Objective To explore the reaction time and attention bias characteristics of patients with first-episode depressive disorder.MethodsTotally 32 patients with first-episode depressive disorder (MD group) and 24 non-depression control participants(NC group) matched with MD group on age,gender and education level were enrolled in the study.The clinical symptoms were assessed by Beck Depression Inventory(BDI).All participants completed a dot-probe task to assess attentional preference for facial stimuli with varying valence (happy,sad and neutral facial expressions).ResultsThe reaction times(RTs) of MD group was longer than that of NC group in the dot-probe task ((468.6±87.7)ms,(451.7±82.5)ms,P<0.01).The four-way ANOVA revealed a significant main effect of prime duration,indicating overall shorter RTs on primes with longer duration ((476.9±88.4)ms vs (456.2±82.7)ms vs (447.7±83.9)ms,P<0.01).Compared with NC group,the scores of attention bias for sad faces were decreased in MD group ((7.43±26.4)ms vs (-4.97±19.5)ms,P<0.05).With the longer duration of presentation,the score of attention maintenance of emotional facies for MD group were increased (happy faces: (-11.0±4.8)ms,(2.2±6.9)ms,(6.1±8.5)ms;angry faces:(-1.6±7.5)ms,(6.5±8.6)ms,(14.9±6.7)ms).The adherence score of attention to happy faces were decreased ((1.8±5.6)ms,(-8.2±6.7)ms,(-8.7±7.1)ms),while the score of adherence score towards sad faces were increased ((-7.6±7.2)ms,(-2.6±8.5)ms,(1.5±6.2)ms) with increasing prime duration.ConclusionPatients with first-episode depressive disorder have slower response to emotional faces and associated with attentional bias for sad faces.With the increasing prime duration,it is more and more obvious to attentional bias in the two aspects of allocation and adherence.

Aim To establish a mouse breast cancer model stab-ly expressing HER2. Methods 4T1-Luc mouse breast cancer cell line was transfected with the full-length human HER2 gene and selected with G418. The HER2 expression in 4T1-Luc stable cells was detected by fluorescence-activated cell sorting ( FACS) and Western blot. 4T1-Luc/HER2 cells were implanted into the mammary fat pads of BALB/c or nude mice. After tumor stabili-zation, mice were randomly assigned into 4 groups for treatment with PBS control, chA21, Trastuzumab, or chA21 plus Trastu-zumab. Tumor volumes were measured and tumor growth inhibi-tion ratios were calculated twice a week. At the end of experi-ment, tumor metastasis in mice was detected by bioluminescence imaging technology. Results Several 4T1-Luc/HER2 stable cell clones were obtained after G418 selection. FACS and West-ern blot analysis showed that all clones expressed HER2 protein at high levels. These 4T1-Luc/HER2 clones showed good tumor-igenicity in mice with steady tumor growth after one week of cell implantation. After 2-3 weeks, metastatic tumor cells were seen in the lung, cheek and groin areas. In BALB/c mice, the tumor growth inhibition ratio was 43. 3% in chA21 plus Trastuzumab group (P<0. 05 vs PBS control), which was higher than chA21 group (11. 1%) or Trastuzumab group (23%). In addition, the luminescence number and density of tumor metastases in lungs were significantly reduced in the antibody combination group. Conclusions The mouse model of spontaneously metastasizing breast cancer with HER2 overexpression is successfully estab-lished. The preliminary study suggests that anti-HER2 antibody combination of chA21 and Trastuzumab has excellent inhibitory effects on tumor growth and metastasis.

Objective To knockout the MATP gene of mouse melanoma cell line B16F10 using CRISPR/Cas9 system,and to lay foundation for the functional study of MATP gene.Methods Specific primers of MATP were designed according to the report in http://crispr.mit.edu/ website.The primers were linked to pCAS9/gRNA1 vector.Then the positive vector was transfected into mouse melanoma B16F10 cells,and monoclonal cell lines were obtained by the infinite dilution method.After the genomes of different monoclonal cell lines were extracted and sequenced,the cell lines with MATP gene cleavage were screened,and the expression of MATP in these cell lines was verified by Western-blot analysis.Results Three MATP gene knockout cell lines were successfully obtained.The western-blot results showed that the cell lines did not express MATP protein.Conclusions The knockout of MATP gene in B16F10 cell line can be successfully achieved using the pCAS9/gRNA1 vector.

