Aging changes in acetylcholinesterase positive (AChE-P) neurons of the globus pallidus were investigated histochemically and morphometrically in young (3 months old) and old (24 months old) Spragur-Dawley male rats. The number of the positive neurons in the old group is decreased by 11.8％ in comparison with the young group. The total process length of the AChE-P neurons in the young rat is approximately 1.4 times as that in the old rat. The length of about 8.6％ of the positive neurons in the old group, however, exceeds the average length of AChE-P cellls in the young group (232.1 ?m). In the old rat, the gray value of AChE-P neurons of the globus pallidus is notably higher than that in the young rat, but the value of nearly 6.8％ of the positive cells in 24-month-old rat is inferior to the average value in 3-month-old rat (117.8). The transverse dimensions of AChE-P cell bodies in the old group are increased by 9.2％ as compared to those in the young group. Morphological observations show that most of AChE-P neurons in the old rat globus pallidus represent a typical degenerative alterations, while a substantial number of the positive neurons in the old animal are characterized by enlarged bodies, strong histochemical reaction as well as dense processes and their branches. The above findings indicate that, in the old rat, a decline of AChE histochemical reactivity and the morphological degeneraton of AChE-P neurons with the advanced age do not occur synchronously in all the AChE-P neurons of the globus pallidus. Therefore, it is suggested that there probably exist a compensative mechanism in senescence of the globus pallidus.

Objective To investigate the activation of nuclear factor-κB(NF-κB),which was stimulated by angiotensin-Ⅱ(AngⅡ)through classical pathway in human pulmonary microvascular endothehal cells(HPMEC).Method The experiment was divided into two groups:in Ang Ⅱ group,HPMECS were incubated with 10-6mol/L AngⅡ for 0,0.5,1,2 and 4 hours,respectively;in losartan group,HPMEC was pretreated with 10-6mol/L losartan(inhibitor of AngⅡ type 1 receptor)for 1 hour,and then stimulated with 10-6mol/L AngⅡ for 2 hours,and the nucleax protein and the cell plasma protein were prepared by lysis and centrifugation.Electrophoretic mobility shift assay(EMSA)was used to detect the NF-κB DNA binding activity.The inhibitor of κBa(IκBα)was detected by Western blotting.The data were expressed as(x±s)and analyzed with one way analysis of variance.A P value less than 0.05 indicated significant difference.Results Compared with the activity of NF-κB at 0 h (100.0±25.1)after AngⅡstimalation,the activity increased significantly at 0.5 hour(144.5±16.1,P＜0.05),and reached peak value at 2 hours(270.1±27.2,P＜0.05).The concentration of IκBα at 0 hours was 44.4%±2.1%,decreased markedly at 0.5 hours(38.9%±3.6%,P＜0.05),and to the lowest level at 2hours(32.6%±2.3%,P＜0.05).The activity of NF-κB(115.4±10.7)and the concentration of IκBα(43.6%±3.7%)in losartan group had ilo significant difference with AngⅡ group at 0 h(P＞0.05).The activity of NF-κB and the concentration of IκBα in losartan group had significant difference with AngⅡ group at 2hours.Conclusions NF-κB can be activated through classical pathway,which stimulated by AngⅡ in HPMEC.

Ninety elderly patients with coronary heart disease were randomly divided into 2 groups:in study group (n = 45) the cognitive-behavioral intervention as well as conventional medical treatment were performed and in control group ( n = 45 ) only conventional treatment was given. Short Form-36 ( SF-36),symptom checklist 90(SCL-90), Hamilton anxiety scale( HAMA), Hamilton depression scale( HAMD)were evaluated before and after intervention, the serum total cholesterol (TC), triglyceride (TG)and body mass index (BMI)were measured, the smoking and drinking behaviors were recorded. After 6-month of intervention the smoking [(0. 6 ± 0. 2)cigarettes/d] and drinking [(10. 1 ± 2. 3)g/d] behaviors, the scores of SF-36 (85 ± 16), SCL-90 (104 ±4), HAMA (10 ±4) and HAMD (12 ±3), the systolic blood pressure [( 123 ± 11 ) mm Hg ( 1 mm Hg = 0. 133 kPa)], diastolic blood pressure [(77 ± 5 ) mm Hg], the fasting blood glucose [(4. 2 ± 2. 6 ) mmol/L] and BMI ( 22.5 ± 0. 5 ) in study group were significantly improved compared to control group ( P ＜ 0. 01 ). The results indicate that cognitive-behavioral intervention can improve the mental state and quality of life in elderly patients with coronary heart disease.

