OBJECTIVE: The aim of this study is to investigate the expression of hypoxia inducible factor-1alpha (HIF-1alpha), vascular endothelial growth factor (VEGF) in laryngeal carcinoma and its relations to clinical and pathophysiological characteristics, and determine the effect of HIF-1alpha, VEGF in the processing of angiogenesis. To offer the theoretical basis for the pathogenesis of laryngeal carcinoma and the new ideas for early diagnosis of laryngeal carcinoma. METHOD: The major was divided into two groups:60 specimens of laryngeal carcinoma and 20 specimens of normal tissues beside the carcinoma. The expression of HIF-1alpha, VEGF was detected in 60 specimens of laryngeal carcinoma tissues and 20 specimens of normal tissues beside the carcinoma as controls by immunohistochemical staining technique. Microvessel was stained by antifactor CD105 antibody to analyse microvessel density. RESULT: Positive rate of HIF-1alpha and VEGF protein expression was 71.67% (43/60) and 65.00% (39/60) respectively in laryngeal carcinoma tissues, which was significantly higher than that in normal laryngeal tissues (10.00% and 20.00%, respectively). HIF-1alpha expression was closely related to P-TNM stage, the grade of cell differentiation and lymph node status; VEGF expression was closely related to P-TNM stage and lymph node status. The coincidence rate of the expression of HIF-1alpha and VEGF was 56.67% (34/60), showing a close relation between them. MVD was much higher in tumor with HIF-1alpha(+)/VEGF(+) than that in tumor with HIF-1alpha(--)/VEGF(--) (P < 0.01). CONCLUSION HIF-1alpha protein and VEGF was over-expressed in laryngeal carcinoma. The positive expression of HIF-1alpha, VEGF and MVD in laryngeal carcinoma have a synergistic effect on the neovascularization of the tumor.
Female ; Humans ; Hypoxia-Inducible Factor 1, alpha Subunit ; metabolism ; Laryngeal Neoplasms ; blood supply ; metabolism ; Larynx ; metabolism ; Male ; Microvessels ; Neovascularization, Pathologic ; etiology ; Vascular Endothelial Growth Factor A ; metabolism

Colorectal cancer (CRC) is one of the most common digestive system neoplasms in China. Recently, the morbidity and mortality rates of CRC continue to increase with the improvement of people's living standard and the change of the dietary structure. It was accepted that the etiology of colorectal cancer is a multi-factor and multi-step process. However, the mechanism of CRC is still unclear. MicroRNAs (miRNAs) are a class of -21 nucleotide non-coding RNAs and function as a negative regulator of gene expression after transcription, participating in tumor angiogenesis, tumor cell proliferation and invasion. MicroRNA-21 (miR-21), as an important oncogenic RNA, plays a vital role in the development and progression of CRC. This review summarizes the advances in research on miR-21 in colorectal cancer.

Objective To study the audiometry and otoacoustic emissions in patients with posterior circulation ischemia(PCI) .Methods Forty patients treated by neurologists were selected as the experimental group who re_ceived pure tone audiometry ,tympanometry ,otoacoustic emissions (OAE) and distortion products otoacoustic emis_sions (DPOAE) examinations .Thirty healthy objects were chosen as the control group .The data from the PCI groups and the control group were compared using the SPSS 14 .0 software .ResuIts The difference in pure tone thresholds across 125 to 8 000 Hz between the PCI group and the control group was not statistically significant (P>0 .05) .In the PCI patients ,the prevalence of TEOAE was only 47 .5% ,significantly different from that of in the control group .The prevalence of DPOAE at 0 .5~8 kHz were between 57 .9% ~77 .6% in PCI patients ,and the amplitudes of DPOAE were reduced significantly (P<0 .05) .ConcIusion Cochlear damages can occur to patients with PCI ,especially in the high frequency range .These results suggest that OAE can be used as an important diag_nostic test for patients with PCI ,and might be helpful for the location diagnosis of PCI .

Objective] To investigate Bushenhuoxue method to improve the effect and mechanism of ovarian reserve, providing a theoretical basis for clinical treatment. [Methods] The intervention method to retrieve recent Bushenhuoxue premature ovarian failure and decreased ovarian reserve related literature, from theoretical research, clinical research and experimental study three aspects discussed Bushenhuoxue method to improve the effect and mechanism of ovarian reserve. [Results] Invigorating kidney on improving the exact effect of ovarian reserve. [Conclusion] In recent years Bushenhuoxue method research progress in improving ovarian function, mechanism of action has a more in-depth elucidation, but further in-depth exploration and research are stil needed.

Objective To investigate the incidence of adverse drug reaction (ADR)in patients in a hospital,and evaluate ADR-related factors,so as to provide references for the prevention of ADR and reducing of risk factors in drug use. Methods 223 clinically reported ADR cases were collected from January 1,2011 to December 31,2011, patients'age,types and administration routes of drugs relating to ADR,and ADR-involved organs and systems were analyzed.Results Of 223 patients with ADR,211(94.62% )received intravenous drip,3 received intravenous injec-tion,2 received intramuscular inj ection,and 7 received oral administration. ADR mainly occurred within 1-30 mi-nutes after taking the medicines,39(17.49% )occurred within 1-4 minutes,45(20.18% )within 5-9 minutes,80 (35.87% )within 15-29 minutes. Of organ or system involved by ADR,skin and its appendage damages were com-mon (93 cases),followed by gastrointestinal tract (62 cases)and systemic damage (45 cases);single organ and sys-temic damage were most common (184 cases,82.51% ). 123 cases (55.16% )of ADR were caused by antimicrobial use,60 cases (26.91% )were induced by Chinese medicine injection;Of top 10 ADR-inducing medicines,7 were an-timicrobial agents and 3 were traditional Chinese medicine injections,levofloxacin lactate and sodium chloride injec-tion ranked first(23 cases,10.31% ).Conclusion The occurrence of ADR is related to multiple factors,reporting and surveillance of ADR should be strengthened,the use of antimicrobial agents and traditional Chinese medicine should be rational,thereby reduce the occurrence of ADR.

