The growth and metastasis of tumor relies on oxygen and nutrients provided by neonatal blood vessel. When no angiogenesis, the tumor growth will be obviously inhibited. At present, anti-tumor angiogenesis strategies are increasingly becoming a focus of attention and study at home and abroad. However,because of the complexity of the antiangiogenesis, the clinical effects of antiangiogenesis alone is not so satisfied, while this is reversed when combined with radiotherapy.

[Objective] To optimize the exact preparative technique of complex prescription Dragon’s Blood Plaster(DBP),to evaluate the therapeutic effect to endometriosis rats with acupoint therapy and to discuss its probable mechanism.[Methods] Making into DBP through the preparation of ultra-fine powder,extraction of the volatile oils and their ointments were made as well.Induced by surgery for the experimental endometriosis ,observed that the levels of PGF2a in the blood plasma and IL-1? in the blood serum in different control groups.[Results] Complex prescription DBP could inhibit the ectopic endometrium hyperplasic ,and decrease the levels of PGF2a.No significant difference in IL-1? in the blood serum.[Conclusion]The treatment by acupoint therapy with complex prescription DBP is effective to endometriosis.It could inhibit the ectopic endometrium hyperplasic and promote the shrinking of glands.

Objective To investigate the risk factors and prognosis of pneumonia in elderly patients of cerebral stroke sequelae period.Methods Eighty-eight cases of pneumonia in elderly patients with cerebral stroke sequelae period were selected as stroke group,with non-stroke patients of the same period suffering from pneumonia 1 ∶ 1 matched as control group.The risk factors and prognosis of pneumonia in elderly patients of cerebral stroke sequelae period were analyzed.Results Pneumonia in stroke group was related with hypertension,diabetes,conscious disturbance,bucking or nasogastric feeding,dyscinesia,incision of trachea,multiple organ disfunction syndrome (MODS),hospitalizations,hospital stay and abandon treatment or death (P ＜ 0.05),and not related with smoking,coronary heart disease,kindey dysfunction,malignant tumor,phlegm bacterial culture,hypoxemia and mechanical ventilation was tested by conditioned Logistic regression quantitatively(P＞ 0.05).The independent risk factors for pneumonia in elderly patients of cerebral stroke sequelae period were bucking or nasogastric feeding,and MODS by two-way unconditional Logistic regression.Conclusions Hypertension,diabetes,conscious disturbance,bucking or nasogastric feeding,dyscinesia,incision of trachea,MODS,hospitalizations are risk factors for pneumonia in elderly patients of cerebral stroke sequelae period,and lead to relatively poor prognosis.Three-grade prevention and control of risk factors can improve prognosis.

RNA interference (RNAi) is a post-transcriptional suppression method of introduction of double-stranded RNA molecules by artificially induced. Recently RNAi has been widely used in xenografted tumors of nude mice in vivo. In order to inhibit the growth of tumor, some genes related with tumor such as anti -apoptosis gene, proliferation promoting gene, metastasis-associated gene are depressed via RNAi. Additionally, in the nude mice test, RNAi is utilized as assisting methed of radiotherapy and chemotherapy of human tumors.

Clinical data of 365 patients with type 2 diabetes admitted from December 2010 to May 2011 were retrospectively analyzed,including 130 cases with fatty liver and 235 non-fatty liver cases.There were no differences in gender,age,disease course between two groups (P ＞ 0.05). BMI,glycated hemoglobin (HbAlc),blood pressure,TC,TG,LDL-C,large vessels complications and microvascular complications rate in fatty liver group were significantly higher than those in non-fatty liver group ( all P ＜0.01 ),while there was no difference in rate of peripheral neuropathy (P ＞ 0.05 ).The results indicate that blood glucose,blood pressure,BMI,abnormal blood lipid and chronic complications were closely related to type 2 diabetics with fatty liver.

