Objective:To explore the role of clinical pharmacist in clinical medication by the pharmaceutical care for a patient with acute myocardial infarction complicated with gastrointestinal bleeding .Methods: Clinical pharmacist made an individualized pharma-ceutical care and provided medication guidance for the patient according to the characteristics of acute myocardial infarction and gastro -intestinal bleeding combined with the patient ’ s self factors .Results:The pharmaceutical care improved the compliance of the patient and the efficacy and safety of the drug treatment .Conclusion:The safety and rationality of drug use can be improved by the practice of clinical pharmacist in clinical medication .

Objective To evaluate the characteristics of echocardiogram in senile degenerative heart valvular disease. Methods The cardiac structure and functional changes of the 166 cases of senile degenerative heart valvular diseases were studed with HP5500(USA) and En Visor color doppler(Philips). Results Simple aortic valve calcification was found in 93 cases(56.0%); simple mitral calcification, 18 cases(10.8%);aortic valve calcification combining mitral calcification, 55 cases,(33.1%);enlarged left atrium 116 cases(69.9%);left ventricular diastolic dysfunction 142 cases(85.5%).Within 136 cases of cardiac valve dysfunction, there was aortic valvular regurgitation in 70 cases(42.2%),stenosis of aortic valve in 23 cases(13.9%),mitral regurgitation in 20 cases(12.2%),mitral stenosis in 8 cases(4.8%),aortic valvular regurgitation combining stenosis in 10 cases(6.0%),mitral regurgitation combining stenosis in 5 cases(3.0%). The highest morbidity in valvular dysfunction was aortic valvular regurgitation(42.2%),the second was aortic valve stenosis(13.9%),the lowest morbidity was mitral stenosis combining insufficiency(3.0%). Enlarged left atrium was in 116 cases(69.9%). Conclusions The senile degenerative heart valvular disease have no specific clinical manifestation. With increasing age,the proportion of complex valve calcification is increased, and the highest is the aortic insufficiency in valvular dysfunction. The proportion of enlarged left atrium is also increased.

Objective: To explore the role of clinical pharmacist played in treating patients with acute myocardial infarction. Methods:The clinical pharmacists participated in the therapy for a patient with acute myocardial infarction and provided individual pharmaceutical care. The clinical pharmacist optimized the therapy by means of recognizing the drug-drug interactions and adverse drug reactions as well as evaluating the risk of thrombosis versus bleeding for the patient. Results:The therapeutic suggestions of the clinical pharmacist were accepted by the physicians. The patient was treated effectively and discharged from hospital. Conclusion: Clinical pharmacist can provide beneficial contributions to safe, effective and optimal medication.

Objective:Spectrophotometer is a device to be verified obligatory in hospital. This article introduced some techniques to avoid ordinary mistakes in measurement. Methods:We introduced principle and verification regulation of spectrophotometer. Such methods as parameter configuration, proper maintenance can reduce our mistakes. Results: This article analyzed some common problems in measurement and also offered solutions. Conclusion: In the measurements of spectrophotometers, because the operators have less spectrophotometer operation experience, they often get inaccurate data which lead to an error. Conclusion: To avoid these mistakes, strictly regulate operations in spectrophotometer’s verification and proper routine maintenance are necessary.

Objective To evaluate the analytical variables of the reversed-phase high pressure liquid chromatography (HPLC) with 5-Fluorouracil(5-Fu) as an internal standard for determining creatinine in human serum and urine. The method was used as a candidate reference method for accuracy assessment of routine creatinine test kits.Methods We tested the accuracy, precision, linearity of the reversed-phase HPLC.We analyzed split samples of a panel of 85 patients’ serum and 94 patients’ urine and compared the results of the routine test kits and HPLC by means of bias plots (percentage differences of results) and standard linear regression.Results The linear range of HPLC method was up to 2 210 ?mol/L, the within-run CV(n=5) was below 2.5% and the between-day CV(n=10) was less than 4.5%. The analytical recovery rate was 96.3%～102.4%. All of the test kits correlated very well with HPLC.Conclusions We recommend the reversed-phase HPLC with 5-Fu as an internal standard as a candidate reference method for determining creatinine in human serum and urine.The enzymatic method with creatininase coupled sarcosine oxidase is suitable for routine work in clinical laboratories.

