Treatment of CIN2～3 includes cervical ablation,cervical local excision and hysterectomy,while cervical local excision is the standard procedure for CIN2～3.The recurrence rate was 0.7%～21% after surgery treatment of CIN2～3;The high risk factors of recurring are positive margin,serious lesion and age over 40.The follow-up items for postoperation involve cytology,colposcopy,ECC and HPV testing;If there were 2 LSIL or 1 HSIL for cytology,the patients should be given colposcopy examination and ECC.Retreatment must be clinically individual one.It is the same significance for follow-up and treatment of CIN2～3.

Objective:To study the chemical constituents in the seed of Cassia leschenaultiana.Methods: The compounds were separated and purified by column chromatography , thin-layer chromatography and recrystallization .The structures were identified by the physicoche mical identification and spectral analysis .Results:Seven compounds were isolated from the seeds of Cassia leschenaultiana and identified as 1-desmethylchryso-obtusin (Ⅰ) , aurantio-obtusin (Ⅱ) , ale-emodin (Ⅲ) , obtusin (Ⅳ) , chryso-obtusin (Ⅴ) , ob-tusifoline(Ⅵ)and aurantio-obtusin(Ⅶ).Conclusion:All of the compounds are isolated from the seeds of Cassia leschenaultiana for the first time.

Constant infusion, exponentially declining infusion and program infusion of theophlline were administered to rabbits byadopting the rabbit population pharmacokinetic parameters in this laboratory, and the plasma concentrations of the drug was measured by ul- traviolet spectrophotometry. The results showed that the plasma drug concentrations following exponentially declining infusion and program infusion attained the desired steady-state level at only 30 min, though the T1/2?= 6. 08h, The median absolute value of performance error in pro-gram infusion and exponentially declining infusion was 7. 8% and 14% respectively.

Objective To observe the influence of individual behavior intervention on emotion and quality of life of elderly females with stress urinary incontinence and provide guidance for health intervention.Methods 124 elderly females with stress urinary incontinence were selected.Individual behavior intervention was used,and after 1,3,6 months,the effect was evaluated by anxiety,depression,ICIQ -FLUTS,UDI -7,and SCL -90.Results After the individual behavior intervention,anxiety and depression scores became lower[anxiety scores:before intervention (59.16 ±6.12)points,1month (52.68 ±5.62)points,3months (48.28 ±5.32)points,6months (39.86 ± 4.27)points,there were statistically significant differences between the two groups(F =298.69,P =0.000).Depres-sion scores:before intervention (59.16 ±6.12)points,1 month (52.68 ±5.62)points,3 months (48.28 ± 5.32)points,6 months (39.86 ±4.27)points,there were statistically significant differences between the two groups (F =252.10,P =0.000)].ICIQ -FLUTS and UDI -7 scores became less[ICIQ -FLUTS scores:before interven-tion (31.79 ±3.74)points,1 month (29.62 ±3.18)points,3 months (27.54 ±2.67)points,6 months (23.32 ± 2.16)points,there were statistically significant differences between the two groups(F =161.15,P =0.000).UDI -7 scores:before intervention (19.29 ±1.76)points,1 month (16.09 ±1.48)points,3 months (14.32 ±1.37)points, 6 months (11.57 ±1.26)points,there were statistically significant differences between the two groups(F =531.23, P =0.000)].There were significant differences in somatization,obsession,sensitivity of interpersonal relationship, depression,anxiety,hostility,fear,crankiness and psychotic diseases(somatization scores:F =18.933,P =0.000;obsession:F =5.153,P =0.000;sensitivity of interpersonal relationship:F =3.314,P =0.020;depression:F =9.306,P =0.000;anxiety:F =4.902,P =0.002;hostility:F =6.852,P =0.000;fear:F =7.782,P =0.000;cranki-ness:F =11.917,P =0.000;psychotic diseases:F =8.560,P =0.000).Conclusion After the individual behavior intervention,anxiety,depression,ICIQ -FLUTS,UDI -7,and scl -90 scores become lower.Individual behavior inter-vention has a positive effect on emotional states and quality of life,which is worthy of clinical promotion.

OBJECTIVE To strengthen the management of medical refuse in order to control the onset of hospital(infection.) METHODS It has been done including setting up special tissue,formulating supervising system,strengthening training,carrying out supervising measures and strengthening examination and inspection.(RESULTS) It could prevent hospital infection effectively to regulate the whole course of the medical refuses(collection,) storage,dealing with and registration.CONCLUSIONS It is very important to strengthen the management of medical refuse in order to prevent hospital infection.

