Objective To study MR imaging diagnostic value of brain-stem cavernous angioma(BCA).Methods 9 cases of BCA proved both surgically and pathologically with integrated clinical,MRI and pathological data were analyzed.Results The clinical attacks were divided into two types:the sudden and the resting.The MRI findings of BCA included:isointensity or hypointensity on T1WI(76.5%),isointensity or hyperintensity on T2WI(85.3%),heterogeneous mixed signal intensity on both T1WI and T2WI (36.2%),hypointense ring round angioma on T2WI (16.2%).On Gd-DTPA scanning,the lesions were slight enhancement or no enhancement in 8 cases.The shapes of lesions were round-like in most cases(86.6%),and typical moruloid or network in some cases(11.7%).The lesions localized in the midbrain in 3 cases,in the pons in 4,and in the medulla oblongata in 2.The histopathological findings included:composite pathological changes in most cades,elastic fibers in the angiomatous lacuna vasorum in 3 cases,visible drainage vein and supply artery in 2 cases.MRA showed hyperintensity around the angiomas in 5 cases.Conclusion MR imaging is of significant value in diagnosing BCA.

Objective To study the hydrogen sulfide productivity by mononuclear cells in patients with atrial fibrillation (AF). Methods Mononuclear cells were extracted from peripherial blood of 60 AF patients and 30 healthy controls. Modified methylene blue method was used to evaluate the hydrogen sulfide productivity of mononuclear cells. Results Hydrogen sulfide productivity of mononuclear cells from AF patients was 92.5%higher than that from control [10.63 (8.21~14.37) μmol/(min.g protein) vs 5.52 (4.42~8.50) μmol/(min.g protein)]. Hydrogen sulfide productivity of mononuclear cells was correlated with the left atrial transverse diameter (r = 0.276,P < 0.01), while no significant correlation was found between hydrogen sulfide and left ventricular end-diastolic diameter , left atrial anterior posterior diameter , C-reaction protein etc. Conclusion Hydrogen sulfide productivity of mononuclear cells from AF patients is promoted.

Objective: To probe the effects of gross polysaccharide of Radix Rehmanniae Preparata(GPRRP) on the morphology changes of immune organ of blood deficiency model mice.Methods: Using the blood deficiency mice models induced by releasing blood and injection of Cyclophosphamide as the experimental objects,the model animals were divided into 5 groups,that was the large,middle and small dose of GPRRP groups,Danggui Buxue Oral Liquid group,model group,at the same time,giving a blank control group.It was given successively relating drugs to the animals of several groups for 10 days.At the end of the experiment,the hemogram was measured and the thymus and spleen were taken,and making pathological section,then these tissues morphology changes were observed under microscope.Results: GPRRP can obviously resist the atrophy of thymus and spleen induced by the model establishing,can obviously increase the depth of thymus cortex and the number of cortex cells,can enlarge the size of spleen nodules and obviously increase the number of lymphocyte.Conclusion: GPRRP can resist the atrophy of thymus and spleen in blood deficiency model mice.

Metabotropie glutamate receptor (mGluR) 2/3 plays an important role on the nociceptive transmission from periphery to spinal cord.The previous studies demonstrated that mGluR2 can contribute to mechanical hypersensitivity and thermal hypersensitivity in rat.Therefore,in the present study,by using the immunofluorescenee histochemical technique,we try to explore that whether mGluR2 is colocalized with acid-sensing ion channel 3 (ASIC3),a muhi-modulator of mechanosensation,or transient receptor potential/vanilloid receptor subtype-1 (TRPV1),which responses for thermosensation in dorsal root ganglion (DRG).Morphological observations showed that mGluR2-immunoreactivity was mainly distributed in cellular plasma of neurons in DRG.The counting number results indicated that 35.84% of DRG neurons were mGluR2-immunoreactive (ir).On the other hand,82.61% of mGluR2-ir cells were the small-diameter neurons (diameter:＜30 μm),5.79% of which were the medium-diameter neurons (diameter:30-50μm) and 11.59% of which was the large-diameter neurons (diameter:＞50 tun).Furthermore,42.45% and 79.78% of mGiuR2-ir cells was individually co-localized with ASIC3-or TRPVI-ir in small-diameter neurons in the double-labeled immunofluorescence sections.The present results suggest that mGhiR2 mainly exists in small neurons of the DRG,which are regarded as nociceptors consisting of AS-and C-fibers.While mGluR2 is highly co-localized with ASIC3 and TRPV1,implying their potential relationship in DRG may be involved in mechanical hypersensitivity and thermal hypersensitivity.

