Eighty percent of unexplained syncope in children is neurally mediated syncope (NMS).The cur-rent clinical diagnosis of children NMS mainly depends on the head -up tilt test(HUTT),which has a certain risk, therefore searching for a safe noninvasive NMS diagnosis method is of great significance.Twenty -four -hour ambulato-ry blood pressure monitoring (ABPM)is widely used in clinic recently.It was reported that there was autonomic nerve adjustment imbalance in children with NMS.And the diagnostic value of ambulatory blood pressure pattern to NMS chil-dren was high.Non -spoontype blood pressure variation pattern of NMS could aid to diagnose children NMS.Fur-therly study of 24 h ABPMof NMS children has great significance to explore the pathogenesis of the NMS,to be benefi-cial to explain the regulating mechanism of the vessels,and go a step further to guide the treatment.

The common rheumatic diseases in children including rheumatic fever,juvenile idiopathic arthritis,systemic lupus erythematosus (including neonatal lupus),juvenile dermatomyositis,Kawasaki disease,anaphylactoid purpura.Rheumatic diseases as a group of unknown etiology autoimmune diseases,connective tissue and collagen fibers of different organs can be affected.Systemic inflammatory is the common prominent characteristics of this kind of disease,often resulting in multiple organ damage.Over recent decades,there has been considerable interest in the long-term outcomes of individuals with chronical inflammatory disease and an area of particular concern has been the increased prevalence of cardiovascular disease.Since sustained systemic inflammation is known to accelerate atherosclerosis,doctor should pay attention to rheumatic diseases associated cardiovascular involvements during daily clinical work.The cardiovascular involvement of common rheumatic diseases in children is summarized in this paper.

Objective To set up a stable method to induce SW872 adipocyte to differentiation.Methods THE animals were randomly divided in to five groups according to different inducing agents and a control groups.The changes of the cells were observed by microscopy and Oil-Red O staining.TG mass of these cells were assayed by chemical colorimetry methods.Results The oleic acid is the strongest factor to induce SW872 cell to differentiate,IBMX+DEX+Insulin is also strong factor,but it took a longer time to act.The effect of IBMX is stronger than insulin and ASP,but less than Oleic acid and IBMX+DEX+Insulin.Oleic acid significantly increased triglyceride mass of SW872 cell on the 1~(th) day of differentiation,The TG mass in the oleic acid group increased on the 2~(th) and4~(th)day of differentiation. Conclusion Oleic acid is the best Inducer to stimulate SW872 cell to differentiate,it can induce SW872 preadipocytes to differentiate to adipicytes in a short term.

Objective To study the genetic polymorphisms of STR locus D20S85 and to obtain the genetic data of Guangdong Han, Guangxi Zhuang, Huadong Han,Neimenggu Han and Meng populations. Method Using PCR and silver - staining methods, the alleles of D20S85 locus were detected and analysed. Results 9 alleles and 35 genotypes were found in those populations. The highest allele frequency was D20S85* 6. The results of Hardy - Wein-berg equilibrium test showed that the genotype distributions observed in five populations were correspondent with the expected. The expected heterozygosity was 0.7720 - 0.7912; the exclusion probability of paternity was 0.7538-0.7594(triplet) and 0.3988 -0.4297 (biplet) ; the discrimination power 0.9175 -0.9272 and the polymorphism information content 0.7442 - 0.7656. Conclusion STR D20S85 locus is a useful genetic marker for forensic application .

Objective To investigate the STR typing discordance between different typing methods.Methods Genotypes of 13 routine forensic STR loci in DNA samples from 100 individuals were typed by using singleplex polyacrylimide gel electrophoresis silver stain system and Power Plex16 System,respectively.The typing results between these two systems were compared.Results One genotype discordance was observed at D8S1179 locus in a DNA sample.The genotypes was 12/14 in snigleplex system and 12/15 in Power Plex16 System.Conclusion Different STR typing systems may result in different genotyps from the same DNA sample.

