BACKGROUND:More recently, stem cel therapy has become an issue of concern. Exogenous neural stem cel transplantation brings new hope for the treatment of nervous system injury by self-replication and differentiation to complement and replace damaged or dead nerve cels.
OBJECTIVE:To explore the therapeutic efficacy of neural stem cel transplantation on Alzheimer’s disease.
METHODS:Thirty APP/PS1 mice with Alzheimer’s disease were randomly assigned into model group, cel solution transplantation group or cel transplantation group (n=10 per group). Another 10 C57BL/6 mice were selected as controls. Embryos of C57BL/6 mice at 18 embryonic days were taken to make neural stem cel suspension folowed by transfection using lentiviral vectors carrying GFP gene at different multiplicities of infection (1, 5, 10, 15, 20). Afterwards, GFP-transfected neural stem cels were implanted into the hippocampus of Alzheimer’s disease mice in the cel transplantation group, while the same volume of complete medium was injected into the hippocampus of mice in the cel solution transplantation group. Morris water maze test was performed at 2 weeks after cel transplantation, and brain tissues of mice was taken and detected histologicaly at 4 weeks after cel transplantation.
RESULTS AND CONCLUSION: Compared with the control group, the escape latency was significantly higher, and the number of crossings over the target quadrant was lower in the other three groups (P < 0.05). Compared with the cel solution transplantation and model groups, in contrast, the escape latency was significantly lower, and the number of crossings over the target quadrant was significantly higher in the cel transplantation group (P < 0.05). Four weeks after transplantation, more intact neurons were found in the cel transplantation group as compared with the model group. These findings indicate that neural stem cel transplantation can improve behavior and morphology performance of mice with Alzheimer’s disease.

Neonatal hyperbilirubinemia is one of the major causes of neonatal hospitalization,which is common in the early stage of the newborn and is characterized by skin and sclera yellow staining.The levels of serum total bilirubin and unconjugated bilirubin increased greatly in children,which may damage the brain function,kidney function,hearing and heart function of the newborn,even cause death.Research has found that hyperbilirubinemia can damage the brain nerve,hearing,kidney and heart of the newborn.The damage degree of target organs is positively related to serum bilirubin level.This article reviews the adverse effects of hyperbilirubinemia on newborns.

Objectives To investigate the specific site for measuring inferior alveolar nerve canal (IANC) by cone beam computed tomography (CBCT),and to provide reference data of the course of the IANC of the crowd.Methods This study was a retrospective data analysis.One hundred and thirty CBCT cases were measured and compared after classification by gender and age (65 men and 65 women,65 middle age and 65 youth).Since the proximal center of the mental foramen area,the cross-section at which the mental hole just completely breaks the buccal cortical bone was defined as S1,and S2,S3,S4 were defined as cross-sections with 1-cm apart from each other since S1.L1,L2,L3,and L4 were measured as the distance from specific sites on the outer wall of the IANC to the alveolar crest,the outer wall of lingual lateral cortical bone,the mandibular minimum margin,and the outer wall of buccal cortical bone.The results were analyzed using independent samples t test,with P＜0.05 being considered statistically significant.Results On the right IANC S2L1,S3L1,S4L1,S2L2,S3L2,S4L2,S1L3,S3L4,the 8 groups of L values measured on these 8 sites,all showed differences between genders (P＜0.05).So did the 10 groups of L values measured on the left IANC S1L1,S2L1,S3L1,S4L1,S1L2,S3L2,S4L2,S2L3,S3L3,S4L4 (P＜0.05).The middle age group and the youth group were statistically different for the data measured on the right IANC S2L4 (P＜0.05).This was the same case for the data measured on the left IANC S1L3.When classified according to gender,L1 in the S1 to S4 sections firstly increased and then decreased,and eventually decreased.The change pattern of L3 was opposite to that of L1.L2 in the S1 to S4 sections firstly decreased and then decreased,and eventually increased.The change pattern of L4 was opposite to that of L2.When the samples were classified according to age,the change pattern L value in the S1 to S4 sections was the same as that under gender classification.Conclusions The effect of gender on the L value of individuals is relatively great,while the effect of age on the L value of individuals value is very small.Setting the corresponding long axis of mandible as a reference,the change pattern of IANC in the mandible can be deduced based on the change of L value from S 1 to S4:from S1 to S4,the course of IANC on the vertical direction is firstly downward,tben turns to upward and goes upward;from S1 to S4,the trend of IANC on the horizontal direction is firstly toward the side of tongue,and eventually turns towards the buccal side.The current study provides reference data for IANC-related anatomy from the mandibular foramen to the mandibular second molars.

