OBJECTIVE: To optimize the preparation technology for the lovastatin capsules.METHODS: Orthogonal test was used to optimize the conditions for the technology of preparation meanwhile a dissolution test was conducted.RESULTS: According to the results of the single factor analysis and orthogonal test,the optimal condition for the lovastatin capsules were as follows: agglomerant: 10%PVP;disintegrator: CMS-Na was used by combining exterior and interior addition(2∶1);stuffing capsule by granules;wetting agent of twice granulating: 75% alcohol.The 30 min dissolution rate of the capsules prepared in the optimized technology reached above 92%.CONCLUSION: This preparation is stable in technology,reproducible and with high dissolution rate.

Objective To explore the influences of metoprolol in the treatment of diastolic blood pressure on blood glucose.Methods 120 patients with diastolic blood pressure were selected and divided into the research group and the control group,each group 60 cases.The research group was given metoprolol,and the control group was given pelodipine.The treatment time was 12 months.In treatment process,bload glucose and pressure was regularly detected and compared between the two groups.Results After treatment,the diastolic blood pressure of the two groups was lower than that before treatmen(all P ＜ 0.05),but the difference of the diastolic blood pressure between the two groups had no statistical significance before and after treatment(all P ＞ 0.05).The fasting blood glucose(GLU),P2hPG(P2hPG) of the two groups in 1,3,6 months had no obvious difference with those before treatment,but after 12 months the treatment,P2hPG,GLU of the research group was(6.23±0.89) mmol/L,(7.46±0.71) mmoL/L,respectively,and was higher than those before treatment[(4.97±0.52) mmol/L,(5.21±0.69) mmol/L]and those of the control group at the same period[(5.13±0.48)mmol/L,(5.16±0.67) mmol/L(all P＜0.05).Conclusion Metoprolol in the treatment of diastolic blood pressure had good effect.Long term use of drugs(1 year or more) could increase blood glucose levels,but had no other clinical damage,and the safety was good.

Objective To profile the types of bacteria in bile culture and study their antibiotic sensitivity pattern in patients with varying de-grees of acute cholangitis and to provide data guidance for the prevention and treatment of bacterial infection.Methods The clinical data of 230 patients with acute cholangitis who were admitted to our hospital from April 2010 to April 2014 were retrospectively analyzed.Classifica-tion of pathogenic bacteria and the antibiotic sensitivity test in bile cultures were performed using VITEK 2 Compact Biomerieux microbiologi-cal system.Results Within the 230 patients,172 tested positive for biliary bacteria,and the positive rate was 74.78%(172/230).There were 237 strains of pathogenic bacteria,including 135 strains of Gram-positive bacteria (56.96%),comprised mainly of Enterococcus and Staphylococcus aureus,96 strains of Gram-negative bacteria (40.5 1%),comprised mainly of Pseudomonas aeruginosa,Escherichia coli, and Klebsiella pneumonia,and 6 strains of fungi (2.53%),comprised mainly of yeast.Significant difference in the positive rate of bacteria was detected between patients with mild and severe cholangitis (χ2 =4.58,P =0.028).Gram-negative bacteria were the predominant i-solates in patients with mild and moderate acute cholangitis,while multiple bacterial infection was more common in patients with severe cholangitis.Biliary bacteria had a higher rate of susceptibility to vancomycin,imipenem,and amikacin compared with other antibiotics. Conclusion Gram-positive bacteria are the predominant pathogens in acute cholangitis.Early detection,immediate intervention,and ac-curate drug susceptibility test should be implemented at regular intervals to direct effective clinical therapy.

The hospital the authom work with, the first one to undergo the second-cycle nation-wide hospital evaluation, was assessed by a third party and the hospital evaluation experts. The two cycles of hospital evaluation were entirely different in historical background, priorities and procedures. The evaluation standards for the second cycle, which were on a par with internafional ones, set the right track of development for medical institutions, emphasizing quality, security, service and performance and highlighting the delivery of quality patient care. The gains from the second-cycle hospital evaluation can be summarized as follows: more highly emphasized continuous improvement of service quality, enhanced awareness of people-oriented management, reinforced medical quality and medical security control, heightened hospital infection control, greater capability of responding to emergencies, and strengthened for-malation of hospital rules and regulations. In the end five suggestions are put forward with regard to the second-cycle hospital evaluation.

Objective:To study the chemical constituents in the seed of Cassia leschenaultiana.Methods: The compounds were separated and purified by column chromatography , thin-layer chromatography and recrystallization .The structures were identified by the physicoche mical identification and spectral analysis .Results:Seven compounds were isolated from the seeds of Cassia leschenaultiana and identified as 1-desmethylchryso-obtusin (Ⅰ) , aurantio-obtusin (Ⅱ) , ale-emodin (Ⅲ) , obtusin (Ⅳ) , chryso-obtusin (Ⅴ) , ob-tusifoline(Ⅵ)and aurantio-obtusin(Ⅶ).Conclusion:All of the compounds are isolated from the seeds of Cassia leschenaultiana for the first time.

