This paper reported nine cases of pancreatic lithiasis. Pancreatolithotomy and pancreaticojejunostomy was performed in 4 cases; Pancreatoduodenectomy in 1 case; Laparotomy and biopsy in 1 case complicated with pancreatic carcinoma and hepatic metastasis; And nonoperative treatment in 3 cases. Our data showed that pancreatic fluid stasis caused by stenosis of pancreatic duct and/or the distal segment of bile duct maybe one of the important factors of pancreatic stone formation. The diagnosis and principles of surgical treatment of pancreatic lithiasis were discussed.

Objective To detect the effect of exogenous IL-6 on the proliferation of cholangiocarcinoma cell line QBC939.Methods MTT was used to observe the proliferation of cell line QBC939 treated with exogenous IL-6.Following the annexin V/FITC and PI staining,FCM was used to detect the apoptosis of QBC939 treated with exogenous IL-6.Results The proliferation of cell line QBC939 was increased after treatment with IL-6 and was positively related with the concentration of IL-6(P

Objective To study the relationship between the microvesel density(MVD) ,vascular endothelial growth factor(VEGF) , matrix metalloproteinases 2 ( MMP2 ) with the metastasis, prognosis of cholangiocarcinoma. Methods MVD,expression of VEGF and MMP2 were investigated by means of immunohistochemistry in 45 cholangiocarcinoma tissues and 8 normal bile duct tissues. Results (1)MVD and positive expression rates of VEGF , MMP2 in cholangiocarcinoma tissues were significantly higher than those in normal bile duct tissues ( P

Objecctive To study the expression and significance of CD44V6 and E-Cd in gallbladder carcinoma. Methods The expression of CD44V6 and E-Cd was detected by immunohistochemical technique in 48 cases of gallbladder carcinoma, 29 cases of gallbladder adenomal polypi and 25 cases of chronic calculous cholecytitis. The association of CD44V6 and E-Cd expression with the clinical and pathologic parameters was analyzed. Results E-Cd expressed in 21 of 48 cases of gallbladder carcinoma(43 75%), 21 of 29 cases of gallbladder adenomal polypi(72 42%) and 24 of 25 cases of chronic calculous cholecytitis(96 00%), while CD44V6 expressed in 31 of 48 cases of gallbladder carcinoma(64 58%), 9 of 29 cases of gallbladder adenomal polypi(31 03%) and 1 of 25 cases of chronic calculous cholecytitis(4 00%). The expression rates of CD44V6 and E-Cd in the three kinds of tissues were significantly different(P

Objective To analyze the outcome of patients with acute obstruction of left colorectal cancer treated by modified antegrade colonic lavage with primary tumor resection and anastomosis.Methods From April 2002 to April 2007,112 patients with acute obstruction of left colorectal cancer underwent surgery.During the operation the left colon was exteriorized and placed into a sterilized plastic bag to protect the surgical field from contamination,then a catheter was inserted via the appendix,and after antegrade colonic lavage,primary resection and anastomosis was performed.Results Tumor resection and primary anastomosis was successfully done in the 112 cases.Postoperatively,1 case had anastomotic leak which healed after reoperation with proximal colostomy,and one patient died.Conclusions Modified antegrade colonic lavage is a simple procedure,the bowel can be rapidly decompressed with essentially no contamination,and has a high level of bowel cleansing.It is possible to safely perform primary resection and anastomosis for left colon cancer after the modified antegrade lavage.

Objective To investigate the influence of bile duct ligation ( BDL ) on macrophage ( M ) activity and bacterial translocation in mice . Methods After 7 days of BDL , the levels of serum total bilirubin(TB) , albumin(Alb) , glutamic - oxaloacetic transaminase(AST) , creatinine(Cr) , alkaline phosphatase(ALP) and nitrogen oxide(NO) producted by peritoneal macrophages were measured . The mesenteric lymph nodes were cultured for bacteria . The liver and kidney tissues were histopathologically examined. Results The levels of serum iTB,AST,AKP in BDL group were significantly higher than those in control group(P

