OBJECTIVE To investigate the correlative between anxiety and coping mode of parents of children with air way foreign bodies. METHODS Self-rating anxiety scale (SAS) and simplified coping style questionnaire (SCSQ) were used to investigate the anxiety level and coping mode among 212 parents of children with airway foreign bodies. RESULTS The SAS scores of parents of children with airway foreign bodies was considerably higher than China normal, there was significant difference between the two (P <0.01), the positive coping style score was higher (P<0.05), while the negative coping style score was lower than the normal (P <0.01). The SAS score was significantly and positively related to the negative coping style score (P<0.01), and not related to the positive coping style score (P>0.05). CONCLUSION The parents of children with airway foreign bodies have higher anxiety level than the norm, they often take an positive coping style.

BACKGROUND: For elderly patients with unstable intertrochanteric fractures, hip arthroplasty is mainly applied due to the degenerative body function and difficulty in reduction.OBJECTIVE: To investigate the effectiveness and safety of cemented versus cementless hip arthroplasty for senile unstable intertrochanteric fractures.METHODS: We are conducting a prospective, single-center, non-randomized, controlled clinical trial at the Harrison International Peace Hospital, China. Eighty-six elderly patients with unstable intertrochanteric fractures were enrolled and allotted to control (n=44) and experimental (n=42) groups, followed by treated with cemented and cementless hip arthroplasty, respectively. The follow-up time is 6 months. The primary outcome is good and excellent rate according to Harris hip scores at 6 months postoperatively. The secondary outcomes are Harris hip scores and hip morphology on X-ray at 1, 3 and 6 months postoperatively, intraoperative blood loss, operation time, blood transfusion volume,time of off-bed and drainage volume at 1 month postoperatively, as well as the safety based on the incidence of adverse events at 6 months postoperatively. Partial results show that the blood transfusion volume and drainage volume postoperatively in the experimental group were significaintly higher than those in the control group (P < 0.05).There were no significant differences in the good and excellent rate according to Harris hip scores, Harris hip scores and incidence of adverse events between two groups at 6 months postoperatively (P > 0.05). There were four cases of bone cement implantation syndrome in the control group. The study protocol has been approved by the Ethics Committee of Harrison International Peace Hospital of China, approval number 2013-37. All protocols will be performed in accordance with the Ethical Principles for Medical Research Involving Human Subjects in the Declaration of Helsinki. Written informed consent was provided by each patient and their family members after they indicated that they fully understood the treatment plan.DISCUSSION: Cases were collected in January 2014 and the trial will be finished in December 2017. Cemented and cementless hip arthroplasty are mainly used to treat senile unstable intertrochanteric fractures. This study is designed to investigate the differences between cemented and cementless hip arthroplasty, thus providing referece for the treatment of unstable intertrochanteric fractures in the elderly. Partial trial results indicate that both cemented and cementless hip arthroplasties exhibit good curative efficacy and safety for senile unstable intertrochanteric fractures, while the occurrence of bone cement implantation syndrome in cemented hip arthroplasty should never be neglected.

Objective To explore the changes of serum leptin level in patients with diabetic microangiopathy.Methods A total of 90 subjects were assigned to three groups which included 30 healthy controls,30 diabetic patients without microangiopathy and 30 patients with diabetic microangiopathy.The studing groups were matched for sex and BMI.The level of serum leptin ,ⅣC,insulin and glucose of these patients were measured,and the correlation between serum leptin level and other indexes was analyzed.Results The level of serum leptin in controls,diabetic patients without microangiopathy and patients with diabetic microangiopathy were (7.20?2.11) ?g/L, (7.95?3.78)?g/L and (10.26?4.37)?g/L respectively .The patients with diabetic microangiopathy had higher serum leptin levels than in the diabetic patients without microangiopathy( t =2.18, P

Objective To investigate the elationship between the expression of Cathepsin D and Laminin receptors with the invasion and metastasis of gastric cancers.Methods Cathepsin D and Laminin receptors immunoreactivities were evaluated in 96 patients with gastric cancer using streptavaclin pemxidase method.Results Over expression of Cathepsin D in gastric cancer tissue was significantly related with the depth of invasion,lymphnode metastasis and grade of differentiation (P<0.01,P<0.01 and P<0.05, respectively).Moreover,the high expression of Lamninin receptors in gastric cancer had significant correlations with the invasion depth and grade of differentiation(P<0.05 and P<0.01);The positive expression of Laminin receptors were not significantly related with lymph node metastasis(P>0.05).By statistics analysis Cathepsin D had a significant positive correlation with Laminin receptors (P<0.05). Conclusion Cathepsin D and Laminin Receptors play all important role in the invasion and metastasis of gastric Cancers.

