Nematoda anticoagulant protein c2((NAPc2)) is a protein originally isolated from the canine hookworm Ancylostoma caninum.NAPc2 binds to a noncatalytic site on fⅩa then selectively inhibits fVⅡa/TF complex.NAPc2 is proven to be a safe and potent anticoagulant in Phase Ⅰ and Phase Ⅱ clinical trials,and it has prolonged elimination half-life(longer than 50 h).In a word,NAPc2 may play a promising role in the prevention and treatment of thrombosis,disseminated intravascular coagulopathies(DIC) and tumor.

Objective To investigate the effects of progesterone on apoptosis and Survivin expression protein of Ishikawa cell line of human endometrial carcinoma in vitro.Methods The proliferation of Ishikawa cells in vitro was determined by methyl thiazolyl terazolium (MTT) medhod.Cell cycle,apoptotic percentage were detected by cytometer. The expression of Survivin protein of Ishikawa cells was detected by immunocytochemistry. Results Progesterone inhibited the growth of Ishikawa cells significantly when the concentration of progesterone reached at 0.5?10-5～10-4mol/L(P

Objective To evaluate the expression of annexin V in maternal blood and placenta,and to explore the relationship between annexin V and preeclampsia(PE). Methods 120 women with PE who delivered babies in the Second Hospital of Hebei Medical University from December 2007 to December 2009 were chosen as study groups. They were classified into four groups: early-onset mild group (n =30),early-onset severe group (n=30), late-onset mild group ( n = 30) and late-onset severe group (n=30). 30women without perinatal complications who accepted elective term cesarean section were chosen as control group. Western blot and immunohistochemistry were used to detect the expression and localization of annexin V in placenta and maternal blood. Flow cytometry was employed to detect the apoptosis of cytotrophoblast.Annexin V mRNA level was determined by reverse transcription (RT)PCR. Prothrombin time (PT),activated partial thromboplastin time (APTT), fibrinogen (FiB), international normalized ratio (INR) were detected in each group. Results (1) The expression of annexin V in placenta and maternal blood were:0.54±0.12 and 0.62±0.17 in early-onset mild group; 0.47±0.15 and 0.56±0.24 in early-onset severe group; 0.74±0.23 and 1.08±0.32 in late-onset mild group; 0.68±0.28 and 0.72±0.21 in late-onset severe group;1.73±0.35 and 1.55±0.27 in control group. They were significantly lower in PE groups than in control group (P＜0.05 ). However, there was no significant difference among PE groups (P＞0. 05). (2) The early apoptosis, late apoptosis percentage of trophoblast cells were: 3. 21%, 0. 86%, in early-onset mild group; 5.32%, 0. 72%, in early-onset severe group; 2. 43%, 0. 63%, in late-onset mild group;4. 28%, 0. 48% in late-onset severe group; 1.05%, 0. 59%, in control group. Early apoptosis percentage in each group of PE was higher than that in control group ( P ＜ 0. 05 ). However, there was no significant difference among PE groups (P＞0.05). (3) The annexin V mRNA levels in placenta were:25.0±3.0 in early-onset mild group; 24. 8 ± 3.0 in early-onset severe group; 25.4 ± 3. 9 in late-onset mildgroup; 25.1±2.7 in late-onset severe group, respectively. All were significantly higher than that in control group (30. 6±3.0, P＜ 0. 05), and no significant difference was found among PE groups (P＞0.05).(4) PT, APTT, FiB, INR levels were: (11.3±2.4), (25.6±2.9) s, (4.6±0.9) g/L and 0.9 ±0.2in early-onset mild group; (12.1±1.9), (27. 2 ±2. 1 ) s, (5.0 ± 1. 0) g/L and 0. 9 ±0. 2 in early-onset severe group; (11.7±2.3), (26.5±2.3)s,(5.0±0.7)g/L and 0.8±0.3 in late-onset mild group;(11.4±2.6), (27.3±3.0) s, (4.3 ±0.8) g/L and 0.8 ±0.3 in late-onset severe group; (12.4±2.7), ( 28.0±1.9) s, (5.1±1.2) g/L and 0.9 ± 0.2 in control group. There was no significant difference among PE groups and control group ( P ＞ 0.05 ). Conclusion The expression changes of annexin V in placenta and maternal blood were observed in patients with PE. This indicated that annexin V played an important role in the pathogenesis and progression of PE by affecting coagulation.

Objective To learn current status of elderly depressive disorders in Yuetan community residents to provide evidence for policy making.MethodsCluster sampling method was used to enroll 352 elderly individuals from Yuetan Community for Geriatric Depression Scale(GDS)and self-designed questionnaire.Results The morbidity of elderly depressive disorder was 23.86%(84/352).The awareness rate of risk factor of elderly depressive disorder Was 4.83%to 42.90%.Conclusion The morbidity of elderly depressive disorder of Yuetan Community is higher,and related healthcare education should be strengthened.

