Objective To investigate the expression of epidermal growth factor-like domain 7 (EGFL-7) in brain tis?sue of diabetes mellitus (DM) rats, and the intervene effect of amioguanidine and baicalein thereof. Methods Forty-eight SD rats were randomly divided into following four groups:control group, diabetic group, aminoguanidine group and baicalein group (n=12 for each group). The control group received only pelleted food and water. Diabetic group, aminoguanidine group and baicalein group were given high fat and sugar diet in combination with small dose of intraperitoneal injection of strepto?zotocin to establish the diabetic model. And then aminoguanidine group and baicalein group were given aminoguanidine and baicalein 150 mg/(kg · d) through oral gavaging. Diabetic group and control group were given equivalent saline through oral gavaging. After 16-week treatment, the expression of EGFL-7 proteins in rat brain was detected by immunohistochemistry and Western blotting methods. Results Results of immunohistochemistry and Western blotting assay showed that the ex?pression of EGFL-7 was significantly increased in diabetic group, aminoguanidine group and baicalein group compared with that of control group (P<0.01). When compared with diabetic group, the expression of EGFL-7 was significantly decreased in aminoguanidine group and baicalein group (P<0.01). There was no significant difference in the EGFL-7 expression be?tween aminoguanidine group and baicalein group (P>0.05). Conclusion EGFL-7 may play an important role in cerebral vascular hyperplasia and remodeling of diabetic rats. Aminoguanidine and baicalein restrained cerebral vessel growth by re?ducing the expression of EGFL-7.

Objective To analyze the quality of life(QOL) of gastric cancer patients who underwent different types of gastrectomy and digestive tract reconstruction procedures.Methods Among the patients who(underwent) gastrectomy in our department over a period of 4 years,61 cases that survived for more than 2 years were analyzed for QOL using 14 parameters.The 61 cases included 13 cases of proximal subtotal(gastrectomy) and esophagogastrostomy,18 cases of total gastrectomy and jejunal P pouch reconstruction,and 30 cases of total gastrectomy and double Braun type of jejunal reconstruction.Pouch emptying function in 12 patients with different types of reconstruction was determined in by Isotope ~(99m)TC scintigraphy.Results At 6 months after operation,the parameters showed no significant differences between the 3 groups.At 12 months after operation,the QOL of patients with double Braun-type reconstruction was superior to that of patients with P jejunal pouch and patients with esophagogastrostomy in intake time(P

Objective To evaluate the using and results of computer-aided detection for full-field digital mammography in Chinese women. Methods 934 individuals more than 40 years old from a specific unit in Shenyang city were screened by full-field digital mammography ( Senographe 2000D, GE) . The examination images were diagnosed firstly by the experienced radiologists and then were analyzed by the computer-aided detection system ( R2' Image Checker ) , respectively. The results from these two assays were compared. Results The prompts were generated in 799 out of 1734 normal images, false-positive rate of computer-aided detection ( CAD) was 46. 1 % in the cases with normal breast image. The correct prompts rate was 70. 5 % in the cases with breast mass, 75. 0 % in the cases with breast microcalcification and 100. 0 % in the cases with breast cancer. Conclusion Although the CAD system has showed clinical values in practice, there are still some false-positive and false-negative results.

