OBJECTIVE:To compare the efficacy and safety of different density of Levobupivacaine for combined spinal and epidural anesthesia (CSEA) in elder patients. METHODS: Sixty elder patients undergoing transurethral prostatectomy (TURP) were randomly assigned to 1 of the 3 groups: Group Ⅰ(n=20,hypobaric group) received 0.75% Levobupivacaine 1 mL+ 0.5 mL 0.9% Sodium Chloride Injection,Group Ⅱ(n=20,isobaric group) received 0.75% Levobupivacaine 1 mL+0.5 mL cerebrospinal fluid,and Group Ⅲ(n=20,hyperbaric group) received 1 mL 0.75% Levobupivacaine+0.5 mL 10% Glucose. RESULTS: All the 3 groups achieved satisfactory anesthetic efficacy. The incidences of hypotension,nausea,vomiting and shivering were significantly lower in Group Ⅲ than in other two groups (Group Ⅰ and Group Ⅱ)(P

JM is a acute lymphocytic leukemia cell line derived from immature T lymphocytes in human thymus.We bserved the immunoregulation effect of supernatants from JM cell culture(SPN_(JM))onproliferation of human B ymphocytes and mouse spleen cells by using purified human B lymphocytesand mouse spleen cells stimulated with AC.It is showed that SPN_(JM),which suppresses T lymphocyteproliferation,enhance the proliferation response of uman B cell and mouse spleen cells stimulatedwith SAC.Without inducement of SAC,SPN_(JM) with HrIL-2 still nhances B lympohocyteproliferation.Additionally,SPN_(JM) is able to up regulate T cell proliferation response at lowconcentration(1:640)although it suppresses T cell proliferation at high concentration.

Objective To compare the level of glycosylated hemoglobin (HbA1c) in patients with diabetes treated with actrapid monotard analog or actrapid monotard. Methods One hundred and twenty patients with type 2 diabetes were divided into actrapid monotard analog group and actrapid monotard group with 60 cases in each by admission number. Fasting blood glucose (FBG), 2-hour postprandial blood glucose (2hPBG), HbA1c were obtained and analyzed. Results The overall cure rate were similar between actrapid monotard analog group and actrapid monotard group (83.3%, 50/60 vs. 86.7%, 52/60, P ＞ 0.05 ). There was no significant difference in FBG, 2hPBG before treatment between two groups (P ＞ 0.05),but there was significant difference at 2 weeks after treatment between two groups (P ＜ 0.05 ) . There was no significant difference in HbA1c before treatment between two groups (P＞ 0.05 ) ,but there was significant difference at 3 months after treatment between two groups (P ＜ 0.05 ). FBG, 2hPBG, HbA1c after treatment were improved compared with before treatment in two groups (P ＜ 0.05 ). Conclusion Actrapid monotard analog provides better blood glucose control compare with actrapid monotard while providing the convenience of injecting immediately before meals.

Plastic bronchitis is a “multiple-system” involved syndrome that is characterized by the formarion of thick,arborizing mucofibrinous tracheobronchial casts,which can result in hard breathing or pulmonary failure.Plastic bronchitis in children occurs following correction of complex cardiac defects,or in children with inflammatory pulmonary conditions.It is a condition of unknown causes and has different clinical manifestations.Early diagnosis is very important and so far endoscopic removal of the casts is the most effective measure.

OBJECTIVE:To investigate the feasibility of defined individual treatment course as evaluation index of rational use of drugs.METHODS:The doses of antibiotics and treatment course in 63 patients with type Ⅰ incision operation of our hospital in Jan.2010 were taken as examples.Case analysis,DUI evaluation and defined individual treatment course evaluation were adopted.Similar results were obtained by 3 kinds of methods.RESULTS&CONCLUSION:Results of 3 kinds of methods are consistent.DUI evaluation isn't closely associated with treatment course.Defined individual treatment course is more sensitive and available than DUI based on the introduction of dose and treatment course.

