Objective To evaluate the clinical prevention and cure efficiency of panax notoginosides(PNS) in patients with chronic renal failure.Methods 60 patients with chronic renal failure were selected and randomly divided into the control group and the observation group,with 30 cases in each group by random number table.The patients in the control group underwent regular maintenance hemodialysis(MHD),while the patients in the observa-tion group were given PNS glucose injection on the base of the control group for two months treatment.Comparison was made between the two groups in the aspect of liver filament four items,renal function,blood lipids and the levels of TGF-β1 ,TIMP-1 expression for renal fibrosis.Results After two months treatment,the liver filament four items (t=3.94-5.62,all P<0.05),renal function (t=4.15,4.29,all P<0.05),blood lipids (t=3.83 -5.47,all P<0.05)and the level of TGF-β1,TIMP-1 were all significantly decreased in the observation group,while HA, blood lipids and the level of TGF-β1(t=5.19,P<0.01),TIMP-1(t=4.16,P<0.05)were also decreased in the control group.All the indexes of the observation group were much significant than those of the control group.Total effective rate of the observation group was 83.33%,which was significantly higher than 66.67% of the control group (χ2 =5.16,P<0.05).There was no adverse reaction in the observation group.Conclusion PNS could improve renal fibrosis by lowering the level of TGF-β1 and TIMP-1 ,and could be used as conventional auxiliary drug for patients with chronic renal failure.

ObjectiveTo analyse the clinical characteristics of elderly patients with permanent cardiac pacemaker implanted. MethodsTo retrospectively analyze 41 elderly patients( ≥80 years) with permanent pacemakers implantation. Observing the clinical symptoms, bradyarrhythmia category, pacing mode, intraoperative pacing parameters,intraoperative and postoperative complications. ResultsAll patients were operated successfully. Pacing modes were,VVI 29 cases,VVIR 2 cases,DDD 9 cases and DDDR 1 case. Ventricular electrode located in right ventricular apical pacing. Atrial electrode located right heart ear. During the operateing, severe complications did not happen such as heart perforation, malignant arrhythmia. One case of postoperative dislocation electrode and one case of pocket hematocele were observed. After treatment, they were cured, no infection and pacemaker syndrome happened. 6 patients died during follow-up, postoperative from two months to 6 years. ConclusionPacing therapy in elderly patients is good. Surgical risk was not increased.

Objective To explore the effects oflosartan on brain natriuretic peptide(BNP)and C-reactive protein(CLRP)in diabetic cardiomyopathy patients. Methods Sixty diabetics were divided into diabetic control group(30 cases)and diabetic cardiomyopathy group(30 cases), and the levels of BNP and CRP in plasma were determined. Then, diabetic cardiomyopathy group were divided into conventional treatment group(15 cases)and losartan treatment group(15 cases)by random digits table to take corresponding treatment for 12 weeks. The changes of cardiac function, the levels of BNP and CRP in plasma were detected and compared. Results The levels of BNP and CRP in diabetic cardiomyopathy group [(331.27 ±65.64)ng/L,(26.10±10.13)mg/L]were significantly higher than those in diabetic control group[(76.09±39.14)ng/L,(7.03±2.71)mg/L](P＜0.01). After treatment 12 weeks, the levels of BNP and CRP were significantly lower, left ventricular ejection fraction was significantly higher and left ventricular end-diastolic diameter was significantly lower in losartan treatment group than those in conventional treatment group(P＜0.05).Conclusions The increasing levels of BNP and CRP can reflect clinical severity of diabetic cardiomyopathy.Losartan can decrease the levels of BNP and CRP, and improve heart function, and it has better effective on diabetic cardiomyopathy.

Objective To probe into the changes of rats' plasma SOD,MDA,NO and rectal temperature at moist heat and wound stress in order to build a basis for research of enhancing resistance to stress resporise in human. Methods 56 moist beat and wound rats were randomly divided into the control group and the nutrition group with 28 rats in each group.then each group was subsequently divided into 4 period oftime.All the backs of rats were scalded in superficial degree Ⅱ by 99℃water and exposed to the same conditions:temperature at(37.O±0.5)℃and relative humidity at(65±5)%.The control group' was imbued with double-distilled water for one week,while in the nutrition group,compound nutrient was imbued for one week.SOD,MDA,NO and rectal temperature at 4 time points were compared between the two groups.Results The differences of rectal temperature,NO and SOD changes between the control group and the nutrition group were remarkably significant.Conclusions Early nutrition is effective in reducing stress,decreasing organ injury caused by NO,SOD and MDA level and alleviated the damage of body.