Objective To investigate the characteristics of Wallerian degeneration (WD) of secondary pyramidal tract after supratentorial cerebrovascular diseases, and to study the relationship between secondary WD and clinical performance. Methods We retrospectively reviewed and investigated the results of brain CT films of 272 cases suffered from supratentorial cerebral vascular diseases during 2001 till 2004. The number of supratentorial disorders was counted respectively in WD group and non-WD group, the distribution feature of these disorders was analyzed, and the changes in the cerebral peduncles of the midbrain and Activities of Daily Living Scale (ADLs) were determined. The information obtained was analyzed statistically respectively. Results Seventy-four cases of secondary WD were found, the rate of WD outbreak was 27.2%. In WD group, the ipsilateral cerebral peduncle of supratentorial disorder was significantly narrower than contralateral cerebral peduncle (P

Objective To evaluate the relationship between carotid arteriosclerosis and ischemic cerebral vascular disorders (ICVD). Method We examined 126 ICVD patients and 126 non-ICVD patients in the alterations of carotid dimension and intramembrane morphology with magnetic resonance angiography (MRA). Results Carotid plaques to various extent were detected in 82 cases of ICVD patients and 38 cases of non-ICVD patients. The incidence of carotid plaques was 68.3% and 35.7%, respectively in these two groups of patients. Furthermore, patients either with age over 60 years, hypertension, or hyperlipemia were found to be more susceptible to carotid arteriosclerosis. Conclusion MRA is very useful to detect early-stage carotid arteriosclerosis, and the occurrence in the carotid arteriosclerosis is closely related to ICVD.

Objective To investigate the effects of 2.45 GHz microwave on the apoptosis of HeLa cells and explore the underlying mechanism.Methods HeLa cells were divided into five groups randomly:control group,sham-irradiation group,and three irradiation groups exposed with 10,15 and 20 mW/cm2 2.45 GHz microwave for 10 min.Cell survival was tested by MTT assay,cell nucleus fashion was observed by a laser scanning confocal microscope,cell cycle distribution was measured by flow cytometry,and cell protein expressions were detected by Western blot.Results The cell survival in control group,shamirradiation group and low dose irradiation group had no difference but it became lower in the middle and high dose irradiated group(t =-16.47,-14.23,P ＜ 0.05).The abnormal rates of nuclear fashion in the low,middle and high dose irradiation groups were obviously higher compared with control group (t =9.37,11.25,8.47,P ＜ 0.05),while high dose irradiation group was higher than the low and middle dose group (t =15.32,10.25,P ＜0.05).The rate of HeLa cells in sub-G1 in the irradiation groups were higher than that in control group (t =15.24,22.31,10.69,P ＜ 0.05).The results of immunohistochemistry showed that the expression of Bax and Caspase-3 in irradiation groups was significantly increased,and the expression of Bcl-2 was decreased.Conclusions 2.45 GHz microwave can induce HeLa cells apoptosis with a positive correlation with microwave dose.