The factors influencing the long-term survival of patients with proximal gastric cancer (PGC) after curative resection were investigated. Data from 171 patients who underwent curative resection for PGC were retrospectively analyzed. The patients were grouped according to the clinicopathological factors and operative procedures. The tumor depth (T stage) and lymph node metastasis (pN stage) were graded according to the fifth edition of TNM Staging System published by UICC in 1997. The metastatic lymph node ratio (MLR) was divided into four levels: 0%, <10%, 10%-30% and >30%. The data of survival rate were analyzed by Kaplan-Meier method (log-rank test) and Cox regression model. The 5-year overall survival rate of 171 patients was 37.32%. The univariate analysis demonstrated that the survival time of the postoperative patients with PGC was related to tumor size (chi (2)=4.57, P=0.0325), gross type (chi (2)=21.38, P<0.001), T stage (chi (2)=27.91, P<0.001), pN stage (chi (2)=44.72, P<0.001), MLR (chi (2)=61.12, P<0.001), TNM stage (chi (2)=44.91, P<0.001), and range of gastrectomy (chi (2)=4.36, P=0.0368). Multivariate analysis showed that MLR (chi (2)=10.972, P=0.001), pN stage (chi (2)=6.640, P=0.010), TNM stage (chi (2)=7.081, P=0.007), T stage (chi (2)=7.687, P=0.006) and gross type (chi (2)=6.252, P=0.012) were the independent prognostic factors. In addition, the prognosis of patients who underwent total gastrectomy (TG) was superior to that of patients who underwent proximal gastrectomy (PG) for the cases of tumor >/=5 cm (chi (2)=6.31, P=0.0120), Borrmann III/IV (chi (2)=7.96, P=0.0050), T4 (chi (2)=4.57, P=0.0325), pN2 (chi (2)=5.52, P=0.0188), MLR 10%-30% (chi (2)=4.46, P=0.0347), MLR >30% (chi (2)=13.34, P=0.0003), TNM III (chi (2)=14.05, P=0.0002) or TNM IV stage (chi (2)=4.37, P=0.0366); and combining splenectomy was beneficial to the cases of T3 (chi (2)=5.68, P=0.0171) or MLR >30% (chi (2)=6.11, P=0.0134). It was concluded that MLR, pN stage, TNM stage, T stage, and gross type had advantages in providing a precise prognostic evaluation for patients undergoing curative resection for PGC, in which MLR was the most valuable index. TG and combining splenectomy were useful to improve the prognosis to patients with PGC of TNM III/IV stage, serosa invasion, or extensive regional lymph node metastasis.

0.05). Expression of MDR1 had a positive ~correlation with mutant p53 accumulation and HER2 expression(P0.05 ).In univariate analyses,TNM staging, axillary lymph node metastasis, mutant p53 accumulation, and HER2 over-expression were negatively correlated with DFS and OS, and MDR1 over-expression significantly reduced OS but not DFS. In multivariate analysis, axillary lymph node metastasis, over-expression of MDR1 and HER2 were independent risk factors for prognosis. Conclusions ~Induction of multidrug resistance and poor response to chemotherapy and endocrinotherapy may be the chief reasons for poor prognosis of breast cancer with mutant p53 accumulation, and HER2 and MDR1 over-expression. ~Determination of the above genes′expression in breast cancer tissue can be of use in deciding the degree of ~malignancy , metastasis phenotype and prognosis of brest cancer. Increasing anthracycline dose may increase the ~overall response rate to chemotherapy and improve prognosis in patients with mutant p53 accumulation, HER2 and MDR1 over-expression, especially HER2 over-expression.

Objective To evaluate the correlations of serum level of thyroid-stimulating hormone (TSH) with levels of inflammatory factors lipoprotein associated phospholipase A2 (Lp-PLA2), tumor necrosis factor-α(TNF-α) and interleukine (IL)-6 in ischemic stroke (IS) patients with subclinical hypothyroidism (SCH) and its clinical significance.MethodsTwenty SCH patients, 30 IS patients and 30 IS+SCH patients, admitted to our hospital from October 2017 to October 2018, were enrolled in our study; 27 subjects underwent physical examination in our hospital at the same period were selected as the control group. The serum levels of TSH, Lp-PLA2, TNF-αand IL-6 were measured by up-converting phoshor assay and enzyme - linked immunosorbent assay with double antibody sandwich. The common carotid artery, internal carotid artery and vertebral artery were examined by Doppler ultrasonography to analyze the morphology, location, and echo characteristics of the plaques and stenosis rates. Pearson correlation analysis was used to analyze the correlations of TSH with inflammatory factors, carotid artery stenosis rate.ResultsThe levels of Lp-PLA2, TNF-αand IL-6 in IS+SCH group were significantly higher than those in SCH group, IS group and control group (P<0.05); those in the SCH group and IS group were significantly higher than those in the control group (P<0.05). The serum TSH levels in IS+SCH group and SCH group were positively correlated with Lp-PLA2 level (r=0.739,P=0.000;r= 0.697,P=0.000), TNF-αlevel (r=0.981,P=0.000;r=0.723,P=0.000) and IL-6 level (r=0.859,P=0.000;r=0.583,P=0.000); but the serum TSH levels in IS group and NC group were not correlated with Lp-PLA2 level (r=0.223,P=0.236;r=0.249,P=0.210), TNF-αlevel (r=0.304,P=0.103;r=0.056,P=0.780) and IL-6 level (r=0.027,P=0.887;r=0.138,P=0.491). The number of unstable plaques and degree of stenosis in IS+SCH group were larger/higher than those in IS group, SCH group and control group (P< 0.05); there was a positive correlation between serum TSH level and artery stenosis rate (r=0.739,P= 0.000).ConclusionSCH may lead to occurrence and development of atherosclerosis by increasing the serum levels of TNF-α, Lp-PLA2 and IL-6 caused by low-level chronic inflammation, which in turn increases the risk of ischemic stroke.