Objective To investigate the expressions of phosphatase and tensin homolog deleted on chromosome 10(PTEN) and P53 protein in cholangiocarcinoma and evaluate their possible relationship and clinical significance. Methods The expressions of PTEN and P53 in 36 cases of cholangiocarcinoma, eight cases of normal biliary duct and 30 cases of para-neoplastic tissue were detected by S-P immunohistochemistry method. Results The positive rate of PTEN protein expression was 63.89%(23/36) in cholangiocarcinoma, which was significantly lower than that in normal biliary duct (100%,8/8) and that in para-neoplastic tissue(100%,30/30), and which had no relationship with tumor locus, but was significantly correlated with the degree of differentiation and metastasis. The positive rate of P53 protein expression was 44.44%(16/36) in cholangiocarcinoma, which was significantly higher than that in normal biliary duct( 0%, 0/8) and that in para-neoplastic tissue (0%, 0/30), and it had no relationship with tumor locus and metastasis,but was significantly correlated with the degree of differentiation. The PTEN protein expression had correlation with the expression of P53. Conclusion The expression of PTEN protein was significantly lower in cholangiocarcinoma and significantly correlated with the degree of differentiation and metastasis other than the locus of tumor. The expression of P53 protein was associated with the degree of differentiation, but not with the tumor locus and metastasis. Moreover, The PTEN protein expression had correlation with the P53 expression.

Objective To investigate the correlation of the expressions of PTTG and VEGF proteins in extrahepatic cholangiocarcinoma,and study its role in the development of extrahepatic cholangiocarcinoma.Methods Expression of PTTG and VEGF proteins was detected by SABC immunohistochemical technique in 36 cases of extrahepatic cholangiocarcinoma,30 cases of adjacent histologically noncancerous bile duct tissues and 12 cases of benign bile duct lesions.Results The positive rates of PTTG and VEGF proteins were(72.2)%(26/36) and 83.3%(30/36) respectively,in extrahepatic cholangiocarcinoma;and 63.3%(19/30) and 76.7%(23/30) in adjacent histologically noncancerous bile duct tissues.The expression of PTTG protein was significantly positively correlated with that of VEGF protein(P

Objective To discuss the diagnosis and treatment of insulinoma.Methods The clinical data of 30 patients with insulinoma were reviewed.Results All patients had Whipple′s triad.Accurate preoperative localization rate of B ultrasonography, CT and MRI was 34.8 %(8/23),58.3 %(7/12) and 71.4 %(5/7),respectively. Localization rate of intraoperative ultrasonography (IOUS) was 87.5 %(7/8). The tumors were single in 27 cases,and multiple in 3 cases. In the location of single tumor,8 of them were in the head,7 in the body, and 12 in the tail; while for multiple tumors, 2 tumors were both located in the body in 1 patient,and 2 tumors were separately located in the body and tail respectively in 2 patients. Local enucleation was performed in 21 cases, distal pancreatectomy in 6 cases,distal pancreatectomy plus splenectomy in 2 cases,and duodenopancreatectomy in 1 case. The tumor was benign in 29 cases, and malignant in 1 case.Pancreatic fistula developed after operation in 4 cases, and in all cases, it healed after drainage. All patients had no symptoms of hypoglycemia after operation. At follow-up visit in 27 cases, 1 case of benign tumor recurred 4 years after operation, and was cured by resection of the pancreas body with tumor; the malignant tumor case, recurred and died of metastasis of abdominal cavity 3 years after operation.Conclusions Whipple′s triad, and the ratio of immunoreactive insulin to blood glucose (IRI/G)are the bases for qualitative diagnosis of insulinoma. Meticulously palpating the gland combined with IOUS during operation is the most effective method for accurate tumor localization. Enucleation is the main mode of surgical treatment of insulinoma.

Objective To investigate the possible mechanism of pancreaticobiliary maljunction(PBM) on development of congenital choledochal cyst(CCC).Methods Tweenty-one patients with choledochal cysts initially diagnosed by ultrasonography,then underwent ERCP.The concentrations of amylase(AMS) and C-reactive protein(CRP) in bile removed through selective biliary catheterization was analyzed.Results Each of 21 cases of PBM(including 13 cases of C-P type and 8 cases of P-C type) had significantly higher concentrations of AMS and CRP compared with control group(P