Asymmetric dimethylarginine(ADMA) is an endogenous inhibitor of nitric oxide synthases(NOS),which is a risk marker of cardiovascular endothelial dysfunction.Recent studies found that ADMA was a predictor not only for acute cardiovascular disease,but also for critical illness and mortality.Studies have found that exogenous ADMA altered lung function and induced apoptosis in animal experiments and vitro experiments.The levels of plasma ADMA significantly increased in children with hypoxia,PPHN,brain damage,and preterm infants who required mechanical ventilation.ADMA could be a independent risk factor to predict neonatal complications and death.

Objective To investigate the influence of LNG-IUS placement on endometrial thickness, recurrence rate and complications of patients with endometrial polyps and moderate anemia after TCRP. Methods 140 patients with endometrial polyps and moderate anemia were chosen from January 2014 to January 2016 and randomly divided into control group (70 patients) with TCRP used alone and observation group (70 patients) with LNG-IUS placement after TCRP; the endometrial thickness, hemoglobin levels and PBAC score before and after treatment, the recurrence rate and the complication incidence of both groups were compared. Results The endometrial thickness after treatment of observation group were significantly less than control group before the treatment (P < 0.05). The hemoglobin levels and PBAC score after treatment of observation group were significantly better than control group before the treatment (P < 0.05). The recurrence rate of observation group were significantly lower than control group (P < 0.05). There was no significant difference in the complication incidence between the two groups (P > 0.05). Conclusion LNG-IUS placement in treatment of patients with endometrial polyps and moderate anemia after TCRP can efficiently reduce the endometrial thickness, decrease the uterine bleeding amount, prevent the long-term recurrence and not increase the complications incidence.

Mild cognitive impairment (MCI) is considered as an intermediate clinical state between normal aging and Alzheimer’s disease (AD). The cognitive function and life ability of patients are normal, which have not met the criteria of AD. MCI is considered to be an earlier stage of AD. The clinical effects of AD in the middle or late stage are unsatisfactory. Therefore, studies on MCI have become the hot research areas both at home and abroad. The therapy of MCI is similar to AD. The treatment effect is uncertain. Hence, the understanding and treatment of MCI by TCM reflected TCM features. This article reviewed on TCM understanding of MCI, TCM syndrome differentiation criteria and TCM treatment methods of MCJ. Thus, it was aimed to further understand the dynamic of MCI in order to provide references for improving TCM clinical effects and reducing the incidence of AD.

Objective To assess the effect of fructose sodium diphosphate and alpha-lipoic acid on diabetic peripheral neuropathy. Methods Sixty-six patients with diabetic peripheral neuropathy were randomly divided into two groups, additional to the regular treatment to control blood glucose, 34 patients in the treatment grouptreated with fructse sodium diphosphate 10 g and alpha-lipoic acid 0.45 g daily through intravenous drip for two weeks (3 patients dropped out due to un-tolerance), 32 patients in the control group treated with alpha-lipoic acid 0.45 g dai-ly through intravenous drip for two weeks. All patients were examined on the improvement of symptoms, motor and sensory nerve conduction velocity of the median nerve and nervus peroneus communis before and after the treatment. Results After treatment for two weeks, the overall effect rate in the treatment group was 93.55 % (28/31), which was significantly higher than that in the control group(68.75%,22/32) (P < 0.05). In the treatment group, the motor nerve conduction velocity for the median nerve and nervus peroneus communis was 47.7 (SD:3.8) m/s and 41.6 (SD: 3.3) m/s after treatment, which was significantly higher than that before treatment (42.5 [SD:2.5] and 36.0[SD:2.8] m/s,respectively) (P <0.05). In the control group, the motor nerve conduction velocity for the median nerve and nervus peroneus communis was 43.4 (SD: 2.0) m/s and 39.6 (SD: 3.3) m/s after treat-ment, which was significantly higher than that before treatment (41.9 [SD16.0] and 36.2 [SD: 1.9] m/s, respec-tively) (P < 0.05). In the treatment group,the sensory nerve conduction velocity for the median nerve and nervus peroneus communis was 40.3 (SD: 1.8) m/s and 34.1 (SD: 2.3) m/s after treatment, which was significantly higher than that before treatment (35.2 [SD:2.3] and 28.8 [SD:2.5] m/s,rospectively) (P <0.05). In the control group,the sensory nerve conduction velocity for the median nerve and nervus peroneus communis was 37.7 (SD:1.7) m/s and 30.8 (SD:2.6) m/s after treatment,which was significantly higher than that before treatment (34.8 [SD :3.1] and 27.8 [SD :2.4] m/s, respectively) (P < 0.05). Furthermore, the improvements in the treat-ment group was significantly higher than those of the control group (P < 0.01). Conclusions Fructse sodium si-phosphate combined with alpha-lipoic acid is more effective to treat diabetic peripheral neuropathy than alpha-lipoic acid only.

