A Review] Endothelial cells produce both superoxide and nitric oxide. Nitric oxide and superox- ide are known to react rapidly to form the stable peroxynitrite anion. Peroxynitrite mediates the oxidation of protein, lipid, deoxyribose and inhibits mitochondrial electron transport. Peroxynitrite may break DNA strands and activate poly(ADP - ribose) syntheatase. If the reaction is excessive, it results in a depletion of intracelular NAD+ and ATP. There is ultimately cell death.

Objective:To explore the diagnostic value of color soppler flow imaging(CDFI) be applied to pseudoaneurysms in different parts of the body.Methods: Retrospectively analyzed clinical data of 29 pseudoaneurysms patients who were diagnosed by Color Doppler Flow Imaging during january 2012-october 2013, to discuss the features of two-dimensional echocardiography and CDFI signal,all the diagnostic results were compared to the operation results.Results: The biggest lump among 29 patients is 6.3 cm×11.8 cm×12.5 cm, located in femoral artery, the smallest one is 2.3 cm×2.8 cm×0.5 cm, located in carotid artery,all the patients were confirmed by operations, The coincident diagnostic rate was 100%, 39 Pseudoaneurysms had been found in total, among these, the number of patients have single Pseudoaneurysms were 22, about 75.86%, 4 patients have one more pseudoaneurysms, about 13.79%, 3 patients have two more pseudoaneurysms,about 10.34%. 23 breachs have been found by two-dimensional echocardiography, and 6 breachs haven’t been found, but all the breachs have been found by CDFI. Back and forth movement flow pattern was the main diagnostic sign.Conclusion: Color doppler flow Imaging is safe and noninvasive,and have high diagnostic value in diagnosing pseudoaneurysms, it is the preferred method in diagnosis.

Objectives To study the feasibility of total mesorectal excision (TME) by laparoscopy for rectal cancer.Methods The mesorectum in the resected samples and the number of lymph nodes resected were compared between laparoscopic (n=20) and open surgery group (n=20).Results ①The mesorecum of resected specimen was all complete in two groups. ②The lymph node yield (N 1) in laparoscopic and open group was 8.5?3.6 vs. 10.6?6.2(P=0.334), respectively. That in N 2 was 3.7?2.2 vs. 2.4?2.1(P=0.328). That in N 3 was 2.4?1.7 vs. 2.0?1.8(P=0.590).③The distal margin was all tumor-free in the two groups. ConclusionLaparoscopy is capable of performing TME in patients with rectal carcinoma undergoing radical resection.

Objective Studying the related factors of left ventricular hypertrophy for hypertensive disease, to provide the basis for clinical treatment. HZ Methods 500 hypertensive disease patients were performed dynamic monitoring of blood pressure and UCG. Then 130 patients with left ventricular hypertrophy were compared the dynamic blood pressure, age, sex and diseases course with 130 patients who had not left ventricular hypertrophy. Results (1) Systolic pressure, the load and the night descent rate of systolic pressure were significantly related to left ventricular hypertrophy, there was obviously difference in two groups. To the patients with systolic pressure and diastolic pressure were all increased, the incidence rate of left ventricular hypertrophy was reduced. (2) The age of left ventricular hypertrophy group was higher than no left ventricular hypertrophy group. (3) The female's incidence rate of hypertensive disease in left ventricular hypertrophy group was higher than no left ventricular hypertrophy group. (4) The course of diseases in left ventricular hypertrophy group was extended than no left ventricular hypertrophy group. The incidence rate of left ventricular hypertrophy was obviously higher in the patients whose blood pressure was increased in the daytime. Conclusion (1) The pulse pressure is a important forecast factor in numerous risk factor. (2) The advanced age is a risk factor to hypertensive disease. (3) The female hypertensive disease patients take place left ventricular hypertrophy is easier than the male. (4) Left ventricular hypertrophy is significantly related to hypertensive disease course, and isn't significantly related to blood pressure's durative. The clinic must think highly of observing and evaluating the pulse pressure, the long disease course of old female patients. There is important clinical significance to reinforce monitoring the risk factor of left ventricular hypertrophy, and to perform pertinent treatment and prevention for the high risk patients.

Objective:To investigate the stress distribution surrounding an implant under different thickness of cancellous bone and cortical bone,and to analyze the influence of thickness ratio and total thickness of bone tissues on the reliability of an implant.Methods:By using the commercial finite element method software Abaqus, a simplified three-dimensional model of a jawbone consisting of a cancellous bone,a cortical bone,an implant,and a ceramic crown was constructed,and then the computation was performed.Under the condition that the system was loaded by lateral and normal stresses, the influence of thickness ratio and total thickness of cancellous bone and cortical bone in the stress distribution surrounding the implant was studied,where the thickness ratios were 3∶1, 2∶1, 1∶1, 1∶2, and 1∶3;the total thickness were 0.5, 1.0, 2.0, 3.0 and 4.0 mm, respectively. Results:The maximum stresses on the cortical bone,the cancellous bone as well as the implant were all found to decrease with the increasing of the total thickness of cortical and cancellous bones,with a higher decreasing rate in the range between 0.5-2.0 mm and a lower decreasing rate between 2.0-4.0 mm. More importantly, the maximum value of stress in the cortical bone within the neck region of the implant was observed to increase dramatically via reducing the total thickness below 2 mm, while it was increased insignificantly when the total thickness was above 2.0 mm. Conclusion:The thickness ratio and the total thickness of cancellous bone and cortical bone have strong influence in the stress distribution surrounding the implant.In dental implantation surgery, the total thickness of cancellous bone and cortical bone should be at least 2 mm, and therefore 2 mm is an optimal value.

