Objective To investigate the effects of humanistic care on primiparas' role competency during pregnancy and delivery.Methods Data of 21 cases were collected using semi-structured interviews and phenomenological qualitative method was used to analyze the data.Results The effects of humanistic care on maternal role competency during pregnancy included:to participate in related curriculum,to get ready for delivery actively.The effects of humanistic care on maternal role competency during delivery included:nurses' carefulness gave psychological support,nurses' instruction gave professional support,nurses' encouragement gave spiritual power,nurses' support enhanced belief,successful delivery experience gave.satisfaction.Conclusions Humanistic nursing is helpful to improve delivery experience,to increase maternal satisfaction and confidence,to promote matemal role competency and harmonious relationship between mothers and nurses.

Objective:To give the test in cases of changing the conditions of measurement and reference value, compare the test results, and detect the capacity of QuickCheck, so as to offer reference for the daily morning check of accelerator and the evaluation of test results. Methods:Change the output dose, field size, SSD, angle of collimator and gantry angle of the accelerator, and then evaluate QuickCheck’s capacity of monitoring accelerator accordingly. Compare the field parameters measured by QuickCheck with the data from the 3D water tank. Results:When the output dose was 1 cGy higher than the reference value or 6 cGy lower than it, the field size expanded or contracted more than 2 cm simutaneouly, SSD was 2 cm higher or 1 cm lower than reference value, the collimator rotated more than 2 °, the gantry angle rotated more than 6 °, and then the test results of QuickCheck surpassed threshold. Comparing the measuring results of QuickCheck with 3D water tank, the difference between the flatness of the two was 3.84%, symmetry (G/T) 0.67%, symmetry (L/R) 0.47%and dose rate 1.2 MU/min. Conclusion:QuickCheck is rather accurate in testing the output dose, field size, angle of collimator, flatness, symmetry and dose rate, so it could meet the requirement of accelerator’s daily QA. However, its capability is relatively poor on examining SSD and the deviation of gantry angle.

0.05).Conclusion The V allele in exon24 of A2M might be one of the susceptible factors in PD in Shanghai Hans.

1. Microsurgical relationships of the arteries at the base of the brain and the trigeminal nerve were studied bilaterally on 30 brains. Twenty seven trigeminal nerves made contact with the arteries 33 times which represents 55 percent of the cases. Six of 27 trigeminal nerves had double contact.2. Of 33 arteries in contact with 27 nerves, seventeen involved the superior cerebellar arteries (SCA) (28.33?5.82%), seven the anterior inferior cerebellar arteries (AICA) (11.67?4.15%) and nine the middle inferior cerebellar arteries (MICA) (15.0?4.61%).3. The outer diameter of the superior cerebellar artery is the largest averaging 1.41?0.20 mm; the anterior inferior cerebellar artery comes next averaging 1.06?0.36 mm and the middle inferior cerebellar artery the smallest, averaging 0.62?0.14 mm.4. 27 trigeminal arteries arising from the upper or middle segement of the basal artery were found on the ventral surface of the trigeminal nerves in 17 brains, and 16 trigeminal branches arising from the AICA. MICA. and PICA were also found at the roots of 16 trigeminal nerves. Anastomoses were noted between the trigeminal arteries and the trigeminal branches in four cases of 16 trigeminal nerves.5. The association between the neurovascular contact and the trigeminal neuralgia are discussed. It is suggested that the neurovascular contact either from dorsal or ventral aspects may be the anatomical cause of the trigeminal neuralgia.6. The neurovascular contact of the trigeminal nerve with the SCA was usually on the dorsal or dorsalmedial aspect of the nerve, but the point of contact with AICA or MICA was mainly on the ventral aspect of the nerve. Besides, there are trigeminal arteries and trigeminal branches at the ventral surface of the nerve. All these topographic observations provide microsurgical information for neurosugeons.

Objective To investigate the effect of Shuanghuanglian injection parafornix-injecting in treating uterine tube obstruct (UTO) and explore its mechanism. Methods Clinic study included 386 uterine tubes of 200 patients, which were randomly divided into treatment group (50 cases, treated with Shuanghuanglian injection parafornix-injecting) and three control groups - group one (50 cases, treated with Lincocin vial parafornix-injecting), group two (50 cases, treated with hydrotubation) and group three (50 cases, treated with transvaginal catheter dilation). The changes of UTO condition and blood rheology, IL-2, IgA, IgG and IgM were observed before and after treatment. Results The total effective rate of Shuanghuanglian injection in treating UTO was 97.76%. It was characterized by regulating the blood stasis and hyperviscosity, improving the immune function of human body. Conclusions Shuanghuanglian injection parafornix-injecting has obvious therapeutic effect on UTO. The effect may be related with rectifying the body pyrotoxin and blood stasis, improving pelvic blood circulation and immune function.

This paper analyzed the importance and urgency of clinical research of HIV/AIDS and discussed the
present research situation and bottleneck of HIV/AIDS clinical research . The application and advantages of Chinese medicine in the clinical research of HIV/AIDS had been listed . And it summarized the Chinese medicine research results on HIV/AIDS management . It also suggested the outlook of Chinese medicine in the HIV/AIDS clinical research .

