Lysophosphatidic acid(LPA) is a potent bioactive lipid that has multiple specific effects on vessel wall cells,blood cells,cardiac myocytes and cardiac fibroblasts.Activated platelets are the primary source of LPA in circulation,which in turn stimulate platelet aggregation.It has shown that LPA can promote the formation of atherosclerosis and thrombus under certain conditions that might aggravate cardiovascular diseases,especially ischemic heart disease.LPA can enhance endothelial permeability and cause barrier dysfunction in the early phase of coronary atherosclerosis,as well as in the late phase trigger platlet activation and lead to intra-arterial thrombus formation upon rupture of the atherosclerotic plaque,finally cause myocardial ischemia and infarction.Furthermore,LPA can stimulate cardiac myocyte hypertrophy and fibroblasts proliferation,which aggravate myocardial hypertrophy after infarction.So,LPA plays an important role in the development of ischemic heart disease.

Enterovirus A, Human ; Hand, Foot and Mouth Disease ; virology ; Humans

Objective To explore the change of serum uric acid(UA) levels in patients with multiple sclerosis(MS) and its clinical significance.Methods A quantitative enzymatic assay according to the manufacturer's protocol was adopted to detect serum UA levels in 43 patients with MS and 45 normal.Results The mean serum UA level in patients with MS was significantly lower in comparison with controls( P

Insect viruses are attractive as biological control agents and could be a feasible alternative to chemical insecticides in the management of insect infestations. This review describes recent advances in the development of wild-type and genetically modified viruses as insecticides. A new strategy of application of insect viruses in China is reviewed. Also, the assessment of biosafety of genetically modified Helicoverpa armigera Nucleopolyhedovirus (HearNPV) is emphasized as a case-study.

Background Aberrant proliferation of residual lens epithelial cells (LECs) is one of main causes of posterior capsular opacification (PCO).Researches indicated that Wnt3a signaling pathway promote proliferation of epithelial cells,but its effect on LECs is still unclear. Objective The present study was to investigate the effects of Wnt3a on proliferation of human LECs and its mechanism and to provide a new gene target in the prevention and treatment of PCO. Methods Human LECs line (SRA01/04 cells ) was cultured and then incubated to 6-well plate at the density of 4×105/well.A human Wnt3a cDNA expressing vector targeted human LECs was constructed to increase the Wnt3a expression in SRA01/04 cells,and pcDNA3-HA expression vector was used as the control group.The expression of Wnt3a was identified by Western blot assay after transfected.The growth and proliferation of SRA01/04 cells were detected by MTT and flow cytometry (FCM).The expressions of β-catenin,cyclin D1 and c-myc in the cells were detected by Western blot assay.β-Catenin expression was localized using immunofluorescence assay,and the expression and localization of proliferating cell nuclear antigen ( PCNA ) were analyzed by immunocytochemistry for the exploration of the active mechanism of Wnt3a to proliferation of LECs.Results Human Wnt3a cDNA expression vector was designed successfully and transiently transfected to SRA01/04 cells,and Wnt3a/SRA01/04 cells and pcDNA3-HA/SRA01/04 cells were obtained.The expression of Wnt3a was verified in the Wnt3a transfected group compared with the control group.MTT indicated that the cell proliferating rate was significantly different between the Wnt3a transfected group and the control group ( Fgroup =15.235,P =0.005 ;Ftime =369.677,P =0.000),and that in various time points after transfected was significantly different (t =20.843,P=0.001 ;t =26.214,P＜0.001 ;t=25.177,P=0.001 ;t =35.516,P＜0.001 ;t =615.056,P＜0.001 ).The proportion of SRA01/04 cells in G1 phase was 51.74％ in the Wnt3a cDNA transfected group,with a significantly decrease in comparison with 79.44％ of the control group.However,the proportion of SRA01/04 cells in S phase in the Wnt3a cDNA transfected group was higher than that of the control group (36.23％ versus 12.34％ ).The positive expression rate of PCNA protein in SRA01/04 was (47.00％ ±7.58％ ) in the Wnt3a cDNA transfected group and ( 16.00％ ±3.61％ ) in the control group with a significant difference between them (t =8.256,P＜0.01 ).After 48 hours of transfection of the Wnt3a cDNA,the expression amount of β-catenin proteins was higher and the immunofluorescence was stronger in cell nucleus,and the expressions of cyclin D1 and c-myc proteins were elevated in Wnt3a/SRA01/04 cells. Conclusions The overexpression of Wnt3a activates the Wnt/β-catenin signaling pathway and downregulates the expression of a subset of target genes,including cyclin D1 and c-myc,which plays an important role in promoting the proliferation of human LECs.

AIM: To investigate the clinical value of fundus fluorescein angiography ( FFA ) and optical coherent tomography ( OCT) in the diagnosis of central retinal vein occlusion.
●METHODS:A total of 47 cases (47 eyes) central retinal vein occlusion were retrospectively analyzed from Jun. 2012 to Dec. 2015 in our hospital ophthalmology center. According to the final diagnosis, the results were divided into 21 cases of central retinal artery occlusion ( group CRAO, 21 eyes) and central retinal vein occlusion ( group CRVO, 26 eyes ) . All patients received FFA and OCT examination within 2wk of onset, and the image data of the two kinds of examination results were analyzed.
● RESULTS: Group of patients with CRAO average macular foveola thickness, angle measuring, filling time determination results were significantly lower than that of the patients with CRVO group average and the differences were significant (P<0. 05).
●CONCLUSION:FFA and OCT images of central retinal artery and vein occlusion have their own characteristics, and the combination of these two images can be used to identify and diagnose the central retinal artery and vein occlusion.

To investigate the angiotensin I-converting enzyme (ACE) inhibitory activity of beta-chain hemoglobin fragments, 17 fragments were synthesized by microwave-assisted solid-phase synthesis method. Wang resin or Trt(2-Cl) resin, Fmoc and HBTU-HOBt were used as solid carrier, N-terminal amino acid protecting groups and coupling reagents, respectively. The ACE inhibitory, alpha-glucosidase inhibitory, antibacterial and antitumor activities of the synthesized fragments were assayed. In vitro, Val-Val-Tyr-Pro-Trp-Thr showed high ACE inhibitory activity (IC50 = 7.42 micromol x L(-1)). The results indicate that there are two active sites in Val-Val-Tyr-Pro-Trp-Thr-Gln-Arg-Phe, one consists of Val-Val-, and the other -Gln-Arg-Phe. Peptides showed high ACE inhibitory activity when the N-terminal was hydrophobic amino acid such as Val and C-terminal tripeptide contained Phe, Trp or Arg. Some of the fragments showed low a-glucosidase inhibitory activity. No antibacterial activity or antitumor activity was detected in vitro. The results indicate that these peptides have a potential antihypertensive effect and possible application in the treatment of hypertension.

Aged ; Aged, 80 and over ; Cross Infection ; etiology ; Diabetes Mellitus, Type 2 ; complications ; microbiology ; Humans ; Male ; Middle Aged ; Pneumoconiosis ; complications ; microbiology ; Retrospective Studies

Humans ; Hyperthyroidism ; Infant, Newborn