ObjectiveTo observe the lesions of psoriasis vulgaris by confocal laser scanning microscopy(CLSM).MethodsThe typical lesions of 120 patients with psoriasis vulgaris were observed by CLSM,and scanning results were recorded.ResultsOf the 120 patients with psoriasis vulgaris,all (100％)had dilated and twisted capillaries in dermal papillae,113 (94.17％) acanthosis,106 (88.33％) parakeratosis,86 (71.67％) Munro microabscesses at the level of stratum corneum,71(59.17％) diminished or disappeared stratum granulosum.There were a few(1 to 2) dilated capillary lumens in papillary dermis,with no obvious reduction in the distance between dermal papillae.Some dermal papillary rings lost their integrity or brightness slightly in 24 of the 27 patients with psoriasis guttata.The height of stratum spinosum was 96.85 ± 17.88 μm (ranged:80-150 μm) in patients with psoriasis guttata,lower than that in those with plaque psoriasis (average:143.45 ± 23.19 μm,range:100-190 μm).In lesions of plaque psoriasis,the capillaries in dermal papillae were apparently dilated with hyperemia,showing a characteristic'S' shape or horseshoe appearance; the diameter of dermal papillae was increased and the distance between dermal papillae was reduced,giving a 'transverse section of lotus roots' appearance.Dermal papillary rings completely disappeared in 25 of the 29 patients with plaque psoriasis.ConclusionCLSM may serve as an aid in the diagnosis of psoriasis vulgaris.

Objective To understand the epidemic situation,epidemiological characteristics and clinical features of import?ed malaria in Donghai County,Lianyungang City from 2005 to 2015. Methods The epidemiological data of imported malaria in Donghai County from 2005 to 2015 were collected and analyzed by the descriptive epidemiological method. Results A total of 126 imported malaria cases were reported in Donghai County from 2005 to 2015,including 101 falciparum malaria cases (80.19%),13 ovale malaria cases(10.32%),7 vivax malaria cases(5.56%),4 quartan malaria cases(3.17%),and 1 case with mixed infection(0.79%). These cases were distributed in 14 towns,and mainly in 4 towns namely Niushan,Tuofeng,Shil?iu,and Huangchuan,and the cases in the 4 towns accounted for 63.49% of the total cases. The cases occurred in the whole year,but the peak period of the disease was in April,which was related to the returned time of these patients from foreign coun?tries. All the patients were male and aged from 20 to 55 years,and those aged from 20 to 50 years accounted for 90.70%. Con?clusions The epidemic situation of imported malaria presents a rising trend in Donghai County in recent 10 years. To control imported malaria,the above?mentioned 4 towns with more migrant construction workers and businessmen should be treated as key areas. Meanwhile,the floating population management,disposal of the malaria epidemic spots,mosquito vector monitoring as well as health education should be strengthened.

Objective To estimate the association of the single nucleotide polymorphism (SNP) and the serum concentration of interleukin (IL)-18 with ulcerative colitis (UC) . Methods Samples collected from 50 patients with UC and 128 healthy controls were detected for SNPs of IL-18 gene including rs187238, rs5744228, rs360718 and rs360717 using polymerase chain reaction (PCR) and direct sequencing. Meanwhile, the serum level of IL-18 was measured. ResultsSerum concentration of IL-18 was higher in patients with UC than in healthy controls [(3029.9±111.7) pg/ml vs ( 133.2±39.4) pg/ml,(P<0.01)], rs187238 C, rs360718 C and rs360717 A were significantly related to UC (X~2=9.26, P<0.01). There was significant difference in frequencies of rs187238 C, rs360718 C and rs360717 A between patients with UC and healthy controls (X~2=9.26, P<0. 01). Serum concentration of IL-18 was higher in UC patients with C allele than in healthy controls (P<0.05).Conclusions There is correlation between SNP and serum level of IL-18 , which may involve in susceptibility to UC.

In condition of direct vision on fundus of eye, microperimeter can quantitatively detect the retinal light sensitivity of macular area, and record real time tracking of the eye, automatically analyze the location and stability of fixation. Microperimeter matches fundus image with micro cyclogram point-to-point, thus it achieves the combination of visual function and structure. The characteristics of microperimeter have good relations with subjective vision, so it can be a new method for the accurate vision evaluation and has application potential to assess the visual function in legal medicine. In this ar-ticle, we summarize the principle, method and parameters of microperimeter. Also, the applications of microperimeter in vision assessment are focused in order to provide a reference for the assessment of visual function in the legal medicine.