Pulse-taking is the most specific diagnostic method of TCM.Its significance has been recognized by ancient and modern doctors.However,at present the application and spread of pulse-taking is limited.In this paper,the author made detailed explorations of literatures and studies the value of pulse condition in guiding syndrome differentiation of liver diseases.The study shows abnormal pulse in left guan and taut pulse in cunkou location both suggest the liver is disordered.We can judge the location of diseases through pulse condition.This exploration shows pulse-taking plays important role in syndrome differentiation.But there is one thing should be paid more attention,pulse condition should be integrated with other symptoms,that will be reliable in guiding diagnosis.

Objective To investigate the differential expression of microRNAs(miRNAs) in transplanted kidney undergoing chronic rejection by the technique of RNA microarray.Methods Four biopsy specimens from transplanted kidney undergoing chronic rejection were harvested as test group(CR),and 3 biopsy specimens were obtained from normal renal cortex as normal control group(NC).Total RNA of each sample was extracted using Trizol reagent.miRNAs were isolated and differential expression of miRNAs were screened by miRNA array analysis.The results of miRNA array were validated by RT-PCR.The quantity and quality of all the RNA samples were checked by gel electrophoresis and absorbance at A260/280,respectively.Results It was confirmed that the isolated RNA was of appropriate quality.The results of miRNA array analysis showed that there were 63 differential expression miRNAs in CR group,of which 35 were up-regulated and 28 down-regulated.There were 9 differential expression miRNAs which distributed in 3 gene clusters: 14q32.31,22q11.21 and xq27.3.The miRNAs hsa-miR-637,hsa-miR-648 and hsa-miR-516-5p were randomly selected for relative quantification by real-time PCR.It was showed that the expression ratios of hsa-miR-637,hsa-miR-648 and hsa-miR-516-5p in AR/NC,when detected by RT-PCR,were 0.034,2.670 and 7.846,while the ratios were 0.035,2.660 and 7.857 when analyzed by miRNAs array.The results from two methods were not significantly different,so the method of miRNAs array was reliable.Conclusions It is notable that the differential expression of miRNAs existed in the transplanted kidney undergoing chronic rejection.miRNAs might be helpful in protecting the patients undergoing kidney transplantation against chronic rejection.

Objective To explore the influence of plasma homocystine(Hcy) level on the prognosis of acute cerebral infarction(ACI) and the effect of interventional therapy.Methods The plasma Hcy levels of 152 patients with ACI were measured by fluorometric method at

Objective To investigate the distribution features of upper limb involved nerves in patients with diabetic peripheral neuropathy (DPN).Methods The sensory nerve conduction velocity (SCV)and amplitude(SNAP),motor nerve conduction velocity(MCV)and amplitude(CAMP)of median,ulnar and radial nerves were examined in 98 DPN patients (DPN group) and 32 normal controls(NC group).The distributive ferture of involved upper limb nerves in DNP patients were analysed.Results (1) Comparing to NC group,the SCV and MCV of median,ulnar and radial nerves in DPN group were significantly slower (P