OBJECTIVE To evaluate the medical instrument′s cleaning effect and bacterial elimination result by using multi-enzyme cleaning agent.METHODS The total 229 pieces of moderate contaminated medical instrument were randomly divided into 2 groups:control group and experiment group.The effects after routine water immersion and processing of multi-enzyme cleaning(including hand cleaning and machineone were observed and the bacteriological examination in the sample was checked.RESULTS 96% of the medical instruments in experiment group were cleaned,while only 35% of them in control group were cleaned.It was showed the cleaning effect of multi-enzyme was better than routine water immersion.In the experiment group,the contaminated rates of blood pincers and tweezers after hand cleaning with multi-enzyme were 33.7% and 25.5%,respectively,while the contaminated rates of blood pincers and tweezers after machine cleaning with multi-enzyme were 0.It indicated that the bacterial eliminate rate of machine cleaning with multi-enzyme was higher than hand cleaning with multi-enzyme.CONCLUSIONS Multi-enzyme agents are better for medical instrument cleaning.

AIM: To study the effect of chronic hypoxia(CH) on the intracellular calcium([Ca~(2+)]i) in pulmonary artery smooth muscle cells(PASMCs) and the role of L-type calcium channel and calcium store. METHODS: The rat chronic hypoxia model was set up and intervene the PASMCs with normal PSS,calcium-free PSS,nifedipine,and heparine respectively.The resting [Ca~(2+)]i was determined with the Fura-2/AM calcium imaging technique.RESULTS:(1) The [Ca~(2+)]i in CH group in normal PSS was higher than that in control group in normal PSS(P

With the in-depth research of blood circulation and blood stasis, many scholars gradually carried out research and exploration the pathophysiological basis of blood stasis of the ankylosing spondylitis (AS), from the blood rheology, microcirculation, platelet activation, vascular endothelial function and so on. Some progress that been made were summarized below.

Objective To investigate the direct effect ofAntiphlogistic No.1 on the degranultion of RBL-2H3 mast cells stimulated by anti-DNP IgE and DNP-BSA complex.Methods The effect of Antiphlogistic No.1 on stabilization of RBL-2H3 cell membrane was assessed by degranultion inhibition rate.β-hexosaminidase and IL-4 released from RBL-2H3 cells were detected by PNAG colorimetric assay and ELISA.Results β-hexosaminidase and cytokine IL-4 production releases from RBL-2H3 cells was reduced after they had been treated with Antiphlogistic No.1.The inhibition rate of β-hexosaminidase release was 42.47％ at the concentration of 8 μg/ml (P＜0.01) and dose dependently increased to 52.40％,68.26％ and 72.15％ respectively when drug concentration up to 80 μg/ml,800 μg/ml,8000 μg/ml,while inhibition rate of IL-4 generation were 13.87％,23.27％,31.95％,39.99％ (P＜0.01).Antiphlogistic No.1 maximum inhibition rate of β-hexosaminidase release is 84.48％ of dexamethason maximum inhibition rate while Antiphlogistic No.l maximum inhibition rate of IL-4 is close to dexamethason maximum inhibition rate.Conclusion Antiphlogistic No.1 could stabilize the cellular membrane of RBL-2H3 and provide protection against Ⅰ type allergic response.

Objective To evaluate tumor angiogenesis and clinical significance by contrast-enhanced CT in case of non-small cell lung cancer (NSCLC). Methods 30 patients with NSCLC underwent dynamic thoracic CT, and histopathological slides were carefully prepared for VEGF immunohistochemical staining. Maxium attenuations of dynamic CT were compared with VEGF expression levels and lymph-node metastases. Results The mean peak attentation was (36.28?6.41)HU, VEGF positive expressions were in 21 patients, and negative expressions were in 9 patients. VEGF expression levels in patients with NSCLC stage II and III were higher than those in stage I , and in lymph node metastases group, the expression levels of VEGF were also higher than those in non-metastatic nodes. CT enhancement of NSCLC was positively related to VEGF expression,neoplasm stage and lymph-node metastases. Conclusion CT enhancement of NSCLC can reflect tumor angiogenesis and correlate to lymph-node metastases closely, help lung cancer diagnoses,neoplasm stage, and serve as a supplement to the present staging system for lung cancer in biological behavior.