Objective To evaluate the effectiveness of chronic diseases self-management model for the aged patients with type 2 diabetes mellitus in communities.Methods Patients with T2DM aged over 60 years and over in two communities in Baoding were selected:one as intervention community and the other as control community.The intervention community underwent the chronic diseases self management theory based education by expert,face to face consultation and patient communication,while the control community received general community management.The scores of self-efficacy were collected before the sessions and 3 months after the end of the sessions,and the effectiveness of the intervention was evaluated by the changes of scores,fasting blood-glucose and hemoglobin A1c (HbA1c) before and after the session.The pure increment level of scores of diabetes self-efficacy was higher in the intervention community than in the control community [(16.9 ± 5.3)scores vs.(0.4±1.3) scores,t=21.317,P＜0.01].Results All dimension values were also significantly higher in the intervention group than in control community (P＜ 0.01).The pure decrement level of fasting blood glucose (1.8 ± 1.7) ％ and HbA1c (0.7 ± 1.2) ％ after intervention was significantly higher in the intervention than in control community (t=6.096,P＜0.01 ;t=2.930,P＜0.05).Conclusions The chronic diseases self-management education may significantly improve patients' self-efficacy and decrease the level of blood-glucose and HbAlc.

To investigate the immunological activities of the recombinant human phosphatase D2 (rhPLD2) in vitro and in vivo, especially its ability to reduce inflammatory reactions, the cDNA fragment encoding rhPLD2 was cloned into prokaryotic expression vector pET30a by RT-PCR and the recombinant protein rhPLD2 expressed in E.coli was purified from the inclusion bodies, while the anti inflammatory activity of rhPLD2 was determined by the amount of eosinophils in bronchoalveolar fluid(BALF) and blood and the expression of IL-5 and MMP-9 in lung tissues of guinea pig model of chronic asthma. It was found that the rhPLD2 recombinant protein was obtained from human Daudi cells by cloning to E.coli, which contained no membrane-binding site and signal peptide. The cDNA sequence encoded 631 amino acid residues (GenBank Accession Number: AY178289). The purity of the rhPLD2 approached up to 76% with a bioactivity of 50.9745 units/L (0.9212 g/L). In addition, the anti inflammatory effect of rhPLD2 protein could be demonstrated in the guinea pig model of chronic asthma after treatment with rhPLD2 protein, such as down regulation in the expression of the inflammatory cytokine IL-5. It is concluded that the anti-inflammator activity of the recombinant human truncated PLD2 protein produced from the E.coli plasmid can be demonstrated both in vitro and in vivo.

Objective To investigate a new method of a long term culture for rat islets in vitro. Methods Rat islets marked by green fluorescent protein (GFP) were cocultured with Sertoli cell for 20 weeks. Histological studies were performed on islet group and coculture group in 1w, 3w, 10w, 15w, 20w of culture by light, fluorescent and electron microscopy. Insulin released was measured by radioimmunoassay. Results In islet group, islet viability and the number of insulin positive cells were significantly decreased after 3w of culture, cumulative quantities of insulin for 24 hours and the stimulation index also fell rapidly under this condition, meanwhile the ultrastructure of islets was destroyed. However, under coculture condition, culture time of islets was prolonged in vitro, islet viability and the number of insulin positive cells were significantly increased, cumulative quantities of insulin for 24 hours and the stimulation index maintained at high level, and the ultrastructure of islets remained normal even after 20w of culture. Conclusion Coculture of rat islets with Sertoli cells may promote islet growth and prolong culture time, and it is a new method of a long term culture of islets in vitro.

OBJECTIVE:To study the current situation and developing trend of the antidiabetics used in Guangdong re?gion.METHODS:The antidiabetics used in44hospitals in Guangdong region during the period2000～2003were analysed concerning the kinds of drug,sum of money for consumption and frequency of drug use.RESULTS:The sum of money for consumption of antidiabetics increased year by year so did its proportion in sum of money for consumption of total drug.Among them,consumption of insulin increased very quickly and oral antidiabetics assumed a tendency of increase as well.CONCLUSI_ ON:The variety of insulin is stable and new oral antidiabetics is going into market occasionally in this region,but frequency of use is primary to sulfonylurea,biguanides and glycosidase-inhibitors.