Objective To compare curative effects between laparoscopic oviduct-sparing operation and medical interventional treatment for the management of tubal pregnancy.Methods A total of 63 patients with tubal pregnancy were randomly divided into two groups.One group underwent laparoscopic salpingotomy and aspiration of the gestational sac content, while the other group underwent intra-tubal injection of methotrexate(MTX).Curative effects of the two procedures were compared.Results Laparoscopic oviduct-sparing operation was successfully performed on one session in 31 out of 32 patients,the success rate being 96.9%.Medical interventional treatment was conducted successfully in 24 out of 31 patients,23 of whom received one dose of MTX and 1 of whom received two doses,the success rate being 77.4%.There was no statistical difference between the two groups in success rates (?~2=3.765,P=0.052).The time for serum ?-hCG to normal levels was significantly shorter in patients receiving laparoscopic oviduct-sparing operation(9.1?2.1 d) than in patients receiving interventional treatment(26.3?3.9 d)(t=-20.974,P=0.000).Conclusions Both laparoscopic oviduct-sparing operation and medical interventional treatment can effectively preserve affected oviduct for patients with tubal pregnancy.As compared with medical interventional treatment,laparoscopic oviduct-sparing operation has a broader range of indications,less side effects,and a shorter recovery time of serum ?-hCG.

Objective: To examine the relationship between parental rearing and adolescent personality traits. Methods: A sample of 637 adolescents was tested by CAPI and EMBU. Results: Neuroticism of girls is higher than that of boys, but interpersonal harmony, face and defensiveness of boys is higher than those of girls. Extraversion, openness and agreeableness of only child is higher than those of other adolescents. Most correlations between parental rearing and Adolescent personality traits are significant. Parental warmth and understanding have positive effect on adolescent extraversion, agreeableness and conscientiousness, and father punishment and mother refusal have negative effect on adolescent agreeableness and interpersonal relationship. Conclusion: Adolescent personality is greatly affected by parental rearing.

Objective To study the operating approaches of laparoscopic resection of ovarian benign cystic teratomas. Methods 67 patients underwent operative laparoscopy for ovarian cystic teratomas(23 ovarian cystectomies,44 oophorectomies or adnexectomies),including 27 patients who had cysts more than 8cm in diameter.In operation, a plastic bag was used and the abdominal cavity was abundantly flushed with warm physiologic saline.The larger cysts were aspirated to reduce the cysts and extracted through abdominal wall incision. Results Cyst rupture occurred in 17 cases (17/44) when cystectomy was performed. No cyst spillage occurred in all the oophorectomies and adexectomies.The mean operating time was (34?19)min.Blood loss was 3ml～7ml.No complications occurred and none of the patients needed conversion from laparoscopy to laparotomy.chemical peritonitis wes not seen in any of the patiants. Conclusions Laparoscopic surgery is safe and feasible for ovarian benign cystic teratomas and allows the removal of larger ovarian cysts.

Objective:Comprehensive evaluation on the puerperal women's health status,and probing those influencing factors on their quality of life. Methods:513 puerperal women received the survey on health status including physical, psychological and social function dimensions by relevant items of Quality of Life designed by WHO and related factors were analyzed by stepwise regression.Results:The QOL of puerperal women were lower than general women aged between 18-49 years old in three dimensions(91.44?4.05/97.84?3.66,u=25.95,82.42?7.02/89.81?8.50,u=17.53,88.87?4.99/98.12?4.59,u=29.47,均P

Objective To compare the efficacy between microwave endometrial ablation(MEA) and abdominal hysterectomy(AH) in the treatment of abnormal uterine bleeding(AUB).Methods A total of 80 patients with AUB was divided into two groups,either receiving MEA(40 patients) or receiving AH(40 patients).The clinical efficacy,operation time,intraoperative blood loss,white blood cell(WBC) count,and neutrophil granulocyte rate(NGR) before and after operation were compared.Results The effective rate for abnormal uterine bleeding was not significantly different between MEA(97.5%,39/40) and AH(100%,40/40)(?2=0.000,P=1.000).As compared with AH,MEA was characterized by a shorter operation time(4.8?1.2 min vs 102.5?34.6 min;t=17.848,P=0.000) and less intraoperative blood loss(0 ml vs 138.3?40.6 ml;t=21.544,P=0.000).Conclusions Microwave endometrial ablation is a novel and efficient minimally invasive method for abnormal uterine bleeding.

Objective To investigate the value of thorough uterine curettage in microwave endometrial ablation(MEA).Methods Preoperative uterine curettage was conducted in 126 women with abnormal uterine bleeding(AUB)scheduled for MEA from June 2001 to June 2005.At the same period,another 12 patients with AUB underwent hysterectomy.And before the operation,uterine curettage and subsequent MEA were conducted.Then the uterine specimens were histopathologically and histochemically examined for evaluating the tissue damages.Results No intraoperative complications occurred in the 126 patients.Postoperatively,amenorrhea was observed in 78 patients(61.9%),normal menstruation was achieved in 46 patients(36.5%),and small amount of irregular vaginal bleeding was found in 2 patients(1.6%),the overall satisfaction rate being 98.4%(124/126).Postoperative complications included endometritis in 10 patients,hematometra in 2 patients,and dysmenorrhoea leading to hysterectomy in 2 patients.Follow-up observations were conducted for 6～28 months(22?4 months)in 126 patients,116 of whom were followed for more than 2 years.Follow-up findings showed satisfactory surgical effects.After MEA,the injury depth was 4.2～4.9 mm in the uterine fundus,4.1～5.7 mm in the anterior wall,4.7～6.6 mm in the posterior wall,and 4.0～4.7 mm in the uterine horn.Conclusions MEA with preoperative thorough uterine curettage can completely destruct the endometrium.The method offers good efficacy,high satisfactory rate,and low risk of tissue injury.