Objectives To detemline whether apoptotic cell death is involved in rat cardiac allograft rejection and investigate the relevance of apoptosis with acute rejection and its implication.Methods Groups of Wistar rats underwent heterotopic heart transplantation from allogeneic SD or syngeneic Wistar rats.The cardiac grafts were harvested at 1,3,5,or 7 days after transplantation and underwent the detection of apoptotic cell death using in situ terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick end labeling(TUNEL).Histopathological rejeclion grade and apoptotic index(AI)were analyzed.Results The incidence of apoptotic cells was increased steadily over time in allografts,in contrast to syngeneic grafts.The apoptotic cells in allografts were mainly cardiac myocytes and few infiltrating lymphocytes.The AI of rejection grade 1,2,3 and 4 was significantly higher than that of rejection grade 0(P<0.01).Conclusions TUNEL can display apoptosis of single cell in situ.Apoptosis is an important mechanism of tissue injury in acute cardiac allograft rejection in rats.Myocyte apoptosis can be used as a valuable index to estimate the injury of grafts and monitor acute rejection.

Female ; Genital Neoplasms, Female ; classification ; pathology ; Humans ; Ovarian Neoplasms ; pathology ; Papillomavirus Infections ; Precancerous Conditions ; pathology ; Uterine Cervical Neoplasms ; pathology ; virology ; World Health Organization

The effect of Fusarin C, a new mutagen extracted from Fusarium moniliforme,on murine peritoneal M? activation by measuring M?-mediated MTC ADCC and CS,Fusarin C could inhibit M? activation by both MAF and FDP. The inhibitory effect was dose-and time-dependent and the curves of dose-and time-response were similar in all three assays. There is, however, apparent difference in Fusarin C dose and time needed to induce significant in hibition among these assays. Significant inhibition occured at 0.4?g/ml and 0.5?g/ml for MTC and CS respectively, but 1.6?g/ml for ADCC. Similarly, the minimal period of time necessary to bring about significant inhibition was 2 hr for MTC, but 3 hr for ADCC. Finally, significance of the inhibitory effect of Fusarin C on M? anti-tumor activity in relation to carcinogenesis was discussed.

The inhibitory effect of Fusarin C,a new mutagen isolated from Fusarium moniliforme,on M? activation was investigated.The inhibitory effect of Fusarin C disappeared partially after 24 hr culture in the absence of the mycotoxin and completely after 72 hr.In addition,it could be overcome by high concentrations of M? activating factor or anti-serum to the target cells.

Objective To evaluate the effect of Toxoplasma gondii infection on immune function of the rat recipients and onset of Toxoplasmosis after heart transplantation and its correlation with the use of Cyclosporin A(CsA). Methods ELISA was used to detect recipient's specific circulating antigen (CAg) and antibodies (IgG, IgM) after the transplantation. T lymphocyte subsets in peripheral blood were examined by using immunofluorescence stain and flow cytometry (FCM) before and after heart allograft 5,10,15,20 days in rats. Results The use of CsA increased the risk of infection by T. gondii and accelerated the increase of CD8 + T lymphocyte after the transplantation. The incidence of donor acquired T.gondii infection was higher than that of reactivated silent infection in recipients before operation. The percentage of CD8 + T lymphocyte was evidently elevated due to the onset of toxoplasmosis and the ratio CD4 +/CD8 + was reduced or inverted in the meanwhile. Conclusion The immune suppression after use of CsA was the main reason leading to an activation of the silent infection of T.gondii . CD8 + was the main cytotoxic cell elevated during the infection.