Objective To construct the recombinant RIP3 over-expressed plasmids and transfect them in breast cancer MCF7 cells, and identify the expression and localization of fusion protein, as well as its effect on the death way of MCF7 cells. Methods The expression levels of RIP3 mRNA in four breast cancer cell lines and normal mammary epithelial cells were detected by reverse transcription polymerase chain reaction (RT-PCR). The RIP 3 coding sequence was amplified by polymerase chain reaction and subcloned into mCherry vector to construct recombinant plasmids. The plasmids were transfected into MCF7 cells by lentivirus after DNA sequencing, then screened by basticidin (4 mg/L) for 1 week. The efficiencies of RIP3 expression were validated by Western blotting assay. The death way of mCherry-RIP3-MCF7 cells was observed under the treatment of TNF-αand Z-VAD-FMK. Results The lowest expression of RIP3 mRNA was found in MCF7 cells. The sequencing results validated the well recombinant plasmids. The expression of mCherry-RIP3 fusion pro-tein with a molecular weight of 85 ku was detected by Western blot assay. The mCherry-RIP3 expression enhanced the sensi-tivity of MCF7 cells to TNF-αand Z-VAD-FMK induced cell death. Conclusion The recombinant RIP3 over-expressed plasmids were successfully constructed, and the stable MCF7 cells with ectopic RIP3 transfection were obtained. The mCher-ry-RIP3 fusion protein was expressed in the cytoplasm and was conformed to mediate TNF-αinduced necroptosis.

According to the basic theory of traditional Chinese medicine (TCM), the pathogenetic factors such as platelet activation, adhesion, congregation and thrombosis fall into the category of blood stasis, while the pathological changes such as tissue necrosis, oxidative stress injury and inflammation, etc, are far beyond the etiological category of blood stasis. The toxin or the combination and transformation of toxin and blood stasis of TCM are involved in the pathogenesis of thrombotic cerebro-cardiovascular diseases. It is significant to recognize and stress the combination and transformation of toxin and stasis in pathogenicity so as to enrich TCM etiology and improve TCM clinical efficacy in the treatment of cerebro-cardiovascular and thrombotic diseases.

Huntington disease (HD) rat model was produced by injection of ibotenic acid (IA) into the head of right caudate putamen (CP). One month post lesion, fetal (El5-17) striatal cell suspension was implanted into the lateral ventricle ipsilateral to the lesion, and the rats were devided into four groups; group I normal control rats (n=8), group II model control rats (n=10), group III simple transplanted with fetal striatal cell suspension, (n=10, ST group), group IV grafted with striatal cell suspension containing laminin (n=6, LST group). Three and six months after grafting, active avoidence test was carried, the results showed that there were significant differences bewteen grafted groups and model control group at three and six months, and no significant differences between LST group and normal group either in three or six months whereas between ST group and normal group no significant difference only can be found at six months. Overnight locomotor activity was measured in each group at six months post grafting, the results indicated that the locomotor behaviour of model control group was hyperactive whereas the overnight hyperactivity was compensated completely in the grafted groups. After the behavioural test, the rat brain was investigated morphologically. The head of the lesioned CP was atrophied and the graft was located at the dorsal part of the atrophied CP and projected into the lateral ventricle. The volume of the graft area of LST group was larger than that of ST group. ChAT, GABA and Leu-ENK positive neurons were found in the graft area of the two grafted groups and their shape and size were similar to those of nomal CP. The processes of AChE positive neurons in the graft of LST group were more and longer than that in the graft of ST group. The results indicated that the fetal striatal neurons implanted into the lateral ventricle of HD model rats not only can survive and grow well but also ameliorate the behavioural deficits of the IA lesioned rats and the laminin may supports the neuronal suvival and growth in vivo.

Objective To analyze the factors influencing fiducial number used for tracking abdominal tumor with stereotactic body radiation therapy (SBRT),which was implemented by CyberKnife.Methods 254 abdominal tumor patients chosen which adopted fiducial tracking and classified the fiducials.We gathered data of the patients with ＜ 3 fiducials used for tracking and patients with ≥ 3 fiducials respectively,and analyzed the reason why ＜ 3 fiducials were left for tracking.Results Among 254 patients,22 patients were showed that the number of fiducial implantation were equal to play number of fiducial;232 patients with no equation which 49 patients (21.1％) used ＜ 3 fiducials for tracking,183 patients (78.9％) used ≥3 fiducials for tracking.Among the 49 patients with ＜3 fiducials for tracking,9 patients had fiducials migrated,1 patient had an unrecognizable fiducial,23 patients had the implantation not qualified for tracking,and 16 patients had the rigid error surpassing the threshold.Conclusions 78.9％ patients were implanted ≥ 3 fiducials and thus qualified for fiducial tracking.Major reasons of disqualification include insufficient implanted fiducials,unqualified implantation and rigid error surpassing the threshold.