Objective To study arsenic trioxide induces apoptosis of human cholangiocarcinoma cell line QBC939. Methods MTT was applied to detect the inhibitory effect of arsenic trioxide on cholangiocarcinoma cell line QBC939. Morphological changes were observed by light microscopy and fluorescence microscopy. Rhodamine123 dyeing and analysis of DNA content and cell cycle were examined by flow cytometry. Results Cell growth was significantly inhibited by different concentration(1-16?mol/L) of arsenic trioxide. The inhibition ratio was dependent on arsenic concentration and time. The typical morphological character of ~apoptosis was observed, apoptotic peak of hypodiploid was detected and fluorescence intensity of ~Rhodamine 123 decreased when the cells were cultured with 4?mol/L As_2O_3. Conclusions As_2O_3 can induce ~apoptosis of cell line QBC939. The mechanism may be related to the depolarization of mitochondrial ~membrane potential.

Objective To investigate the therapeutic effect of kansui root on patients with severe acute pancreatitis(SAP). Methods Clinical data of 54 cases of severe acute pancreatitis treated with kansui root(kansui root group) were analyzed and compared with 54 cases of severe acute pancreatitis treated without kansui root (control group).Results The releivng time of abdominal pain was significantly shorter than that in control group( P

Objective To investigate the timing of operation and the selection of surgical procedure for acute biliary pancreatitis.Methods The clinical data of 247 cases of acute biliary pancreatitis were retrospectively analysed.Results Among 10 non-operation patients,4 died;12 patients underwent emergency operation,5 had postoperative complications and 2 patients died after operation.169 patients underwent delayed operation after effective conservative therapy and 1 patient had postoperative complication;56 patients underwent(selective) operation without any postoperative complication or death.Conclusions Early emergency operation should be performed for relief of biliary obstruction in acute biliary obstructive pancreatitis.The patients with acute non-obstructive biliary pancreatitis should undergo conservative therapy at first and then,after(pancreatitis) has been controlled,delayed operation or selective operation to treat the biliary pathologic condition should be performed.