Graft versus host disease is the most common complication of allogeneic hematopoietic stem cell transplantation.It includes two different types,i.e.acute and chronic types.Acute graft versus host disease (aGVHD) is the immune response of T cells from the donor to unmatched major histocompatibility complex (MHC) from the host.It has unique pathogenesis and pathological changes and complex clinical manifestations with multiple organs involved.This paper reviewed the progress from home and abroad in diagnosis and treatment of acute graft versus host disease.

The changes of plasma Fn content in rabbits of hemorrhagic and endotoxic shock has been assayed by means of single and double immunodiffusion tests. The results showed that Fn level significantly declined at 30 minutes after hemorrhagic shock and remained at low level with development of shock. But in the rabbitns transfused at 30 minutes after shock Fn level turned to normal as blood pressure increased. Fn content was significantly elevated at 30 minutes and significantly decreased at 60 minutes after endotoxic shock. The possible pathophysiologic and clinical significance of these changes was disscussed.

The pathogenesis and clinical manifestation of histiocytosis are veriant, and misdiagnosis is relatively high. We reviewed a total of cases of this disease and related literature, and the possibility pathogenesis and its clinical features. were discussed.

Objective To explore the clinical diagnosis and surgical treatment of inflammatory pseudotumor of the lung. Methods From January 2002 to December 2008,the clinical data of 50 cases with pulmonary inflammatory pseudotumor were retospectively analyzed. Result 18 cases of preoperative diagnosis, the four cases of lobectomy, pulmonary wedge resection in 13 cases, and the rest are mass surgery, intraoperative tumor mostly see substantive, tumor visceral pleural surface without sag and shrinkage, tumor palpation There are hard and soft white felt, with incomplete tumor capsule, easily stripped. Resection of the pathological specimens were confirmed to be inflammatory pseudotumor, no operative complications and death in patients with follow-up 1～8 years, both curvived, without recurrence. Conclusion Inflammatory pseudotumor of their clinical performance and imaging characteristics of the lack of change in the low rate of preoperative diagnosis, surgical treatment of the main diagnostic, intraoperative frozen biopsy to determine pathology surgery, surgical approach to the limitations of pneumonectomy or lobectomy excision.

Objective: To make an English Learning Anxiety Scale (ELAS) based on the Foreign Language Classroom Anxiety Scale (FLCAS) and to do a psychometric test of the ELAS. Method: After translation of the FLCAS into Chinese and did item adaptation to form an ELAS. It was administered to 669 college students with major field other than English. Result: The ELAS was composed of 23 items in four subscales: nervousness , worry, apprehension of being questioned, and sense of anxiety.Factor loadings in the exploratory factor analysis were: A1, 0.46-0.70; A2, 0.53-0.73; A3, 0.55-0.70, A4,0.50-0.67. 48.95% of the total variance could explained. The Cronnbach?is 0.70-0.89, split-half reliability(S-B), 0.71-0.88. ?2/df, RMSEA, GFI, AGFI, NFI, NNFI, CFI, IFI of the goodness of fit indices of confirmatory factor analysis were 2.40, 0.05, 0.92, 0.90, 0.85, 0.89, 0.90, 0.90. The correlation coefficient between ELAS and CET4 score is-0.25~0.64. The correlation coefficient between ELAS and the subscales was 0.69~0.80. The correlation coefficient between the subscales was 0.45~0.63. Conclusion: ELAS has good reliability and validity, could be an effective research instrument in studying anxiety of Chinese college students with major field other than English.

Objective To prepare calcium alginate-chitosan intragastric floating beads and investigate the influences of various factors on the floating characteristics of beads.Methods Sodium alginate solution added with frother was slowly dripped into chitosan-calcium chloride solution added with acetic acid,calcium alginate-ehitosan intragastric floating beads were obtained after drying.The effects of frother,addition amount of acetic acid,the concentration of alginate solution,chitosan and calcium chloride solution on the floating characteristics of beads were investigated.Results Frother and addition amount of acetic acid were the main influence factors on the floating characteristics,and the concentrations of alginate solution, chitosan and calcium chloride solution also had some influences.Conclusion The calcium alginate-chitosan intragastrie floating beads with favorable floatability could be achieved through adjusting the formula,it laid a foundation for the furether development of the preparations in intragastric floating preparations.

The ghrompspine fr.agile .sites and micronucleus expression of peripheral lymphocytes were studied in 23 cases with lung cancer 23 cases with uterus cancer 13 cases with ovary cancer and 20 control subjects, using TC199 medium containing 5% bovine serum. The results showed that the chromosome aberration and micronucleus rates of the three group patients were significantly higher than that of the control group, but differences between the three group patients were not statistically significant

BACKGROUND:It is confirmed that the onset of osteoarthritis is closely related to inflammatory cytokines. OBJECTIVE: To investigate the levels of interleukin-6 and matrix metaloproteinase-3 in the synovial fluid of patients with osteoarthritis and their correlation with the severity of osteoarthritis. METHODS: Synovial fluid specimens of 78 patients were harvested to detect the levels of interleukin-6 and matrix metaloproteinase-3 using ELISA assay. The severity of osteoarthritis was estimated by the Hospital for Special Surgery (HSS) score. Pearson correlation analysis was adopted to explore the pairwise correlations between interleukin-6, matrix metaloproteinase-3 and HSS score. RESULTS AND CONCLUSION:The levels of interleukin-6 and matrix metaloproteinase-3 in the synovial fluid were (13.1±5.7) and (989.3±429.5) μg/L, respectively; the HSS score was (56.4±12.0) points. Pearson correlation analysis showed that the expression levels of interleukin-6 and matrix metaloproteinase-3 had a significant positive correlation (r=0.70,P< 0.001); the levels of interleukin-6 and matrix metaloproteinase-3 had a negative correlation with the HSS scores (r=-0.70,r=-0.75,P < 0.001). Experimental findings show that there is a positive correlation between interleukin-6 and matrix metaloproteinase-3 in the synovial fluids, and these two cytokines also have a positive correlation with the severity of osteoarthritis, indicating that to measure interleukin-6 and matrix metaloproteinase-3 levels in the synovial fluids of osteoarthritis patients is important for early diagnosis and treatment of osteoarthritis.

The phospholipid content in BALL markedly increased (P0.01) in septic rats induced by cecal ligation plus punctures as compared with non-septic control. Meanwhile, the lamellar inclusion bodies in type Ⅱ alveolar epithelial cells were empty observed in electro-microscopic examination. Authors suggest that the changes mentioned above is a compensatory response related to stress in early stage of sepsis.

Apolipoprotein E (ApoE) was purified from human serum and used as the antigen to immunize BALB/C mice. The splenic cells of the mice fused with NS-1 mouse myeloma cells. Three hybridoma cell lines(E3C3, E6C3 and E4C2)secreting anti-human ApoE McAb were established. The immunological property of the McAb was studied. The ascites fluids titer were 8?10~(-5)2?10~(-6). The McAbs did not cross react with the other apolipoprotins. ApoE was purified by immunoaffinity chromatography prepared by using the ApoE McAb. McAbs recognized two distinct respective epitopcs on ApoE. The subclass of immunoglabulin of three McAbs were IgG_1. A double McAb sandwich EL1SA was developed to evaluate ApoE levels in normal and hyperlipidemia human serum.