Objective To explore the effect of Cx43 protein on improvement of learning and memory ability induced by enriched environment (EE) in rats with traumatic brain injury (TBI). Methods TBI model was made by fluid percussion injury (FPI) in Sprague-Dawley rats. The TBI rats were divided into EE group (A), standard housing (ST) group (B), Cx43 specific antisense oligonucleotides (Cx43 ASODN)+EE group (C) and scrambled sequence ODN+EE group (D) with 6 rats in each group. Another 6 normal rats were taken as the control group. Groups C and D were given hippocampal microinjection of Cx43-ASODN (2 μl/d/rat, 1.5 mmol/L) and ScrbASODN (2 μl/d/ rat, 1.5 mmol/L) respectively. Morris water maze was used to evaluated the learning and memory ability. Results The latency was longer and the traversing times was less in group B than in the control group (P<0.05). The latency was shorter in group A than in group B (P<0.05), and there was no significant difference between group A and the control group (P>0.05) from the 9th day after injury. The traversing times was more in group A than in group B and there was no significant difference between group A and the control group (P>0.05). The latency was longer and the traversing times was less in group C than in group A (P<0.05). Conclusion Cx43 protein may participate in the improvement of the learning and memory ability induced by EE in rats with TBI.

Objectives. Dendro-dendritic and dendro-somatic projections are common between spinal motoneurons. We attempted to clarify whether there are functional connections through these projections.Methods. Motoneurons were antidromically stimulated by the muscle nerve and recorded intracellularly to examine the direct interaction between them, after the related dorsal roots had been cut.Results. Excitatory connections, demonstrated by depolarizing potentials in response to muscle nerve stimulation, were found between motoneurons innervating the same muscle or synergistic muscles, but never between motoneurons innervating antagonistic muscles. These potentials were finely graded in response to a series of increasing stimuli and resistant to high frequency (50Hz) stimulation.Conclusions.These results indicate that excitatory connections, with certain specificity of spatial and temporal distribution, occur in the spinal motoneurons. It is also suggested that electrical coupling should be involved in these connections and this mechanism should improve the excitability of the motoneurons in the same column.

Objective To prepare the water soluble chrysin-hydroxypropyl-β-cyclodextrins inclusion compound and widen the administration path of chrysin .Methods The cogrinding method had been used to prepare chrysin-hydroxypropyl-β-cyclodextrins in-clusion compound .The PXRD, DSC and IR techniques had been used to characterize the inclusion compound .Results Chrysin and hydroxypropyl-β-cyclodextrins had formed the inclusion compound , and the formation of the inclusion compound could increase solubili-ty by 120.7 times.Conclusion The inclusion compound preparation method was simple and available , which was suitable to improve the bioavailability .

Objective To study effect of refined nursing management in the treatment of asthma in children with acute bronchitis and its effect on lung function,white blood cell count and Creactive protein.Methods A total of 128 children with bronchitis admitted in our hospital were randomly divided into study group and control group,with 64 cases in each group.The patients in the control group were treated with traditional care,and the patients in the study group implemented refined nursing management.The symptom disappearing time,oxygen saturation,pulmonary function indicators [forced expiratory volume in one second (FEV1),forced vital capacity (FVC),peak expiratory flow rate (PEFR)],white blood cell count,and C-reactive protein were observed and compared in two groups.Results The symptoms disappeared time such as cough and asthma in the study group was significantly shorter,and oxygen saturation was higher than that in the control group (P ＜ 0.05).Before nursing intervention,levels of FEV1,FVC and PEFR between the two groups had no significant differences (P ＞ 0.05),and were significantly improved after nursing in both groups,and the study group improved more than that in the control group (P ＜ 0.05).After nursing intervention,the C-reactive protein and white blood cell counts in both groups were lower than before nursing,and the study group was lower than the control group,the difference was statistically significant (P ＜0.05).Conclusion Refined nursing management in children with acute bronchitis can significantly reduce asthma,cough symptoms,shorten treatment and hospitalization time,improve treatment compliance and lung function,so it is worthy of further promotion.

Objective To study effect of refined nursing management in the treatment of asthma in children with acute bronchitis and its effect on lung function,white blood cell count and Creactive protein.Methods A total of 128 children with bronchitis admitted in our hospital were randomly divided into study group and control group,with 64 cases in each group.The patients in the control group were treated with traditional care,and the patients in the study group implemented refined nursing management.The symptom disappearing time,oxygen saturation,pulmonary function indicators [forced expiratory volume in one second (FEV1),forced vital capacity (FVC),peak expiratory flow rate (PEFR)],white blood cell count,and C-reactive protein were observed and compared in two groups.Results The symptoms disappeared time such as cough and asthma in the study group was significantly shorter,and oxygen saturation was higher than that in the control group (P ＜ 0.05).Before nursing intervention,levels of FEV1,FVC and PEFR between the two groups had no significant differences (P ＞ 0.05),and were significantly improved after nursing in both groups,and the study group improved more than that in the control group (P ＜ 0.05).After nursing intervention,the C-reactive protein and white blood cell counts in both groups were lower than before nursing,and the study group was lower than the control group,the difference was statistically significant (P ＜0.05).Conclusion Refined nursing management in children with acute bronchitis can significantly reduce asthma,cough symptoms,shorten treatment and hospitalization time,improve treatment compliance and lung function,so it is worthy of further promotion.

