Objective:To establish a method for the determination of protocatechuic acid, protocatechuic, catechin, epicatechin and rutin in Begonia grandis Dry. Methods:The 5 active ingredients in the samples were extracted by 70% methanol and analyzed by ultra performance liquid chromatography (UPLC). Chromatographic separation was performed on a Waters ACQUITY HSS T3(2. 1 mm × 100 mm, 1. 8 μm) column with gradient elution, and the mobile phase was solvent A: acetonitrile and solvent B:0. 2% glacial acetic acid (0-12 min:A:5→25 and B:95→75;12-30 min:A:25→50 and B:75→45). The effluent was monitored by a VWD detector set at 280 nm. The flow rate was set at 0. 2ml/min and the injection volume was 1μl. The column temperature was maintained at 30℃. Results:The calibration curves were all linear (r≥0. 99) over the test ranges. The average recovery was 96. 92%-99. 75%(n=6). Conclusion:The established method can rapidly obtain an accurate and reproducible result, which can be used to control the quality of Begonia grandis Dry.

OBJECTIVE:To establish a method for simultaneous determination of vicexin 2,vicexin 1 and schaftoside in Shilin-tong tablet. METHODS:HPLC was performed on the column of Phenomenex C18 with mobile phase of methanol-0.1% phosphoric acid (gradient elution)at the flow rate of 0.8 ml/min,the detection wavelength was 334 nm,the column temperature was 25 ℃, and the injection volume was 10 μl. RESULTS:The linear range was 0.31-3.10 μg for vicexin 2 (r=0.9999),0.28-2.80 μg for vicexin 1(r=0.9999)and 0.39-3.90 μg for schaftoside(r=0.9999),respectively;RSDs of precision,stability and reproducibili-ty tests were lower than 3.0%;recoveries were 95.2%-100.4%(RSD=1.6%,n=9),96.3%-100.8%(RSD=1.4%,n=9) and 95.7%-100.9%(RSD=1.7%,n=9),respectively. CONCLUSIONS:The method is simple,sensitive ,accurate,reliable and repro-ducible,and can be used for the quality control of Shilintong tablet.

OBJECTIVE:To investigate the effects of amylum pregelatinisatum on the dissolution of calcium dobesilate capsule.METHODS:The amount of amylum pregelatinisatum was optimized by means of the determination of angle of repose and the in vitro dissolution,using the single factor design.The comparisons of effects of different manufactory of amylum pregelatinisatum on formulation and dissolution of capsule were done.RESULTS:Amylum pregelatinisatum could improve significantly the fluidity of powder of capsule.The dissolution of calcium dobesilate capsule were decreased with the increment of amylum pregelatinisatum.It demonstrated that the fluidity of powder and dissolution of calcium dobesilate capsule were the best when the ratio of amylum pregelatinisatum and microcrystalline cellulose was 1∶3 and the amount of sodium carboxymethyl starch was 4 %.The different resource of amylum pregelatinisatum had great effect on the dissoution of the formulation.CONCLUSION:Amylum pregelatinisatum is a favourable filler adjuvant of capsule,but its quantity and the quality should make an optimization.

Objective To analyze imaging feature of multislice spiral CT(MSCT)in diagnosis of carotid artery diseases and to evaluate it’s dianostic significance.Methods MSCTA in 80 cases were performed in our study,of them,15 patients were undergone DSA simultaneous.The imaging quality of MSCTA was evaluated and the accuracy of MSCTA in diagnosis of carotid artery diagnosis by comparing the results of CTA and DSA in the same one patient.The calcifications in vessel wall displayed by various CTA reconstruction technique were analysed.Results The results of DSA and MSCTA 60 branches of carotid artery were analysed comparatively.The specificity and sensitivity of MSCTA in diagnosis of carotid artery stenosis was 85.71% and 92.7% respectively,the negative value was 95%,Kappa value was 0.81.Furthermore,CTA could display the artery wall well,it was easy to acquire good imaging quality.Conclusion As one noinvasive method of examination,CTA will play a common role in diagnosis of carotid artery disease.

