Objective To evaluate the pain control, general condition and well-being of the patients with advanced cancer treated with transdermal fentanyl as well as the effects of transdermal fentanyl on heart rate variability (HRV) and endocrine function. Methods This open prospective study included 40 patients with advanced cancer. The direct conversion ratio from oral morphine to transdermal fentanyl system (TFS) was 100:1 daily. In patients who had never received any opioid before the initial dose of TFS was 25?g/h. The transdermal system was changed every 72h and the dosage was adjusted to the need of the patients according to the VAS scores and the requirement of morphine. 8 hospitalized patients were monitored by 24h Holter for changes in HRV. Blood samples were taken before transdermal fentanyl patch when pain was severe and 24h after TFS for determination of ACTH,?-endorphin, growth hormone(GH) and insulin levels.Results Pain relief during treatment with transdermal fentanyl was significant accoring to VAS scores. Well-being scores showed that patients' quality of life including appetite, sleep, mental state and daily activities was significantly improved after two weeks treatment. The ratio of LF vs HF, which is one of HRV frequency indices, was obviously reduced at 24h after transdermal fentanyl patch as compared with that before TFS (P

With the profound study of PDT mechanism and the new type of photosensitizers,the application of PDT to hematopoietic diseases has become a new domain.The relapsing/refractory aggressive lymphoma is one of the tough problems which are still unsolved in hematopoietic domain. Now,the experimental and clinical research on application of PDT in lymphomas has become the hot topic for the domestic and world scholars.

Objective To observe the effect of acupuncture on apoptosis and Caspase-9 expression in hippocampus of cerebral ischemia (CI) rats. Methods Thirty male SD rats were randomized into control, CI and CI + acupuncture group, with 10 cases in each group. CI model was established by occlusion of the unilateral middle cerebral artery. "Baihui" (GV20) and "Shuigou" (GV26) were acupunctured once daily for 7 days. The apoptosis and expression of protein Caspase-9 in hippocampus was displayed by HE staining and immunohistochemical method. Results In comparison with the normal control group, Caspase-9 immune reaction (IR) positive cells in the hippocampus of CI group increased significantly (P

Objective To prepare the chitosan-Mg membranes and to explore the biocompatibility of membranes with PC12 cells and its usage as biomaterials in tissue engineering.Methods The appearance of the chitosan-Mg membranes (CM) was observed under scanning electromicroscope (SEM) and the element of the membranes was analyzed by x-ray energy spectromete. PC12 cells were co-cultured with CM in vitro,the morphological changes of PC12 cells on membranes were observed under scanning electron microscope (SEM) and light microscope (LM),the cell vitality was detected by MTT assay. Results The surface of chitosan membranes (CS) was smoother than that of CM;but the CM groups were full of pore observed under SEM;The content of Mg element was related with the dosage of MgSO4 added into chitosan solution. Morphology observation showed that PC12 cells grew well on CM as compared with CS group. The cells were rich of microvilli and long processes on 7th day,and synapses-like structure was formed in many PC12 cells. In addition,the cell viability of experiment group was higher than that of control group(P

Gypsum Fibrosum is one of mineral medicines, which is widely used in clinics for a long time. It is mainly used for treating epidemic febrile diseases and excess-heat syndrome. The studies on the heat-clearing effects and mechanisms of Gypsum Fibro-sum in recent years were summarized in the paper,which can provide scientific reference for its further research, application and the heat-clearing mechanism exploration.

Objective To further explore the effects of acupuncture and Fuyuan Mixture(FM) on learning and memory. Methods One hundred and twenty_nine NIH male mice were allocated to six groups:Group A (normal control group),Group B(model group),Group C(treated with acupuncture),Group D(treated with FM),Group E(FM and acupuncture),and Group F(treated with nimodipine). Model mice with acquired memory disorder were induced by scopolamine.Baihui,Dazhui,Shenting and Zusanli were chosen as the acupoints.FM is composed of Cornu Cervi Pantotrichum,Radix Ginseng,Rhizoma Polygonati,Fructus Lycii,Fructus Alpiniae Oxyphyllae,Radix Salviae Miltiorrhizae,etc..The methods of platform jumping and finding food in complex maze were used to observe their learning and memory. Results Compared with Group B,incubation period was prolonged,electric shock time shortened and errors reduced at 20 min and 24h after modeling in Group C and Group E,and time for finding food in complex maze was shortened in Group C and Group D(P

