Objective To determine the relationships between maternal serum copper,amniotic copper,lysyl oxidase (LOX) and collagen Ⅲ in pregnant women with premature rupture of membranes (PROM) and without PROM.Methods One hundred women with PROM were enrolled in this study,and divided into 37-42 weeks,34-36~ +6 weeks and 28-33~ +6 weeks according to gestational age. One hundred non-PROM pregnancies matching the same gestational ages were recruited as control group. Copper of maternal serum and amnion in two groups were compared by FAAS method. Amniotic LOX was analyzed by fluorometry. Amniotic collagen Ⅲ was detected by immunohistochemical method and computer image analysis system(absorbance,A). Linear correlation analysis was used to explore the relationships between maternal serum copper,amniotic copper,LOX and collagen Ⅲ. Results (1)For 37-42 weeks pregnant women,serum copper was correlated positively with amniotic copper in two groups, r= 0.82(P0.05),but amniotic LOX and collagen Ⅲ decreased significantly compared with controls, being [(0.53?0.10)?g/g vs (0.75?0.10)?g/g,P

Objective To study the practicability of the Chemically-Activated Luciferase eXpresson(CALUX)bioassay used as a method for determination of dioxin in environmental epidemiological surveys.Methods The toxicity equivalents of dioxin in the same samples of body fat,blood and mothers milk were determined by high resolution gas chromatography/mass spectrometry(HRGC/MS )and the CALUX bioassay respectively and the relativity of the results obtained by the two different methods was analyzed.Results The correlation coefficients of the results of dioxin toxicity equivalents in body fat and mothers milk determined by the two methods were0.8675and0.9581respectively,which had significant positive correlation(P