The development of behavioral medicine is a new and efficient way to improve the level of national health and the behavior intervention is the core content.There are three methods including story-tellingmethod,behavior-image method and patient-centered assessment and counseling for exercise and nutri-tion intervention(PACE)which have been proven to be effective.The psychological theory of homophily,self-regulation and problem solving provide a scientific explanation for the effectiveness of these three kinds of be-havior intervention.Ripple effect amplifies the effect of behavioral intervention and its psychological mecha-nism is self-determinism and self-regulation theory.In the future,the research of behavior intervention and its psychological mechanism needs to make new breakthroughs in aspects of theory,method and technology.

OBJECTIVETo elucidate the role of transforming growth factor-beta1(TGF-β1) in epithelial-mesenchymal transition of mesothelial cells and peritoneal metastasis of gastric cancer.

METHODSHMrSV5 cells, a human peritoneal mesothelial cell line, were incubated with TGF-β1, and their morphological changes were observed by phase contrast microscopy. Expressions of α-smooth muscle actin (α-SMA), vimentin, cytokeratin, E-cadherin, phosphorylated-Smad2 and Smad2 were examined by Western blotting. After fibroblastic-like mesothelial cells were co-incubate with HSC-39 cells(gastric cancer cell line), the adhesion and invasion potential of HSC-39 were evaluated by adhesion and invasion assay in vitro.

RESULTSFew mesothelial cells converted to spindle fibroblast-like morphology for 24 h, and remarkable phenotypic changes were observed at 72 h of TGF-β1 activation. TGF-β1 could induce α-SMA and vimentin expression, and down-regulate cytokeratin and E-cadherin expression in mesothelial cells (P<0.05). TGF-β1 induced phosphorylation of Smad2 within 15 min of stimulation, reached a maximum at 30 min after treatment and remained high level during the experiment without affecting total Smad2 expression(P>0.05). The percentage of HSC-39 gastric cancer cells adhered were significantly increased as compared to the control. When the mesothelial cells were treated by TGF-β1 for 72 h, the increased adhesion percentage was(146±17)%(P<0.05). After fibroblastic-like mesothelial cells co-incubated with HSC-39 cells for 48 h, more cancer cells [(61.1±11.4) cells/view field] invaded the coated membrane as compared to the control group [(31.9±8.1) cells/view field] (P<0.05).

CONCLUSIONTGF-β1 can induce the transition of mesothelial cells into myofibroblasts and Smad2 signal pathway may play a role in this transition, which is associated with increased adhesion and invasiveness of gastric cancer cells, and provides favorable environment for the dissemination of gastric cancer.

Cadherins ; Cell Line, Tumor ; Epithelial Cells ; Epithelial-Mesenchymal Transition ; Epithelium ; Fibroblasts ; Humans ; Peritoneal Neoplasms ; Signal Transduction ; Smad2 Protein ; Stomach Neoplasms ; Transforming Growth Factor beta1 ; Vimentin