Objective To explore the risk factors and investigate the intracranial vascular characteristics in elderly patients with WSI. Methods According to imaging features of DWI,56 cases of WSI were divided into CWSI,IWSI and MWSI group. Distribution of intracranial vascular le?sions in different groups was compared. The degree of middle cerebral artery(MCA)and internal carotid artery(ICA)stenosis in different types of WSI was investigated. Results Among 56 cases,85.7%of them had a history of hypertension,53.6%had diabetes,62.5%had hyperlipoidemia, 51.8%had coronary heart disease(CHD),51.8%had smoking,and 73.2%of them had two or more risk factors. Totally 11 cases(19.6%)of them were cortical watershed infarction(CWSI),13(23.2%)of them were interior watershed infarction(IWSI),and 32(57.1%)of them were mixed cerebral watershed infarction(MWSI). Among all the patients,the incidence of MCA stenosis(37.5%)and ICA stenosis(53.6%)were signifi?cantly higher than the other intracranial vessels(χ2=37.188,P<0.001). The incidence of MCA stenosis in IWSI group was significant higher than CWSI and MWSI group(χ2=12.00,P<0.01). The incidence of ICA stenosis in MWSI group was significantly higher than CWSI group and IWSI group(χ2=11.10,P<0.01). Among all the patients,17 of them had severe stenosis or occlusion in MCA(30.4%),and 22 of them had severe ste?nosis or occlusion in ICA(39.3%). The incidence of severe stenosis or occlusion in MCA(58.8%)was significant higher in IWSI group(χ2=7.588,P<0.05)and those in ICA was significantly higher in MWSI group(χ2=7.091,P<0.05). Conclusion MWSI is more common in elderly patients with WSI. Most of the patients have more than one risk factors such as hypertension,diabetes,hyperlipoidemia,CHD and smoking. MCA and ICA lesions are more common than other intracranial vessels in elderly patients. IWSI is closely related with severe stenosis and occlusion of MCA,while MWSI is closely related with severe stenosis and occlusion of ICA.

ObjectiveTo observe the effects of enriched environment on depressive-like behaviors induced by chronic stress in rats.MethodsAdult rats were randomly divided into stress group and the control group,then after 21 days of stress,rats were raised in an enrich environment (EE) or standard environment (SE)for 28 days.Open field test,forced swimming test and sucrose preference test were used to detect depressive-like behavior in rats.Results ( 1 ) Open field test showed that grid crossing,standing and rearing in stress ( no gap)rats were significantly reduced ( 13.88 ±4.38 vs 45.00 ± 10.19,9.13 ±2.54 vs 16.38 ±4.11 and 4.78 ± 1.39vs 10.51 ± 2.52 ; n =8 ; P＜ 0.01 respectively) ; but grid crossing and standing in stress + EE rats were significantly increased than stress + SE rats (41.61 ± 10.53 vs 26.25 ± 6.18 and 16.79 ± 3.49 vs 11.25 ± 3.12 ; n =8 ; P＜0.01 and P＜0.05 respectively).(2) Sucrose preference tests showed that sucrose consumption and sucrose preference％ in stress ( no gap) rats were significantly decreased than those in the control group ( 5.22 ± 0.94 vs 10.61 ±2.59 and (49.11 ±6.77)％ vs(63.38 ±8.36)％ ; n=8; P＜0.01 respectively).(3) Forced swimming test showed that immobility time was increased in stress ( no gap) rats ( ( 19.5 ± 5.43 ) s vs ( 12.75 ± 3.9 ) s; n =8 ; P ＜ 0.05 ) and was restored by EE compared to SE rats ( ( 10.25 ± 3.57) s vs ( 17.75 ± 5.45 ) s; n =8 ; P ＜ 0.05).ConclusionChronic stress can reduce depressive-like behaviors,and EE can restore depressive-like behaviors induced by chronic stress.

Objective To investigate the prevalence and clinical characteristics of thyroid dysfunction induced by IFNα therapy in patients with chronic hepatitis.Methods Thyroid function of 310 patients with chronic hepatitis receiving IFNα therapy were evaluated.Results Serum free tri-iodothymnine (F13),free thyroxine(FT4)and sensitive thyroid-stimulating hormone(sTSH)of all patients were normal and TGAb was negative before IFNα therapy.After the treatment,10 patients(10/3 10,3.22%)had thyroid dysfunction,in which 7 were of hypothyroidism and 3 were of hyperthyroidism.The thyroid function of all patients recovered within 1-year follow-up.Conclusions IFNα therapy may induce thyroid dysfunction in patients with chronic hepatitis,in which prognosis can be good when appropriate treatment is given.