The construction of close-knit county medical communities is an initiative to promote primary care in counties and practice Healthy China.The internal and external environments with uncertainty,variability,and complexity threaten the survival space and integration mode of the medical establishment in the close-knit county medical communities,and the resource-based capabilities and static core capabilities can no longer fully meet the survival and development needs of the medical and health institutions in the compact county medical communities in the dynamic environment.It proposes a conceptual framework for the dynamic capabilities of medical and health institutions in compact county medical and health communities,clarifies that the dynamic capabilities of medical and health institutions are divided into four core dimensions,namely,perception capability,learning capability,coordination capability,and integration capability,and analyses the mechanism of the evolution of the dynamic capabilities of medical and health institutions in compact county medical and health communities in conjunction with the SECI model,and finally proposes to do a good job in the top-level design of the medical community policy,to form the external"push"of the dynamic capabilities,and to use the SECI model as a basis for the development of the dynamic capabilities.thrust,enhance the resource coordination and integration capacity with medical community information technology as a push,and enhance the enhancement strategy to improve the dynamic capacity of medical and health institutions with the focus on the construction of health human resources.

The construction of close-knit county medical communities is an initiative to promote primary care in counties and practice Healthy China.The internal and external environments with uncertainty,variability,and complexity threaten the survival space and integration mode of the medical establishment in the close-knit county medical communities,and the resource-based capabilities and static core capabilities can no longer fully meet the survival and development needs of the medical and health institutions in the compact county medical communities in the dynamic environment.It proposes a conceptual framework for the dynamic capabilities of medical and health institutions in compact county medical and health communities,clarifies that the dynamic capabilities of medical and health institutions are divided into four core dimensions,namely,perception capability,learning capability,coordination capability,and integration capability,and analyses the mechanism of the evolution of the dynamic capabilities of medical and health institutions in compact county medical and health communities in conjunction with the SECI model,and finally proposes to do a good job in the top-level design of the medical community policy,to form the external"push"of the dynamic capabilities,and to use the SECI model as a basis for the development of the dynamic capabilities.thrust,enhance the resource coordination and integration capacity with medical community information technology as a push,and enhance the enhancement strategy to improve the dynamic capacity of medical and health institutions with the focus on the construction of health human resources.

The construction of close-knit county medical communities is an initiative to promote primary care in counties and practice Healthy China.The internal and external environments with uncertainty,variability,and complexity threaten the survival space and integration mode of the medical establishment in the close-knit county medical communities,and the resource-based capabilities and static core capabilities can no longer fully meet the survival and development needs of the medical and health institutions in the compact county medical communities in the dynamic environment.It proposes a conceptual framework for the dynamic capabilities of medical and health institutions in compact county medical and health communities,clarifies that the dynamic capabilities of medical and health institutions are divided into four core dimensions,namely,perception capability,learning capability,coordination capability,and integration capability,and analyses the mechanism of the evolution of the dynamic capabilities of medical and health institutions in compact county medical and health communities in conjunction with the SECI model,and finally proposes to do a good job in the top-level design of the medical community policy,to form the external"push"of the dynamic capabilities,and to use the SECI model as a basis for the development of the dynamic capabilities.thrust,enhance the resource coordination and integration capacity with medical community information technology as a push,and enhance the enhancement strategy to improve the dynamic capacity of medical and health institutions with the focus on the construction of health human resources.