Objective To study astragalus polysaccharide′s synergistic enhancement on Carvedilol′s improvement effects on dilated cardiomyopathy′s heart function.Methods SD rat model of dilated cardiomyopathy was established and divided into CON group (no treatment),CAR group (only Carvedilol treatment),APS group (only astragalus polysaccharide treatment) and C+A group (Carvedilol and astragalus polysaccharide treatment) according to their treatment methods.The left ventricular ejection fraction (LVEF),Tei index,high sensitive C reactive protein (hs-CRP),tumor necrosis factor (TNF)-α,interleukin (IL)-1,IL-6,IL-8,CD3+,CD4+ and CD8+ T lymphocyte subsets in four groups were detected and compared.Results The LVEF in C+A group and CAR group were significantly higher than those in CON group and APS group respectively (P<0.05,respectively),while Tei index in C+A group and CAR group were significantly lower than those in CON group and APS group respectively (P<0.05,respectively).The LVEF in C+A group was significantly higher than that in CAR group (P<0.05),while Tei index in C+A group was significantly lower than that in CAR group (P<0.05).The hs-CRP,TNF-α,IL-1,IL-6 and IL-8 in C+A group and APS group were significantly lower than those in CON group and CAR group respectively (P<0.05,respectively),while CD3+,CD4+ and CD8+ T lymphocyte subsets in C+A group and APS group were significantly higher than those in CON group and CAR group respectively (P<0.05,respectively).The hs-CRP,TNF-α,IL-1,IL-6 and IL-8 in C+A group were significantly lower than those in APS group respectively (P<0.05,respectively),while CD3+,CD4+ and CD8+ T lymphocyte subsets in C+A group were significantly higher than those in APS group respectively (P<0.05,respectively).Conclusion Astragalus polysaccharide has significant synergistic enhancement on Carvedilol′s improvement effects on dilated cardiomyopathy′s heart function,which may be related to the enhancement of T lymphocyte immunity and the decreasing of inflammatory reaction.

Technology and technique of coronary artery stent have been developed to a certain degree, restenosis rate of metal stent reduces to 15%, but coronary artery stent is still difficult for long-term effect of interventional therapy on coronary atherosclerotic heart disease. Drug-eluting stent is newly discovered during recent years, while it is prospect for preventing restenosis postoperatively. Although clinical efficacy of drug-eluting stent is encouraged, indication is still simple. In addition, some events related to drug-eluting stent remain poorly unknown. This study was designed to compare security between drug-eluting stent and metal bared stent, analyze major factors correlated with security of drug-eluting stent, and investigate the development of drug-eluting stent.

Objective To investigate the value of interleukin-6 (IL-6) level as a prognostic indicator in diabetic ketoacidosis (DKA) patients.Methods Data of 88 cases of DKA in Sir Run Run Shaw Hospital collected from June 1,2010 to December 31,2012 were analyzed prospectively.They were divided into two groups according to the survival of patients followed up for 28 days:non-survival group (n =12) and survival group (n =76).Serum levels of IL-6,lactate,base excess and APACHE Ⅱ score in 24 hours after admission were compared between two groups by independent t test.The receiver operating characteristic (ROC) curve was plotted to analyze the performance of IL-6 as a prognostic indicator in the early stage of DKA.Correlations between IL-6 value and lactate,base excess,APACHE Ⅱ score were analyzed.Results The IL-6 values were elevated obviously in non-survival group.The ROC of IL-6 had an area under curve (AUC) of 0.783,and the cut-off value of IL-6 for the death followed up for 28 days was 448.9 ng/L.In all patients,IL-6 values and APACHE Ⅱ score showed a positive correlation between them (r =0.919,P =0.005),and also positive correlations existed between IL-6 and lactate as well as base excess (r=0.922,P=0.003; r=0.887,P=0.015)in24 hours.Conclusions IL-6 value is a reliable prognostic indicator in the early stage of DKA.