OBJECTIVE： Four different one－ week triple therapeutic schemes to eradicate Helicobacter pylori were compared by pharmacoeconomic analysis to provide a scientific method for rational assignment of our limited medical fund.METHODS： According to literature reports,892 cases of duodenal ulcer and gastritis with Helicobacter pylori were selected.These patients were randomly divided into four groups which received different one－ week triple therapeutic schemes： OMC(Omeprazole＋ Metronidazole＋ Clarithromycin),OFC(Omeprazole＋ Furazolidone＋ Clarithromycin),OFA(Omeprazole＋ Furazolidone＋ Amoxicillin)and BFC(Colloidal bismuth subcitrate＋ Furazolidone＋ Clarithromycin).The schemes were evaluated with pharmacoeconomic cost－ effectiveness analysis.RESULTS： The cost－ effectiveness ratios of OMC,OFC,OFA,BFC for gastritis or duodenal ulcer were 16.19/17.33,17.30/13.93,11.40/10.56,11.17/10.75 respectively.They changed to 16.36/17.51,17.49/14.08,8.15/7.55,11.66/11.20 by sensitivity analysis.CONCLUSION： OFA is the most effective and inexpensive one to eradicate Hp.

A method for the rapid enantioseparation of five β-blocker drugs, including alprenolol, propranolol), mexiletine, metoprolol and pindolol was developed by a 5-cm short column packed with perphenylcarbamoylated β-cyclodextrin(CD) chiral stationary phase by HPLC. The results showed that except for alprenolol), the other 4 β-blocker drugs were completely separated using ACN-0.1% thriethylammonium acetate(TEAA), 40∶ 60, V/V, pH=4.0) as mobile phase. The 5-cm short CD-based column exhibited rapid separation ability to the above β-blocker drugs within 5 min, which indicated that the separation has high efficiency. According to the chemical structures of the β-blockers and their chromatographic behavior, related separation mechanisms were also discussed. The proposed method was rapid, effective and repeated.

Objective To investigate the value of multi-slice CT in the diagnosis of gastrointestinal stromal tumor. Method CT image of 15 cases of GIST confirmed by surgical pathology were analyzed retrospectively. Re-sults The tumors originated from the stomach(n = 8), duodenum (n = 1),jejunum and ileum (n = 3), colon(n = 1), mesentery(n = 2). The main display of CT were hemorrhage,necresis and cystic change within the tumor. The en-hancement of parenchyma was clear. The enhancement of venous phase was more clear than that of arterial phase. Conclusion MSCT is a valuable examination for GIST. MSCT can precisely display the location, shape, size and en-hancement of GIST. MSCT can be worth the locating,qualitative and staging diagnosis.

The incidence of venous thromboembolism after spinal cord injury is high, and its diagnosis and prevention received much attention in clinical research in recent years. Because of the special characteristics, the prevention of venous thrombosis should be combined with basic prevention, physical prevention and drug prevention. According to the different situations of venous thromboembolism in patients with spinal cord injury, the prevention methods need to adjust to carry out multidisciplinary comprehensive treatment.

Objective To investigate the expression and clinical significance of RegⅣand EGFR,PI3K proteins in gastric adenocarcinoma .Methods S-P immunohistochemistry was used to detect the expression of RegⅣand EGFR,PI3K proteins in pathological tissues of 73 cases with gastric adenocarcinoma and tumor -adja-cent normal gastric tissues .Results The positive expression rates of RegⅣand EGFR,PI3K proteins in 73 cases with gastric adenocarcinoma tissue were 50.7%(37/73),56.2%(41/73) and 69.9%(51/73) respectively, which were significantly different from the positive expression rates of tumor -adjacent normal gastric tissues ,be-ing 20.5%(15/73),19.2%(14/73),and 21.9%(16/73),respectively(P<0.05).The expression of RegⅣprotein was significantly correlated to differentiation degree ( P<0.05 ) and the expression of EGFR protein was significantly correlated to infiltrative depth,TNM stage,and lymph node metastasis(P<0.05).The expression of PI3K protein was significantly correlated to differentiation degree ,infiltrative depth,TNM stage and lymph node metastasis(P<0.05).There was positive correlation between the expressions of RegⅣ/EGFR,RegⅣ/PI3K and EGFR/PI3K proteins in gastric adenocarcinoma and the values of Spearman coefficient correlation were 0.325, 0.403 and 0.384,respectively(P<0.05).Conclusion RegⅣmay play an important role in gastric adenocarci-noma genesis and progression by activating EGFR /PI3K/Akt signaling pathway .