Objective To investigate the polymorphism of DXS6854 locus in Guangdong Han population. Methods The DXS6854 locus was analyzed by PCR following polyacrylamide gel electrophoresis and silver staining. Results Among 189 females and 230 males from Guangdong Han population, 8 alleles were observed with frequencies ranging from 0.0026 to 0.4522. Exact tests demonstrated genotype frequencies in females had no departure from Hardy- Weinberg equilibrium. The discrimination powers for female and male original samples were 0.8633 and 0.7012, respectively. When both mother and her daughter were tested, the probability of excluding a random man as a father was 0.6712. Conclusion The DXS6854 locus is appropriate for individual identification and paternity testing involving a female child.

After a description of the status quo of information literacy education in domestic colleges and universi-ties and the information literacy courses offered in University of Illinois according to The framework for information literacy in higher education, the information literacy courses offered in University of Illinois according to The frame‐work for information literacy in higher education were analyzed in aspects of their teaching target, teaching contents and teaching methods, with suggestions put forward for domestic information literacy education, including construc-tion of information literacy courses system and implementation of flexible teaching methods.

Objective To study the effects ofYiqi Huoxue Jiedu Recipe on immunomodulatory of patients with advanced ovarian cancer.Methods It was performed in a single-arm, open-label study with 40 patients with advanced epithelial ovarian cancer. All patients were treated withYiqi Huoxue Jiedu Recipe lasting for 4 weeks (200 mL, twice a day). The main endpoint was the quantities of CD4+CD25+ cells, CD8+CD28- regulatory T cells and CD8+CD28+ cytotoxic T cells measured by flow cytometry, and the accessory endpoints were variations of symptoms which included pain, fatigue, and numbness etc.Results The quantities of CD4+CD25+ and CD8+CD28- cells significantly decreased compared with those of pre-treatment (P<0.05); the quantity of CD8+CD28+ cell increased significantly (P<0.05). Except for pain, significant improvement showed in fatigue, tinnitus, numbness, poor appetite, abdominal discomfort, insomnia, diarrhea and abnormal urinate (P<0.05).ConclusionYiqi Huoxue JieduRecipe is proved to be effective in regulating immunosuppression advanced ovarian cancer and improving patients’ life quality.

Objective To identify routine forensic samples by genotyping method for HLA - A locus. Method A two - step PCR - SSP method was established. The first step is amplification with a pair of primers specific for all HLA - A alleles, the second step is amplification with primers specific for HLA - A30, A31, A33 respectively, using the first step amplification product as template. Secondly amplified PCR products were genotyped by electro-phoresis. Results 100 blood stains with a serological typing result of HLA - A30, A31 and A33 were tested with this method. The discrepancy rate between serological and genetic typing was 29%. Seminal stains, salivary stains reserved for 2 years and blood stains reserved for 18 years in room temperature gave satisfactory results. Conclusions It is better to replace serological typing by genetic typing. A two - step PCR - SSP genotyping method can be applied to forensic samples.

Objective To study the primer design for Penta D and Penta E genotyping and investigate the genetic polymorphisms of these two loci in Chinese Han population in Wuhan.Methods 281 unrelated Chinese individuals living in Wuhan were typed by hot-start PCR with re-designed Penta D and Penta E genotyping primers and PAGE technique,and the results were compared with those by the PowerPlex TM 16 system of Promega.Results The amplified fragment size of Penta D and Penta E loci with re-designed primers ranged between 153～198bp and 107～212bp respectively,which were consistent with the results obtained by the re-designed primers and PowerPlex TM 16 system,and the re-designed primers had a higher sensitivity than PowerPlex TM 16 system (0.2ng vs 0.5ng) by silver staining.10 and 21 alleles were observed for Penta D and Penta E in Chinese Han population in Wuhan,and the genotype distributions of the two loci were in accordance with Hardy-Weinberg equilibrium.Family studies confirmed Mendelian inheritance of alleles.The power of discrimination (DP) for Penta D and Penta E were 0.926 2 and 0.986 0,and the power of exclusion (PE) were 0.665 1 and 0.832 5 respectively.Conclusion The re-designed primers for Penta D and Penta E genotyping are reliable.These two loci are highly polymorphic in Chinese Han population and can be used in forensic identification and paternity test.