1.Construction and identification of infectious full-length cDNA clone of enterovirus 71
Chinese Journal of Microbiology and Immunology 2010;30(9):809-815
Objective To construct an infectious full-length cDNA clone of enterovirus 71(EV71)and develop a technological platform for study on vaccine development as well as molecular virology of EV71.Methods According to the nucleotide sequence of EV71 strain 085 isolated in China,four pairs of primers were designed for amplification of four end to end overlapping subgenomic cDNA fragments,the cDNA fragments were directional cloned into pBluescript SK(+)vector,and the virus genome cDNA clone was obtained by ligation orderly.The rescued virus of parental strain 085 from RNA transfected host cells was identified by RT-PCR,IFA,titration as well as transmission electron microscope(TEM)after the transcription of the full-length cDNA clone in vitro.Results The full-length cDNA clone was constructed successfully,and the typical CPE was observed after its transcription into Vero cells.The rescued virus with 20-30 nm in diameter can not only be neutralized by EV71 special anti-serum but also react with anti-EV71 monoclonal antibody that virus infected cells stained with FITC can be detected by IFA.After amplification from the total RNA extraction of virus infected cells by RT-PCR with EV71 special primers,the 226 bp products can be detected.The growth curve showed that the rescued virus can propagate in Vero cells stably with a titer of 4.5 ~6.0 lgCCID50/ml during 8 passages.The plaque formed by rescued virus is identical as parental virus in morphology but smaller in size.Conclusion An infectious full-length clone of EV71 was developed successfully,which will be used for further study on pathogenesis and vaccine development of EV71.
2.Hyperuricemia and kidney diseases
Chinese Journal of Applied Clinical Pediatrics 2014;29(17):1284-1287
The morbidity of hyperuricemia(HUA) has showed an increasing tendency in recent years,along with the improved social living conditions.HUA has been proved to be closely related to kidney diseases,metabolic diseases and cardiovascular diseases.However,people pay little attention to the relationship between uric acid and renal diseases in pediatrics,and the relevant studies and progress are lacking.Now a review is made about the metabolism of uric acid,the etiology and pathogenesis of HUA,and the relationship between HUA and kidney diseases or drugs,in order to pay attention to the relationship between HUA and kidney diseases.
3.Research progress of application of immunotherapy by chimeric antigen receptor-engineered T cells
Cancer Research and Clinic 2015;27(2):139-141
The technique of chimeric antigen receptor-engineered T cells (CAR-T) is combined single chain antibody and T-cell activation modification and has specific and durable killing effect on tumors.It had achieved stunning results on clinical trials of B lymphocytic leukemias,B lymphomas and solid tumors.Meanwhile it also had potential risk on the off target effects,cytokine release syndrome,graft versus host disease and so on.This paper briefly reviews the principal of CAR-T cellular mechanism,advantages and clinical applications.
4.Advantage Analysis of Qilian Fuzheng Capsule in Maintenance Treatment of Advanced Non-small Cell Lung Cancer
Huijie LI ; Yuanfu QI ; Xiurong LI
Chinese Journal of Information on Traditional Chinese Medicine 2014;(3):21-23
Objective To observe the effect and analyze the advantage of Qilian Fuzheng capsule in maintenance treatment of advanced non-small cell lung cancer. Methods The advanced non-small cell lung cancer patients were randomly divided into observation group and control group, 32 cases in each group. The control group was given the best supportive care, and the observation group was given Qilian Fuzheng capsule 4 capsules three times daily additionally. The tumor control rate, quality of life, PFS and OS of the two groups were compared by follow-up. Results Qilian Fuzheng capsule maintenance treatment of advanced non-small cell lung cancer improved the quality of life. PFS of observation group and control group was 5.4 months and 4.1 months, respectively (P<0.05), and OS of the two groups was 12.3 months and 10.6 months, respectively (P>0.05). The tumor control rate of observation group was 77.42% (24/31), control group was 60.00% (18/30), with no significant difference (P>0.05). Conclusion Qilian Fuzheng capsule is a feasible application in maintenance treatment of advanced non-small cell lung cancer.