Anesthesiology rotation is an important part in resident standardization training of surgery. However, there are many problems in the teaching process. For example, the key points of teaching is not clear, the process of teaching is not coherent, and teachers and students lack interaction. The Rotation Record is introduced in order to solve those problems. The Rotation Record effectively reflects the fundamental process and major learning point of anesthesiology department which contains three items: the usage and recording of instrument; the communication and the basic knowledge and operating skills of anesthesia. The Rotation Record can initiate learning activity, emphasize important contents, supervise and urge teaching and improve the learning effect. By introducing Rotation Record, the assessment results in the surgical residency in anesthesiology has greatly improved compared to the previous ones, and has effectively improved the teaching quality of anesthesia.

Objective This study was aimed to characterize the swine leukocyte antigen( SLA) class I genes of GGTA1 -/ - Wuzhishan minipigs and compare their similarity to human leukocyte antigen( HLA) . It has important implica?tions for understanding the cellular rejection in xenotransplantation. Methods Specimens of ear tissue from six founding GGTA1 -/ - Wuzhishan minipigs were collected, and the SLA class I genes (SLA?1, SLA?3, SLA?2) were amplified by RT?PCR. Purified products were cloned into pEASY?T1 vectors and sequenced, followed by BLAST alignment and using bioin? formatc analysis to characterize the SLA class I genes and compare with the similarity to HLA. Results A total of six al?leles were detected, among them alleles were previously reported (SLA?1?0703,SLA?2?1102, SLA?3?0401, SLA?3?0403), and the other were novel (SLA?1?0401wz01, SLA?2?11wz01). The homology between alleles of SLA class I genes in Wuzhishan minipigs and HLA was from 70?5% to 72?1%. The homology analysis of critical amino acid residues on HLA binding with human CD8 + molecules showed that SLA?1?0401wz01, SLA?1?0703, SLA?2?11wz01, SLA?2?1102 and SLA?3?0401 occurred mutant at amino acid positions 225 and 228 ( T→S,T→M) , whereas the other loci were highly conserved. There was a high homology at amino acid level between SLA?2?11wz01, SLA?2?1102 and HLA class I genes which are NK cell KIRs binding sites. Conclusions The amino acid sequences of SLA class I genes of GGTA1 -/ -Wuzhishan minipigs have a high homology to HLA. From the point of view of cell?mediated xenograft rejection, the amino acid sequences of SLA class I genes of GGTA1 -/ - Wuzhishan minipigs have a high homology to HLA, therefore, Wzhishan minipigs may become a good potential donor for pig?human xenotransplantation.

Objective To investigate the association of serum interleukin-6 and interleukin-18 concentration in patients with meta-bolic syndrome (MS), and explore the role of inflammtory markers in these patients. Methods 83 patients with MS were enrolled into study group, among of them, 47 were male and 36 were female, their average was 48.95±10. 17 years. The control group was made of 85 normal volunteers. The levels of fasting blood glucose (FBG), fasting insulin, plasma lipids, interleukin-6 and interleukin-18 were meas-ured in all the patients and normal subjects. The insulin sensitive index was calculated. The levels of the fasting blood inflammatory markers were compared between the two groups. Results Compared with the control group, the HOMA-βof the MS group was significantly de-creased, but the levels of IL-18 and IL-6 were significantly increased. The serum IL-18 levels were positive correlated with FPG, FINS, TG, HOMA-IR, IL-6. Conclusion There are IR and abnormal serum level of IL-18 and IL-6 in patients with MS. It has an intimate association between abnormal serum IL-18 and IR. The inflammatory reaction may play an important role in the development of IR in patients with MS.