Objective:To detect the expression of microchromosome maintenance protein 4 (MCM4) in pancreatic ductal adenocarcinoma, and to explore the clinical prognosis of MCM4 and pancreatic ductal adenocarcinoma. To explore the possibility of MCM4 as a potential biomarker for pancreatic ductal adenocarcinoma.Methods:The mRNA level of MCM4 in pancreatic ductal adenocarcinoma and normal tissues adjacent to the cancer was analyzed by bioinformatics methods, and the relationship between its expression and the survival rate of patients with pancreatic ductal adenocarcinoma was analyzed. The clinicopathological data of 76 patients with pancreatic ductal adenocarcinoma who underwent surgical treatment were retrospectively analyzed. Immunohistochemical method was used to detect the expression level of MCM4 protein in pancreatic ductal adenocarcinoma tissue and normal tissues adjacent to the cancer, and to analyze its relationship with clinicopathological characteristics of patients with pancreatic ductal adenocarcinoma.Results:The results of bioinformatics analysis showed that MCM4 mRNA was significantly highly expressed in pancreatic ductal adenocarcinoma tissue, and was significantly correlated with the overall survival rate and disease-free survival rate of patients (all P<0.05). Immunohistochemical results showed that the expression of MCM4 in pancreatic ductal adenocarcinoma tissue was significantly higher than that in normal tissues adjacent to the cancer. The high expression of MCM4 in pancreatic ductal adenocarcinoma tissue was related to tumor size ( P=0.038), but not related to age, gender, and tumor grade (all P>0.05). Conclusions:MCM4 is highly expressed in pancreatic ductal adenocarcinoma tissues and indicates a poor prognosis, and its expression level is related to the tumor stage of pancreatic ductal adenocarcinoma. MCM4 may serve as a new potential biomarker for pancreatic ductal adenocarcinoma.

AlM: To evaluate the effectiveness of Spot Vision Screener on vision screening of children without cycloplegia.METHODS:A total of 87 children (174 eyes) aged from 2~9 years old were examined with Spot Vision Screener and optometrist before cycloplegia.RESULTS: Statistical analysis demonstrated that the cylinder diopter and axis, the equivalent spherical diopter in both eyes, and the spherical diaopter in left eye had no significant change ( P>0. 05 ). However, the spherical diaopter in right eye had statistical significance. CONCLUSlON: Spot Vision Screener is a suitable instrument in vision screening of children without cycloplegia.

Objecitive The research was in order to explore the roles of MMP-2,MMP-3 and MMP 9 which played in the gastric cancer cell metastasis,and make a further study of the clinical signifance.Methods Both SGC-7901 and GES-1 were cultured in vivo.The migra-tion were examined by Wound-healing and the Transwell assay.The expression of MMP-2,MMP-3 and MMP 9 in the medium were detected by ELISA.The positive rate of MMP-3 in the gastric cancer,gastritis and normal was used the IHC,and the serum of MMP-2,MMP-3 and MMP-9 were also examined by ELISA.Results Both Wound-healing and Transwell assay showed that the migration of SGC-7901 cells was more quicker than the GES-1.Comparing to GES-1,the MMP-2,MMP-3 and MMP-9 were higher in the SGC-7901.The immunohistochemical results showed that the positive rate of MMP-3 in the gastric cancer patients was 84.4%,it was higher than the latter.The serum levels of MMP-2 MMP-3 and MMP-9 were also significantly higher than others.Conclusion The migration of gastric cancer cells was depended on the MMP-2,MMP-3 and MMP-9.The 3 proteins can be used for the diagnosis of gastric cancer patients,and it also avail to make the judgment the prognosis of patients with gastric carcinoma.