Objective To observe the effect of acupuncture on protein Calbindin-D28k expression in hippocampus of rats with cerebral ischemia,and to investigate its influence on rats neurological behavior.Methods Male SD rats were randomized into blank control group,model group and acupuncture group,10 rats in each group.The rat model of focal cerebral ischemia was established by chemical-stimulation-induced occlusion of the unilateral middle cerebral artery.Acupuncture group received acupuncture of acupoints of Baihui and Shuigou,one time per day for 7 days.The changes of protein Calbindin-D28k expression in hippocampus of rats were observed before and after treatment,and the score of rats neurological behavior was observed after the modeling for 1~2 hour(s)and after treatment.Results Calbindin-D28k expression was decreased in the hippocampus of model group(P

Objective To clone and sequence the dystonin variant X1 gene of Cricetulusbarabensis and the albino mutant Cricetulusbarabensis so as to find out the difference of encoding arear of the muscular ribosome between Cricetulusbarabensis and the albino mutant Cricetulusbarabensis.Methods According to the same type of abnormal muscle tone protein of the mice and rats, we designed 6 pairs of primers, and got their cDNA genes from skins of the Cricetulusbarabensis and the albino mutant Cricetulusbarabensis by RT-PCR amplification, then cloned and sequenced. Results Sequence alignment showed 17 variances in coding areas, and 24 in amino acid, but no in key nucleic acid and protein.Conclusion The variances in coding areas will not lead to the albino, and its mechanism requires further investigation.

Objective To detect the relationship of blood pressure variability (BPV) and angiotensinogen(AGT) T235M polymorphism with Binswanger's disease (BD).Methods Totally 122 cases with BD and 108 cases with essential hypertension had been enrolled.Ambulatory blood pressure monitoring was used to get the data of BPV and mean blood pressure (MBP).PCR-RFLP was applied to detect genotype of AGT T235M.Results Distribution of MM+MT genotype and frequency of M allelic were higher in BD group (51.6％,29.9％) than in hypertension group(27.8％,16.2％)(x2 ＝13.543,11.995,P＜0.01).The variability of night time blood pressure (11.8±2.8,9.1±2.5) and 24 hours diastolic blood pressure(11.6±6.0) in BD group were increased compared with those in hypertension groups(10.9±2.4,8.2±3.2,10.1±4.6)(t＝2.59,2.64,2.09,all P＜0.05).Prevalence of anti-dipper was higher in BD group (32.8％) than in EH group (16.7％)(x2 ＝7.894,P＜0.01).Among BD patients,anti-dipper who carried MM or MT genotypes (44.4 ％) was more than that who carried TT genotype (20.3％) (x2 ＝ 8.760,P＜ 0.01).Conclusions Fluctuations of nighttime blood pressure and diastolic pressure are higher in patients with BD,and the relative frequency of MM or MT genotype is higher.Moreover,among patients with BD who carried MM or MT genotypes are apt to have anti-dipper.MM or MT genotype is related to BD by means of affecting blood pressure circadian rhythms probably.

Objective To summarize the perioperative nursing of undergoing radical cystectomy with ileal neobladder for the treatment of bladder cancer? Method The nursing measures included preoperative psychological care,bowel and body preparation as well as postoperative nursing of drainage tube,ileal bladder irrigation,bladder functional exercise and basic nursing care? Results No patients suffered from postoperative complications such as infections,urinary fistula and anastomotic leakage? Twenty patients recovered autonomous urination within 3-6 months after the operation? One patient got minor urinal incontinence? Conclusions Cystectomy with orthotopic ileal neobladder trauma contributes to more complications due to large surgical trauma? Therefore,good perioperative care is important for the successful operations?