1.Effects of losartan and amlodipine on plasma leptin and adiponectin levels and insulin sensitivity in obese hypertensive patients
Chang-Mei LIU ; Hui-Pu XU ; Hui-Ying XU ; Chang-Shan LIU ;
Chinese Journal of Endocrinology and Metabolism 1985;0(02):-
Objective To observe the effects of losartan and amlodipine on blood pressure,plasma levels of leptin,adiponectin and norepinephrine (NE) and insulin sensitivity in obese hypertensive patients.Methods The levels of plasma leptin and adiponectin were determined by RIA and the plasma NE level was assayed by high- performance liquid chromatography.Insulin resistance index (HOMA-IR) was evaluated using the homeostasis model.Results Compared with basal levels,after 16-weeks' therapy,plasma leptin,HOMA-IR and body mass index were significantly decreased in losartan group [(35.6?18.5 vs 32.0?17.1)?g/L,P
2.A Modified Dye Test for Toxoplasma gondii Infection
Hui XU ; Anping NI ; Qingtao CUI ; Ying HAO
Chinese Journal of Parasitology and Parasitic Diseases 1997;0(06):-
A modified dye test with microplate was to be established to detect Toxoplasma antibodies with cell-cultured Toxoplasma gondii. Numbers of stained and unstained tachyzoites were estimated in every 100 tachyzoites in each well after dyeing with methylene blue. The dilution with 50% tachyzoites stained was used as final dilution. Better results of the microplate dye test has been received when the concentration of tachyzoites in suspension reaches 109/ml with 1% sodium citrate as accessory factor.
5.Mesenchymal stem cells repair cisplatin-induced acute kidney injury via regulating miR-92b
Ying ZHOU ; Huitao XU ; Wei LI ; Jin YANG ; Hui QIAN
Chinese Journal of Clinical Laboratory Science 2017;35(5):321-325
Objective To investigate the molecular mechanism of bone marrow mesenchymal stem cells (BM-MSCs) in repairing cisplatin-induced acute renal injury.Methods The rats were injected 6 mg/kg of cisplatin intraperitoneally,and bone marrow mesenchymal stem cells (BM-MSCs group) or PBS (PBS group) were injected respectively via tail vein after 24 hours.The rats without injecting cisplatin were selected as a normal control group.The repair effect of BM-MSCs on renal injury was observed by HE staining and immunohistochemistry.In addition,NRK-52E cells were cultured in vitro and treated with cisplatin for 6 hours.Then,NRK-52E cells were continued to culture for 48 hours or co-cultured with BM-MSCs for 48 hours,and NRK-52E cells untreated with cisplatin were used as a control.The expression levels of miR-92b and its target gene PTEN were detected by qRT-PCR,and the expression level of p-Akt by western blot.Results HE staining showed that the tubular protein casts in BM-MSCs group were significantly less than that in PBS group,and that the renal tubular structure was significantly improved in BM-MSCs group.Immunohistochemical staining indicated that the number of cells expressing proliferating cell nuclear antigen (PCNA) in BM-MSCs group (131.0 ± 14.4) was significantly higher than that in PBS group (42.2 ±6.1,t =11.28,P <0.01).qRT-PCR results showed that in the vivo experiment,compared with the expression level of miR-92b and PTEN in the normal control group (1.11 ± 0.78,1.01 ± 0.21),PBS group were (4.64 ± 1.06) and (0.61 ± 0.2),respectively (all P < 0.05);BM-MSCs group were (2.27 ± 0.81) and (1.1 ± 0.1),respectively (all P < 0.05).In vitro experiment,compared with the expression level of miR-92b and PTEN in the negative control group (1.12 ± 0.77,1.02 ± 0.13),cisplatin group were (7.64 ± 0.72) and (0.58 ± 0.2),respectively (all P < 0.05),cell group were (4.38 ± 0.50) and (1.15 ± 0.23),respectively (all P < 0.05).Western blot results showed that compared with the expression level of p-Akt in cisplatin group (0.96 ± 0.18),p-Akt expression in cell group was (2.11 ± 0.11,P < 0.01).Conclusion BM-MSCs may repair the cisplatin-induced acute renal injury via down-regulating the expression level of miR-92b.