Acute Kidney Injury ; Humans ; Terminology as Topic

Objective To prepare HCC-targeted lipid ultrasound microbubble containing 10-hydroxycamptothecin (10-HCPT), and to assess its targeting function in vitro. Methods After the biotinylated monoclonal antibody Hab18 was prepared, the biotinylated degree was determined. Microbubbles containing 10-HCPT were prepared by mechanical vibration. Then the biotinylated antibody was attached to the surface of the microbubbles by avidin-biotin interaction. The physical property, entrapment efficiency and the drug-loading amounts of 10-HCPT lipid microbubbles were determined. The combination of biotinylated Hab18 with microbubbles containing 10-HCPT was proved by immunofluorescent assay, and the targeting function of the targeted microbubbles containing 10-HCPT was observed with light microscope, served non-targeted microbubbles as control group. Results About 13 biotin molecules were coupled to each antibody in average. The disposition of the prepared microbubbles was steady and the mean diameter was 1.52 μm. The drug entrapment efficiency was 76.32% and the drug-loading amounts was 21.81%. Red fluorescence was observed at the edge of the microbubbles in immunofluorescent assay, and the conjugation of the targeted microbubbles containing 10-HCPT with 7721 cells was tight while control group was negative. Conclusion HCC-targeted lipid ultrasound microbubbles containing 10-HPTC can be prepared successfully, with higher entrapment efficiency and drug-loading amounts and strong targeting function in vitro.

Adolescent ; Adult ; Aged ; Electrodes ; Epistaxis ; surgery ; Female ; Hemostasis, Endoscopic ; methods ; Humans ; Male ; Middle Aged ; Young Adult

Adolescent ; Adult ; Dimethylformamide ; adverse effects ; Female ; Humans ; Kidney ; drug effects ; physiopathology ; Lipid Metabolism ; drug effects ; Liver ; drug effects ; physiopathology ; Male ; Middle Aged ; Occupational Exposure ; adverse effects ; Young Adult

Objective:To investigate the clinical characteristics and risk factors of alcoholic liver disease (ALD) combined with type 2 diabetes mellitus (T2DM).Methods:The clinical data of 70 patients with ALD combined with T2DM (ALD + T2DM group) who received treatment from March 2015 to March 2020 in the First Affiliated Hospital of Guangxi Medical University were retrospectively analyzed. The clinical data of 69 patients with ALD (ALD group) and 69 patients with T2DM (T2DM group) who concurrently received treatment were also analyzed. Sex, age, body mass index, drinking habits and other basic information in the three groups were collected. The risk factors of ALD combined with T2DM were evaluated by logistic regression analysis.Results:The daily alcohol intake and years of drinking in the ALD + T2DM group were (110.97 ± 79.78) g/d and (25.17 ± 10.05) years, respectively, which were significantly higher than (91.48 ± 64.26) g/d and (21.78 ± 8.91) years respectively in the ALD group ( t = 1.699, 2.102, both P < 0.05). The incidence of metabolic syndrome in the ALD + T2DM group was 34.3% (24/70), which was significantly higher than 15.9% (11/69) in the ALD group ( χ2 = 6.210, P < 0.05). The activity ratio of aspartate aminotransferase/alanine aminotransferase, total bilirubin level, gamma glutamyl transpeptidase activity in the ALD + T2DM group were 1.59 ± 0.93, (64.73 ± 39.90) μmol/L, (522.93 ± 353.66) U/L, respectively, which were significantly higher than (1.04 ± 0.53), (10.37 ± 4.51) μmol/L, (35.73 ± 23.99) U/L, respectively in the T2DM group ( t = 4.280, 3.780, 5.045, all P < 0.05). Triacylglycerol level in the ALD + T2DM group was significantly higher than that in the ALD group [(1.69 ± 1.04) mmol/L vs. (1.28 ± 0.87) mmol/L, t = 2.523, P < 0.05). Prothrombin time and lactate dehydrogenase activity in the ALD + T2DM group were (13.13 ± 2.79) s and (226.17 ± 79.93) U/L, respectively, which were significantly higher than (10.41 ± 0.84) s, (172.63 ± 39.34) U/L, respectively in the T2DM group ( t = 7.715, 4.969, both P < 0.05). Multivariate Logistic regression analysis showed that prothrombin time, triacylglycerol level, years of drinking, gamma glutamyl transpeptidase activity, and amount of drinking were the main risk factors for ALD combined with T2DM ( OR = 2.010, 3.270, 1.230, 1.060, 1.006, all P < 0.05). Conclusion:Patients with ALD combined with T2DM are prone to metabolic syndrome and lipid disorders, which may aggravate the disease. Prothrombin time, triacylglycerol level, years of drinking play an important role in the development of ALD combined with T2DM.