Objective To explore the value of breast-duct endoscopy-assisted epidural catheter-guided tumor localization in the resection of intraductal lesions.Methods A total of 45 cases of breast-duct endoscopy-confirmed intraductal tumor were enrolled in this study.After the depth and location of the tumor was determined,the endoscope was withdrawn and an epidural catheter was inserted into the duct with discharge at a same level to support the duct and guide the resection of the tumor.Then,the skin covering the nipple and areola were cut radially,and the diseased duct was resected under the guidance of a epidural catheter.Results The ductoscopy showed intraductal papilloma in 41 cases,multiple intraductal papillomas in 2,and papillomatosis in 2.In the 41 patients with papilloma,the diagnosis was confirmed pathologically after the operation in 39 cases(95%),while in the 2 patients with multiple papillomas and the 2 with papillomatosis,the identical rate was both 50%(1/2).The misdiagnosed cases included 2 patients with epithelial hyperplasia,1 with multiple intraductal papillomas complicated with atypical ductal hyperplasia and severe focal hyperplasia,and 1 patient with breast cancer.In all the cases,the shape of the breast and nipple were normal after the operation.Conclusion Breast-duct endoscopy-assisted epidural catheter-guided tumor localization is worth being widely used for resection of intraductal lesions in local hospitals,for its precise localization,simple procedure,and good cosmetic outcomes.

Using PBL Teaching Mode in the clinical diagnosis pathological teaching of Postgraduate during clinical practice is not only good for students to arouse the interests of learning,consolidate their pathological knowledge and enhance pathological diagnosis consciousness, but also helps improve their general capabilities of clinical pathological diagnosis thinking,analyzing and solving problems. It does lay a solid foundation for the postgraduates to become clinical pathologists.

Based on competence,this article studied on curriculum reform of nursing pharmacology.It described the model of curriculum construction on making teaching target,training professional quality,designing optimum learning situation,reforming teaching method and constructing curriculum evaluation system.This model is more suitable for nursing work and provide guarantee for cultivating high skilled and applicable nursing talent.

Objective To investigate the effect of renal anemia variability on the progression of renal function in patients with chronic kidney disease and hemoglobin variability on the incidence of cardiovascularevents,mortality and re-hospitalization rate.Methods one hundred and twenty-three patients with chronickidney disease with stage Ⅲ-Ⅴ were enrolled in this study.The information of hemoglobin variability,creatinineclearance rate (Ccr),renal function,the incidence of cardiovascular events,mortality and rehospitalization rate,the incidence of cardiovascular events,mortality and rehospitalization rate were recorded and analyzed.ResultsThe change of hemoglobin level was (20.6 ± 10.7) g/L and Ccr change level was (16.3 ± 4.1) ml/min inpatients,and the pearson correlation coefficient was 0.82.Multiple covariance analysis showed that Ccr increaselevel was higher in CKD Ⅲ-Ⅴ period patients with hemoglobin fluctuations than that of hemoglobin stabilitypatients after adjusting smoking history,blood pressure,24 h urinary protein quantitative factors (F =7.6,9.1,4.3;P＜0.05).The incidence of cardiovascular events in patients,mortality,rehospitalization rate of hemoglobinwave group were higher than those of hemoglobin stabilizing group(x2 =26.9,5.1,10.6,P＜0.05 or P＜0.01).Conclusion The fluctuation of hemoglobin is able to aggravate quickly of renal function development,increasethe incidence,mortality and rehospitalization rate of cardiovascular events.

Objective To research the effect of type 2 diabetes on collagen content in different organsinOLETFrats.Methods 24maleOLETFratsweregivenhigh‐caloriedietfor30weekstoset up the T2DM. At the 30th week ,16 male T2DM OLETF rats were selected as case group ,and 12 LETO rats were used as control group. The skin ,liver ,and femur tissue were collected at the same time. HE and Masson staining and immunohistochemical test were performed to measure the content of collagen and HPLC was used to detect hydroxyproline. Results Compared with the NC group ,DM group showed the collagen fibers were decreased in femoral and skin tissue ,but increased in liver tissue. The collagen content of the skin tissue was significantly lower in DM group than in NC group [(149.9 ± 18.4) vs (266.0 ± 54.8)mg/g ,P<0.01] ,and the collagen content of the femur tissue was also lower in DM group than in NC group [(185.60 ± 19.38) vs (243.27 ± 22.19)mg/g ,P<0.05]. In contrary ,the collagen content of liver tissue was higher in DM group than in NC group [(134.54 ± 18.78) vs (64.52 ± 21.51) mg/g ,P<0.01]. Conclusion The effect of type 2 diabetes on the collagen in different organs is different. The collagen content decreases in the femoral and skin tissue but increases in the liver.