Objective To explore the expression level of CD4+ CD25+ CD127low-regulatory T cells (Treg) in the peripheral blood of hepatitis B virus (HBV)-infected individuals,and its association with liver pathology.Methods Thirty-five HBV-infected individuals and 20 healthy controls were enrolled.The frequencies of peripheral blood CD4+ CD25+ CD127low/-Treg were detected by flow cytometry.HBV-related serological tests as well as ultrasound-guided liver biopsies were performed in HBV-infected individuals.The two groups were compared by t test.Spearman and Pearson correlation analyses were performed.Results The alanine aminotransferase (ALT) level of HBV-infected individuals was (34.57 ± 15.36) U/L,with 25 subjects of ALT＜40 U/L.Twenty subjects were hepatitis B e antigen (HBeAg) positive,and 15 were HBeAg negative.The frequency of the peripheral blood CD4+ CD25+ CD127low/-Treg in HBV-infected individuals was (5.59 ±1.50)％,which was significantly higher than that in healthy controls [(4.85±0.95) ％ ; t=2.191,P=0.033].The level of peripheral blood CD4+ CD25-CD127low/-Treg was positively correlated with HBV viral load (r=0.495,P=0.003).Thirty-one subjects had liver inflammatory score ≤2,with a peripheral blood CD4+ CD25+ CD127low/-Treg level of (5.80 ± 1.44) ％,which was significantly higher than that of the rest 4 subjects who had liver inflammatory score ＞2 [(4.00±0.77)％; t=2.425,P=0.021].Twenty-four subjects had liver fibrosis score ＜2,with a peripheral blood CD4+ CD25+ CD127low/-Treg level of (6.00±1.44) ％,which was significantly higher than that of the rest 11 subjects who had liver fibrosis score ≥ 2 [(4.70 ± 1.24)％; t =2.559,P＝0.015].The expression of CD4 + CD25 +CD127low/-Treg in peripheral blood was inversely correlated with liver inflammation (r=-0.539,P=0.001) and fibrosis (r =-0.488,P =0.003) in HBV-infected individuals.Conclusions CD4+ CD25+ CD127low/-Treg is associated with chronic condition and liver damage of hepatitis B.It may play an important role in occurrence,development and prognosis of the disease.

Objective To observe the effect of plasma exchange (PE)therapy for removing serum interleukin-6 (IL-6),tumor necrosis factor alpha (TNFα),and interferon gamma (IFNγ)in patients with liver failure,and to assess the value of artificial liver support system in the treatment of liver failure.Methods The study included 36 patients with liver failure who were hospitalized in the First Affiliated Hospi-tal of Bengbu Medical College from February 2012 to February 2013.Blood samples were collected before PE and the next morning after mul-tiple treatments.Plasma TNFα,IL-6,and IFNγlevels were measured by ELISA.Between-group comparison of measurement data before and after treatment was made by independent-samples t test,and comparison of mean values between groups was made by analysis of vari-ance.Results All the 36 liver failure patients had significantly lower levels of TNFα(381.23 ±190.57 ng/L vs 274.12 ±212.30 ng/L,P<0.05),IL-6 (77.9 ±83.09 ng/L vs 54.8 ±63.32 ng/L,P<0.05),and IFNγ(534.65 ±471.19 ng/L vs 259.65 ±312.26 ng/L,P<0.05)after treatment than before treatment.According to the severity of clinical manifestations,the patients with subacute or acute-on-chronic (subacute)liver failure were divided into early stage,middle stage,and advanced stage groups.There were significant differ-ences in IL-6 and TNFαdecreases between the early stage,middle stage,and advanced stage groups (P<0.05).IFNγdecrease signifi-cantly differed between the early stage or middle stage group and advanced stage group (P<0.05),but not between the former two groups (P>0.05).After PE therapy,29 out of 36 liver failure patients recovered well,while the remaining 7 patients deteriorated.The recovered group had significantly greater decreases in TNFα(122.58 ±57.64 ng/L vs 42.45 ±19.86 ng/L,P<0.05),IL-6 (26.93 ±7.25 ng/L vs 6.71 ±3.55 ng/L,P<0.05),and IFNγ(284.06 ±94.31 ng/L vs 217.47 ±45.76 ng/L,P<0.05)than the deteriorated group.The response rates of patients with acute,subacute,acute -on -chronic,and chronic liver failure were 66.67%,85.71%,86.36%,and 50%,respectively.Conclusion Continuous artificial liver PE therapy can effectively remove pro-inflammatory cytokines from plasma and thus improve the clinical outcomes of patients with liver failure.