Objective:To establish the HPLC fingerprint of Cassia leschenaultiana from different regions. Methods: The column was SinoChrom ODS-BP (250 mm × 4. 6 mm, 5 μm). The mobile phase consisted of acetonitrile-0. 1% phosphoric acid with gradient elution. The flow rate was 1. 0 ml·min-1 , the detection wavelength was 285 nm, the column temperature was 25℃, and the injection volume was 10 μl. Results: The fingerprint consisted of 13 common peaks. The range of similarity for ten batches of Cassia le-schenaultiana was 0. 839-0. 998. And the reference fingerprint of Cassia leschenaultiana was established by HPLC. Conclusion: The fingerprint method is simple and reproducible, which can provide basis for the quality control and the medicinal resources exploration.

With the profound study of PDT mechanism and the new type of photosensitizers,the application of PDT to hematopoietic diseases has become a new domain.The relapsing/refractory aggressive lymphoma is one of the tough problems which are still unsolved in hematopoietic domain. Now,the experimental and clinical research on application of PDT in lymphomas has become the hot topic for the domestic and world scholars.

Objective To evaluate the effects of consolidation chemotherapy on postponing the relapse in patients with advanced epithelial ovarian carcinoma. Methods 44 patients with advanced epithelial ovarian carcinoma treated in our hospital from March 2000 to April 2004 were enrolled. Clinical complete remission was achieved after standard treatments in all these patients, and they were randomly assigned into consolidation group and control group. Relapse rate and Disease-free Survival were analyzed. Results 22 patients were assigned to consolidation group, and 22 patients as control group. The latest follow up examination was done in July 2005. Relapse rate was 45.5% in consolidation group, and 59.1% in control group (P=0.365). Mean relapse time in consolidation group was 25.3?9.3 months, and was 16.9?6.7 months in control group (P=0.019). Mean Disease-free Survival was 31.9?14.8 months in consolidation group, and was 22.7?12.9 months, in control group (P=0.033). Kaplan-Meier Survival Analysis showed no significant difference P=0.22. Conclusion Consolidation chemotherapy may postpone tumor relapse and prolong Disease-free survival in patients with clinical complete remission advanced epithelial ovarian carcinoma. While it hasn′t been proved but the results in present study did not prove that consolidation chemotherapy could lower relapse rate or elevate survival rate. Further study is needed to achieve better results.

Objective To investigate the effects of siRNA silencing enhancer of zeste homolo 2 (EZH2) on invasion and migration of cervical cancer cells and the molecular mechanisms.Methods The small interfering RNAs (siRNA) targeting EZH2 were transiently transfected into C33A cell line by lipofectamin2000.The effects of EZH2 on cell invasion and migration were detected by wound-healing assay, Transwell assay and soft agar colony assay.The expression of MMP2 was detected by Western blot.Results Downregulation of EZH2 expression by siRNA in C33A cell line significantly inhibited cell invasion and migration in vitro.Meanwhile, siRNA-mediated depletion of EZH2 reduced the expression of MMP2.Conclusion Knocking down EZH2 expression by siRNA could surpress invasion and migration of human cervical cancer cells, which might be related to downregulating MMP2 expression.

Objective To investigate the efficacy of Lu’s warm needling moxibustion with silver needles plus external application of antiphlogistine and specific electromagnetic spectrum in compositely treating knee osteoarthritis. Method Sixty patients with knee osteoarthritis were randomly allocated to treatment and control groups, 30 cases each. The treatment group received warm needling moxibustion with silver needles plus external application of Ruyi antiphlogistine and specific electromagnetic spectrum and the control group, intraarticular injection of sodium hyaluronate plus oral administration of celecoxib. The clinical therapeutic effects were compared between the two groups. Result The total efficacy rate was 93.3% in the treatment group and 73.3% in the control group; there was a statistically significant difference between the two groups (P<0.05).The knee pain score (P<0.01,P<0.05), the daily physical function score (P<0.01,P<0.05) and the knee stiffness score (P<0.01,P<0.05) decreased in the two groups after treatment compared with before. After treatment, the knee pain score, the knee stiffness score and the daily physical function score decreased more in the treatment group than in the control group (P<0.05,P<0.01). Conclusion Lu’s silver needle acupuncture as main therapy can significantly improve the symptoms of knee osteoarthritis.