Objective To establish a method for determining paraquat in the serum by ultraviolet spectrophotometry and present a quantitative index for paraquat poisoning salvage. Methods The sample was deproteinizated by 20% trichloracetic acid(TAC). 50 ?l microcell was used, the detection wavelength was 257 nm. Results The linearity was within 0.05～50 ?g/ml, r=0.999 9. The average recovery rates were 90.0%-102.4% and RSD=3.9%(n=4), the lowest detection limit was 0.01 ?g/ml. Conclusion The method is simple, rapid, precise and suitable for the determination of paraquat in the serum.

Objective:To establish the HPLC fingerprint of Cassia leschenaultiana from different regions. Methods: The column was SinoChrom ODS-BP (250 mm × 4. 6 mm, 5 μm). The mobile phase consisted of acetonitrile-0. 1% phosphoric acid with gradient elution. The flow rate was 1. 0 ml·min-1 , the detection wavelength was 285 nm, the column temperature was 25℃, and the injection volume was 10 μl. Results: The fingerprint consisted of 13 common peaks. The range of similarity for ten batches of Cassia le-schenaultiana was 0. 839-0. 998. And the reference fingerprint of Cassia leschenaultiana was established by HPLC. Conclusion: The fingerprint method is simple and reproducible, which can provide basis for the quality control and the medicinal resources exploration.

Objective: We investigated experimentally the changes of intestinal immunity following shock and resuscitation after trauma. Method:The experimental model was made in rats, which underwent laparotomy, then bleeding and reinfusing through the right fetmoral artery. Result: The concentration of IgA following shock and resuscitation were significantly higher than that before shock.. The concentration of IgA 24 h following resuscitation was the lowest, and was significantly lower than that at the end of shock.. The endotoxin in portal vein following shock and resuscitation were higher than that before shock.. The endotoxin level 24 h following resuscitation was the highest, and markedly higher than that at the end of shock. Conclusion: The traumatic shock and resuscitation are capable of causing intestinal immunosuppression and endotoxemia.

Objective To explore the expression of singal transducers and activators of transcription(STAT 3) during focal cerebral ischemic reperfusive injury in rats and the relationship between ischemic neuronal damage and it.Methods Using immunohistochemical method of avidinbiotin peroxidase complex (ABC) we observed the distribution of positive cells in STAT 3 protein immunoreaction after focal cerebral ischemic reperfusive injury in rats.Results STAT 3 immunoreactive positive cells were not found in the cortex and striatum of normal and sham operative rat brains and nonischemic hemisphere brain after cerebral ischemia,small amount of STAT 3 immunity positive cells were induced in the embolism la teral infarction area 12 h after reperfusive injury,and peaked after 24 h,especially in ischemic lateral striatum and around cortex,small number of nerve cells in around infarction still showed positive expression after one week.The difference had remarkable significance( P

Objective To study the therapeutic effect of pulse-activating injection on acute poisoning by pa-raquat(PQ). Methods 50 Sprague-Dawley (SD) rats were randomly divided into five experimental groups: blank group, negative control group, positive control group, low-dose pulse-activating injection group (LDG), and high-dose pulse-activating injection group(HDG) (n = 10 for each group). Blank group were injected with normal suline,30 ml/kg,and other groups were established as acute paraquat poisoning models. Macroscopic and histopathological ex-aminations were performed and biological indexes were measured for the lung specimens. The indexes included lung wet weight/dry weight,the rats of neutrophils and protein content in the pulmonary alveolar lavage fluid. In the mean time, the level of malondialdehyde (MDA), superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) both in plasma and bronchoalveolar lavage flui(BALF) were detected. Results Compared with that in negative control group,lung congestion and lung edema of LDG group were mitigated; and the MDA level decreased from (5.04± 0.50) nmol/ml,(1. 19±0.18) nmol/ml to (4.04±0.21) nmol/ml,(0.79±0.04) nmol/ml both in plasma and BALF;the SOD activities were increased from (123.30±20. 39) U/ml, (26.43±2.22) U/ml to (277.09± 11.66) U/ml,(37.10± 2.49) U/ml as well; the GSH-Px activities were increased from (1796.63 ±81. 12) U/ml, (598.24 ± 62.50) U/ml to (2151.54 ± 148.32) U/ml, ( 1788.44 ± 175.11 ) U/ml as well ( P < 0. 01 ). Conclusions Administration of pulse-activating injection could improve the lipid peroxidation damage caused by a-cute poisoning of PQ.