Objective To study the effects of melatonin on the expression of endothelin-1 (ET-1) and endothelial nitricoxide synthase (eNOS) in renal cortex of insulin-resistant (IR) rats. Methods Insulin-resistant rat model was established with 6-8 week ages Sprague-Dawwley (SD) rats by high-glucose diet (70% calories from glucose) for 6 weeks. IR rats models were divided into control normal group (CN, n=10), IR group (n=10) and melatonin group (MEL, n=10). Rats in MEL were affused melatonin 10mg/(kg·d) (4PM) for further 6 weeks. The protein and mRNA in rats' renal cortex of each group were assayed by immunohistochemistry and RT-PCR. Results Arterial blood pressure (ABP) and serum triglyceride (TG), free fatty acid (FFA), malondialdehyde (MDA), insulin (Ins) and homeostasis model analysis insulin resistance (HOMA-IR) in MEL were lower than that in IR (all P＜0.01). Serum high-density lipoprotein (HDL-c) and superoxide dismutese (SOD) were higher in MEL than that in IR (all P＜0.01). HE staining showed that the structure of renal cortex in MEL is approximately normal. The levels of ET-1 protein and mRNA in renal cortex of MEL rats were lower than that in IR group. The levels of eNOS protein and mRNA of renal cortex in MEL group were higher than that in IR group (all P＜0.05). Conclusion At the early stage of IR, melatonin application can ameliorate the imbalance between ET-1 and eNOS and protect the angio-endothelial function of renal cortex in high-glucose induced IR rats.

Objective To explore the functions of neuron‐specific enolase(NSE) and human multiple myeloma U266 cells on osteoclast‐like cells(OLC) function .Methods Normal human peripheral blood mononuclear cells were induced and cultured by adding RANKL and M‐CSF to get OLC ;the experiment was divided into 3 groups ,the NSE group:OLC were cultured in the 6‐well culture plate for 14 d and added with 100 ng/mL recombinant human NSE to culture for 24 ,48 ,72 h;the co‐culture group:OLC were cultured in the lower well of 6‐well Transwell chamber for 14 d ,then added with 1 × 105/well U266 cells in each upper well and conducted the co‐culture for 24 ,48 ,72 h;the control group :OLC were cultured alone .The influences of NSE and U266 cell line on RANKL ,OPG ,IL‐6 and TRAP mRNA transcriptional level of OLS were compared by using real‐time fluorescent quantitative PCR .Results RANKL ,OPG ,IL‐6 mRNA had no expression on OLC in the co‐culture group ,NSE group and control group ;com‐pared with control group ,the TRAP mRNA expression level in the co‐culture group and the NSE group was increased ,the differ‐ence was statistically significant(P<0 .01);the increase of TRAP mRNA expression level was obvious especially at 48 ,72 h .Con‐clusion OLC expressing TRAP and NSE may be one of the factors for promoting OLC differentiation and maturation in myeloma bone disease ,prompting that NSE could increase the OLC viability .

Aim To discover specific telomerase inhibitors by high-throughput screening in the more than 2 000 strains of actinomycetes fermentation broth.Methods After verifying the three kinds of screening models for telomerase inhibitor by yeast PCR,the three yeast strains were inoculated overnight into yeast liquid culture which was lacking in histidine and contained 3-AT.Then concentration of yeast strains in culture liquid and dosage of screening sample were regulated for better adjustment to the high-throughput screening.At last the adjusted liquid culture containing yeast strain piped to 96-well plates,drug samples and control article were also added,then the yeast was incubated in the thermostat oscillator.At the same time OD_(595nm) of the yeast liquid was measured every 12 h by using Multifunctional Microplate Analyzer,and the samples whose inhibitory efficiency was over 0.45 were selected for secondary screening.Results The high-throughput screening method related to yeast three-hybrid system was effective in screening samples that had potential telomerase inhibitory ability.The initial concentration of yeast liquid OD_(595nm) was 0.04 and the best dosage of samples was 5 ml.14 active samples,whose inhibitory efficiency was over 0.45,were founded after primary and secondary screening.Conclusions High-throughput screening method related to yeast three-hybrid system is simple and stable in discovering telomerase inhibitors,and 14 new antitumor compounds are identified,which lays the foundation in the development of new anti-tumor agents.