Objective To observe the influence of recombinant human brain natriuretic peptide(rhBNP)on serum HMGB1 levels in canines'acute renal injury induced by endotoxin and explore its protective role of rhBNP in protecting canines'kidney against acute renal injury.Methods A total of 20 healthy dogs were randomly divided into four groups:blank group,sepsis shock group,low-dose intervention group and high dose intervention group, and there were 5 rats in each group.After establishing the model of canines'sepsis shock induced by endotoxin,15 canines (besides blank group)were randomly divided into 3 groups.As follows,5 μg/kg rhBNP was given to the low-dose intervention group,10μg/kg rhBNP was given to the high-dose intervention group.But nothing was given to control group.Systemic vascular resistance index(SVRI)at 0 h,2 h,4 h,8 h,12 h were observed by PICCO instrument.High mobility group box 1 protein (HMGB-1)and creatinine(CR)in blood samples at each time point were detected.After 12 hours,kidney samples were taken for histological examination.Results The results revealed that some renal tubulars epithelial cell were swelled,some epithelial cells were atrophy and interstitial cells swelled in control group under the light microscope.Kidney pathology score was 2-3.But these changes were improved in low-and high-dose intervention groups,and there were no significant difference in the latter two groups,kidney of both groups pathology score were 1-2.Compared with control group at the same point,CR of blood serum were significantly decreased in low-dose intervention group at 8 h,12 h(P<0.01), and high-dose intervention group significantly decreased at 4 h,8 h,12 h(P<0.01).Compared with low-dose intervention group at the same point,CR of blood serum in high-dose intervention group were significantly decreased at 4 h,8 h,12 h (P<0.05).Compared with control group at the same point, systemic vascular resistance index(SVRI)were significantly decreased in low-dose intervention group at 2 h (P<0.01 ),but significantly decreased in high-dose intervention group at 2 h and 4 h (P<0.01).Compared with low-dose intervention group at the same point,SVRI in high-dose intervention group were significantly decreased at 4 h (P<0.05 ).Compared with control group at the same point,the expressions of high mobility group box 1 protein (HMGB-1)in blood serum in low- and high-dose intervention groups were significantly decreased(P<0.01);Compared with low-dose intervention group at the same point,the expressions of HMGB-1 in blood serum in high-dose intervention group were significantly decreased (P<0.05).Conclusion rhBNP can effectively reduce canine kidney tissue injury mediated by endotoxin and improve kidney function,reduce SVRI,and its therapeutic effect of rhBNP was in a dose-response relationship.rhBNP can effectively reduce HMGB-1 levels in blood serum of sepsis shock canines,which may be associated with the decrease of late inflammatory factor HMGB1.

[Objective]To establish an HPLC method for the determination of magnolol and honokiol in Baolong Pill.[Methods]The samples were analyzed on an Agilent C18 column(4.6mm?250mm,5?m).The mobile phase was a mixture of MeOH-H2O (78∶22);the rate of flow was 1.0 m1/min;the detection wave-length was 294 nm.[Results]Calibration graph was linear in the range of 0.02~0.26?g,r= 0.999 9 for magnolol;0.012~0.156?g,r= 1 for honokiol.The recovery was 100.46%,RSD=1.00% for magnolol;100.76%,RSD=1.37% for honokiol.[Conclusion] The method is sensitive and its results are accurate with good reproducibility.It can be used for the quality control of Baolong Wan.

Objective To observe the effect of healthy side limb training on stroke patients with hemiplegia.Methods 160 stroke patients with hemiplegia were divided into the observation group and control group.All patients in both groups were given routine rehabilitation,but the patients in the observation group were given the healthy side limb training.All patients were evaluated with Brunnstrom grades,Bathel Index(BI),Hamilton Depression Scale(HAMD)and the function of healthy side hand before and 5 months after treatment.Results After treatment,the function of trouble side limb of patients in both groups increased significantly compared with those before treatment.The scores of Brunnstrom grades,BI,HAMD and the function of healthy side hand of patients in the observation group increased significantly compared with those in the control group(P<0.01).Conclusion The healthy side limb training may improve the function of trouble side limb,activities of daily living and depression of stroke patients with hemiplegia.