The factors influencing the long-term survival of patients with proximal gastric cancer(PGC)after curative resection were investigated.Data from 171 patients who underwent curative resection for PGC were retrospectively analyzed.The patients were grouped according to the clinicopathological factors and operative procedures.The tumor depth(T stage)and lymph node metastasis(pN stage)were graded according to the fifth edition of TNM Staging System published by UICC in1997.The metastatic lymph node ratio(MLR)was divided into four levels: 0%,<10%,10%-30%and >30%.The data of survival rate were analyzed by Kaplan-Meier method(log-rank test)and Cox regression model.The 5-year overall survival rate of 171 patients was 37.32%.The univariate analysis demonstrated that the survival time of the postoperative patients with PGC was related to tumor size(;(2=4.57,P=-0.0325),gross type(χ2=21.38,P<0.001),T stage(χ2=27.91,P<0.001),pN stage(χ2=44.72,P<0.001),MLR(χ2=61.12,P<0.001),TNM stage(χ2=44.91,P<0.001),and range of gastrectomy (χ2=4.36,P=0.0368).Multivariate analysis showed that MLR(χ2=10.972,P=0.001),pN stage(χ2=6.640,P=0.010),TNM stage(χ2=7.081,P=0.007),T stage(χ2=7.687,P=0.006)and gross type(χ2=6.252,P=0.012)were the independent prognostic factors.In addition,the prognosis of patients who underwent total gastrectomy(TG)was superior to that of patients who underwent proximal gastrectomy(PG)for the cases of tumor ≥5 cm(χ2=6.31,P=0.0120),Borrmann Ⅲ/Ⅳ(χ2=7.96,P=0.0050),T4(χ2=4.57,P=0.0325),pN2(χ2=5.52,P=0.0188),MLR 10%-30%(χ2=4.46,P=0.0347),MLR >30%(χ2=13.34,P=0.0003),TNM Ⅲ(χ2=14.05,P=0.0002)or TNM Ⅳ stage(χ2=4.37,P=0.0366);and combining splenectomy was beneficial to the cases of T3(χ2=5.68,P=0.0171)or MLR >30%(χ2=6.11,P=0.0134).It was concluded that MLR,pN stage,TNM stage,T stage,and gross type had advantages in providing a precise prognostic evaluation for patients undergoing curative resection for PGC,in which MLR was the most valuable index.TG and combining splenectomy were useful to improve the prognosis to patients with PGC of TNM Ⅲ/Ⅳ stage,serosa invasion,or extensive regional lymph node metastasis.

Objective To elucidate the role of transforming growth factor-beta1 (TGF-β1) in epithelial-mesenchymal transition of mesothelial cells and peritoneal metastasis of gastric cancer. Methods HMrSV5 cells, a human peritoneal mesothelial cell line, were incubated with TGF-β1, and their morphological changes were observed by phase contrast microscopy. Expressions of α-smooth muscle actin (α-SMA), vimentin, cytokeratin, E-cadherin, phosphorylated-Smad2 and Smad2 were examined by Western blotting. After fibroblastic-like mesothelial cells were co-incubate with HSC-39 cells (gastric cancer cell line), the adhesion and invasion potential of HSC-39 were evaluated by adhesion and invasion assay in vitro. Results Few mesothelial cells converted to spindle fibroblast-like morphology for 24 h, and remarkable phenotypic changes were observed at 72 h of TGF-β1 activation. TGF-β1 could induce α-SMA and vimentin expression, and down-regulate cytokeratin and E-cadherin expression in mesothelial cells (P<0.05). TGF-β1 induced phosphorylation of Smad2 within 15 min of stimulation, reached a maximum at 30 min after treatment and remained high level during the experiment without affecting total Smad2 expression (P>0.05). The percentage of HSC-39 gastric cancer cells adhered were significantly increased as compared to the control. When the mesothelial cells were treated by TGF-β1 for 72 h, the increased adhesion percentage was (146 ±17)%(P<0.05). After fibroblastic-like mesothelial cells co-incubated with HSC-39 cells for 48 h , more cancer cells [(61.1 ±11.4) cells/view field] invaded the coated membrane as compared to the control group [(31.9±8.1) cells/view field] (P<0.05). Conclusion TGF-β1 can induce the transition of mesothelial cells into myofibroblasts and Smad2 signal pathway may play a role in this transition , which is associated with increased adhesion and invasiveness of gastric cancer cells , and provides favorable environment for the dissemination of gastric cancer.