Objective To systematically review the efficacy of anodal transcranial direct current stimulation (A-tDCS) for relieving aphasia among stroke survivors.Methods The Chinese BioMedical Literature database,China National Knowledge Infrastructure,WanFang database,Chinese Scientific Journals full-text database,PubMed,Embase,MEDLINE and the Cochrane Library for clinical randomized and controlled trials were searched for reports about the use of A-tDCS in the treatment of aphasia after stroke.The resulting reports were retrieved manually and evaluated by two independent reviewers according to pre-set inclusion and exclusion criteria.The reports covered the period to November 2016.A meta-analysis was then conducted using RevMan software version 5.3.Results Seven studies covering 101 patients were selected for analysis.Meta-analysis showed that the average aphasia function score improved after A-tDCS significantly more than in the control group.Picture naming ability in stroke survivors with aphasia showed particular improvement.Only 11 patients in the 7 studies presented light adverse effects from AtDCS,always within the patients' tolerance range.Conclusion A-tDCS can significantly improve the speech of patients with aphasia after cerebral infarction.It is safe and easy to administer and is a rehabilitation technology worth popularizing and applying.Considering the limited number of articles,the conclusion remains to be further verified with randomized clinical trials of higher quality and a larger sample.

Objective: To investigate the association between frailty and type 2 diabetes mellitus (T2DM) using a bidirectional two-sample Mendelian randomization (MR) analysis, so as to provide the evidence for the prevention and control of frailty and T2DM. Methods: Frailty status was assessed using the Fried Frailty Score (FFS), with aggregated data sourced from 386 565 European samples in the UK Biobank. The T2DM data were obtained from the summary results of genome-wide analysis published by the Diabetes Genetics Replication and Meta-analysis Consortium, excluding the UK Biobank data, including 455 313 European samples. Eligible single nucleotide polymorphism site were selected from the FFS and T2DM summary data as instrumental variables. The bidirectional MR analysis was performed using the inverse-variance weighted method. The heterogeneity was assessed using the modified Cochran Q test. The horizontal pleiotropy of instrumental variables was examined using MR-Egger regression. The robustness of the results was evaluated using the leave-one-out method. The bias was tested using funnel plot. Results: The forward MR analysis revealed a statistically significant association between increased FFS and an increased risk of T2DM (OR=2.280, 95%CI: 1.169-4.447). The reverse MR analysis showed a statistically significant association between T2DM and increased FFS (β=0.026, 95%CI: 0.012-0.041). Both bidirectional MR results were robust, with no horizontal pleiotropy of the instrumental variables was found (all P>0.5); and the funnel plots did not show significant basis. Conclusion The study found a bidirectional causal association between frailty and T2DM.

OBJECTIVE: To investigate the association of MTHFR C677T and MS A2756G polymorphism with semen quality in China. METHODS: The experimental group included 75 males with oligospermia, asthenospermia or teratospermia. The control group included 72 fertile males with normal fertility and sperm quality. The differences in the frequency of genetic polymorphism of MTHFR C677T and MS A2756G in the 2 groups were analyzed, and the plasma homocysteine (Hcy) level in both groups was detected. RESULTS: The frequency of MTHFR C677T genotypes (CT, TT and CT+TT) in the abnormal sperm group was higher than that in the control group (P<0.05), and it was the same case for T allele between the 2 groups (P<0.05). There was no difference in the frequency of MS A2756G genotypes between the two groups (P>0.05). The Hcy level in abnormal sperm group was higher than that in the control group. In all subjects, the Hcy level of the MTHFR genotypes (CT, TT and CT+TT) was higher than that of the CC genotype, with no difference among the three MS A2756G genotypes. CONCLUSION CT and TT genotypes of MTHFR C677T are associated with abnormal sperm, which might be part of the pathogenesis of abnormal sperms. T allele may be the risk factor in China. The one mechanism of the association between MTHFR C677T polymorphism and semen quality could be higher Hcy level. MS A2756G polymorphism may not associate with semen quality in China.
Alleles ; China ; Genotype ; Humans ; Male ; Methylenetetrahydrofolate Reductase (NADPH2) ; genetics ; Polymorphism, Genetic ; Risk Factors ; Semen Analysis