The etiology of congenital heart disease (CHD) incluses genetic factors, environmental factors and maternal factors. The genetic etiology of CHD includes chromosomal abnormalities, single gene defects and multiple genetic defects. The genetic testing method of CHD has made great progress in karyotype analysis,fluorescence in situ hybridization, quantitative PCR, multiplex ligation dependent probe amplification, et al.

Objective To establish a HPLC method of determining chrysin and baicalein in Senmen Oroxyli. Method The contents of chrysin and baicalein were determined by HPLC. The chromatographic conditions were: Kromasi1-C_(18) column (5 ?m, 250 mm?4.6 mm), with methanol-acetonitrile-water-glacial acetic acid (25 : 25 : 50 : 0.2) as the mobile phase, at the flow rate of 1 mL/min, the detecting wavelength at 275 nm and at the room temperature. Results The contents of baicalein and chrysin could be detected by this HPLC method, and got good separation and good linear relationship. RSD were 1.53% and 1.35%, indicating good repeatability, recovery RSD were 1.85% and 1.59%, indicating a good recovery. Conclusion The method is simple, feasible and repeatable, and can be provide reference for quality control of Senmen Oroxyli.

Objective To understand the children′s development status of deciduous teeth erupt,fontanel closure,expressive language and gross motor in Zhengzhou.Methods According to random cluster sampling,the children health care clinic in three hospitals in Zhengzhou were investigated,all the data was gathered from the chil-dren health care management information system.4 230 healthy children aged 0-3 years old birth from 2000 to 2010 were investigated(2 141 boys and 2 089 girls),We collected the following information about birth situation,the dynamic physical growth,deciduous tooth occurrence,language and motor development.Results (1 )The children's weight,height has been persistent and significantly improved during the past 10 years in Zhengzhou.(2)There was a significant difference between the sexes in deciduous teeth erupt and the fontanel closure time.(3 )With the time, infant motor development and fontanelle closed slightly ahead of time.Conclusion The children's weight,height growth shows a long-term trend in Zhengzhou,with normal condition in neuropsychological development,and presents a slightly ahead of the trend.

To investigate the effect of enhancing hospital infection management in new medical personnel on the im -provement of the level of hospital infection management .Comprehensive management was performed in new medical personnel through ideological education , refining infection control procedures , strengthening training and assessment , combining theory with practice , and reward and punishment , etc.Through enhancing hospital infection management in new medical personnel , the overall awareness of hospital infection prevention and control in the medical personnel is improved , and thereby effectively reducing the occurrence of hospital infection .

Objective To observe the efficacy and safety of Sanhuang gancao mixture combined with antibiotics in the treatment of children with purulent meningitis.Methods 52 cases with purulent meningitis were randomly and double-blindly divided into observation group and control group of 26 cases in each group.The control group received ceftriaxone sodium treatment, observation group received Sanhuang gancao mixture combined with ceftriaxone sodium therapy.The tumor necrosis factor (TNF-α), high-sensitivity C-reactive protein (hs-CRP) and white blood cell (WBC) levels in cerebrospinal fluid and dispearance time of related symptoms, therapeutic effect and adverse reactions in two groups were observed before and after treatment.Results After treatment, the TNF-α, hs-CRP and WBC levels in cerebrospinal fluid of observation group were lower than the control group (P<0.05).The time returning to normal temperature, time of consciousness recovery, time of cerebrospinal fluid returning to normal, dispearance time of intracranial hypertension were shorter than control group (P<0.05).The total efficacy in observation group was higher than control group(P<0.05). There existed adverse reactions in two groups but had no significant difference.Conclusion Sanhuang gancao mixture combined with antibiotics could reduce the inflammatory, return white cells to normal and accelerate the recovery process of sign whose efficacy is better than antibiotics alone without increasing the adverse reactions in the treatment of children with purulent meningitis.

The pathogenesis of viral myocarditis is still not completely clear all over the world now.However,the damage to the body caused by the viral myocarditis has been highlighted and its pathogenesis has become a research hot spot.With the development of immunology and molecular biology,the pathogenesis of viral myocarditis has made great breakthrough.Many scholars advocate that the course of viral myocarditis should be divided into three phases:viral replication,immune response and inflammatory dilated cardiomyopathy.This review summarizes the progress of the pathogenesis of viral myocarditis from the above three phases through reviewing previous articles,aiming to provide new evidence and references for clinical diagnosis and therapy of viral myocarditis.

Objective To observe the clinical efficacy and adverse reactions of antibiotic in treating tubal infertility,which provide support in the clinical applicaiton.Methods 68 cases of tubal infertility caused by Uu and Ct were randomly divided into the control group and observation group according to random digital table.There were 34 cases in each group.The control group was taken hydrotubation.The observation group was given doxycycline 0.1g orally twice a day for 7 days and azithromycin according the results of drug sensitive test on the basis of the control group.In two groups mycoplasma and chlamydia positive rate,pregnancy,clinical efficacy and adverse reactions were compared.Results After 7 days of treatment,Uu positive rate in the observation group was 14.7%.Ct positive rate was 17.6%.Uu positive rate in the control group was 58.8%.Ct positive rate was 64.7%;Uu positive rate and Ct the positive rate in both group showed significant difference(χ2 =14.23,15.54,all P<0.05).The total effective rate in the observation group was 85.3%.In the control group,the total effective rate was 58.8%.The rate in the two groups showed significant difference(χ2 =5.91,P<0.05).Adverse reactions occurring rate in the control group was 17.6%,and the incidence rate of adverse reactions in the observation group was 26.4%.The rate of adverse reactions had no significant difference between the two groups(χ2 =0.77,P >0.05).Conclusion Antibiotic in treating patients with tubal infertility showed significant clinical efficacy and little side effects.We could draw a conclusion that this program was worthy of further clinical application and study.

OBJECTIVE:To establish the quality standard of Sophora flavescens decoction.METHODS: The preparation was determined qualitatively using TLC,and the contents of matrine and oxymatrine were determined by HPLC.RESULTS: The TLC spots of S.flavescens decoction were clear and well-separated.The linear ranges of matrine and oxymatrine were 0.024 92～0.648 44 ?g(r=0.999 9) and 0.073 4～1.908 4 ?g(r=0.999 9).The average recoveries were 102.9% and 102.3% and RSDs were 6.9% and 102.3%,respectively (n=6).CONCLUSION: Establish standard can be used for the quality control of S.flavescens decoction.

ObjectiveTo study the influence of health education on the quality of life among patients with mammary cancer. Methods Divided 83 patients with mammary cancer into the recovery group (45 cases) and the control group (38 cases) randomly. The health education was used in the recovery group and the routine nursing method was used in the control group, and then using the scale to evaluate the quality of life for the two groups. Results The quality of life in the recovery group was significant better than that of in the control group,P

MIC.CONCLUSION:Oxacillin should be administrated 3 or 4 times a day with intravenous drip infusion duration prolonged to at least 3 hours per time.