Objective To investigate the expressions of inflammatory factors in papillary thyroid carcinoma tissue and their relationships with the clinical pathological characteristics,and to provide an experimental basis for guiding clinical treatment and prognosis.Methods 74 patients with papillary thyroid carcinoma dignosed by puncture were selected as experimental group,and 26 cases of healthy people were used as normal control group.The fasting venous blood samples from the subjects in two groups were collected,and the postoperative specimens from cancer tissue,adjacent normal tissue and the fasting venous blood 7 d after operation of the patients in experimental group were collected again.The interleukin 1α(IL-1α),interleukin 1β(IL-1β)and cyclooxygenase-2 (COX-2)protein expression levels in serum,cancer adjacent normal tissue and cancer tissue were detected by ELISA;the gene and protein expression levels of IL-1α,IL-1βand COX-2 were examined by real-time fluorescent quantitative method and immunohistochemistry;the relationships between their expressions and clinical stages, pathological types,lymph node metastasis of thyroid papillary carcinoma were analyzed.Results The expression levels of serum IL-1α,IL-1βand COX-2 protein of the patients in experimental group were significantly higher than those in normal control group (P<0.01);the gene and protein expression levels of IL-1α,IL-1βand COX-2 in thyroid papillary carcinoma tissue were significantly higher than those in adjacent tissue (P<0.01).There were positive correlations between the expressions of IL-1αand IL-1β,IL-1αand COX-2,IL-1βand COX-2 in thyroid papillary carcinoma tissue (r=0.64,P=0.035;r=0.71,P=0.042;r=0.69,P=0.038).Conclusion The expression levels of IL-1α, IL-1βand COX-2 in thyroid papillary carcinoma tissue are increased, suggesting that inflammation is involved in metastasis and invasion of thyroid papillary carcinoma,its application is expected to become a new means of cancer treatment strategies.

Objective:To investigate the inhibitory effect and mechanism of different doses of Xiao Chai Hu Tang on C6 glioma cells cultured in vitro. Methods:C6 glioma cells were inoculated in 96 holes,24 holes and 6 holes,each culture plate was divided into 4 groups:control group, low dose group, middle dose group and high dose group, when the cells covered the bottom of culture plate 80%-90%,began adding,cultured for 24 hours after the ter mination of training. Cell proliferation activity,cell viability,protein content and protein positive expression intensity of VEGF and ESM-1,cell apoptosis in early and late stage were detected by CCK-8,in vivo staining,ELISA, immunocytochemistry and flow cytometry. Results: CCK-8 assay showed that the growth of C6 glioma cells was inhibited by low,medium and high dose group;ELISA and immunocytochemistry showed that the expression of VEGF and ESM-1 was lower in the lower, middle and high dose groups, and the levels of protein expression and protein levels were decreased. The flow cytometry showed that the low dose of small radix,middle and high dose group could promote the cell apoptosis. Inverted microscope ob-servation showed that with the increase of dose,the number of cells increased gradually,and the number of dead cells increased,and all kinds of detection methods showed that the inhibition effect increased with dose and dose dependence. The difference between the two groups was statistically significant(P<0. 01). Conclusion:The growth of C6 glioma cells was significantly inhibited by Xiao Chai Hu Tang. It may play a role in inhibiting tumor growth by down regulating ESM-1 and VEGF protein level and promoting cell apoptosis.

Objective To investigate the effect of acupuncture decompression operation on the quality of life of patients with mild to moderate essential hypertension. Methods 74 cases of mild to moderate essential hypertension were divided into experimental group and control group by random number table, 4 cases were lost. Finally, a total of 70 patients were included in the data analysis. In the control group, 35 cases were given routine drug taking and health guidance, the experimental group of 35 patients increase the treatment of acupuncture decompression operation points on this basis. The two groups of patients were measured with blood pressure before and after intervention, and World Health Organization Quality of Life (WHOQOL-BREF) was used to evaluate the quality of life, and the data were analyzed. Results The scores of physiological and psychological fields in the experimental group were (15.23 ± 2.66) points and (16.68 ± 2.23) points while the control group were (13.98 ± 2.18) points, (15.25 ± 2.52) points, the difference was statistically significant (t=2.305, 2.522, P<0.05). The experimental group of social relations, environmental score were respectively (1.37 ± 2.24) points, (14.16 ± 1.24) points, while the control group were (13.29 ± 2.30) points, (13.56 ± 1.36) points, 2 groups were not statistically significant (t=0.770, 3.063, P > 0.05). The experimental group systolic blood pressure, diastolic blood pressure were (121.43 ± 6.43) mmHg (1 mmHg=0.133 kPa), (71.74 ± 3.88) mmHg, compared with the control group were (128.34±5.72) mmHg, (76.06±4.43) mmHg. The difference was statistically significant (t=-4.753,-4.210, P<0.05). Conclusions acupuncture decompression operation can improve blood pressure in young and middle-aged patients with hypertension, improve the quality of life.