Cycloxygenase-2 plays an important role in many human tumorigenesis, and is overexpressod in breast cancer. Therefore, it is necessary to investigate the relationship between COX-2 and breast cancer, and to develop the desirable COX-2 inhibitor. These can privide a new antitumor method.

AIM:To discuss the changes of IL-17, IL-21 in glucocorticoid therapy of active thyroid associated ophthalmopathy (TAO) and analyze the relation with results.METHODS:The 67 patients (134 eyes) of TAO in our hospital, were divided into activity group(32 patients 64 eyes) and atypical activity group(35 patients 70 eyes) according clinical activity score (CAS) grading standard, and 30 cases of healthy as control group at the same time.The activity group were treated by glucocorticoid therapy treatment, and proceed the CAS scores before and after treatment, measured the degree of exophthalmus and width of palpebral fissure.At the same time, compared the expression level of IL-17 and IL-21 in all groups, and analyzed the correlation between the IL-17 and IL-21 and CAS score.RESULTS:Compared with control group, expressions of IL-17 and IL-21 in TAO patients were significantly higher (P<0.05).The expressions of IL-17 and IL-21 in active period TAO patients were higher than atypical activity (P<0.05).After glucocorticoid treatment, the expressions of IL-17 in active period TAO patients decreased significantly(P<0.05), and it was significantly positive correlation with CAS score (before treatment:r=0.8847,P=0.042;after treatment:r=0.8886,P=0.0439) the expression of IL-21 in active period TAO patients was significantly positive correlation with CAS score (before treatment:r=0.8893, P=0.0435;after treatment:r=0.8876,P=0.045).CONCLUSION:IL-17 and IL-21 is closely related to the TAO disease activity, and glucocorticoids impact treatment by reducing IL-17 and IL-21 in activity TAO, IL-17 and IL-21 can be used as one of indexes of predicted curative effect and condition in patients with TAO.

Adolescent ; Adult ; Bacterial Infections ; Female ; Humans ; Infection ; Male ; Middle Aged ; Neck ; Young Adult

Objective To evaluate the value of revised Geneva score, Daniel ECG score and age-adjusted D-dimer for predicting pulmonary embolism(PE).Methods A total of 91 cases suspected as pulmonary embolism were collected, and 52 cases were diagnosed as pulmonary embolism by computed tomographic pulmonary angiography(CTPA) results.Receiver operating characteristic(ROC) curves and diagnostic test evaluation indexes were used to evaluate the probability of PE predicted by the revised Geneva score,Daniel ECG score, age-adjusted D-dimer and combination of these two clinical scores and age-adjusted D-dimer.Results The confirmed PE was 21.4% with a low probability(revised Geneva score 0~3 points),55.4% in intermediate probability(4~10 points),85.7% in high probability(score≥11 points).The prevalence of PE was 33.3% with a low clinical probability (Daniel ECG score<2 points)and 70.7% with a high clinical probability(Daniel ECG score≥2 points).The sensitivity and specificity of age-adjusted D-dimer in predicting pulmonary embolism were 92.3%,69.2%.The area under curve of the ROC curve(AUC)in the revised Geneva score and Daniel ECG score has no significant difference(Z=0.979).The negative predictive value of the revised Geneva score, Daniel ECG score combined with D-dimer in pulmonary embolism were 100.0% and 87.5%.Conclusion All of revised Geneva score, Daniel ECG score and age-adjusted D-dimer have certain predictive value on pulmonary embolism , and the revised Geneva score combined with age-adjusted D-dimer can be more safely exclude pulmonary embolism.