The construction of close-knit county medical communities is an initiative to promote primary care in counties and practice Healthy China.The internal and external environments with uncertainty,variability,and complexity threaten the survival space and integration mode of the medical establishment in the close-knit county medical communities,and the resource-based capabilities and static core capabilities can no longer fully meet the survival and development needs of the medical and health institutions in the compact county medical communities in the dynamic environment.It proposes a conceptual framework for the dynamic capabilities of medical and health institutions in compact county medical and health communities,clarifies that the dynamic capabilities of medical and health institutions are divided into four core dimensions,namely,perception capability,learning capability,coordination capability,and integration capability,and analyses the mechanism of the evolution of the dynamic capabilities of medical and health institutions in compact county medical and health communities in conjunction with the SECI model,and finally proposes to do a good job in the top-level design of the medical community policy,to form the external"push"of the dynamic capabilities,and to use the SECI model as a basis for the development of the dynamic capabilities.thrust,enhance the resource coordination and integration capacity with medical community information technology as a push,and enhance the enhancement strategy to improve the dynamic capacity of medical and health institutions with the focus on the construction of health human resources.

The construction of close-knit county medical communities is an initiative to promote primary care in counties and practice Healthy China.The internal and external environments with uncertainty,variability,and complexity threaten the survival space and integration mode of the medical establishment in the close-knit county medical communities,and the resource-based capabilities and static core capabilities can no longer fully meet the survival and development needs of the medical and health institutions in the compact county medical communities in the dynamic environment.It proposes a conceptual framework for the dynamic capabilities of medical and health institutions in compact county medical and health communities,clarifies that the dynamic capabilities of medical and health institutions are divided into four core dimensions,namely,perception capability,learning capability,coordination capability,and integration capability,and analyses the mechanism of the evolution of the dynamic capabilities of medical and health institutions in compact county medical and health communities in conjunction with the SECI model,and finally proposes to do a good job in the top-level design of the medical community policy,to form the external"push"of the dynamic capabilities,and to use the SECI model as a basis for the development of the dynamic capabilities.thrust,enhance the resource coordination and integration capacity with medical community information technology as a push,and enhance the enhancement strategy to improve the dynamic capacity of medical and health institutions with the focus on the construction of health human resources.

The construction of close-knit county medical communities is an initiative to promote primary care in counties and practice Healthy China.The internal and external environments with uncertainty,variability,and complexity threaten the survival space and integration mode of the medical establishment in the close-knit county medical communities,and the resource-based capabilities and static core capabilities can no longer fully meet the survival and development needs of the medical and health institutions in the compact county medical communities in the dynamic environment.It proposes a conceptual framework for the dynamic capabilities of medical and health institutions in compact county medical and health communities,clarifies that the dynamic capabilities of medical and health institutions are divided into four core dimensions,namely,perception capability,learning capability,coordination capability,and integration capability,and analyses the mechanism of the evolution of the dynamic capabilities of medical and health institutions in compact county medical and health communities in conjunction with the SECI model,and finally proposes to do a good job in the top-level design of the medical community policy,to form the external"push"of the dynamic capabilities,and to use the SECI model as a basis for the development of the dynamic capabilities.thrust,enhance the resource coordination and integration capacity with medical community information technology as a push,and enhance the enhancement strategy to improve the dynamic capacity of medical and health institutions with the focus on the construction of health human resources.

The construction of close-knit county medical communities is an initiative to promote primary care in counties and practice Healthy China.The internal and external environments with uncertainty,variability,and complexity threaten the survival space and integration mode of the medical establishment in the close-knit county medical communities,and the resource-based capabilities and static core capabilities can no longer fully meet the survival and development needs of the medical and health institutions in the compact county medical communities in the dynamic environment.It proposes a conceptual framework for the dynamic capabilities of medical and health institutions in compact county medical and health communities,clarifies that the dynamic capabilities of medical and health institutions are divided into four core dimensions,namely,perception capability,learning capability,coordination capability,and integration capability,and analyses the mechanism of the evolution of the dynamic capabilities of medical and health institutions in compact county medical and health communities in conjunction with the SECI model,and finally proposes to do a good job in the top-level design of the medical community policy,to form the external"push"of the dynamic capabilities,and to use the SECI model as a basis for the development of the dynamic capabilities.thrust,enhance the resource coordination and integration capacity with medical community information technology as a push,and enhance the enhancement strategy to improve the dynamic capacity of medical and health institutions with the focus on the construction of health human resources.