Objective To investigate the effect of establishing external device quality management system in the operating room of primary hospital.Methods 100 pieces external orthopaedic surgical instrument cleaned and packaged by company personnel in 2012 were randomly selected as control group,and 100 packages of orthopaedic surgical instrument cleaned by the professional of our hospital were randomly selected and set as the observation group.The cleaning quality of the two groups were compared.Results The numbers of no blood on the surface,sur-face of the device without rust,instrument storage box no stains,cloth bag no stains in the control group were 92,91, 86,90,respectively,which in the observation group were 99,98,97,98.The cleaning quality improved obviously in the observation group,and there were statistically significant differences between the two groups (χ2 =4.19,471,7.78, 5.67,all P <0.05).Conclusion To establishment of surgical patients external device quality management system in the operating room of primary hospital,standardized management of external devices,can ensure that the management and implementation of quality control of external devices in all aspects and to ensure clinical safety.

OBJECTIVE: To study the hearing characteristics in patient with posterior circulation transient ischemic attack (PCTIA). METHOD: Sixty-two patients diagnosed by neurologist were selected as experimental group for testing of pure tone audiometry, tympanometry, otoacoustic emissions (OAE) and auditory brainstem response (ABR). Thirty healthy subjects were chosen as control group. The data were compared between the PCTIA groups and the control group using the SPSS 14.0 software. RESULT: (1) The difference in pure tone threshold across 125 to 8000 Hz between the PCTIA group and the control group was not statistically significant (P>0.05); (2) In PCTIA patients, the prevalence of TEOAE was only 42.7%, significantly different from the control group; (3) The patients had longer ABR latency and wave interval than the normal controls (P<0.01). CONCLUSION Brain-stem and cochlear damage can occur in patients with PCTIA. These results suggest that OAE and ABR be used as important diagnostic test for patients with PCTIA, and might be helpful in localizing abnormality in PCTIA.
Adult ; Aged ; Case-Control Studies ; Female ; Hearing ; physiology ; Humans ; Ischemic Attack, Transient ; physiopathology ; Male ; Middle Aged

OBJECTIVE: Applying Chinese version of categories of auditory performance (CAP) to assess cochlear implant (CI) recipients of prelingual deaf and test the reliability of Chinese version of CAP. METHOD: Fifty CI recipients of prelingual deaf were interviewed by a parent and a speech therapist using Chinese version of CAP. Kappa coefficient analysis was applied to test the reliability of Chinese version of CAP. RESULT: Thirty-five of 50 cases (70%) were found completely coincident, 12 cases (24%) were one category different, 3 cases (6%) were 2 categories different. Kappa coefficient was 0.624. CONCLUSION Chinese version of CAP was found reliable and useful to evaluate auditory performance of prelingual deaf pediatric after cochlear implantation.
Asian Continental Ancestry Group ; Child ; Cochlear Implantation ; Cochlear Implants ; Deafness ; therapy ; Hearing Tests ; Humans ; Reproducibility of Results ; Speech Perception ; Treatment Outcome

Objective Study the relationship between type A influenza virus genetic variation with survival selective pressure, help for the finding of possible vaccine conserved antigen target. Methods Select seven strains of same HA (Hemagglutinin) serotype, regional and isolation time closely related type A influenza virus with full HA gene coding sequence; use Blast2 program to calculate the parameter of nucleotide conservative, amino acid conservative, mutation ratio of codon 3rd (over) non 3rd locus, survival selective pressure indicator of these virus strains; analysis the parameters relationship with survival selective pressure indicator. Results Nucleotide conservative of HA gene is significantly lower than that of other genes; amino acid conservative of HA gene is similar with NS gene, all lower than that of other genes; genetic variation of HA gene is under survival selective pressure; selective pressure toward NS, PB1 gene is relatively lower than that toward NA, NP gene; survival selective pressure indicator is strongly correlated with mutation frequency upon codon 3rd locus. Conclusions Genetic variation of influenza virus is determined both by mechanism of relax replication model and survival selective pressure; genetic conservative of each gene is different, NA, NP gene could be selected as possible vaccine target for their relative high conservative, HA gene possesses medium genetic conservative with prospective of finding more conserved epitope region within its full sequence, NS, PB1 are not recommended as vaccine candidate for their relative low genetic conservative.

AIM: To study the effects of alltride on experimental gastric ulcer and promoting bowl movement. METHODS: Applying experimental gastric ulcer models induced by alcohol and hydrochloric acid, ligating pylorus, indomethacin, acetic acid, calculated the index of gastric ulcer. Applying dry stool constipations in mice the first defecation time, moist dejection amounts were observed. In small intestinal propelling test in normal mice, small intestinal contents (charcoal Powder) propelling ratio was observed. RESULTS: Alltride reduced the index of ulcers in above experimental gastric ulcer model rats (P