5.Clinical characteristics and survival analysis of 368 cases of smoking lung cancer patients
Xiurong LI ; Huijie LI ; Dikui XU
Journal of International Oncology 2013;(4):309-312
Objective To discuss the clinical characteristics and the relationship with the survival of smoking lung cancer patients.Methods The clinical data of 368 cases were retrospectively analyzed by comparing the differences of the age,gender,histological type,clinical stage,treatment and tradition Chinese medicine typing,and their correlation with the survival was analyzed.Results Among the 368 patients,the ones aged between 50 to 69 accounted for 69.84% ; the proportion between the male ones and the female ones was 7.98 ∶ 1 ; the proportion of the squamous cell carcinoma was as high as 52.45% ; the proportion of the patients with small cell lung cancer in extensive stage was significantly higher than that of the patients in the limited stage(75.38% ∶ 24.62%) ; the numbers of the patients with non-small cell lung cancer in stage Ⅲ and Ⅳ was significantly higher than that in the stage Ⅰ and Ⅱ (82.77% ∶ 17.23%) ; the ones with non-surgical treatment accounted for 76.36%,and the proportion of ones with surgery treatment was 23.64% ; most of the patients were treated by both Chinese medicine and chemotherapy(41.04%) ; the main types of all patients were qi-yin deficiency syndrome and toxic heat flourishing syndrome,accounting for 36.96% and 29.28% respectively.The age (F =10.356,P =0.000),clinical stage (F =11.289,P =0.000),treatment methods (t =2.624,P =0.021),smoking index(F =3.681,P =0.021) were the key factors effecting the survival (P < 0.05),and the gender,histological type,tradition Chinese medicine type had no significant effect(P > 0.05).Conclusion The clinical characteristics of the cases are 50-69 aged,male,squamous cell carcinoma,advanced stage,treatment without surgery.The survival of smoking lung cancer patients is effected by the age,clinical stage,treatment methods and smoking index.
6.Clinical analysis of the therapy for recurrent and intractable non-small cell lung cancer with combination of cryoablation and intervention
Futong ZHANG ; Xiuli LI ; Huijie LI ; Yonghui AN
Journal of Interventional Radiology 2006;0(11):-
Objective To explore the re-treatment approach for nonsmall-cell lung cancer(NSCLC)in clinic with therapeutic alliance of cryotherapy and interventional chemotherapy. Methods Sixty two patients with uncontrolled NSCLC after common radiotherapy and(or)chemotherapy were re-treated with therapeutic alliance of cryotherapy and interventional chemotherapy, and their serum tumor markers of NSE, CEA, CY21-1 and the value(I.S%,I.O.D%)of correlative protein in nucleolus region-Ag-NORs were tested before and after the re-treatment. Meanwhile, patients' one year survival and therapeutic effect to NSCLC were recorded during the follow up. Results After the re-treatment, distinguished decline of the serum tumor markers was observed(P
7.Arsenic,Selenium Pollution in Capsicum and Corn Roasted by Coal-combustion in Zhaotong Fluorosis Areas and the Cumulation in Human Hair
Huijie LI ; Kunli LUO ; Xuezhi WU
Journal of Environment and Health 2007;0(07):-
Objective To investigate whether there was arsenic and selenium pollution besides fluorine pollution in the capsicum and corn roasted by coal-combustion in Zhaotong fluorosis areas were collected,and the cumulation in human hair. Methods In September 2005,the capsicum and corn samples were collected before they were roasted and after roasted by coal-combustion,and the hair samples from people living in Zhaotong fluorosis areas were collected,and the fluorine,arsenic and selenium content in these samples were determined. Results The average arsenic and selenium content in the fresh capsicum not roasted by coal-combustion were 0.034 mg/kg and 0.079 mg/kg respectively. The average arsenic and selenium content in the capsicum roasted by coal-combustion were 0.463 mg/kg and 1.088 mg/kg respectively. The average arsenic and selenium content in the fresh corn were 0.019 mg/kg and 0.051 mg/kg respectively. The average arsenic and selenium content in the corn roasted by coal-combustion were 0.058 mg/kg and 0.085 mg/kg respectively. Except for the average selenium content of the capsicum roasted by coal-combustion,others were all within the limits of arsenic and selenium in foods. Conclusion There is not only fluorine pollution in the capsicum and corn roasted by coal-combustion,but also arsenic and selenium pollution. Furthermore,the selenium pollution level was higher than that of arsenic pollution. The arsenic and selenium pollution level in capsicum was higher than that in the corn. Though this arsenic pollution in the capsicum and corn may be harmful for the people living on these capsicum and corn roasted by coal-combustion to some extent,it was not harmful enough to result in arsenism. Comparing with the arsenic pollution,the selenium pollution in the capsicum and corn may be beneficial for the people eating the capsicum and corn roasted by coal-combustion.
