?-sitosterol (Ⅳ), methyl 2, 4-dihydroxy-3, 6-dimethyl benzoate (Ⅴ), methyl 2, 4-dihydroxy-6-methyl benzoate (Ⅵ), 7-methoxy-4-methylphthalide (Ⅶ), vanillin (Ⅷ), n-octacosanol (Ⅸ). Conclusion Compound Ⅶ is first reported as a natural product. Compounds Ⅴ-Ⅷ are reported from plants of Euonymus L. for the first time.

Objective: To study the effects of the melt casting and the sintering methods on the machinability and other characteristics of K_2O-MgO-MgF_2-SiO_2 glass-ceramics. Methods: X-ray diffraction(XRD) and scanning electron microscopy (SEM)were used to examine the main crystal and the microstructure of the glass ceramics of the same composition which were made by melt casting and sintering methods, respectively. The transmissivity, flexural strength and brittleness index were observed respectively. Results: The glass ceramics made by two different methods have the same crystal, KMg_(2.5)Si_4O_(10)F_2, while the specimen prepared by the sintering method had lower transmissivity,better machinability and mechanical properties due to the higher mica volume percent. Conclusion; For production of fluoro-mica glass ceramics, sintering method has superiority compared to melt-casting method.

Objective Toll-like receptor ( TLR) gene family is the most important pathogen recognition receptor and animal experiment have found TLR4 mice is inclined to infect aspergillosis ( IA) .The study was to investigate the variation of TLR4 gene in Chinese Han nationality and its relation with the susceptibility of IA. Methods 25 patients diagnosed with proven IA from June 2011 to December 2012 in our hospital were enrolled, among which 17 were males.Their average age was 52.4 ±12.3, and 12 pa-tients had underlying diseases, the others had no underlying diseases.The control group consisted of 103 normal persons, among which 70 were males.Their average age was 56.0 ±17.2.All of the subjects were Chinese Han population.DNA was extracted from periph-eral blood.Polymerase chain reaction ( PCR) was applied to amplify the coding sequence of TLR4 gene followed by sequencing.The sequencing result was compared with TLR4 coding sequence in NCBI GenBank along with the analysis on amino acid change caused by genetic mutation and its effect on protein function.Comparison analysis was made on genetic mutation rate between IA group and con-trol group. Results Two missense mutations,TLR4 219 C>G and 1420 C>T, were identified in TLR4.The prediction result of protein structure showed 219 C>G resulted in the change of functional area for TLR4 to recognize pathogen and 1420 C>T caused no change in domains.The variation rate of TLR4 in IA group was 8%( 2/25 ) and both the patients had no underlying diseases. No mutation has been founded in control group and the difference between two groups was of statistical significance (P=0.037). Conclusion Two missense mutations (219 C>G and 1420 C>T) have been detec-ted in encoding area of TLR4 gene of IA patients.Variation in the cod-ing region of TLR4 gene may increase the susceptibility to IA.

Objectives To investigate the bacterial pathogens and their antibiotic resistance in children with pneumonia in Suzhou area. Methods Sputum specimens of 8179 hospitalized children with pneumonia from October 2005 to September 2011 were collected by sterile negative pressure aspirator and then cultured. The incidence of positive bacteria was tested by Kirby-Bauer method and broth dilution method, and their antibiotics resistances were analyzed. Results A total of 2391 of 8179 children were infected with positive rate of 29.23%. A total of 2552 strains of bacteria were detected with Streptococcus pneumoniae (42.40%), Haemophilus inlfuenzae (14.81%), Moraxelle catarrhalis (11.01%), Staphylococcus aureus (8.50%) and Escherichia coli (5.02%) as the most common strains. The infection rate of different bacterium was related to age, season and the type of pneumonia (all P<0.001). The tendency of antibiotic resistances in 2 140 bacterial isolates was found increased by the susceptibility test. Conclusions Streptococcus pneumonia and Haemophilus inlfuenzae are the most common bacterial pathogens in children with pneumonia in Suzhou area. The positive rate of different bacterium was related to age, season and the type of pneumonia. Attention must be paid to the rational use of antibiotics to reduce drug resistance.

Objective To observe efifcacy and safety of catheter ablation for atrial ifbrillation (AF) occurring after surgical valve replacement in patients with rheumatic heart disease (RHD). Methods A total of 23 RHD patients with atrial ifbrillation after surgical valve replacement were enrolled in this study from 2008 to 2013. The clinical characteristics, ablation strategies and successful rate were investigated. Results All the cases included 8 males and 15 females (age, 51.0 ± 9.2 years). Valves replaced were isolated mitral valves (13/23, 56.5%) and multiple valves (10/23, 43.5%). Postoperative AF after cardiac surgery was paroxysmal in 14 patients (60.9%) and nonparoxysmal in 9 cases. Nine patients (39.1%) was in sinus rhythm before cardiac surgery, 4 in paroxysmal AF and 10 in non-paroxysmal AF. The mean interval between the catheter ablation AF and the surgical intervention was (6.9±5.8) years. The postoperative AF duration was (3.1±3.2) years, left and right atrial diameters were (44.1±5.9) mm and (48.1±9.0) mm respectively, left ventricular ejection fraction was 64.0%±8.3%, the mean ablation procedure duration was (156.8±46.6) min, and lfuoroscopy exposure averaged (27.3±11.2) min. Standard pulmonary vein isolation was performed in all cases by using ipsilateral circumferential ablation technique. Additional ablation, including complex fractionated atrial electrograms, mitral and tricuspid isthmus, and left atrial roof, was applied in most of the cases. After a mean follow-up of (29.7±21.2) months (median, 24 months), 60.9%of the patients remained free of AF, 1 died, and 2 lost to follow-up. Conclusions Catheter ablation for AF is effective and safe in patients with RHD after surgical valve replacement. Stepwise ablation strategy may be better for these patients.

A fusion gene called Ig-BDNF, in which brain-derived neurotrophic factor cDNA fused to the 3' end of signal peptide of Ig coding sequence, was constructed by PCR, digested and subcloned into shuttle plasmid pSNAV to obtain a recombinant plasmid pSNAV-Ig-BDNF. Then the plasmid encoding fusion protein was transfected into 293 cell lines and the stably transfected clones were selected with neomycin. AAV1 containing Ig-BDNF fusion gene vectors were obtained by super-infection by Herpes virus. The resultant adeno-associated virus vectors AAV-Ig-BDNF were confirmed by PCR, Western blotting and a sandwich enzyme-linked immunosorbent assay (ELISA) after infection of 293 cell lines. The results indicated that AAV-Ig-BDNF contained the target gene, and infected cells and produced the fusion protein into the supernatant. The content of BDNF in medium per 5x104 cells over a 24 h incubation period reached 1000 pg/mL. With the help of non-replicative adenovirus during AAV-Ig-BDNF infection, the expression of BDNF increased 7-8 fold, and the enhancement of BDNF gene expression was observed in a concentration-dependent manner. These results suggested that a functional AAV-Ig-BDNF was successfully constructed and it offers basis for further study for gene therapy of neural degeneration diseases.
Brain-Derived Neurotrophic Factor ; biosynthesis ; genetics ; Cell Line ; Dependovirus ; genetics ; metabolism ; Genetic Vectors ; genetics ; Humans ; Recombinant Fusion Proteins ; biosynthesis ; genetics ; Recombination, Genetic ; Simplexvirus ; genetics ; Transfection