Forty-five male mice were used and divided into three groups: i.e. experimental gastric ulcer group, saline control group and normal control group. After experimental gastric ulcer was induced, the mice of three groups received intraperitoneal injection of colchicine and were sacrificed 3h later at 3, 6, 9 and 20 days, respectively. The antral mucosa was removed and processed by Sternberger's immunocytochemical PAP method to show G cells and counterstained with hematoxylin. In normal control group, the mitotic index of the antral mucosal epithelial and glandular cells was 5.93?1.23; the percentage of G cells was 2.76?0.45; the mitotic index I of G cells (the number of the mitotic G cells per 100 G cells) was 0.85?0.18; and the mitotic index II of G cells (the number of mitotic figures of the G cells per 100 antral epithelial, glandular and G cells) was 0.02?0.01. The mitotic index of the antral mucosal epithelial and glandular cells, the percentage and the mitotic index II of G cells on 6th, 9th and 20th days in experimental gastric ulcer group was raised and showed highly significant statistical difference from that of the control group, respectively. The mitotic index I of G cells was raised on 9th day in the experimental gastric ulcer group and significant difference between experimental gastric ulcer group and the control group was found. It also revealed a significant diference in the experimental gastric ulcer group as compared with saline control group on 20th day. The percentage of G cells on 6th day was most high, but the peak of mitotic number of G cells appeared on 9th day in the experimental gastric ulcer group. The distribution of G cells was found upward in the glands near the ulcer on 3rd and 6th day than in normal control. These findings suggest that the number, origin, distribution and shape of the G cells in the pyloric glands exhibited dynamic changes with the passage of time. The results suggested that the G cells might participate in the regulation of regeneration of antral mucosa during experimental gastric ulcer.

Objective Studies on the changes of gastrin and SS immunoreactive cells in pancreatic islets during experimental gastric ulcer. Methods The immunohistochemical ABC technique was used. Results The gastrin immunoreactive cells were located in most of the pancreatic islet. The mumber of G cells in experimental gastric ulcer group were higher than that of control group on the 4th and 10th day after operation.The D cells raised on the 10th day.Conclusion The present work provides the evidence that the G and D cells of pancreatic islets might be involed in the self-healing process of the experimental gastric ulcer by endocrine or paracrine regulation.

Objective To explore preoperative psychological experience in patients with the tibia and fibula fracture that treated with 3D printing individualized external fixation, and provide theoretical basis for the medical treatment and care. Methods From December 2013 to November 2015, sevenpatentstreated with 3D printing individualized external fixation in Xi′an Honghui Hospital, Xi′an Jiaotong University Health Science Center were selected as the interview object. This qualitative study was completed by phenomenon research methods, and combined with a variety of forms of interview.Colaizzi content analysis method was used to analyze the contents of collected data, and sort and refine the theme. Results Four effective subjects were extracted about preoperative psychological experience in patients with the tibia and fibula fracture treated with 3D printing individualized external fixation, in turn were uncertainty effect of surgery; high expectations, but the lack of knowledge about 3D printing, economic burden of disease; longing for family support system. Conclusions Medical staff should pay attention to preoperative psychological feelings in patients with the tibia and fibula fracture treated with 3D printing individualized external fixation, deal with a targeted psychological nursing and coping style, provide an effective nursing intervention and health education. further more optimize the 3D external fixation cost,and incorporate the 3D external fixation into the social medical insurance category.