Objective: To research the anti-inflammatory and anti-allergic effects of Qifangbimin particle.Methods: The anti-inflammatory effects were observed by the methods of xylene-induced ear-swelling in mice and cotton-ball induced granuloma in rats.The anti-allergic effects were evaluated by the method of passive skin allergy model in rats and ear-heterogeneous passive skin allergy model in mice.Results: In the treatment groups with Qifangbimin particle, the swelling degree of ear edema induced by dimethylbenzene in mice was significantly suppressed when compared with that in the control groups (P<0.01 or 0.05) , however, the particle had no significantly inhibitory effect on granulation tissue hyperplasia induced by cotton-ball in rats.The Qifangbimin particle groups obviously decreased the absorbance value of locus coeruleus on rats' back (P<0.01 or 0.05), and Qifangbimin particle at high dose significantly reduced the absorbance value of locus coeruleus of auricle in mice (P<0.01 or 0.05).Conclusion: Qifangbimin particle has significant anti-inflammatory and anti-allergic effects.

Objective To establish a simple animal model of cough variant asthma(CVA)through sensitizing Brown-Norway(BN)rats with ovalbumin(OVA). Methods A total of 36 BN rats were randomly divided into three groups, including the normal control group,the model control group and the montelukast group. BN rats in the model group and the montelukast group were intraperitoneally administered with 2.0 mg of OVA and 100 mg of Al(OH)3,and the same volume of sterile saline was given to the normal group by intraperitoneal injection. Boosting was carried out by intraperitoneal administration with 0.01 mg of OVA and 100 mg of Al(OH)33 weeks later,and the rats in the normal group were injected with the same dose of physiological saline. Three weeks later,the actively sensitized BN rats were challenged with aerosolized OVA for 7 times on alternative days,and the rats in the normal group were treated with sterile saline instead of OVA. At the same time, the montelukast group was given 1.3 mg/kg of montelukast 30 minutes before atomization by intragastric administration once a day for 2 weeks,and the normal group and the model group were given the same volume of water. The tests of cough sensitivity to capsaicin and bronchial responsiveness were performed 24 h after the last administration. Results Compared with the normal group, the times of coughing(P< 0.01)and the lung resistance(RL)(P< 0.05)in the model group were significantly increased,while the lung compliance(Cdyn)was significantly decreased(P< 0.05). There was a significant difference(P < 0.05)in the times of coughing caused by capsaicin between the model group and the montelukast group. Compared with the model group,RLin the montelukast group was decreased significantly(P< 0.05), and Cdynwas increased significantly(P< 0.05). Conclusions This rat model of CVA is similar to a variety of clinical features of CVA and is easy to operate. Thus it can be used as an effective animal model of CVA.

Objective To study the effects of Chinese medicine compound of warming and moistening lung with tonifying spleen(hereinafter“TCM”)on behavior,serum IgE and IL-6,as well as nasal muco-sa pathological changes of guinea pigs with allergic rhinitis.Methods SPF grade guinea pigs,half male and half female,were randomly divided into normal group,model group,Biyankang group (0.41 g/kg),loratadine group (0.91 mg/kg),TCM high-dose group (3.04 g/kg),mid-dose group(1.52 g/kg)and low-dose group(0.76 g/kg).Except the normal group,other groups were followed by bilateral-nostrils intranasal inhalation once daily with 10% TDI olive oil solution(5 μL each nostril),7 days in a row.After the model was established successfully,the guinea pigs were i.g.corresponding medication, with dripping TDI continuously for 14 days every other day.Nasal symptoms of all groups were observed and recorded.The serum contents of IgE and IL-6 were determined by ELISA,nasal mucosa pathological morphological changes were observed under light microscope,and number of eosinophils (EOS)were counted.Results Compared with the model group,scores of behavioral symptom were significantly low-er in both TCMhigh-and mid-dose group (P <0.01 or P <0.05),and serum content of IgE and IL-6 significantly lower in all the TCMgroups (P <0.01 or P <0.05).High-dose and mid-dose of TCM re-lieved EOS infiltration of nasal mucosa,compared with model group (P <0.01 or P <0.05),further-more,in TCMhigh dose group,no exposure-related nasal lesions or no flammatory cell infiltration was found (P <0.05).Conclusion TCM could improve the behavior of guinea pigs with allergic rhinitis and reduce serum IgE and IL-6 levels,relieve the nasal mucosa pathological changes.