6.Effects of Aplysin on ethanol-induced oxidative damage in rat primary hepatocytes
Ai SU ; Hongyan ZHU ; Hongwei XU ; Ying LIU ; Hui LIANG
Chinese Pharmacological Bulletin 2016;(2):251-257
Aim To investigate the protective effects of Aplysin on ethanol-induced oxidative damage in rat pri-mary hepatocytes. Methods Rat primary hepatocytes were obtained via the portal vein collagenaseⅣin situ perfusion technique followed by a Percoll density gradi-ent centrifuge. MTT test was used to determine the op-timum dose of Aplysin and ethanol, and detect the cell vitality in primary hepatocytes. Supernatants of primary hepatocytes were harvested to measure AST and LDH level, and the SOD, GSH-PX activities and MDA con-tent in primary hepatocytes were observed. Flow cy-tometry was used to detect the cell apoptosis rate. DNA damage in primary hepatocytes was detected by single-cell gel electrophoresis assay. The level of mitochon-drial membrane potential in primary hepatocytes was tested by fluorogenic probe JC-1 . The CYP2 E1 activity in primary hepatocytes was detected by colorimetry. The proteins of CYP2 E1 were detected by Western blot. Results 300 mmol·L-1 dose of ethanol and 30 mg·L-1 dose of Aplysin were the optimal dosages and were used in the subsequent experiments. Hepatocyte vitality was significantly increased in Aplysin group compared to that in ethanol group, and Aplysin inhibi-ted the release of AST and LDH(P<0. 05). For Apl-ysin treatment group, the activities of hepatocyte SOD and GSH were significantly increased, and MDA was markedly lowered as compared with those in ethanol group( P <0. 05 ) . Aplysin could alleviate hepatocyte apoptosis significantly, and hepatocyte DNA damage rates of Ⅱ ~Ⅲ level and Ⅳ level were significantly lowered in Aplysin treatment group as compared with those in ethanol group, and Aplysin had evident im-provement in alcohol induced mitochondria damage of hepatocyte. Primary hepatocyte activities and protein expression of CYP2 E1 were markedly lowered in Aply-sin treatment group as compared with those in ethanol group(P<0. 05). Conclusion Aplysin has protective effects on liver oxidative damage induced by alcohol of primary cultured rat hepatocytes by blocking CYP2 E1 activation, relieving oxidative stress, and sharpening the oxidation resistance ability.
7.Construction of base training content system of military NBC health support base
Ying ZHANG ; Song DU ; Hui PAN ; Erqing LEI ; Xiegu XU
Military Medical Sciences 2015;39(12):909-911
Objective To complete the construction of base training content systems of military NBC health support base in combination with practical experience.Methods The concept, construction idea and the multi-dimension element of the military NBC health support basewere analyzed by data studies and expert interview method.Results and Conclusion The construction idea of the training model and the roadmap of the training system are proposed.
8.Problems of WeChat public platform in academic libraries of traditional Chinese medicine and measures for their solution
Hui XU ; Ma SUO ; Yujia SUN ; Hongwei ZHANG ; Ying ZHOU
Chinese Journal of Medical Library and Information Science 2016;25(8):53-57
Descibed in this paper are the problems in WeChat public platform construction in academic libraries of traditional Chinese medicine, including low litilization, ordinary columns, indistinct charaterized service, non-spe-cific recommendation of resources , poor charaterized knowledge interaction , and poor popularization , with measures proposed for their solution hoping that they would play an active role in promoting WeChat public platform construc-tion.
9.Analysis of proteomic spectra in serum from patients with laryngeal carcinoma by SELDI-TOF-MS technology
Ying XU ; Shengduo QIAO ; Hui HUANGFU ; Binquan WANG
Journal of Clinical Otorhinolaryngology Head and Neck Surgery 2009;(24):1116-1119
Objective:To screen the tumor markers of laryngeal carcinoma and to investigate their expression in preoperative and postoperative serum.Method:The distinct protein in serum was detected in 32 cases of laryngeal carcinoma and 38 healthy people by IMAC-Cu proteinchip array and surface-enhanced laser desorption/ionization time-of-flight mass spectrometry(SELDI-TOF-MS).The distinct proteins in serum were detected in 32 cases of laryngeal carcinoma preoperation and within 10 days 15 cases of laryngeal carcinoma postoperation with the same methods.The discriminatory profiling between preoperative and postoperative patients was analyzed by Biomarker Wizard software and Biomarker Pattern software.Result:The results showed that fifteen differentially expressed proteins in serum were screened by analysis of protemic spectra of preoprative patients and normal subjects.Seventeen kinds of protein differentially expressed in serum were screened by analysis of protemic spectra of preoperative and postoperative patients.Six kinds of protein(2 958.52,3 796.89,5 148.86,6 115.57, 052.18,and7 770.76)were obtained for making up patterns that was able to class the preoperative-team and postoperativeteam.Corresponding correct ratio were 84.38%(27/32)and 73.33%(11/15).Conclusion:The preliminary results suggest that classification system will provide a highly accurate and innovative approach for the arly dioagnosis of laryngeal carcinoma and judgement of prognosis.SELDI-TOF-MS technology is a useful tool for a high throughput screening of large-sized serum samples tO discover potential biomarkers for laryngeal carcinoma.