8.Discussion of Analytical Approach with Operation of Large and Medium-sized Medical Equipment
Huijie BAI ; Zhendong CAO ; Li ZHANG
Chinese Medical Equipment Journal 1993;0(06):-
Medical equipment is playing an important role in medical treatment, teaching and scientific research, especially in how to improve the utilization rate of large and middle medical equipment to ensure the safety and integrity of medical equipment in the clinical operation, and this is a very significant job. Through the operation of large and middle medical equipment to conduct analysis, comparison and statistics, it can be found the issue and adjusts timely to improve the medical equipment management of the technological level and economic benefits.
9.Reversion after total hip arthroplasty: a follow-up of 85 cases
Huijie LI ; Jie BAO ; Zimei GAO
Chinese Journal of Tissue Engineering Research 2017;21(23):3640-3645
BACKGROUND:With the prevalence of total hip arthroplasty,the demand for revision has been increased due to the prosthesis life and various complications.OBJECTIVE:To retrospectively analyze the reasons and clinical effectiveness of revision,clinical application of revision prosthesis,and related revision techniques.METHODS:Clinical data of 85 patients undergoing total hip revision were analyzed retrospectively.Among which,there were 23 cases of biological T.O.P acetabular cup (allograft bone graft) plus polyethylene liner,51 cases of titanium mesh cup plus bone cement fixation,and 11 cases of common acetabular cup;common hip stem in 63 cases (including 47 cases of bone cement fixation);lengthened hip stem in 34 cases (16 cases of modular prosthesis and 18 cases of bone cement fixation).Subsequently,the hip function was assessed by Harris hip scores.RESULTS AND CONCLUSION:All cases were followed up,and the mean follow-up was 30.2 months.(1) The average Harris hip scores changed from preoperative 30.07 to postoperative 90.32.The wound healed well,no dislocation or infection,and the hip function was improved significantly.(2) To conclude,aseptic loosening of the prosthesis is the main cause of reversion occurred,followed by prosthesis dislocation.Acetabular wall bone defect and decrease in bone mass are the main problems in revision.Adequate preoperative preparation for possible and unexpected events,and reasonable selection of prosthesis according to the bone defects will be helpful.Noticeably,early arthroplasty reversion will obtain good clinical efficacy.
10.Meta-analysis of influence of TNF-α and NSE levels in cerebrospinal fluid on children with viral encephalitis
Yan WANG ; Huijie HUANG ; Bin LI
Chongqing Medicine 2017;46(24):3366-3369
Objective To systematically evaluate the influence of TNF-α and NSE levels in cerebrospinal fluid on Chinese children with viral encephalitis (VE).Methods Literature about the influence of TNF-α and NSE levels in cerebrospinal fluid on Chinese children with VE,which were published before Nov.1,2016,were searched in databases including CNKI,Wanfang Data,Cochrane Library and PubMed.And the case-control study about the TNF-α and NSE levels in cerebrospinal fluid between Children with VE (VE group) and children without VE (control group) were included in this study,and the quality evaluation was implemented and the data were extracted.And then the RevMan 5.3 was used to carry out the meta-analysis.Results Nine case-control studies were included in this study,involving 536 cases in the VE group and 284 cases in the control group.The results showed that the level of TNF-α and NSE in cerebrospinal fluid of the VE group were all significantly higher than that of the control group,and the differences was statistically significant [SMD=1.75,95%CI:1.56-1.93,P<0.01;SMD=1.34,95%CI:1.17-1.51,P<0.01].Conclusion The levels of TNF-α and NSE in cerebrospinal fluid are positively correlated with the incidence of VE in Chinese children.