The stable quality of Chinese herbal medicines is a critical factor for their reliable clinical efficiency. An improved liquid-liquid extraction procedure and a liquid chromatographic method were developed to simultaneously analyze five anthraquinones (aloe-emodin, rhein, emodin, chrysophanol and physcion) in a Chinese traditional hospital preparation, Fuyankang mixture, in order to quantitatively control its quality in a more effective way.

OBJECTIVE: To explore diagnosis and surgical treatment of symptomatic lumbar spinal epidural lipoplasia. METHODS: A retrospective analysis of 19 patients with symptomatic lumbar spinal epidural hyperplasia treated with hemilaminectomy and interbody fusion and internal fixation from February 2012 to November 2018 were performed, including 7 males and 12 females, aged from 48 to 72 years old with an average of (57.6±1.2) years old;the course of disease ranged from 6 to 60 months with an average of (18.6±5.1) months;plane requiring decompression:L RESULTS: All patients were followed up from 12 to 37 months with an average of (16.3±3.8) months. Ninteen patients were successfully completed operation, and all adipose tissues in the compressed segment of the spinal canal were removed. Operation time was from 125 to 260 min with an average of (186± 15) min, and blood bleeding was from 150 to 500 ml with an average of (280±46) ml. Two patients occurred partial incision fat liquefaction and exudate did not heal, the incision was opened to remove effusion, the dressing was changed and anti-inflammatory treatments were performed. No complications such as cauda equina injury, cerebrospinal fluid leakage, and broken nails occurred. Preopertaive VAS of back pain and leg pain were 5.3±0.7 and 6.8±0.8, respectively, while 2.1±0.4 and 2.3±0.5 respectively at 6 months after opertaion, there were statisticalsignificant difference between 6 months after operation and before operation ( CONCLUSION Patients with symptomatic lumbar spinal epidural lipoplasia undergo hemilaminectomy and internal fixation of compression segment could relieve compression of dura mater and cauda equina, and achieve good clinical results.
Back Pain ; Child, Preschool ; Female ; Humans ; Infant ; Lumbar Vertebrae/surgery* ; Male ; Retrospective Studies ; Spinal Fusion ; Treatment Outcome

OBJECTIVE: To investigate the feasibility and clinical effect of hemi-resection of posterior arch of atlas in the upper cervical spinal dumbbell-shaped schwannomas. METHODS: A retrospective analysis was performed on 13 patients with high level cervical dumbbell schwannomas from January 2005 to December 2018, including 10 males and 3 females, aged 19 to 67 years old. The occipital foramen to the C RESULTS: The operation was successfully completed in 13 cases of this group. No vertebral artery injury or spinal cord injury occurred during the operation. All 13 patients were followed up for more than 12 months. No local recurrence was found. Both the VAS and the JOA score were significantly improved compared with those before surgery. The ASIA classification before operation was:1 case of grade C, 6 cases of grade D, 6 cases of grade E;the latest follow up was 3 cases of ASIA grade D and 10 cases of E. CONCLUSION The posterior arch of the atlas hemisection can remove the upper cervical dumbbell schwannoma in one stage. The short-term clinical effect is good, and there are no complications such as cervical instability.
Adult ; Aged ; Cervical Vertebrae ; Female ; Fracture Fixation, Internal ; Humans ; Male ; Middle Aged ; Neoplasm Recurrence, Local ; Neurilemmoma ; Retrospective Studies ; Treatment Outcome ; Young Adult