The functional cure of hepatitis B means that patients have good clinical outcomes, which is the ideal endpoint of hepatitis B antiviral treatment. Whether chronic hepatitis B patients can achieve functional cure is related to the nature of the virus itself, such as virus species, virus replication and expression of related proteins, on the other hand, it is also related to the host body's immunity, including innate immunity and specific immunity. Immune factors play a vital role in the functional cure of hepatitis B. Antiviral drugs, including interferon, often play an antiviral role by regulating immunity. This paper reviews the relationship between hepatitis B and functional cure from the perspective of immunity, in order to provide the basis for the clinical pursuit of functional cure, hoping to provide guidance for clinical practice.

Objective:To explore the status of HBV infection, immune response to hepatitis B vaccine and its influencing factors of one-year old children born to HBV infected mothers in real world.Methods:In this cross-sectional study, eligible mothers infected with chronic HBV and children who completed standard vaccination against hepatitis B vaccine and hepatitis B immunoglobulin injection were selected. Clinical biochemical, virological measurements, data of antiviral therapy and complications during pregnancy and childbirth were collected by HIS(Hospital Information System)system and LIS (Laboratoty Information Management System) system. At one year of age, the children were tested for HBsAg, HBsAb and HBV DNA in venous blood specimens.Results:A total of 1 302 eligible mothers and children were collected, including 600 in high viral load group (mothers’ HBV DNA≥2×10 5 IU/ml) and 702 in low viral load group (mothers’ HBV DNA<2×10 5 IU/ml). In high viral load group, 587 patients received antiviral drugs in the middle or late trimester (Treated group) and 13 patients did not receive antiviral drugs (Untreated group). No chronic HBV infection occurred in children of low viral load group, and in 5 cases (0.83%) the infection occurred in high viral load group. In the five HBV infected children, 3 cases (0.51%) were in the treated group, and 2 cases (15.38%) in untreated group. The failure rate of mother-to-child blocking in low viral load group was significantly lower than that in high viral load group ( χ2=5.87, P=0.015), and it was significantly lower in treated group than that in untreated group ( χ2=29.195, P=0.001). The percentages of HBsAb level <10 mIU/ml, 10- <100 mIU/ml and ≥100 mIU/ml in 1 297 children without HBV infection were 1.15%, 15.65% and 83.19%, respectively. Univariate and multivariate logistic regression analysis showed that maternal total bilirubin level and hypothyroidism during pregnancy were correlated with HBsAb level at 1 year of age ( χ2=29.003, P <0.05). Conclusions:Antiviral drugs taken during pregnancy by pregnant women with high HBV viral load can significantly reduce mother-to-child transmission of HBV. Follow-up on the response of children born to HBV-infected mothers to hepatitis B vaccine should be enhanced after birth.

Hepatitis B virus (HBV) is one of the infectious viruses that seriously endanger human health. There are more than 257 million people infected with chronic HBV in the world, and about and about 887 thousand people die of cirrhosis, liver failure and hepatocellular carcinoma caused by chronic HBV infection every year. It has caused serious harm to society and individuals. In China, the main route of transmission of chronic hepatitis B is mother-to-child transmission (MTCT). Therefore, blocking MTCT of HBV is an important measure to prevent the occurrence and development of hepatitis B. This article is to review the related research on blocking HBV MTCT at home and abroad, hoping to provide help for clinical practice.

Hepatitis B virus (HBV) can not cause hepatocellular disease directly after infecting the host. Instead, it causes cytopathic disease through the interaction with the host immune system, thereby advances the disease. Age, sex, ethnicity, etc. of HBV infected persons are associated with different clinical outcomes after HBV infects the host. However, different outcomes can even occur when the hosts have the same race, sex, and age are infected with the same HBV subtype in the same way. It suggests that the host immune genetic factors play a decisive role. The single nucleotide polymorphism (SNP) of innate immunity, adaptive immunity and related genetic factors in cytokine pathways is closely related to different clinical prognosis. This article mainly reviews the latest research progress in immune genetic factors association with chronic HBV infection, and summarizes the potential role and clinical significance of the host immune genetic determinants of chronic HBV infection, hoping to provide help for clinical practice.

ObjectiveTo evaluate the clinical effectiveness of doctor-nursing integration mode on the prevention of venous thromboembolism(VTE) in geriatric patients with hip fracture. MethodsFrom January to June of 2018, a total of 120 elderly patients with hip fracture admitted to the orthopaedic department of Ningbo First Hospital were selected in this study. According to the time of admission, they were divided into the control group (n=58)and the observation group (n=62). The conventional nursing mode was adopted in the control group and doctor-nursing integration nursing mode was adopted in the observation group. The two groups were compared in terms of the scores of VTE related knowledge, swelling degree of the affected limbs, the plasma D-Dimer level and the incidence of DVT on the lower limbs as well as the pulmonary thrombus. ResultsOne week after surgery, the patients in the observation group achieved higher score in the VTE related knowledge than the control group with statistical significance (P＜ 0.05); one day and one week after surgery, the observation group was lower than the control group in the swelling degree of the 10cm above the hip of the affected limb; one week and two weeks after surgery, the observation group was lower than the control group in the plasma D-Dimer level, both with statistical significance (P＜0.05); the control group's incidence of perioperative DVT on the lower limbs was 11.4% , lower than the 25.8% in the observation group with statistical significance (P＜ 0.05). ConclusionsDoctor-nursing integration nursing model can help to improve the compliance with the thrombosis prevention of the elderly patients with hip fracture, relieve the symptoms related to perioperative VTE and reduce the incidence of DVT on the lower limbs.